Restriction map for
pFN21AB6107
(Circular) (Six-base) MAPSORT of: pFN21AB6107.seq Check: 8714 from: 1 to: 6445
pFN21AB6107 6445 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4255 278
Size: 53 83 186 936 2719 2468
Fragments arranged by size:
2719 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3342 3788 1937
Size: 1405 446 4594
Fragments arranged by size:
4594 1405 446
Acc65I G'GTAC_C
Cuts at: 2622 3766 2622
Size: 1144 5301
AclI AA'CG_TT
Cuts at: 1563 4572 4945 1563
Size: 3009 373 3063
Fragments arranged by size:
3063 3009 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2504 3510 4508 5892 6160 1632
Size: 872 1006 998 1384 268 1917
Fragments arranged by size:
1917 1384 1006 998 872 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6248
AflIII A'CryG_T
Cuts at: 1203 5344 6321 1203
Size: 4141 977 1327
Fragments arranged by size:
4141 1327 977
AgeI A'CCGG_T
Cuts at: 2646 2712 5276 2646
Size: 66 2564 3815
Fragments arranged by size:
3815 2564 66
AhdI GACnn_n'nnGTC
Cuts at: 2113 5174 2113
Size: 3061 3384
AleI CACnn'nnGTG
Cuts at: 1166 2918 1166
Size: 1752 4693
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6445
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3080 3112 3080
Size: 32 6413
AlwNI CAG_nnn'CTG
Cuts at: 2419 3715 4188 5760 2419
Size: 1296 473 1572 3104
Fragments arranged by size:
3104 1572 1296 473
ApaI G_GGCC'C
Cuts at: 2080 2613 2753 2080
Size: 533 140 5772
Fragments arranged by size:
5772 533 140
ApaLI G'TGCA_C
Cuts at: 4502 5658 4502
Size: 1156 5289
ApoI r'AATT_y
Cuts at: 1493 3149 3291 3754 3980 4132 6241 1493
Size: 1656 142 463 226 152 2109 1697
Fragments arranged by size:
2109 1697 1656 463 226 152 142
AseI AT'TA_AT
Cuts at: 160 1032 4999 160
Size: 872 3967 1606
Fragments arranged by size:
3967 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6445
AvaI C'yCGr_G
Cuts at: 1870 1943 2034 2174 2543 2552 3533 3770 1870
Size: 73 91 140 369 9 981 237 4545
Fragments arranged by size:
4545 981 369 237 140 91 73 9
AvrII C'CTAG_G
Cuts at: 2311 2311
Size: 6445
BamHI G'GATC_C
Cuts at: 2023 3775 4142 2023
Size: 1752 367 4326
Fragments arranged by size:
4326 1752 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2048 2492 2622 3766 5221
Size: 325 217 627 261 444 130 1144 1455
Cuts at: 5221 618
Size: 1842
Fragments arranged by size:
1842 1455 1144 627 444 325 261 217
130
BanII G_rGCy'C
Cuts at: 1391 2020 2080 2295 2553 2613 2697 2753 2889
Size: 629 60 215 258 60 84 56 136
Cuts at: 2889 3764 1391
Size: 875 4072
Fragments arranged by size:
4072 875 629 258 215 136 84 60
60 56
BbeI G_GCGC'C
Cuts at: 2496 2496
Size: 6445
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2324 928
Size: 971 425 5049
Fragments arranged by size:
5049 971 425
BbvCI CC'TCA_GC
Cuts at: 3235 3235
Size: 6445
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4636 4670 1903
Size: 34 2699 34 3678
Fragments arranged by size:
3678 2699 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4337 5547 4337
Size: 1210 5235
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6445
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5056 136
Size: 107 122 71 631 605 3384 1525
Fragments arranged by size:
3384 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6440 6440
Size: 6445
BlpI GC'TnA_GC
Cuts at: 3480 3862 3480
Size: 382 6063
Bme1580I G_kGCm'C
Cuts at: 1790 2080 2613 2753 4506 5662 1790
Size: 290 533 140 1753 1156 2573
Fragments arranged by size:
2573 1753 1156 533 290 140
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6445
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2254 2674 2890
Size: 416 53 83 399 24 837 420 216
Cuts at: 2890 2912 3213 4176 5134 442
Size: 22 301 963 958 1753
Fragments arranged by size:
1753 963 958 837 420 416 399 301
216 83 53 24 22
BmtI G_CTAG'C
Cuts at: 1056 3267 1056
Size: 2211 4234
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2578 2578
Size: 6445
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3262 3385 5105 1239
Size: 2023 123 1720 2579
Fragments arranged by size:
2579 2023 1720 123
Bpu10I CC'TnA_GC
Cuts at: 3235 3316 3235
Size: 81 6364
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3593 4570 5435 5733 3593
Size: 977 865 298 4305
Fragments arranged by size:
4305 977 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2703 5108 882
Size: 617 1204 2405 2219
Fragments arranged by size:
2405 2219 1204 617
BsaAI yAC'GTr
Cuts at: 493 1204 4236 493
Size: 711 3032 2702
Fragments arranged by size:
3032 2702 711
BsaBI GATnn'nnATC
Cuts at: 2345 6439 2345
Size: 4094 2351
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2493 4252 4634 5269
Size: 53 83 186 936 960 1759 382 635
Cuts at: 5269 275
Size: 1451
Fragments arranged by size:
1759 1451 960 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2646 2712 4878 5276 5550 5697 6108 2646
Size: 66 2166 398 274 147 411 2983
Fragments arranged by size:
2983 2166 411 398 274 147 66
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2120 2150 2282 2312 2540
Size: 30 165 30 717 30 132 30 228
Cuts at: 2540 2570 2925 2955 3080 3110 1178
Size: 30 355 30 125 30 4513
Fragments arranged by size:
4513 717 355 228 165 132 125 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2303 2561 2867 2933 3088 1186
Size: 1117 258 306 66 155 4543
Fragments arranged by size:
4543 1117 306 258 155 66
BseYI C'CCAG_C
Cuts at: 3641 5648 6144 3641
Size: 2007 496 3942
Fragments arranged by size:
3942 2007 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2659 2671 3534 3657 1163
Size: 573 923 12 863 123 3951
Fragments arranged by size:
3951 923 863 573 123 12
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3895 4656 5684 1540
Size: 275 186 1894 761 1028 2301
Fragments arranged by size:
2301 1894 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2020 2553 2889 3248 4506 4591 5662 2020
Size: 533 336 359 1258 85 1071 2803
Fragments arranged by size:
2803 1258 1071 533 359 336 85
BsmI GAATG_Cn'
Cuts at: 3956 4049 3956
Size: 93 6352
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2020 2080 2295 2553 2613 2697 2753
Size: 399 230 60 215 258 60 84 56
Cuts at: 2753 2889 3248 3764 4506 4591 5662 1391
Size: 136 359 516 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 516 399 359 258 230
215 136 85 84 60 60 56
BspEI T'CCGG_A
Cuts at: 6108 6108
Size: 6445
BspHI T'CATG_A
Cuts at: 4334 6064 4334
Size: 1730 4715
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2634 3800 844
Size: 1790 1166 3489
Fragments arranged by size:
3489 1790 1166
BsrBI CCG'CTC
Cuts at: 1394 1823 3538 4332 1394
Size: 429 1715 794 3507
Fragments arranged by size:
3507 1715 794 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2594 2645 4940 5114 65
Size: 1183 1346 51 2295 174 1396
Fragments arranged by size:
2295 1396 1346 1183 174 51
BsrFI r'CCGG_y
Cuts at: 1700 1810 2148 2273 2646 2712 2970 5089 5276
Size: 110 338 125 373 66 258 2119 187
Cuts at: 5276 1700
Size: 2869
Fragments arranged by size:
2869 2119 373 338 258 187 125 110
66
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6445
BssHII G'CGCG_C
Cuts at: 1925 2478 1925
Size: 553 5892
BssSI C'ACGA_G
Cuts at: 2216 2666 4505 5517 2216
Size: 450 1839 1012 3144
Fragments arranged by size:
3144 1839 1012 450
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2307 2682 794
Size: 1513 375 4557
Fragments arranged by size:
4557 1513 375
BstBI TT'CG_AA
Cuts at: 2226 5249 2226
Size: 3023 3422
BstEII G'GTnAC_C
Cuts at: 1184 2369 1184
Size: 1185 5260
BstYI r'GATC_y
Cuts at: 1972 2023 2340 3775 4142 4527 4544 5996 6082
Size: 51 317 1435 367 385 17 1452 86
Cuts at: 6082 6094 6440 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1435 385 367 346 317 86
51 17 12
Bsu36I CC'TnA_GG
Cuts at: 3696 3696
Size: 6445
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4954
Fragments arranged by size:
4954 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2482 2555 2794 487
Size: 683 56 1256 73 239 4138
Fragments arranged by size:
4138 1256 683 239 73 56
BtsI GCAGTG_nn'
Cuts at: 2387 2410 2663 3517 3961 4755 4775 2387
Size: 23 253 854 444 794 20 4057
Fragments arranged by size:
4057 854 794 444 253 23 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6445
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6410
DraI TTT'AAA
Cuts at: 3303 3749 3303
Size: 446 5999
DraIII CAC_nnn'GTG
Cuts at: 2682 2682
Size: 6445
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5452 809
Size: 507 4136 1802
Fragments arranged by size:
4136 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3539 3892 4781
Size: 54 1425 50 275 18 1709 353 889
Cuts at: 4781 6410 8
Size: 1629 43
Fragments arranged by size:
1709 1629 1425 889 353 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3892 1537
Size: 275 2080 4090
Fragments arranged by size:
4090 2080 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4375 1353
Size: 3022 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3005 5032 5406 5552 387
Size: 1064 138 1416 2027 374 146 1280
Fragments arranged by size:
2027 1416 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2018 2551 2887 2018
Size: 533 336 5576
Fragments arranged by size:
5576 533 336
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2504 3262 3385 3510 4508 5105 5892
Size: 393 872 758 123 125 998 597 787
Cuts at: 5892 6160 1239
Size: 268 1524
Fragments arranged by size:
1524 998 872 787 758 597 393 268
125 123
EcoNI CCTnn'n_nnAGG
Cuts at: 2084 2255 3066 2084
Size: 171 811 5463
Fragments arranged by size:
5463 811 171
EcoO109I rG'GnC_Cy
Cuts at: 2076 2077 2248 2749 2076
Size: 1 171 501 5772
Fragments arranged by size:
5772 501 171 1
EcoRI G'AATT_C
Cuts at: 3149 3754 4132 3149
Size: 605 378 5462
Fragments arranged by size:
5462 605 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2116 2857 3028 2116
Size: 741 171 5533
Fragments arranged by size:
5533 741 171
FspI TGC'GCA
Cuts at: 3023 4951 3023
Size: 1928 4517
HaeII r_GCGC'y
Cuts at: 1678 2496 3096 5592 1678
Size: 818 600 2496 2531
Fragments arranged by size:
2531 2496 818 600
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2099 2131 2433 2465 2925
Size: 32 135 32 527 32 302 32 460
Cuts at: 2925 2957 3218 3250 3329 3361 3578 3610 5086
Size: 32 261 32 79 32 217 32 1476
Cuts at: 5086 5118 5160 5192 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1476 527 460 302 261 217 135
79 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2872 3343 3789 4035 669
Size: 988 281 934 471 446 246 3079
Fragments arranged by size:
3079 988 934 471 446 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6445
HpaI GTT'AAC
Cuts at: 4035 4035
Size: 6445
KasI G'GCGC_C
Cuts at: 2492 2492
Size: 6445
KpnI G_GTAC'C
Cuts at: 2626 3770 2626
Size: 1144 5301
MfeI C'AATT_G
Cuts at: 4044 4044
Size: 6445
MluI A'CGCG_T
Cuts at: 6321 6321
Size: 6445
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5559 5743 1305
Size: 4254 184 2007
Fragments arranged by size:
4254 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4623
Fragments arranged by size:
4623 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2274 2918 4403 4762 4921 518
Size: 648 1108 644 1485 359 159 2042
Fragments arranged by size:
2042 1485 1108 648 644 359 159
MspA1I CmG'CkG
Cuts at: 1606 2332 3479 3641 3861 4538 5686 5931 1606
Size: 726 1147 162 220 677 1148 245 2120
Fragments arranged by size:
2120 1148 1147 726 677 245 220 162
NarI GG'CG_CC
Cuts at: 2493 2493
Size: 6445
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4954
Fragments arranged by size:
4954 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6445
NheI G'CTAG_C
Cuts at: 1052 3263 1052
Size: 2211 4234
NotI GC'GGCC_GC
Cuts at: 3892 3892
Size: 6445
NspI r_CATG'y
Cuts at: 1132 1229 2273 3454 3803 5348 1132
Size: 97 1044 1181 349 1545 2229
Fragments arranged by size:
2229 1545 1181 1044 349 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6445
PciI A'CATG_T
Cuts at: 5344 5344
Size: 6445
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2244 3259 3676 1108
Size: 1136 1015 417 3877
Fragments arranged by size:
3877 1136 1015 417
PfoI T'CCnGG_A
Cuts at: 2281 2281
Size: 6445
PmeI GTTT'AAAC
Cuts at: 3749 3749
Size: 6445
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3080 3112 4514 4546 6027 6059 3080
Size: 32 1402 32 1481 32 3466
Fragments arranged by size:
3466 1481 1402 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2248 2248
Size: 6445
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6445
PsiI TTA'TAA
Cuts at: 2964 4015 2964
Size: 1051 5394
PspOMI G'GGCC_C
Cuts at: 2076 2609 2749 2076
Size: 533 140 5772
Fragments arranged by size:
5772 533 140
PspXI vC'TCGA_Gb
Cuts at: 2552 2552
Size: 6445
PstI C_TGCA'G
Cuts at: 830 2514 2895 3797 830
Size: 1684 381 902 3478
Fragments arranged by size:
3478 1684 902 381
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6445
PvuII CAG'CTG
Cuts at: 3479 3641 3479
Size: 162 6283
SacI G_AGCT'C
Cuts at: 2020 2553 2889 2020
Size: 533 336 5576
Fragments arranged by size:
5576 533 336
SalI G'TCGA_C
Cuts at: 1936 3341 3787 1936
Size: 1405 446 4594
Fragments arranged by size:
4594 1405 446
SbfI CC_TGCA'GG
Cuts at: 3797 3797
Size: 6445
ScaI AGT'ACT
Cuts at: 2840 4693 6405 2840
Size: 1853 1712 2880
Fragments arranged by size:
2880 1853 1712
SexAI A'CCwGG_T
Cuts at: 3669 3714 3669
Size: 45 6400
SfcI C'TryA_G
Cuts at: 826 1045 2510 2891 3793 4928 5609 5800 826
Size: 219 1465 381 902 1135 681 191 1471
Fragments arranged by size:
1471 1465 1135 902 681 381 219 191
SfoI GGC'GCC
Cuts at: 2494 2494
Size: 6445
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6445
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6445
SmaI CCC'GGG
Cuts at: 1872 3772 1872
Size: 1900 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2552 3608 4549 5450 5712 820
Size: 197 926 609 1056 941 901 262 1553
Fragments arranged by size:
1553 1056 941 926 901 609 262 197
SnaBI TAC'GTA
Cuts at: 493 4236 493
Size: 3743 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6445
SphI G_CATG'C
Cuts at: 1132 2273 3803 1132
Size: 1141 1530 3774
Fragments arranged by size:
3774 1530 1141
SspI AAT'ATT
Cuts at: 5 52 4369 5
Size: 47 4317 2081
Fragments arranged by size:
4317 2081 47
StuI AGG'CCT
Cuts at: 1517 2672 1517
Size: 1155 5290
StyI C'CwwG_G
Cuts at: 513 1065 2004 2311 2429 3441 3884 513
Size: 552 939 307 118 1012 443 3074
Fragments arranged by size:
3074 1012 939 552 443 307 118
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2693 2773 3052 4472 4489 4642 4827
Size: 1255 1113 80 279 1420 17 153 185
Cuts at: 4827 5272 325
Size: 445 1498
Fragments arranged by size:
1498 1420 1255 1113 445 279 185 153
80 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2166 2757 2838
Size: 274 80 33 51 1443 189 591 81
Cuts at: 2838 4691 6403 96
Size: 1853 1712 138
Fragments arranged by size:
1853 1712 1443 591 274 189 138 81
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2282 2314 2540 2572 6405 6437 2282
Size: 32 226 32 3833 32 2290
Fragments arranged by size:
3833 2290 226 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6445
XbaI T'CTAG_A
Cuts at: 3781 3781
Size: 6445
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3256 3409 3256
Size: 153 6292
XhoI C'TCGA_G
Cuts at: 1943 2552 1943
Size: 609 5836
XmaI C'CCGG_G
Cuts at: 1870 3770 1870
Size: 1900 4545
XmnI GAAnn'nnTTC
Cuts at: 2099 4574 2099
Size: 2475 3970
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4253 276
Size: 53 83 186 936 2719 2468
Fragments arranged by size:
2719 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI
SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI BaeI BfrBI BsiWI BsmBI BstXI
BstZ17I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI
PacI PmlI PsrI RsrII SacII SanDI SapI SfiI
SrfI SwaI