Restriction map for
pFN21AB6101
(Circular) (Six-base) MAPSORT of: pFN21AB6101.seq Check: 6939 from: 1 to: 6436
pFN21AB6101 6436 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2859 2877 4246 278
Size: 53 83 186 936 1323 18 1369 2468
Fragments arranged by size:
2468 1369 1323 936 186 83 53 18
AccI GT'mk_AC
Cuts at: 1937 2836 3051 3779 1937
Size: 899 215 728 4594
Fragments arranged by size:
4594 899 728 215
Acc65I G'GTAC_C
Cuts at: 3757 3757
Size: 6436
AclI AA'CG_TT
Cuts at: 1563 2092 4563 4936 1563
Size: 529 2471 373 3063
Fragments arranged by size:
3063 2471 529 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3338 4499 5883 6151 1632
Size: 1706 1161 1384 268 1917
Fragments arranged by size:
1917 1706 1384 1161 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6239
AflIII A'CryG_T
Cuts at: 1203 3177 5335 6312 1203
Size: 1974 2158 977 1327
Fragments arranged by size:
2158 1974 1327 977
AgeI A'CCGG_T
Cuts at: 5267 5267
Size: 6436
AhdI GACnn_n'nnGTC
Cuts at: 5165 5165
Size: 6436
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6436
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2499 1184
Size: 1315 5121
AlwNI CAG_nnn'CTG
Cuts at: 3122 4179 5751 3122
Size: 1057 1572 3807
Fragments arranged by size:
3807 1572 1057
ApaI G_GGCC'C
Cuts at: 3405 3405
Size: 6436
ApaLI G'TGCA_C
Cuts at: 4493 5649 4493
Size: 1156 5280
ApoI r'AATT_y
Cuts at: 1493 3745 3971 4123 6232 1493
Size: 2252 226 152 2109 1697
Fragments arranged by size:
2252 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4990 160
Size: 872 3958 1606
Fragments arranged by size:
3958 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6436
AvaI C'yCGr_G
Cuts at: 1870 1943 2860 3672 3761 1870
Size: 73 917 812 89 4545
Fragments arranged by size:
4545 917 812 89 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3166 3199 3166
Size: 33 6403
BamHI G'GATC_C
Cuts at: 3265 3572 3766 4133 3265
Size: 307 194 367 5568
Fragments arranged by size:
5568 367 307 194
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2021 3689 3757 5212 618
Size: 325 217 627 234 1668 68 1455 1842
Fragments arranged by size:
1842 1668 1455 627 325 234 217 68
BanII G_rGCy'C
Cuts at: 1391 2016 2810 3395 3405 3755 1391
Size: 625 794 585 10 350 4072
Fragments arranged by size:
4072 794 625 585 350 10
BbeI G_GCGC'C
Cuts at: 2025 2025
Size: 6436
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5465
BbvCI CC'TCA_GC
Cuts at: 2539 2539
Size: 6436
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2356 2390 3276 3310 3429 3463 3474
Size: 34 419 34 886 34 119 34 11
Cuts at: 3474 3508 4627 4661 1903
Size: 34 1119 34 3678
Fragments arranged by size:
3678 1119 886 419 119 34 34 34
34 34 34 11
BciVI GTATCCnnnnn_n'
Cuts at: 3194 4328 5538 3194
Size: 1134 1210 4092
Fragments arranged by size:
4092 1210 1134
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6436
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2486 5047 136
Size: 107 122 71 631 605 814 2561 1525
Fragments arranged by size:
2561 1525 814 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3315 3372 6431 3315
Size: 57 3059 3320
Fragments arranged by size:
3320 3059 57
BlpI GC'TnA_GC
Cuts at: 2469 3853 2469
Size: 1384 5052
Bme1580I G_kGCm'C
Cuts at: 1790 3405 4497 5653 1790
Size: 1615 1092 1156 2573
Fragments arranged by size:
2573 1615 1156 1092
BmgBI CAC'GTC
Cuts at: 1318 2428 1318
Size: 1110 5326
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3209 3408 4167
Size: 416 53 83 399 24 1792 199 759
Cuts at: 4167 5125 442
Size: 958 1753
Fragments arranged by size:
1792 1753 958 759 416 399 199 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2747 1056
Size: 1691 4745
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2480 2480
Size: 6436
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2189 2300 2778 3340 5096 1239
Size: 950 111 478 562 1756 2579
Fragments arranged by size:
2579 1756 950 562 478 111
Bpu10I CC'TnA_GC
Cuts at: 2539 2539
Size: 6436
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4561 5426 5724 4561
Size: 865 298 5273
Fragments arranged by size:
5273 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5099 882
Size: 617 3600 2219
Fragments arranged by size:
3600 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4227 493
Size: 711 3023 2702
Fragments arranged by size:
3023 2702 711
BsaBI GATnn'nnATC
Cuts at: 2351 6430 2351
Size: 4079 2357
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2022 2412 2856 2874
Size: 53 83 186 936 489 390 444 18
Cuts at: 2874 3489 4243 4625 5260 275
Size: 615 754 382 635 1451
Fragments arranged by size:
1451 936 754 635 615 489 444 390
382 186 83 53 18
BsaWI w'CCGG_w
Cuts at: 2750 4869 5267 5541 5688 6099 2750
Size: 2119 398 274 147 411 3087
Fragments arranged by size:
3087 2119 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2095 2125 2792 2822 3407
Size: 30 165 30 692 30 667 30 585
Cuts at: 3407 3437 3669 3699 1178
Size: 30 232 30 3915
Fragments arranged by size:
3915 692 667 585 232 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3690 1186
Size: 2504 3932
BseYI C'CCAG_C
Cuts at: 2814 5639 6135 2814
Size: 2825 496 3115
Fragments arranged by size:
3115 2825 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2453 3214 3478 1163
Size: 573 717 761 264 4121
Fragments arranged by size:
4121 761 717 573 264
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2298 2383 3886 4647 5675 1540
Size: 275 186 297 85 1503 761 1028 2301
Fragments arranged by size:
2301 1503 1028 761 297 275 186 85
BsiHKAI G_wGCw'C
Cuts at: 2016 2611 4497 4582 5653 2016
Size: 595 1886 85 1071 2799
Fragments arranged by size:
2799 1886 1071 595 85
BsmI GAATG_Cn'
Cuts at: 2450 3947 4040 2450
Size: 1497 93 4846
Fragments arranged by size:
4846 1497 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2016 2611 2810 3395 3405 3755 4497
Size: 399 226 595 199 585 10 350 742
Cuts at: 4497 4582 5653 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 595 585 399 350 226
199 85 10
BspEI T'CCGG_A
Cuts at: 2750 6099 2750
Size: 3349 3087
BspHI T'CATG_A
Cuts at: 4325 6055 4325
Size: 1730 4706
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3791 844
Size: 2947 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4323 1394
Size: 429 2500 3507
Fragments arranged by size:
3507 2500 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4931 5105 65
Size: 1183 3683 174 1396
Fragments arranged by size:
3683 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2293 2543 5080 5267 1700
Size: 110 483 250 2537 187 2869
Fragments arranged by size:
2869 2537 483 250 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6436
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6436
BssSI C'ACGA_G
Cuts at: 2819 4496 5508 2819
Size: 1677 1012 3747
Fragments arranged by size:
3747 1677 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6436
BstBI TT'CG_AA
Cuts at: 5240 5240
Size: 6436
BstEII G'GTnAC_C
Cuts at: 1184 2960 1184
Size: 1776 4660
BstYI r'GATC_y
Cuts at: 1972 2753 3265 3315 3372 3572 3724 3766 4133
Size: 781 512 50 57 200 152 42 367
Cuts at: 4133 4518 4535 5987 6073 6085 6431 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 781 512 385 367 346 200
152 86 57 50 42 17 12
BstZ17I GTA'TAC
Cuts at: 2837 2837
Size: 6436
Bsu36I CC'TnA_GG
Cuts at: 2977 2977
Size: 6436
BtgI C'CryG_G
Cuts at: 513 1065 2508 2583 513
Size: 552 1443 75 4366
Fragments arranged by size:
4366 1443 552 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2121 487
Size: 683 56 895 4802
Fragments arranged by size:
4802 895 683 56
BtsI GCAGTG_nn'
Cuts at: 3952 4746 4766 3952
Size: 794 20 5622
Fragments arranged by size:
5622 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6436
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6401
DraI TTT'AAA
Cuts at: 3740 3740
Size: 6436
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3215 3587 5443 809
Size: 507 1899 372 1856 1802
Fragments arranged by size:
1899 1856 1802 507 372
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2295 2380 2505
Size: 54 1425 50 275 18 465 85 125
Cuts at: 2505 2967 3564 3883 4772 6401 8
Size: 462 597 319 889 1629 43
Fragments arranged by size:
1629 1425 889 597 465 462 319 275
125 85 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2295 2380 3883 1537
Size: 275 483 85 1503 4090
Fragments arranged by size:
4090 1503 483 275 85
EarI CTCTTCn'nnn_
Cuts at: 1353 2032 2050 2592 4366 1353
Size: 679 18 542 1774 3423
Fragments arranged by size:
3423 1774 679 542 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5023 5397 5543 387
Size: 1064 138 3434 374 146 1280
Fragments arranged by size:
3434 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2014 2014
Size: 6436
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2189 2300 2778 3338 3340 4499 5096
Size: 393 557 111 478 560 2 1159 597
Cuts at: 5096 5883 6151 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1159 787 597 560 557 478 393
268 111 2
EcoO109I rG'GnC_Cy
Cuts at: 2323 2993 2323
Size: 670 5766
EcoRI G'AATT_C
Cuts at: 3745 4123 3745
Size: 378 6058
FspI TGC'GCA
Cuts at: 4942 4942
Size: 6436
HaeII r_GCGC'y
Cuts at: 1678 2025 5583 1678
Size: 347 3558 2531
Fragments arranged by size:
3558 2531 347
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2384 2416 2651 2683 2864
Size: 32 135 32 812 32 235 32 181
Cuts at: 2864 2896 3601 3633 5077 5109 5151 5183 1373
Size: 32 705 32 1444 32 42 32 2626
Fragments arranged by size:
2626 1444 812 705 235 181 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3250 3780 4026 669
Size: 988 281 1312 530 246 3079
Fragments arranged by size:
3079 1312 988 530 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6436
HpaI GTT'AAC
Cuts at: 4026 4026
Size: 6436
KasI G'GCGC_C
Cuts at: 2021 2021
Size: 6436
KpnI G_GTAC'C
Cuts at: 3761 3761
Size: 6436
MfeI C'AATT_G
Cuts at: 4035 4035
Size: 6436
MluI A'CGCG_T
Cuts at: 6312 6312
Size: 6436
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2292 3450 3726 5550 5734 1305
Size: 987 1158 276 1824 184 2007
Fragments arranged by size:
2007 1824 1158 987 276 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2969 3566 10
Size: 54 1425 343 1137 597 2880
Fragments arranged by size:
2880 1425 1137 597 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3176 4394 4753 4912 518
Size: 648 2010 1218 359 159 2042
Fragments arranged by size:
2042 2010 1218 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3852 4529 5677 5922 1606
Size: 2246 677 1148 245 2120
Fragments arranged by size:
2246 2120 1148 677 245
NaeI GCC'GGC
Cuts at: 2545 2545
Size: 6436
NarI GG'CG_CC
Cuts at: 2022 2022
Size: 6436
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5884
NdeI CA'TA_TG
Cuts at: 387 2926 387
Size: 2539 3897
NgoMIV G'CCGG_C
Cuts at: 2543 2543
Size: 6436
NheI G'CTAG_C
Cuts at: 1052 2743 1052
Size: 1691 4745
NotI GC'GGCC_GC
Cuts at: 3883 3883
Size: 6436
NspI r_CATG'y
Cuts at: 1132 1229 3181 3794 5339 1132
Size: 97 1952 613 1545 2229
Fragments arranged by size:
2229 1952 1545 613 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6436
PciI A'CATG_T
Cuts at: 3177 5335 3177
Size: 2158 4278
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3261 3387 1108
Size: 2153 126 4157
Fragments arranged by size:
4157 2153 126
PfoI T'CCnGG_A
Cuts at: 2205 2205
Size: 6436
PmeI GTTT'AAAC
Cuts at: 3740 3740
Size: 6436
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4505 4537 6018 6050 4505
Size: 32 1481 32 4891
Fragments arranged by size:
4891 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6436
PsiI TTA'TAA
Cuts at: 4006 4006
Size: 6436
PspOMI G'GGCC_C
Cuts at: 3401 3401
Size: 6436
PstI C_TGCA'G
Cuts at: 830 2053 2331 3788 830
Size: 1223 278 1457 3478
Fragments arranged by size:
3478 1457 1223 278
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6436
SacI G_AGCT'C
Cuts at: 2016 2016
Size: 6436
SalI G'TCGA_C
Cuts at: 1936 3778 1936
Size: 1842 4594
SbfI CC_TGCA'GG
Cuts at: 2053 3788 2053
Size: 1735 4701
ScaI AGT'ACT
Cuts at: 3560 4684 6396 3560
Size: 1124 1712 3600
Fragments arranged by size:
3600 1712 1124
SfcI C'TryA_G
Cuts at: 826 1045 2049 2327 3166 3529 3784 4919 5600
Size: 219 1004 278 839 363 255 1135 681
Cuts at: 5600 5791 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1004 839 681 363 278 255
219 191
SfoI GGC'GCC
Cuts at: 2023 2023
Size: 6436
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6436
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6436
SmaI CCC'GGG
Cuts at: 1872 3763 1872
Size: 1891 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4540 5441 5703 820
Size: 197 926 2597 901 262 1553
Fragments arranged by size:
2597 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4227 493
Size: 3734 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6436
SphI G_CATG'C
Cuts at: 1132 3794 1132
Size: 2662 3774
SspI AAT'ATT
Cuts at: 5 52 4360 5
Size: 47 4308 2081
Fragments arranged by size:
4308 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6436
StyI C'CwwG_G
Cuts at: 513 1065 3875 513
Size: 552 2810 3074
Fragments arranged by size:
3074 2810 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2024 2828 3512 3677 4463 4480 4633
Size: 1255 444 804 684 165 786 17 153
Cuts at: 4633 4818 5263 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 804 786 684 445 444 185
165 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3558 4682 6394
Size: 274 80 33 51 1443 1581 1124 1712
Cuts at: 6394 96
Size: 138
Fragments arranged by size:
1712 1581 1443 1124 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2095 2127 3669 3701 6396 6428 2095
Size: 32 1542 32 2695 32 2103
Fragments arranged by size:
2695 2103 1542 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6436
XbaI T'CTAG_A
Cuts at: 3772 3772
Size: 6436
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3433 3433
Size: 6436
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6436
XmaI C'CCGG_G
Cuts at: 1870 3761 1870
Size: 1891 4545
XmnI GAAnn'nnTTC
Cuts at: 4565 4565
Size: 6436
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2857 2875 4244 276
Size: 53 83 186 936 1323 18 1369 2468
Fragments arranged by size:
2468 1369 1323 936 186 83 53 18
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BfrBI BsiWI BsmBI
BstXI DraIII EcoNI EcoRV FalI FseI FspAI NruI
NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII
SacII SanDI SapI SexAI SfiI SrfI SwaI