Restriction map for
pFN21AB6014
(Circular) (Six-base) MAPSORT of: pFN21AB6014.seq Check: 6518 from: 1 to: 6799
pFN21AB6014 6799 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4609 278
Size: 53 83 186 936 3073 2468
Fragments arranged by size:
3073 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4142 1937
Size: 2205 4594
Acc65I G'GTAC_C
Cuts at: 4120 4120
Size: 6799
AclI AA'CG_TT
Cuts at: 1563 4926 5299 1563
Size: 3363 373 3063
Fragments arranged by size:
3363 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2754 3074 3572 4862 6246 6514 1632
Size: 1122 320 498 1290 1384 268 1917
Fragments arranged by size:
1917 1384 1290 1122 498 320 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6602
AflIII A'CryG_T
Cuts at: 1203 3711 5698 6675 1203
Size: 2508 1987 977 1327
Fragments arranged by size:
2508 1987 1327 977
AgeI A'CCGG_T
Cuts at: 5630 5630
Size: 6799
AhdI GACnn_n'nnGTC
Cuts at: 2986 5528 2986
Size: 2542 4257
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6799
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6799
AlwNI CAG_nnn'CTG
Cuts at: 3910 4542 6114 3910
Size: 632 1572 4595
Fragments arranged by size:
4595 1572 632
ApaI G_GGCC'C
Cuts at: 2314 3854 3921 3928 2314
Size: 1540 67 7 5185
Fragments arranged by size:
5185 1540 67 7
ApaLI G'TGCA_C
Cuts at: 3843 4856 6012 3843
Size: 1013 1156 4630
Fragments arranged by size:
4630 1156 1013
ApoI r'AATT_y
Cuts at: 1493 2473 2926 2951 4108 4334 4486 6595 1493
Size: 980 453 25 1157 226 152 2109 1697
Fragments arranged by size:
2109 1697 1157 980 453 226 152 25
AseI AT'TA_AT
Cuts at: 160 1032 5353 160
Size: 872 4321 1606
Fragments arranged by size:
4321 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6799
AvaI C'yCGr_G
Cuts at: 1870 1943 2038 2721 3920 3959 4124 1870
Size: 73 95 683 1199 39 165 4545
Fragments arranged by size:
4545 1199 683 165 95 73 39
BamHI G'GATC_C
Cuts at: 3168 3568 4129 4496 3168
Size: 400 561 367 5471
Fragments arranged by size:
5471 561 400 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4120 5575 618
Size: 325 217 627 2333 1455 1842
Fragments arranged by size:
2333 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2071 2314 3854 3921 3928 4118 1391
Size: 680 243 1540 67 7 190 4072
Fragments arranged by size:
4072 1540 680 243 190 67 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3062 3378 928
Size: 971 1163 316 4349
Fragments arranged by size:
4349 1163 971 316
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4990 5024 1903
Size: 34 3053 34 3678
Fragments arranged by size:
3678 3053 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4691 5901 4691
Size: 1210 5589
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6799
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2021 2305
Size: 107 122 71 631 605 337 12 284
Cuts at: 2305 3552 5410 136
Size: 1247 1858 1525
Fragments arranged by size:
1858 1525 1247 631 605 337 284 122
107 71 12
BglII A'GATC_T
Cuts at: 6794 6794
Size: 6799
BlpI GC'TnA_GC
Cuts at: 4216 4216
Size: 6799
Bme1580I G_kGCm'C
Cuts at: 1790 2314 3847 3854 3921 3928 4860 6016 1790
Size: 524 1533 7 67 7 932 1156 2573
Fragments arranged by size:
2573 1533 1156 932 524 67 7 7
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6799
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2344 2586 3857
Size: 416 53 83 399 24 927 242 1271
Cuts at: 3857 4530 5488 442
Size: 673 958 1753
Fragments arranged by size:
1753 1271 958 927 673 416 399 242
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2126 1056
Size: 1070 5729
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2487 2771 3005 3887 3958 5459 1239
Size: 1248 284 234 882 71 1501 2579
Fragments arranged by size:
2579 1501 1248 882 284 234 71
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3047 4924 5789 6087 3047
Size: 1877 865 298 3759
Fragments arranged by size:
3759 1877 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3804 5462 882
Size: 617 2305 1658 2219
Fragments arranged by size:
2305 2219 1658 617
BsaAI yAC'GTr
Cuts at: 493 1204 2635 3664 3714 4009 4590 493
Size: 711 1431 1029 50 295 581 2702
Fragments arranged by size:
2702 1431 1029 711 581 295 50
BsaBI GATnn'nnATC
Cuts at: 6793 6793
Size: 6799
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2187 4606 4988 5623
Size: 53 83 186 936 654 2419 382 635
Cuts at: 5623 275
Size: 1451
Fragments arranged by size:
2419 1451 936 654 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2430 3601 5232 5630 5904 6051 6462 2430
Size: 1171 1631 398 274 147 411 2767
Fragments arranged by size:
2767 1631 1171 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2988 3018 1178
Size: 30 165 30 1585 30 4959
Fragments arranged by size:
4959 1585 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3418 3788 1186
Size: 2232 370 4197
Fragments arranged by size:
4197 2232 370
BseYI C'CCAG_C
Cuts at: 2318 3613 3648 6002 6498 2318
Size: 1295 35 2354 496 2619
Fragments arranged by size:
2619 2354 1295 496 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2276 1163
Size: 573 540 5686
Fragments arranged by size:
5686 573 540
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2016 2244 3107 3544 4095 4249
Size: 275 186 15 228 863 437 551 154
Cuts at: 4249 5010 6038 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1028 863 761 551 437 275 228
186 154 15
BsiHKAI G_wGCw'C
Cuts at: 2728 3450 3669 3847 4860 4945 6016 2728
Size: 722 219 178 1013 85 1071 3511
Fragments arranged by size:
3511 1071 1013 722 219 178 85
BsmI GAATG_Cn'
Cuts at: 4310 4403 4310
Size: 93 6706
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2071 2314 2728 3450 3669 3847 3854
Size: 399 281 243 414 722 219 178 7
Cuts at: 3854 3921 3928 4118 4860 4945 6016 1391
Size: 67 7 190 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 722 414 399 281 243
219 190 178 85 67 7 7
BspEI T'CCGG_A
Cuts at: 3601 6462 3601
Size: 2861 3938
BspHI T'CATG_A
Cuts at: 4688 6418 4688
Size: 1730 5069
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2065 4154 844
Size: 1221 2089 3489
Fragments arranged by size:
3489 2089 1221
BsrBI CCG'CTC
Cuts at: 1394 1823 2051 4686 1394
Size: 429 228 2635 3507
Fragments arranged by size:
3507 2635 429 228
BsrDI GCAATG_nn'
Cuts at: 65 1248 2867 5294 5468 65
Size: 1183 1619 2427 174 1396
Fragments arranged by size:
2427 1619 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2553 5443 5630 1700
Size: 110 743 2890 187 2869
Fragments arranged by size:
2890 2869 743 187 110
BsrGI T'GTAC_A
Cuts at: 96 2970 96
Size: 2874 3925
BssHII G'CGCG_C
Cuts at: 1925 2030 1925
Size: 105 6694
BssSI C'ACGA_G
Cuts at: 2252 4859 5871 2252
Size: 2607 1012 3180
Fragments arranged by size:
3180 2607 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6799
BstBI TT'CG_AA
Cuts at: 5603 5603
Size: 6799
BstEII G'GTnAC_C
Cuts at: 1184 2716 3284 1184
Size: 1532 568 4699
Fragments arranged by size:
4699 1532 568
BstYI r'GATC_y
Cuts at: 1972 3168 3568 4129 4496 4881 4898 6350 6436
Size: 1196 400 561 367 385 17 1452 86
Cuts at: 6436 6448 6794 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1196 561 400 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2087 2105 2620 3474 3805 4095
Size: 552 939 83 18 515 854 331 290
Cuts at: 4095 513
Size: 3217
Fragments arranged by size:
3217 939 854 552 515 331 290 83
18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6060
Fragments arranged by size:
6060 683 56
BtsI GCAGTG_nn'
Cuts at: 2281 4315 5109 5129 2281
Size: 2034 794 20 3951
Fragments arranged by size:
3951 2034 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6799
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6764
DraI TTT'AAA
Cuts at: 2440 2479 4103 2440
Size: 39 1624 5136
Fragments arranged by size:
5136 1624 39
DraIII CAC_nnn'GTG
Cuts at: 3842 3842
Size: 6799
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5806 809
Size: 507 4490 1802
Fragments arranged by size:
4490 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2013 2241 2278
Size: 54 1425 50 275 18 183 228 37
Cuts at: 2278 3039 3084 3104 3147 3541 3642 3691 3756
Size: 761 45 20 43 394 101 49 65
Cuts at: 3756 4092 4246 5135 6764 8
Size: 336 154 889 1629 43
Fragments arranged by size:
1629 1425 889 761 394 336 275 228
183 154 101 65 54 50 49 45
43 43 37 20 18
EagI C'GGCC_G
Cuts at: 1537 1812 2013 2241 3104 3541 4092 4246 1537
Size: 275 201 228 863 437 551 154 4090
Fragments arranged by size:
4090 863 551 437 275 228 201 154
EarI CTCTTCn'nnn_
Cuts at: 1353 2370 3765 4729 1353
Size: 1017 1395 964 3423
Fragments arranged by size:
3423 1395 1017 964
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5386 5760 5906 387
Size: 1064 138 3797 374 146 1280
Fragments arranged by size:
3797 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2487 2754 2771 3005 3074 3572 3887
Size: 393 855 267 17 234 69 498 315
Cuts at: 3887 3958 4862 5459 6246 6514 1239
Size: 71 904 597 787 268 1524
Fragments arranged by size:
1524 904 855 787 597 498 393 315
268 267 234 71 69 17
EcoO109I rG'GnC_Cy
Cuts at: 2054 2311 2483 3637 3851 3924 2054
Size: 257 172 1154 214 73 4929
Fragments arranged by size:
4929 1154 257 214 172 73
EcoRI G'AATT_C
Cuts at: 4108 4486 4108
Size: 378 6421
FspI TGC'GCA
Cuts at: 5305 5305
Size: 6799
HaeII r_GCGC'y
Cuts at: 1678 2077 5946 1678
Size: 399 3869 2531
Fragments arranged by size:
3869 2531 399
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2972 3004 3553 3585 3817
Size: 32 135 32 1400 32 549 32 232
Cuts at: 3817 3849 3865 3897 5440 5472 5514 5546 1373
Size: 32 16 32 1543 32 42 32 2626
Fragments arranged by size:
2626 1543 1400 549 232 135 42 32
32 32 32 32 32 32 32 16
HincII GTy'rAC
Cuts at: 669 1657 1938 3199 3967 4143 4389 669
Size: 988 281 1261 768 176 246 3079
Fragments arranged by size:
3079 1261 988 768 281 246 176
HindIII A'AGCT_T
Cuts at: 748 3584 748
Size: 2836 3963
HpaI GTT'AAC
Cuts at: 4389 4389
Size: 6799
KpnI G_GTAC'C
Cuts at: 4124 4124
Size: 6799
MfeI C'AATT_G
Cuts at: 4398 4398
Size: 6799
MluI A'CGCG_T
Cuts at: 6675 6675
Size: 6799
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3105 3232 5913 6097 1305
Size: 1800 127 2681 184 2007
Fragments arranged by size:
2681 2007 1800 184 127
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2280 3086 3149 3644 3758
Size: 54 1425 343 448 806 63 495 114
Cuts at: 3758 10
Size: 3051
Fragments arranged by size:
3051 1425 806 495 448 343 114 63
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2714 3345 3561 4034 4757 5116 5275
Size: 648 1548 631 216 473 723 359 159
Cuts at: 5275 518
Size: 2042
Fragments arranged by size:
2042 1548 723 648 631 473 359 216
159
MspA1I CmG'CkG
Cuts at: 1606 2089 2107 2208 3648 4215 4892 6040 6285
Size: 483 18 101 1440 567 677 1148 245
Cuts at: 6285 1606
Size: 2120
Fragments arranged by size:
2120 1440 1148 677 567 483 245 101
18
NaeI GCC'GGC
Cuts at: 2555 2555
Size: 6799
NcoI C'CATG_G
Cuts at: 513 1065 2004 3474 3805 513
Size: 552 939 1470 331 3507
Fragments arranged by size:
3507 1470 939 552 331
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6799
NgoMIV G'CCGG_C
Cuts at: 2553 2553
Size: 6799
NheI G'CTAG_C
Cuts at: 1052 2122 1052
Size: 1070 5729
NotI GC'GGCC_GC
Cuts at: 4246 4246
Size: 6799
NspI r_CATG'y
Cuts at: 1132 1229 4157 5702 1132
Size: 97 2928 1545 2229
Fragments arranged by size:
2928 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6799
PciI A'CATG_T
Cuts at: 5698 5698
Size: 6799
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2635 3510 3910 4068 1108
Size: 1527 875 400 158 3839
Fragments arranged by size:
3839 1527 875 400 158
PmeI GTTT'AAAC
Cuts at: 4103 4103
Size: 6799
PmlI CAC'GTG
Cuts at: 3664 3664
Size: 6799
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4868 4900 6381 6413 4868
Size: 32 1481 32 5254
Fragments arranged by size:
5254 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2054 2483 2054
Size: 429 6370
PshAI GACnn'nnGTC
Cuts at: 1613 3987 1613
Size: 2374 4425
PsiI TTA'TAA
Cuts at: 4369 4369
Size: 6799
PspOMI G'GGCC_C
Cuts at: 2310 3850 3917 3924 2310
Size: 1540 67 7 5185
Fragments arranged by size:
5185 1540 67 7
PstI C_TGCA'G
Cuts at: 830 2535 3138 3396 4151 830
Size: 1705 603 258 755 3478
Fragments arranged by size:
3478 1705 755 603 258
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6799
PvuII CAG'CTG
Cuts at: 3648 3648
Size: 6799
SacII CC_GC'GG
Cuts at: 2090 2108 2090
Size: 18 6781
SalI G'TCGA_C
Cuts at: 1936 4141 1936
Size: 2205 4594
SbfI CC_TGCA'GG
Cuts at: 4151 4151
Size: 6799
ScaI AGT'ACT
Cuts at: 5047 6759 5047
Size: 1712 5087
SexAI A'CCwGG_T
Cuts at: 3192 3838 3192
Size: 646 6153
SfcI C'TryA_G
Cuts at: 826 1045 2531 3134 3392 4147 5282 5963 6154
Size: 219 1486 603 258 755 1135 681 191
Cuts at: 6154 826
Size: 1471
Fragments arranged by size:
1486 1471 1135 755 681 603 258 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6799
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6799
SmaI CCC'GGG
Cuts at: 1872 2040 3922 4126 1872
Size: 168 1882 204 4545
Fragments arranged by size:
4545 1882 204 168
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3062 4903 5804 6066 820
Size: 197 926 1119 1841 901 262 1553
Fragments arranged by size:
1841 1553 1119 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4590 493
Size: 4097 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6799
SphI G_CATG'C
Cuts at: 1132 4157 1132
Size: 3025 3774
SspI AAT'ATT
Cuts at: 5 52 3225 4723 5
Size: 47 3173 1498 2081
Fragments arranged by size:
3173 2081 1498 47
StuI AGG'CCT
Cuts at: 1517 3859 1517
Size: 2342 4457
StyI C'CwwG_G
Cuts at: 513 1065 2004 2668 3474 3805 4238 513
Size: 552 939 664 806 331 433 3074
Fragments arranged by size:
3074 939 806 664 552 433 331
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2185 2505 3357 3417 3828 3836 4826
Size: 1255 605 320 852 60 411 8 990
Cuts at: 4826 4843 4996 5181 5626 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1255 990 852 605 445 411 320
185 153 60 17 8
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2970 4038 5045
Size: 274 80 33 51 1443 993 1068 1007
Cuts at: 5045 6757 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1068 1007 993 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2177 2209 2664 2696 6759 6791 2177
Size: 32 455 32 4063 32 2185
Fragments arranged by size:
4063 2185 455 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3194 1316
Size: 1878 4921
XbaI T'CTAG_A
Cuts at: 4135 4135
Size: 6799
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3157 3588 3911 3157
Size: 431 323 6045
Fragments arranged by size:
6045 431 323
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6799
XmaI C'CCGG_G
Cuts at: 1870 2038 3920 4124 1870
Size: 168 1882 204 4545
Fragments arranged by size:
4545 1882 204 168
XmnI GAAnn'nnTTC
Cuts at: 3308 4928 3308
Size: 1620 5179
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4607 276
Size: 53 83 186 936 3073 2468
Fragments arranged by size:
3073 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PfoI PspXI PsrI RsrII SacI
SanDI SapI SfiI SfoI SrfI SwaI