Restriction map for
pFN21AB6013
(Circular) (Six-base) MAPSORT of: pFN21AB6013.seq Check: 6391 from: 1 to: 6433
pFN21AB6013 6433 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2038 2195 2870 2038
Size: 157 675 5601
Fragments arranged by size:
5601 675 157
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3708 4243 278
Size: 53 83 186 936 2172 535 2468
Fragments arranged by size:
2468 2172 936 535 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3776 1937
Size: 1839 4594
Acc65I G'GTAC_C
Cuts at: 3754 3754
Size: 6433
AclI AA'CG_TT
Cuts at: 1563 4560 4933 1563
Size: 2997 373 3063
Fragments arranged by size:
3063 2997 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3011 4496 5880 6148 1632
Size: 1379 1485 1384 268 1917
Fragments arranged by size:
1917 1485 1384 1379 268
AfeI AGC'GCT
Cuts at: 2058 3335 2058
Size: 1277 5156
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6236
AflIII A'CryG_T
Cuts at: 1203 2333 5332 6309 1203
Size: 1130 2999 977 1327
Fragments arranged by size:
2999 1327 1130 977
AgeI A'CCGG_T
Cuts at: 5264 5264
Size: 6433
AhdI GACnn_n'nnGTC
Cuts at: 5162 5162
Size: 6433
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6433
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3060 3568 3635 1184
Size: 1876 508 67 3982
Fragments arranged by size:
3982 1876 508 67
AlwNI CAG_nnn'CTG
Cuts at: 3378 4176 5748 3378
Size: 798 1572 4063
Fragments arranged by size:
4063 1572 798
ApaI G_GGCC'C
Cuts at: 2014 2309 3186 2014
Size: 295 877 5261
Fragments arranged by size:
5261 877 295
ApaLI G'TGCA_C
Cuts at: 2654 4490 5646 2654
Size: 1836 1156 3441
Fragments arranged by size:
3441 1836 1156
ApoI r'AATT_y
Cuts at: 1493 3742 3968 4120 6229 1493
Size: 2249 226 152 2109 1697
Fragments arranged by size:
2249 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4987 160
Size: 872 3955 1606
Fragments arranged by size:
3955 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6433
AvaI C'yCGr_G
Cuts at: 1870 1943 2081 3436 3758 1870
Size: 73 138 1355 322 4545
Fragments arranged by size:
4545 1355 322 138 73
BamHI G'GATC_C
Cuts at: 3763 4130 3763
Size: 367 6066
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2086 3754 5209 618
Size: 325 217 627 299 1668 1455 1842
Fragments arranged by size:
1842 1668 1455 627 325 299 217
BanII G_rGCy'C
Cuts at: 1391 2014 2309 2366 2430 3186 3752 1391
Size: 623 295 57 64 756 566 4072
Fragments arranged by size:
4072 756 623 566 295 64 57
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3701 928
Size: 971 1802 3660
Fragments arranged by size:
3660 1802 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4624 4658 1903
Size: 34 2687 34 3678
Fragments arranged by size:
3678 2687 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4325 5535 4325
Size: 1210 5223
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6433
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2313 2961
Size: 107 122 71 631 605 337 304 648
Cuts at: 2961 3228 5044 136
Size: 267 1816 1525
Fragments arranged by size:
1816 1525 648 631 605 337 304 267
122 107 71
BglII A'GATC_T
Cuts at: 6428 6428
Size: 6433
BlpI GC'TnA_GC
Cuts at: 3850 3850
Size: 6433
Bme1580I G_kGCm'C
Cuts at: 1790 2014 2091 2309 2658 3186 3314 3658 4494
Size: 224 77 218 349 528 128 344 836
Cuts at: 4494 5650 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 836 528 349 344 224 218
128 77
BmgBI CAC'GTC
Cuts at: 1318 2290 1318
Size: 972 5461
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2303 3550 4164
Size: 416 53 83 399 24 886 1247 614
Cuts at: 4164 5122 442
Size: 958 1753
Fragments arranged by size:
1753 1247 958 886 614 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6433
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2512 3451 2512
Size: 939 5494
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5093 1239
Size: 3854 2579
Bpu10I CC'TnA_GC
Cuts at: 2613 2613
Size: 6433
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3476 3695 4558 5423 5721 3476
Size: 219 863 865 298 4188
Fragments arranged by size:
4188 865 863 298 219
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2040 2216 2567 2600 3104 5096 882
Size: 617 541 176 351 33 504 1992 2219
Fragments arranged by size:
2219 1992 617 541 504 351 176 33
BsaAI yAC'GTr
Cuts at: 493 1204 3535 4224 493
Size: 711 2331 689 2702
Fragments arranged by size:
2702 2331 711 689
BsaBI GATnn'nnATC
Cuts at: 6427 6427
Size: 6433
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3705 4240 4622 5257
Size: 53 83 186 936 2172 535 382 635
Cuts at: 5257 275
Size: 1451
Fragments arranged by size:
2172 1451 936 635 535 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4866 5264 5538 5685 6096 4866
Size: 398 274 147 411 5203
Fragments arranged by size:
5203 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2726 2756 1178
Size: 30 165 30 1323 30 4855
Fragments arranged by size:
4855 1323 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3317 3440 1186
Size: 2131 123 4179
Fragments arranged by size:
4179 2131 123
BseYI C'CCAG_C
Cuts at: 2040 2429 2814 2835 2849 2882 3207 5636 6132
Size: 389 385 21 14 33 325 2429 496
Cuts at: 6132 2040
Size: 2341
Fragments arranged by size:
2429 2341 496 389 385 325 33 21
14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2848 3181 1163
Size: 573 1112 333 4415
Fragments arranged by size:
4415 1112 573 333
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2982 3883 4644 5672 1540
Size: 275 186 981 901 761 1028 2301
Fragments arranged by size:
2301 1028 981 901 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2186 2658 4494 4579 5650 2186
Size: 472 1836 85 1071 2969
Fragments arranged by size:
2969 1836 1071 472 85
BsmI GAATG_Cn'
Cuts at: 3944 4037 3944
Size: 93 6340
BsmBI CGTCTCn'nnnn_
Cuts at: 2198 2198
Size: 6433
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2014 2091 2186 2309 2366 2430 2658
Size: 399 224 77 95 123 57 64 228
Cuts at: 2658 3186 3314 3658 3752 4494 4579 5650 1391
Size: 528 128 344 94 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 528 399 344 228 224
128 123 95 94 85 77 64 57
BspEI T'CCGG_A
Cuts at: 6096 6096
Size: 6433
BspHI T'CATG_A
Cuts at: 4322 6052 4322
Size: 1730 4703
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2038 2195 2447 2534 2870 3788 844
Size: 1194 157 252 87 336 918 3489
Fragments arranged by size:
3489 1194 918 336 252 157 87
BsrBI CCG'CTC
Cuts at: 1394 1823 3144 3583 4320 1394
Size: 429 1321 439 737 3507
Fragments arranged by size:
3507 1321 737 439 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2808 3274 4928 5102 65
Size: 1183 1560 466 1654 174 1396
Fragments arranged by size:
1654 1560 1396 1183 466 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2025 2132 2709 5077 5264 1700
Size: 110 215 107 577 2368 187 2869
Fragments arranged by size:
2869 2368 577 215 187 110 107
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6433
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6433
BssSI C'ACGA_G
Cuts at: 4493 5505 4493
Size: 1012 5421
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3653 794
Size: 2859 3574
BstBI TT'CG_AA
Cuts at: 5237 5237
Size: 6433
BstEII G'GTnAC_C
Cuts at: 1184 2665 2800 1184
Size: 1481 135 4817
Fragments arranged by size:
4817 1481 135
BstXI CCAn_nnnn'nTGG
Cuts at: 2786 2786
Size: 6433
BstYI r'GATC_y
Cuts at: 1972 2853 3763 4130 4515 4532 5984 6070 6082
Size: 881 910 367 385 17 1452 86 12
Cuts at: 6082 6428 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 910 881 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2891 2891
Size: 6433
BtgI C'CryG_G
Cuts at: 513 1065 2670 513
Size: 552 1605 4276
Fragments arranged by size:
4276 1605 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5694
Fragments arranged by size:
5694 683 56
BtsI GCAGTG_nn'
Cuts at: 3082 3949 4743 4763 3082
Size: 867 794 20 4752
Fragments arranged by size:
4752 867 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6433
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2233 2268 529
Size: 35 1669 35 4694
Fragments arranged by size:
4694 1669 35 35
DraI TTT'AAA
Cuts at: 3737 3737
Size: 6433
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5440 809
Size: 507 4124 1802
Fragments arranged by size:
4124 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2386 2514 2979
Size: 54 1425 50 275 18 556 128 465
Cuts at: 2979 3079 3153 3615 3880 4769 6398 8
Size: 100 74 462 265 889 1629 43
Fragments arranged by size:
1629 1425 889 556 465 462 275 265
128 100 74 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2979 3880 1537
Size: 275 1167 901 4090
Fragments arranged by size:
4090 1167 901 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3015 4363 1353
Size: 1662 1348 3423
Fragments arranged by size:
3423 1662 1348
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5020 5394 5540 387
Size: 1064 138 3431 374 146 1280
Fragments arranged by size:
3431 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3011 4496 5093 5880 6148 1239
Size: 393 1379 1485 597 787 268 1524
Fragments arranged by size:
1524 1485 1379 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3319 3503 3319
Size: 184 6249
EcoO109I rG'GnC_Cy
Cuts at: 2011 2305 2887 3182 3183 3466 2011
Size: 294 582 295 1 283 4978
Fragments arranged by size:
4978 582 295 294 283 1
EcoRI G'AATT_C
Cuts at: 3742 4120 3742
Size: 378 6055
FseI GG_CCGG'CC
Cuts at: 2713 2713
Size: 6433
FspI TGC'GCA
Cuts at: 4939 4939
Size: 6433
HaeII r_GCGC'y
Cuts at: 1678 2060 2108 3337 5580 1678
Size: 382 48 1229 2243 2531
Fragments arranged by size:
2531 2243 1229 382 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5074 5106 5148 5180 1373
Size: 32 135 32 3502 32 42 32 2626
Fragments arranged by size:
3502 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3777 4023 669
Size: 988 281 1839 246 3079
Fragments arranged by size:
3079 1839 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3671 748
Size: 2923 3510
HpaI GTT'AAC
Cuts at: 4023 4023
Size: 6433
KpnI G_GTAC'C
Cuts at: 3758 3758
Size: 6433
MfeI C'AATT_G
Cuts at: 4032 4032
Size: 6433
MluI A'CGCG_T
Cuts at: 6309 6309
Size: 6433
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2724 5547 5731 1305
Size: 1419 2823 184 2007
Fragments arranged by size:
2823 2007 1419 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2388 3617 10
Size: 54 1425 343 556 1229 2826
Fragments arranged by size:
2826 1425 1229 556 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2288 2784 3042 3590 3617 4391 4750
Size: 648 1122 496 258 548 27 774 359
Cuts at: 4750 4909 518
Size: 159 2042
Fragments arranged by size:
2042 1122 774 648 548 496 359 258
159 27
MspA1I CmG'CkG
Cuts at: 1606 2415 2472 2818 2839 2908 3033 3151 3849
Size: 809 57 346 21 69 125 118 698
Cuts at: 3849 4526 5674 5919 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 809 698 677 346 245 125
118 69 57 21
NaeI GCC'GGC
Cuts at: 2134 2711 2134
Size: 577 5856
NcoI C'CATG_G
Cuts at: 513 1065 2670 513
Size: 552 1605 4276
Fragments arranged by size:
4276 1605 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6433
NgoMIV G'CCGG_C
Cuts at: 2132 2709 2132
Size: 577 5856
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6433
NotI GC'GGCC_GC
Cuts at: 3880 3880
Size: 6433
NspI r_CATG'y
Cuts at: 1132 1229 2320 2337 3130 3549 3791 5336 1132
Size: 97 1091 17 793 419 242 1545 2229
Fragments arranged by size:
2229 1545 1091 793 419 242 97 17
PasI CC'CwG_GG
Cuts at: 1348 3321 1348
Size: 1973 4460
PciI A'CATG_T
Cuts at: 2333 5332 2333
Size: 2999 3434
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2166 1108
Size: 1058 5375
PmeI GTTT'AAAC
Cuts at: 3737 3737
Size: 6433
PmlI CAC'GTG
Cuts at: 3535 3535
Size: 6433
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4502 4534 6015 6047 4502
Size: 32 1481 32 4888
Fragments arranged by size:
4888 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2887 3466 2887
Size: 579 5854
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6433
PsiI TTA'TAA
Cuts at: 4003 4003
Size: 6433
PspOMI G'GGCC_C
Cuts at: 2010 2305 3182 2010
Size: 295 877 5261
Fragments arranged by size:
5261 877 295
PstI C_TGCA'G
Cuts at: 830 2192 2358 2844 2938 3180 3785 830
Size: 1362 166 486 94 242 605 3478
Fragments arranged by size:
3478 1362 605 486 242 166 94
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6433
PvuII CAG'CTG
Cuts at: 2818 2839 2818
Size: 21 6412
SalI G'TCGA_C
Cuts at: 1936 3775 1936
Size: 1839 4594
SapI GCTCTTCn'nnn_
Cuts at: 3015 3015
Size: 6433
SbfI CC_TGCA'GG
Cuts at: 2192 3180 3785 2192
Size: 988 605 4840
Fragments arranged by size:
4840 988 605
ScaI AGT'ACT
Cuts at: 4681 6393 4681
Size: 1712 4721
SfcI C'TryA_G
Cuts at: 826 1045 2188 2354 2840 2934 3176 3781 4916
Size: 219 1143 166 486 94 242 605 1135
Cuts at: 4916 5597 5788 826
Size: 681 191 1471
Fragments arranged by size:
1471 1143 1135 681 605 486 242 219
191 166 94
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6433
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6433
SmaI CCC'GGG
Cuts at: 1872 3760 1872
Size: 1888 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3491 3710 4537 5438 5700 820
Size: 197 926 1548 219 827 901 262 1553
Fragments arranged by size:
1553 1548 926 901 827 262 219 197
SnaBI TAC'GTA
Cuts at: 493 4224 493
Size: 3731 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6433
SphI G_CATG'C
Cuts at: 1132 2320 3130 3549 3791 1132
Size: 1188 810 419 242 3774
Fragments arranged by size:
3774 1188 810 419 242
SspI AAT'ATT
Cuts at: 5 52 4357 5
Size: 47 4305 2081
Fragments arranged by size:
4305 2081 47
StuI AGG'CCT
Cuts at: 1517 2492 3122 3296 1517
Size: 975 630 174 4654
Fragments arranged by size:
4654 975 630 174
StyI C'CwwG_G
Cuts at: 513 1065 2015 2322 2670 3872 513
Size: 552 950 307 348 1202 3074
Fragments arranged by size:
3074 1202 950 552 348 307
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2158 2653 2663 3437 4460 4477 4630
Size: 1255 578 495 10 774 1023 17 153
Cuts at: 4630 4815 5260 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1023 774 578 495 445 185
153 17 10
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4679 6391 96
Size: 274 80 33 51 1443 2702 1712 138
Fragments arranged by size:
2702 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2201 2233 3661 3693 6393 6425 2201
Size: 32 1428 32 2700 32 2209
Fragments arranged by size:
2700 2209 1428 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3392 1316
Size: 2076 4357
XbaI T'CTAG_A
Cuts at: 3769 3769
Size: 6433
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2167 2266 2167
Size: 99 6334
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6433
XmaI C'CCGG_G
Cuts at: 1870 3758 1870
Size: 1888 4545
XmnI GAAnn'nnTTC
Cuts at: 4562 4562
Size: 6433
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3706 4241 276
Size: 53 83 186 936 2172 535 2468
Fragments arranged by size:
2468 2172 936 535 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI
BstZ17I DraIII EcoICRI EcoRV FalI FspAI KasI NarI
NruI NsiI PacI PfoI PspXI PsrI RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI