Restriction map for
pFN21AB6103
(Circular) (Six-base) MAPSORT of: pFN21AB6103.seq Check: 9542 from: 1 to: 6436
pFN21AB6103 6436 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4246 278
Size: 53 83 186 936 2710 2468
Fragments arranged by size:
2710 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3109 3779 1937
Size: 1172 670 4594
Fragments arranged by size:
4594 1172 670
Acc65I G'GTAC_C
Cuts at: 3757 3757
Size: 6436
AclI AA'CG_TT
Cuts at: 1563 2406 4563 4936 1563
Size: 843 2157 373 3063
Fragments arranged by size:
3063 2157 843 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2319 2421 3390 4499 5883 6151 1632
Size: 687 102 969 1109 1384 268 1917
Fragments arranged by size:
1917 1384 1109 969 687 268 102
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6239
AflIII A'CryG_T
Cuts at: 1203 5335 6312 1203
Size: 4132 977 1327
Fragments arranged by size:
4132 1327 977
AgeI A'CCGG_T
Cuts at: 5267 5267
Size: 6436
AhdI GACnn_n'nnGTC
Cuts at: 5165 5165
Size: 6436
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6436
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6436
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2220 2252 2220
Size: 32 6404
AlwNI CAG_nnn'CTG
Cuts at: 2666 4179 5751 2666
Size: 1513 1572 3351
Fragments arranged by size:
3351 1572 1513
ApaI G_GGCC'C
Cuts at: 3607 3607
Size: 6436
ApaLI G'TGCA_C
Cuts at: 4493 5649 4493
Size: 1156 5280
ApoI r'AATT_y
Cuts at: 1493 2474 3061 3140 3474 3617 3745 3971 4123
Size: 981 587 79 334 143 128 226 152
Cuts at: 4123 6232 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 981 587 334 226 152 143
128 79
AseI AT'TA_AT
Cuts at: 160 1032 2513 3677 4990 160
Size: 872 1481 1164 1313 1606
Fragments arranged by size:
1606 1481 1313 1164 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6436
AvaI C'yCGr_G
Cuts at: 1870 1943 3761 1870
Size: 73 1818 4545
Fragments arranged by size:
4545 1818 73
BamHI G'GATC_C
Cuts at: 3413 3766 4133 3413
Size: 353 367 5716
Fragments arranged by size:
5716 367 353
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3757 5212 618
Size: 325 217 627 1970 1455 1842
Fragments arranged by size:
1970 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3607 3755 1391
Size: 2216 148 4072
Fragments arranged by size:
4072 2216 148
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2034 928
Size: 971 135 5330
Fragments arranged by size:
5330 971 135
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2537 2571 4627 4661 1903
Size: 34 600 34 2056 34 3678
Fragments arranged by size:
3678 2056 600 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4328 5538 4328
Size: 1210 5226
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6436
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5047 136
Size: 107 122 71 631 605 3375 1525
Fragments arranged by size:
3375 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6431 6431
Size: 6436
BlpI GC'TnA_GC
Cuts at: 3853 3853
Size: 6436
Bme1580I G_kGCm'C
Cuts at: 1790 3607 4497 5653 1790
Size: 1817 890 1156 2573
Fragments arranged by size:
2573 1817 1156 890
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6436
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3601 4167 5125
Size: 416 53 83 399 24 2184 566 958
Cuts at: 5125 442
Size: 1753
Fragments arranged by size:
2184 1753 958 566 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6436
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2060 5096 1239
Size: 821 3036 2579
Fragments arranged by size:
3036 2579 821
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4561 5426 5724 4561
Size: 865 298 5273
Fragments arranged by size:
5273 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2532 5099 882
Size: 617 1033 2567 2219
Fragments arranged by size:
2567 2219 1033 617
BsaAI yAC'GTr
Cuts at: 493 1204 4227 493
Size: 711 3023 2702
Fragments arranged by size:
3023 2702 711
BsaBI GATnn'nnATC
Cuts at: 6430 6430
Size: 6436
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4243 4625 5260 275
Size: 53 83 186 936 2710 382 635 1451
Fragments arranged by size:
2710 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4869 5267 5541 5688 6099 4869
Size: 398 274 147 411 5206
Fragments arranged by size:
5206 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6211
Fragments arranged by size:
6211 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2112 3222 3282 3743 1186
Size: 926 1110 60 461 3879
Fragments arranged by size:
3879 1110 926 461 60
BseYI C'CCAG_C
Cuts at: 5639 6135 5639
Size: 496 5940
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5863
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3886 4647 5675 1540
Size: 275 186 1885 761 1028 2301
Fragments arranged by size:
2301 1885 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4497 4582 5653 4497
Size: 85 1071 5280
Fragments arranged by size:
5280 1071 85
BsmI GAATG_Cn'
Cuts at: 3572 3947 4040 3572
Size: 375 93 5968
Fragments arranged by size:
5968 375 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3607 3755 4497 4582 5653 1391
Size: 399 1817 148 742 85 1071 2174
Fragments arranged by size:
2174 1817 1071 742 399 148 85
BspEI T'CCGG_A
Cuts at: 6099 6099
Size: 6436
BspHI T'CATG_A
Cuts at: 4325 6055 4325
Size: 1730 4706
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3791 844
Size: 2947 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4323 1394
Size: 429 2500 3507
Fragments arranged by size:
3507 2500 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4931 5105 65
Size: 1183 3683 174 1396
Fragments arranged by size:
3683 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5080 5267 1700
Size: 110 3270 187 2869
Fragments arranged by size:
3270 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6436
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6436
BssSI C'ACGA_G
Cuts at: 4496 5508 4496
Size: 1012 5424
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2818 794
Size: 2024 4412
BstBI TT'CG_AA
Cuts at: 2983 5240 2983
Size: 2257 4179
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6436
BstYI r'GATC_y
Cuts at: 1972 3413 3766 4133 4518 4535 5987 6073 6085
Size: 1441 353 367 385 17 1452 86 12
Cuts at: 6085 6431 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1441 385 367 353 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 3110 3110
Size: 6436
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5884
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5697
Fragments arranged by size:
5697 683 56
BtsI GCAGTG_nn'
Cuts at: 2777 3566 3952 4746 4766 2777
Size: 789 386 794 20 4447
Fragments arranged by size:
4447 794 789 386 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6436
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6401
DraI TTT'AAA
Cuts at: 3145 3740 3145
Size: 595 5841
DraIII CAC_nnn'GTG
Cuts at: 2053 2053
Size: 6436
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2192 5443 809
Size: 507 876 3251 1802
Fragments arranged by size:
3251 1802 876 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3883 4772 6401
Size: 54 1425 50 275 18 2053 889 1629
Cuts at: 6401 8
Size: 43
Fragments arranged by size:
2053 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3883 1537
Size: 275 2071 4090
Fragments arranged by size:
4090 2071 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2224 2595 3173 3209 3399 3720 4366 1353
Size: 871 371 578 36 190 321 646 3423
Fragments arranged by size:
3423 871 646 578 371 321 190 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5023 5397 5543 387
Size: 1064 138 3434 374 146 1280
Fragments arranged by size:
3434 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2060 2319 2421 3390 4499 5096 5883
Size: 393 428 259 102 969 1109 597 787
Cuts at: 5883 6151 1239
Size: 268 1524
Fragments arranged by size:
1524 1109 969 787 597 428 393 268
259 102
EcoRI G'AATT_C
Cuts at: 3061 3745 4123 3061
Size: 684 378 5374
Fragments arranged by size:
5374 684 378
FspI TGC'GCA
Cuts at: 4942 4942
Size: 6436
HaeII r_GCGC'y
Cuts at: 1678 5583 1678
Size: 3905 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2626 2658 5077 5109 5151
Size: 32 135 32 1054 32 2419 32 42
Cuts at: 5151 5183 1373
Size: 32 2626
Fragments arranged by size:
2626 2419 1054 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2137 2484 3612 3780 4026 669
Size: 988 281 199 347 1128 168 246 3079
Fragments arranged by size:
3079 1128 988 347 281 246 199 168
HindIII A'AGCT_T
Cuts at: 748 3632 748
Size: 2884 3552
HpaI GTT'AAC
Cuts at: 3612 4026 3612
Size: 414 6022
KpnI G_GTAC'C
Cuts at: 3761 3761
Size: 6436
MfeI C'AATT_G
Cuts at: 4035 4035
Size: 6436
MluI A'CGCG_T
Cuts at: 6312 6312
Size: 6436
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2586 5550 5734 1305
Size: 1281 2964 184 2007
Fragments arranged by size:
2964 2007 1281 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4614
Fragments arranged by size:
4614 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3285 4394 4753 4912 518
Size: 648 2119 1109 359 159 2042
Fragments arranged by size:
2119 2042 1109 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3502 3852 4529 5677 5922 1606
Size: 1896 350 677 1148 245 2120
Fragments arranged by size:
2120 1896 1148 677 350 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5884
NdeI CA'TA_TG
Cuts at: 387 3090 387
Size: 2703 3733
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6436
NotI GC'GGCC_GC
Cuts at: 3883 3883
Size: 6436
NspI r_CATG'y
Cuts at: 1132 1229 3794 5339 1132
Size: 97 2565 1545 2229
Fragments arranged by size:
2565 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6436
PciI A'CATG_T
Cuts at: 5335 5335
Size: 6436
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3187 1108
Size: 2079 4357
PfoI T'CCnGG_A
Cuts at: 3034 3034
Size: 6436
PmeI GTTT'AAAC
Cuts at: 3740 3740
Size: 6436
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4505 4537 6018 6050 4505
Size: 32 1481 32 4891
Fragments arranged by size:
4891 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6436
PsiI TTA'TAA
Cuts at: 3638 4006 3638
Size: 368 6068
PspOMI G'GGCC_C
Cuts at: 3603 3603
Size: 6436
PstI C_TGCA'G
Cuts at: 830 2784 3788 830
Size: 1954 1004 3478
Fragments arranged by size:
3478 1954 1004
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6436
PvuII CAG'CTG
Cuts at: 3502 3502
Size: 6436
SalI G'TCGA_C
Cuts at: 1936 3778 1936
Size: 1842 4594
SapI GCTCTTCn'nnn_
Cuts at: 3209 3209
Size: 6436
SbfI CC_TGCA'GG
Cuts at: 3788 3788
Size: 6436
ScaI AGT'ACT
Cuts at: 4684 6396 4684
Size: 1712 4724
SfcI C'TryA_G
Cuts at: 826 1045 2265 2780 3784 4919 5600 5791 826
Size: 219 1220 515 1004 1135 681 191 1471
Fragments arranged by size:
1471 1220 1135 1004 681 515 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6436
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6436
SmaI CCC'GGG
Cuts at: 1872 3763 1872
Size: 1891 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4540 5441 5703 820
Size: 197 926 2597 901 262 1553
Fragments arranged by size:
2597 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4227 493
Size: 3734 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6436
SphI G_CATG'C
Cuts at: 1132 3794 1132
Size: 2662 3774
SspI AAT'ATT
Cuts at: 5 52 2552 2732 4360 5
Size: 47 2500 180 1628 2081
Fragments arranged by size:
2500 2081 1628 180 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6436
StyI C'CwwG_G
Cuts at: 513 1065 2522 2923 3875 513
Size: 552 1457 401 952 3074
Fragments arranged by size:
3074 1457 952 552 401
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4463 4480 4633 4818 5263 325
Size: 1255 2883 17 153 185 445 1498
Fragments arranged by size:
2883 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4682 6394 96
Size: 274 80 33 51 1443 2705 1712 138
Fragments arranged by size:
2705 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3030 3062 6396 6428 3030
Size: 32 3334 32 3038
Fragments arranged by size:
3334 3038 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6436
XbaI T'CTAG_A
Cuts at: 2083 3772 2083
Size: 1689 4747
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3227 3227
Size: 6436
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6436
XmaI C'CCGG_G
Cuts at: 1870 3761 1870
Size: 1891 4545
XmnI GAAnn'nnTTC
Cuts at: 2979 4565 2979
Size: 1586 4850
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4244 276
Size: 53 83 186 936 2710 2468
Fragments arranged by size:
2710 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI Bsu36I EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI