Restriction map for
pFN21AB6104
(Circular) (Six-base) MAPSORT of: pFN21AB6104.seq Check: 4686 from: 1 to: 6547
pFN21AB6104 6547 bp
With 182 enzymes: SgfI *
January 7, 2013 17:02 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2667 4357 278
Size: 53 83 186 936 1131 1690 2468
Fragments arranged by size:
2468 1690 1131 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3890 1937
Size: 1953 4594
Acc65I G'GTAC_C
Cuts at: 3868 3868
Size: 6547
AclI AA'CG_TT
Cuts at: 1563 4674 5047 1563
Size: 3111 373 3063
Fragments arranged by size:
3111 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2591 3498 4610 5994 6262 1632
Size: 959 907 1112 1384 268 1917
Fragments arranged by size:
1917 1384 1112 959 907 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6350
AflIII A'CryG_T
Cuts at: 1203 2203 3414 5446 6423 1203
Size: 1000 1211 2032 977 1327
Fragments arranged by size:
2032 1327 1211 1000 977
AgeI A'CCGG_T
Cuts at: 5378 5378
Size: 6547
AhdI GACnn_n'nnGTC
Cuts at: 3570 5276 3570
Size: 1706 4841
AleI CACnn'nnGTG
Cuts at: 1166 2313 1166
Size: 1147 5400
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6547
AlwNI CAG_nnn'CTG
Cuts at: 2128 4290 5862 2128
Size: 2162 1572 2813
Fragments arranged by size:
2813 2162 1572
ApaLI G'TGCA_C
Cuts at: 4604 5760 4604
Size: 1156 5391
ApoI r'AATT_y
Cuts at: 1493 2017 2761 2837 3856 4082 4234 6343 1493
Size: 524 744 76 1019 226 152 2109 1697
Fragments arranged by size:
2109 1697 1019 744 524 226 152 76
AseI AT'TA_AT
Cuts at: 160 1032 5101 160
Size: 872 4069 1606
Fragments arranged by size:
4069 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6547
AvaI C'yCGr_G
Cuts at: 1870 1943 3163 3189 3366 3505 3872 1870
Size: 73 1220 26 177 139 367 4545
Fragments arranged by size:
4545 1220 367 177 139 73 26
BamHI G'GATC_C
Cuts at: 3877 4244 3877
Size: 367 6180
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2382 2895 3868 5323 618
Size: 325 217 627 595 513 973 1455 1842
Fragments arranged by size:
1842 1455 973 627 595 513 325 217
BanII G_rGCy'C
Cuts at: 1391 3512 3866 1391
Size: 2121 354 4072
Fragments arranged by size:
4072 2121 354
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3213 3489 928
Size: 971 1314 276 3986
Fragments arranged by size:
3986 1314 971 276
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2260 2294 4738 4772 1903
Size: 34 323 34 2444 34 3678
Fragments arranged by size:
3678 2444 323 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4439 5649 4439
Size: 1210 5337
BclI T'GATC_A
Cuts at: 1548 2301 2346 1548
Size: 753 45 5749
Fragments arranged by size:
5749 753 45
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2462 5158 136
Size: 107 122 71 631 605 790 2696 1525
Fragments arranged by size:
2696 1525 790 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6542 6542
Size: 6547
BlpI GC'TnA_GC
Cuts at: 3964 3964
Size: 6547
Bme1580I G_kGCm'C
Cuts at: 1790 2900 4608 5764 1790
Size: 1110 1708 1156 2573
Fragments arranged by size:
2573 1708 1156 1110
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6547
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4278 5236 442
Size: 416 53 83 399 24 2861 958 1753
Fragments arranged by size:
2861 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6547
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2893 3011 3202 3395 5207 1239
Size: 1654 118 191 193 1812 2579
Fragments arranged by size:
2579 1812 1654 193 191 118
Bpu10I CC'TnA_GC
Cuts at: 2150 2150
Size: 6547
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3628 4672 5537 5835 3628
Size: 1044 865 298 4340
Fragments arranged by size:
4340 1044 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5210 882
Size: 617 3711 2219
Fragments arranged by size:
3711 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4338 493
Size: 711 3134 2702
Fragments arranged by size:
3134 2702 711
BsaBI GATnn'nnATC
Cuts at: 6541 6541
Size: 6547
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2664 4354 4736 5371
Size: 53 83 186 936 1131 1690 382 635
Cuts at: 5371 275
Size: 1451
Fragments arranged by size:
1690 1451 1131 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2982 4980 5378 5652 5799 6210 2982
Size: 1998 398 274 147 411 3319
Fragments arranged by size:
3319 1998 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3200 3230 1178
Size: 30 165 30 1797 30 4495
Fragments arranged by size:
4495 1797 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2441 1186
Size: 1255 5292
BseYI C'CCAG_C
Cuts at: 3747 5750 6246 3747
Size: 2003 496 4048
Fragments arranged by size:
4048 2003 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2339 1163
Size: 573 603 5371
Fragments arranged by size:
5371 603 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3077 3997 4758 5786 1540
Size: 275 186 1076 920 761 1028 2301
Fragments arranged by size:
2301 1076 1028 920 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2421 3512 4608 4693 5764 2421
Size: 1091 1096 85 1071 3204
Fragments arranged by size:
3204 1096 1091 1071 85
BsmI GAATG_Cn'
Cuts at: 4058 4151 4058
Size: 93 6454
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2421 2900 3512 3866 4608 4693 5764
Size: 399 631 479 612 354 742 85 1071
Cuts at: 5764 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 631 612 479 399 354
85
BspEI T'CCGG_A
Cuts at: 2982 6210 2982
Size: 3228 3319
BspHI T'CATG_A
Cuts at: 3820 4436 6166 3820
Size: 616 1730 4201
Fragments arranged by size:
4201 1730 616
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2154 3257 3647 3902 844
Size: 1310 1103 390 255 3489
Fragments arranged by size:
3489 1310 1103 390 255
BsrBI CCG'CTC
Cuts at: 1394 1823 4434 1394
Size: 429 2611 3507
Fragments arranged by size:
3507 2611 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3651 5042 5216 65
Size: 1183 2403 1391 174 1396
Fragments arranged by size:
2403 1396 1391 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3312 5191 5378 1700
Size: 110 1502 1879 187 2869
Fragments arranged by size:
2869 1879 1502 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6547
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6547
BssSI C'ACGA_G
Cuts at: 4607 5619 4607
Size: 1012 5535
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2058 2453 3738 794
Size: 1264 395 1285 3603
Fragments arranged by size:
3603 1285 1264 395
BstBI TT'CG_AA
Cuts at: 5351 5351
Size: 6547
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6547
BstXI CCAn_nnnn'nTGG
Cuts at: 2174 2253 2174
Size: 79 6468
BstYI r'GATC_y
Cuts at: 1972 3370 3811 3877 4244 4629 4646 6098 6184
Size: 1398 441 66 367 385 17 1452 86
Cuts at: 6184 6196 6542 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1398 441 385 367 346 86
66 17 12
Bsu36I CC'TnA_GG
Cuts at: 3286 3286
Size: 6547
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5995
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2700 487
Size: 683 56 1474 4334
Fragments arranged by size:
4334 1474 683 56
BtsI GCAGTG_nn'
Cuts at: 2059 3100 3229 3672 3758 4063 4857 4877 2059
Size: 1041 129 443 86 305 794 20 3729
Fragments arranged by size:
3729 1041 794 443 305 129 86 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6547
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6512
DraI TTT'AAA
Cuts at: 3851 3851
Size: 6547
DraIII CAC_nnn'GTG
Cuts at: 2895 2895
Size: 6547
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5554 809
Size: 507 4238 1802
Fragments arranged by size:
4238 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3538 3700 3994
Size: 54 1425 50 275 18 1708 162 294
Cuts at: 3994 4883 6512 8
Size: 889 1629 43
Fragments arranged by size:
1708 1629 1425 889 294 275 162 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3994 1537
Size: 275 2182 4090
Fragments arranged by size:
4090 2182 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2766 2997 3315 3384 3517 3561 4477 1353
Size: 1413 231 318 69 133 44 916 3423
Fragments arranged by size:
3423 1413 916 318 231 133 69 44
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5134 5508 5654 387
Size: 1064 138 3545 374 146 1280
Fragments arranged by size:
3545 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3510 3510
Size: 6547
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2591 2893 3011 3202 3395 3498 4610
Size: 393 959 302 118 191 193 103 1112
Cuts at: 4610 5207 5994 6262 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1112 959 787 597 393 302 268
193 191 118 103
EcoRI G'AATT_C
Cuts at: 3856 4234 3856
Size: 378 6169
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2645 2645
Size: 6547
FspI TGC'GCA
Cuts at: 3650 5053 3650
Size: 1403 5144
HaeII r_GCGC'y
Cuts at: 1678 3121 5694 1678
Size: 1443 2573 2531
Fragments arranged by size:
2573 2531 1443
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2101 2133 3200 3232 3556
Size: 32 135 32 529 32 1067 32 324
Cuts at: 3556 3588 5188 5220 5262 5294 1373
Size: 32 1600 32 42 32 2626
Fragments arranged by size:
2626 1600 1067 529 324 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3891 4137 669
Size: 988 281 1953 246 3079
Fragments arranged by size:
3079 1953 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2228 3530 748
Size: 1480 1302 3765
Fragments arranged by size:
3765 1480 1302
HpaI GTT'AAC
Cuts at: 4137 4137
Size: 6547
KpnI G_GTAC'C
Cuts at: 3872 3872
Size: 6547
MfeI C'AATT_G
Cuts at: 4146 4146
Size: 6547
MluI A'CGCG_T
Cuts at: 6423 6423
Size: 6547
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2677 2791 3554 5661 5845 1305
Size: 1372 114 763 2107 184 2007
Fragments arranged by size:
2107 2007 1372 763 184 114
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3540 3702 10
Size: 54 1425 343 1708 162 2855
Fragments arranged by size:
2855 1708 1425 343 162 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2313 3443 4505 4864 5023 518
Size: 648 1147 1130 1062 359 159 2042
Fragments arranged by size:
2042 1147 1130 1062 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3064 3087 3963 4640 5788 6033 1606
Size: 1458 23 876 677 1148 245 2120
Fragments arranged by size:
2120 1458 1148 876 677 245 23
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5995
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6547
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6547
NotI GC'GGCC_GC
Cuts at: 3994 3994
Size: 6547
NspI r_CATG'y
Cuts at: 1132 1229 2207 2590 3418 3442 3905 5450 1132
Size: 97 978 383 828 24 463 1545 2229
Fragments arranged by size:
2229 1545 978 828 463 383 97 24
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6547
PciI A'CATG_T
Cuts at: 2203 3414 5446 2203
Size: 1211 2032 3304
Fragments arranged by size:
3304 2032 1211
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2128 3709 1108
Size: 1020 1581 3946
Fragments arranged by size:
3946 1581 1020
PmeI GTTT'AAAC
Cuts at: 3851 3851
Size: 6547
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4616 4648 6129 6161 4616
Size: 32 1481 32 5002
Fragments arranged by size:
5002 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6547
PsiI TTA'TAA
Cuts at: 4117 4117
Size: 6547
PspXI vC'TCGA_Gb
Cuts at: 3505 3505
Size: 6547
PstI C_TGCA'G
Cuts at: 830 2463 2964 3254 3899 830
Size: 1633 501 290 645 3478
Fragments arranged by size:
3478 1633 645 501 290
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6547
PvuII CAG'CTG
Cuts at: 3064 3064
Size: 6547
SacI G_AGCT'C
Cuts at: 3512 3512
Size: 6547
SalI G'TCGA_C
Cuts at: 1936 3889 1936
Size: 1953 4594
SapI GCTCTTCn'nnn_
Cuts at: 3517 3517
Size: 6547
SbfI CC_TGCA'GG
Cuts at: 2463 3899 2463
Size: 1436 5111
ScaI AGT'ACT
Cuts at: 2324 3547 4795 6507 2324
Size: 1223 1248 1712 2364
Fragments arranged by size:
2364 1712 1248 1223
SfcI C'TryA_G
Cuts at: 826 1045 2459 2680 2960 3250 3895 5030 5711
Size: 219 1414 221 280 290 645 1135 681
Cuts at: 5711 5902 826
Size: 191 1471
Fragments arranged by size:
1471 1414 1135 681 645 290 280 221
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6547
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6547
SmaI CCC'GGG
Cuts at: 1872 3191 3368 3874 1872
Size: 1319 177 506 4545
Fragments arranged by size:
4545 1319 506 177
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3505 3607 4651 5552 5814 820
Size: 197 926 1562 102 1044 901 262 1553
Fragments arranged by size:
1562 1553 1044 926 901 262 197 102
SnaBI TAC'GTA
Cuts at: 493 4338 493
Size: 3845 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6547
SphI G_CATG'C
Cuts at: 1132 3905 1132
Size: 2773 3774
SspI AAT'ATT
Cuts at: 5 52 2240 4471 5
Size: 47 2188 2231 2081
Fragments arranged by size:
2231 2188 2081 47
StuI AGG'CCT
Cuts at: 1517 2432 3723 1517
Size: 915 1291 4341
Fragments arranged by size:
4341 1291 915
StyI C'CwwG_G
Cuts at: 513 1065 3986 513
Size: 552 2921 3074
Fragments arranged by size:
3074 2921 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4574 4591 4744 4929 5374 325
Size: 1255 2994 17 153 185 445 1498
Fragments arranged by size:
2994 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2322 3545 4793
Size: 274 80 33 51 1443 345 1223 1248
Cuts at: 4793 6505 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1248 1223 345 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2805 2837 3137 3169 6507 6539 2805
Size: 32 300 32 3338 32 2813
Fragments arranged by size:
3338 2813 300 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6547
XbaI T'CTAG_A
Cuts at: 3883 3883
Size: 6547
XhoI C'TCGA_G
Cuts at: 1943 3505 1943
Size: 1562 4985
XmaI C'CCGG_G
Cuts at: 1870 3189 3366 3872 1870
Size: 1319 177 506 4545
Fragments arranged by size:
4545 1319 506 177
XmnI GAAnn'nnTTC
Cuts at: 3641 4676 3641
Size: 1035 5512
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2665 4355 276
Size: 53 83 186 936 1131 1690 2468
Fragments arranged by size:
2468 1690 1131 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I EcoNI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PsrI RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI