Restriction map for
pFN21AB6010
(Circular) (Six-base) MAPSORT of: pFN21AB6010.seq Check: 1412 from: 1 to: 8398
pFN21AB6010 8398 bp
With 182 enzymes: SgfI *
August 20, 2012 11:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6208 278
Size: 53 83 186 936 4672 2468
Fragments arranged by size:
4672 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4448 5741 1937
Size: 2511 1293 4594
Fragments arranged by size:
4594 2511 1293
Acc65I G'GTAC_C
Cuts at: 5719 5719
Size: 8398
AclI AA'CG_TT
Cuts at: 1563 6525 6898 1563
Size: 4962 373 3063
Fragments arranged by size:
4962 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2472 2810 3298 4381 5304 5654 6461 7845
Size: 840 338 488 1083 923 350 807 1384
Cuts at: 7845 8113 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1083 923 840 807 488 350
338 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8201
AflIII A'CryG_T
Cuts at: 1203 4428 7297 8274 1203
Size: 3225 2869 977 1327
Fragments arranged by size:
3225 2869 1327 977
AgeI A'CCGG_T
Cuts at: 7229 7229
Size: 8398
AhdI GACnn_n'nnGTC
Cuts at: 5173 7127 5173
Size: 1954 6444
AleI CACnn'nnGTG
Cuts at: 1166 4207 1166
Size: 3041 5357
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3576 3849 1184
Size: 2392 273 5733
Fragments arranged by size:
5733 2392 273
AlwNI CAG_nnn'CTG
Cuts at: 6141 7713 6141
Size: 1572 6826
ApaI G_GGCC'C
Cuts at: 2153 2153
Size: 8398
ApaLI G'TGCA_C
Cuts at: 6455 7611 6455
Size: 1156 7242
ApoI r'AATT_y
Cuts at: 1493 2456 2534 2913 3293 3995 4591 4740 4940
Size: 963 78 379 380 702 596 149 200
Cuts at: 4940 4990 5018 5140 5707 5933 6085 8194 1493
Size: 50 28 122 567 226 152 2109 1697
Fragments arranged by size:
2109 1697 963 702 596 567 380 379
226 200 152 149 122 78 50 28
AseI AT'TA_AT
Cuts at: 160 1032 2721 6952 160
Size: 872 1689 4231 1606
Fragments arranged by size:
4231 1689 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8398
AvaI C'yCGr_G
Cuts at: 1870 1943 5723 1870
Size: 73 3780 4545
Fragments arranged by size:
4545 3780 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3870 3903 3870
Size: 33 8365
BamHI G'GATC_C
Cuts at: 5728 6095 5728
Size: 367 8031
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5719 7174 618
Size: 325 217 627 3932 1455 1842
Fragments arranged by size:
3932 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2153 5391 5717 1391
Size: 762 3238 326 4072
Fragments arranged by size:
4072 3238 762 326
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3367 3671 4495 928
Size: 971 1468 304 824 4831
Fragments arranged by size:
4831 1468 971 824 304
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2907 2941 6589 6623 1903
Size: 34 970 34 3648 34 3678
Fragments arranged by size:
3678 3648 970 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2590 6290 7500 2590
Size: 3700 1210 3488
Fragments arranged by size:
3700 3488 1210
BclI T'GATC_A
Cuts at: 1548 4888 1548
Size: 3340 5058
BfrBI ATG'CAT
Cuts at: 3013 4055 3013
Size: 1042 7356
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7009 136
Size: 107 122 71 631 605 5337 1525
Fragments arranged by size:
5337 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8393 8393
Size: 8398
BlpI GC'TnA_GC
Cuts at: 4481 5815 4481
Size: 1334 7064
Bme1580I G_kGCm'C
Cuts at: 1790 2153 6459 7615 1790
Size: 363 4306 1156 2573
Fragments arranged by size:
4306 2573 1156 363
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8398
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3496 6129 7087
Size: 416 53 83 399 24 2079 2633 958
Cuts at: 7087 442
Size: 1753
Fragments arranged by size:
2633 2079 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 5153 1056
Size: 4097 4301
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3314 3314
Size: 8398
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2001 3309 7058 1239
Size: 762 1308 3749 2579
Fragments arranged by size:
3749 2579 1308 762
Bpu10I CC'TnA_GC
Cuts at: 4182 4182
Size: 8398
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6523 7388 7686 6523
Size: 865 298 7235
Fragments arranged by size:
7235 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7061 882
Size: 617 5562 2219
Fragments arranged by size:
5562 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 6189 493
Size: 711 4985 2702
Fragments arranged by size:
4985 2702 711
BsaBI GATnn'nnATC
Cuts at: 2037 4056 5469 8392 2037
Size: 2019 1413 2923 2043
Fragments arranged by size:
2923 2043 2019 1413
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6205 6587 7222 275
Size: 53 83 186 936 4672 382 635 1451
Fragments arranged by size:
4672 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3852 6831 7229 7503 7650 8061 3852
Size: 2979 398 274 147 411 4189
Fragments arranged by size:
4189 2979 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2868 2898 1178
Size: 30 165 30 1465 30 6678
Fragments arranged by size:
6678 1465 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2196 3347 1186
Size: 1010 1151 6237
Fragments arranged by size:
6237 1151 1010
BseYI C'CCAG_C
Cuts at: 2152 7601 8097 2152
Size: 5449 496 2453
Fragments arranged by size:
5449 2453 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4554 4562 5318 1163
Size: 573 2818 8 756 4243
Fragments arranged by size:
4243 2818 756 573 8
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5848 6609 7637 1540
Size: 275 186 3847 761 1028 2301
Fragments arranged by size:
3847 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3565 6459 6544 7615 3565
Size: 2894 85 1071 4348
Fragments arranged by size:
4348 2894 1071 85
BsmI GAATG_Cn'
Cuts at: 2866 5909 6002 2866
Size: 3043 93 5262
Fragments arranged by size:
5262 3043 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2153 3565 5391 5717 6459 6544 7615
Size: 399 363 1412 1826 326 742 85 1071
Cuts at: 7615 1391
Size: 2174
Fragments arranged by size:
2174 1826 1412 1071 742 399 363 326
85
BspEI T'CCGG_A
Cuts at: 3852 8061 3852
Size: 4209 4189
BspHI T'CATG_A
Cuts at: 5079 5418 6287 8017 5079
Size: 339 869 1730 5460
Fragments arranged by size:
5460 1730 869 339
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5753 844
Size: 4909 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 6285 1394
Size: 429 4462 3507
Fragments arranged by size:
4462 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2564 4463 4911 6893 7067 65
Size: 1183 1316 1899 448 1982 174 1396
Fragments arranged by size:
1982 1899 1396 1316 1183 448 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3267 4002 7042 7229 1700
Size: 110 1457 735 3040 187 2869
Fragments arranged by size:
3040 2869 1457 735 187 110
BsrGI T'GTAC_A
Cuts at: 96 2884 96
Size: 2788 5610
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8398
BssSI C'ACGA_G
Cuts at: 6458 7470 6458
Size: 1012 7386
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8398
BstBI TT'CG_AA
Cuts at: 2440 7202 2440
Size: 4762 3636
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8398
BstXI CCAn_nnnn'nTGG
Cuts at: 2234 2234
Size: 8398
BstYI r'GATC_y
Cuts at: 1972 3325 3849 5554 5728 6095 6480 6497 7949
Size: 1353 524 1705 174 367 385 17 1452
Cuts at: 7949 8035 8047 8393 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1705 1452 1353 524 385 367 346
174 86 17 12
BstZ17I GTA'TAC
Cuts at: 4449 4449
Size: 8398
Bsu36I CC'TnA_GG
Cuts at: 5406 5406
Size: 8398
BtgI C'CryG_G
Cuts at: 513 1065 2490 3618 3708 5583 513
Size: 552 1425 1128 90 1875 3328
Fragments arranged by size:
3328 1875 1425 1128 552 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7659
Fragments arranged by size:
7659 683 56
BtsI GCAGTG_nn'
Cuts at: 3219 4086 4202 4214 5330 5914 6708 6728 3219
Size: 867 116 12 1116 584 794 20 4889
Fragments arranged by size:
4889 1116 867 794 584 116 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8398
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8363
DraI TTT'AAA
Cuts at: 2872 2991 4287 4951 4989 5702 2872
Size: 119 1296 664 38 713 5568
Fragments arranged by size:
5568 1296 713 664 119 38
DraIII CAC_nnn'GTG
Cuts at: 3527 3527
Size: 8398
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2636 3042 7405 809
Size: 507 1320 406 4363 1802
Fragments arranged by size:
4363 1802 1320 507 406
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3269 5845 6734
Size: 54 1425 50 275 18 1439 2576 889
Cuts at: 6734 8363 8
Size: 1629 43
Fragments arranged by size:
2576 1629 1439 1425 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5845 1537
Size: 275 4033 4090
Fragments arranged by size:
4090 4033 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2928 3317 3349 3510 3619 3924 4073 5098
Size: 1575 389 32 161 109 305 149 1025
Cuts at: 5098 5499 6328 1353
Size: 401 829 3423
Fragments arranged by size:
3423 1575 1025 829 401 389 305 161
149 109 32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6985 7359 7505 387
Size: 1064 138 5396 374 146 1280
Fragments arranged by size:
5396 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2001 2472 2810 3298 3309 4381 5304
Size: 393 369 471 338 488 11 1072 923
Cuts at: 5304 5654 6461 7058 7845 8113 1239
Size: 350 807 597 787 268 1524
Fragments arranged by size:
1524 1072 923 807 787 597 488 471
393 369 350 338 268 11
EcoO109I rG'GnC_Cy
Cuts at: 2149 2149
Size: 8398
EcoRI G'AATT_C
Cuts at: 2456 2534 5707 6085 2456
Size: 78 3173 378 4769
Fragments arranged by size:
4769 3173 378 78
EcoRV GAT'ATC
Cuts at: 2092 4657 4699 2092
Size: 2565 42 5791
Fragments arranged by size:
5791 2565 42
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3539 4291 3539
Size: 752 7646
FspI TGC'GCA
Cuts at: 6904 6904
Size: 8398
HaeII r_GCGC'y
Cuts at: 1678 7545 1678
Size: 5867 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 1999 2031 4686 4718 4811
Size: 32 135 32 427 32 2655 32 93
Cuts at: 4811 4843 5159 5191 5413 5431 5445 5463 5539
Size: 32 316 32 222 18 14 18 76
Cuts at: 5539 5571 7039 7071 7113 7145 1373
Size: 32 1468 32 42 32 2626
Fragments arranged by size:
2655 2626 1468 427 316 222 135 93
76 42 32 32 32 32 32 32
32 32 32 18 18 14
HincII GTy'rAC
Cuts at: 669 1657 1938 3971 4071 4322 5208 5742 5988
Size: 988 281 2033 100 251 886 534 246
Cuts at: 5988 669
Size: 3079
Fragments arranged by size:
3079 2033 988 886 534 281 251 246
100
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8398
HpaI GTT'AAC
Cuts at: 5988 5988
Size: 8398
KpnI G_GTAC'C
Cuts at: 5723 5723
Size: 8398
MfeI C'AATT_G
Cuts at: 5511 5997 5511
Size: 486 7912
MluI A'CGCG_T
Cuts at: 8274 8274
Size: 8398
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2984 3654 7512 7696 1305
Size: 1679 670 3858 184 2007
Fragments arranged by size:
3858 2007 1679 670 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6576
Fragments arranged by size:
6576 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2543 2723 2948 3429 3831 4207 4916
Size: 648 1377 180 225 481 402 376 709
Cuts at: 4916 4929 5378 6356 6715 6874 518
Size: 13 449 978 359 159 2042
Fragments arranged by size:
2042 1377 978 709 648 481 449 402
376 359 225 180 159 13
MspA1I CmG'CkG
Cuts at: 1606 3287 4649 5814 6491 7639 7884 1606
Size: 1681 1362 1165 677 1148 245 2120
Fragments arranged by size:
2120 1681 1362 1165 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2490 5583 513
Size: 552 1425 3093 3328
Fragments arranged by size:
3328 3093 1425 552
NdeI CA'TA_TG
Cuts at: 387 2418 387
Size: 2031 6367
NheI G'CTAG_C
Cuts at: 1052 5149 1052
Size: 4097 4301
NotI GC'GGCC_GC
Cuts at: 5845 5845
Size: 8398
NsiI A_TGCA'T
Cuts at: 3015 4057 3015
Size: 1042 7356
NspI r_CATG'y
Cuts at: 1132 1229 3434 3457 3900 4180 4432 5269 5756
Size: 97 2205 23 443 280 252 837 487
Cuts at: 5756 7301 1132
Size: 1545 2229
Fragments arranged by size:
2229 2205 1545 837 487 443 280 252
97 23
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8398
PciI A'CATG_T
Cuts at: 4428 7297 4428
Size: 2869 5529
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3527 1108
Size: 2419 5979
PmeI GTTT'AAAC
Cuts at: 5702 5702
Size: 8398
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6467 6499 7980 8012 6467
Size: 32 1481 32 6853
Fragments arranged by size:
6853 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8398
PsiI TTA'TAA
Cuts at: 3072 4683 5469 5968 3072
Size: 1611 786 499 5502
Fragments arranged by size:
5502 1611 786 499
PspOMI G'GGCC_C
Cuts at: 2149 2149
Size: 8398
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2790 2822 2790
Size: 32 8366
PstI C_TGCA'G
Cuts at: 830 3226 4209 4654 5750 830
Size: 2396 983 445 1096 3478
Fragments arranged by size:
3478 2396 1096 983 445
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8398
PvuII CAG'CTG
Cuts at: 3287 4649 3287
Size: 1362 7036
SalI G'TCGA_C
Cuts at: 1936 5740 1936
Size: 3804 4594
SapI GCTCTTCn'nnn_
Cuts at: 3317 3510 5499 3317
Size: 193 1989 6216
Fragments arranged by size:
6216 1989 193
SbfI CC_TGCA'GG
Cuts at: 5750 5750
Size: 8398
ScaI AGT'ACT
Cuts at: 3785 6646 8358 3785
Size: 2861 1712 3825
Fragments arranged by size:
3825 2861 1712
SfcI C'TryA_G
Cuts at: 826 1045 2045 3222 3741 4205 4650 5238 5746
Size: 219 1000 1177 519 464 445 588 508
Cuts at: 5746 6881 7562 7753 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1177 1135 1000 681 588 519 508
464 445 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8398
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8398
SmaI CCC'GGG
Cuts at: 1872 5725 1872
Size: 3853 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 6502 7403 7665 820
Size: 197 926 4559 901 262 1553
Fragments arranged by size:
4559 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6189 493
Size: 5696 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8398
SphI G_CATG'C
Cuts at: 1132 3434 5269 5756 1132
Size: 2302 1835 487 3774
Fragments arranged by size:
3774 2302 1835 487
SspI AAT'ATT
Cuts at: 5 52 2868 3859 6322 5
Size: 47 2816 991 2463 2081
Fragments arranged by size:
2816 2463 2081 991 47
StuI AGG'CCT
Cuts at: 1517 4364 1517
Size: 2847 5551
StyI C'CwwG_G
Cuts at: 513 1065 2490 5583 5837 513
Size: 552 1425 3093 254 3074
Fragments arranged by size:
3093 3074 1425 552 254
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2346 6425 6442 6595 6780 7225 325
Size: 1255 766 4079 17 153 185 445 1498
Fragments arranged by size:
4079 1498 1255 766 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2884 3166 3783
Size: 274 80 33 51 1443 907 282 617
Cuts at: 3783 4211 6644 8356 96
Size: 428 2433 1712 138
Fragments arranged by size:
2433 1712 1443 907 617 428 282 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3440 3472 3627 3659 8358 8390 3440
Size: 32 155 32 4699 32 3448
Fragments arranged by size:
4699 3448 155 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3366 1316
Size: 2050 6348
XbaI T'CTAG_A
Cuts at: 3409 5734 3409
Size: 2325 6073
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8398
XmaI C'CCGG_G
Cuts at: 1870 5723 1870
Size: 3853 4545
XmnI GAAnn'nnTTC
Cuts at: 2513 3213 4017 5676 6527 2513
Size: 700 804 1659 851 4384
Fragments arranged by size:
4384 1659 851 804 700
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6206 276
Size: 53 83 186 936 4672 2468
Fragments arranged by size:
4672 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI BsiWI
BsmBI EcoICRI EcoNI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PpuMI PspXI RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI