Restriction map for
pFN21AB6008
(Circular) (Six-base) MAPSORT of: pFN21AB6008.seq Check: 4386 from: 1 to: 13144
pFN21AB6008 13144 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3119 5147 5276 5464 9089 9284 9368 9817 9881
Size: 2028 129 188 3625 195 84 449 64
Cuts at: 9881 10154 10342 3119
Size: 273 188 5921
Fragments arranged by size:
5921 3625 2028 449 273 195 188 188
129 84 64
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3417 3770 5205 7284
Size: 53 83 186 936 1881 353 1435 2079
Cuts at: 7284 7413 7680 10954 278
Size: 129 267 3274 2468
Fragments arranged by size:
3274 2468 2079 1881 1435 936 353 267
186 129 83 53
AccI GT'mk_AC
Cuts at: 1937 6816 8628 10487 1937
Size: 4879 1812 1859 4594
Fragments arranged by size:
4879 4594 1859 1812
Acc65I G'GTAC_C
Cuts at: 2303 6753 10465 2303
Size: 4450 3712 4982
Fragments arranged by size:
4982 4450 3712
AclI AA'CG_TT
Cuts at: 1563 8127 11271 11644 1563
Size: 6564 3144 373 3063
Fragments arranged by size:
6564 3144 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2532 2708 3205 3398 4374 4976 5604 5912
Size: 900 176 497 193 976 602 628 308
Cuts at: 5912 6094 6150 6236 6411 6643 7335 7760 8775
Size: 182 56 86 175 232 692 425 1015
Cuts at: 8775 10007 10248 11207 12591 12859 1632
Size: 1232 241 959 1384 268 1917
Fragments arranged by size:
1917 1384 1232 1015 976 959 900 692
628 602 497 425 308 268 241 232
193 182 176 175 86 56
AfeI AGC'GCT
Cuts at: 3071 3071
Size: 13144
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 12947
AflIII A'CryG_T
Cuts at: 1203 3837 5331 5471 8912 10157 12043 13020 1203
Size: 2634 1494 140 3441 1245 1886 977 1327
Fragments arranged by size:
3441 2634 1886 1494 1327 1245 977 140
AgeI A'CCGG_T
Cuts at: 11975 11975
Size: 13144
AhdI GACnn_n'nnGTC
Cuts at: 8250 9535 11873 8250
Size: 1285 2338 9521
Fragments arranged by size:
9521 2338 1285
AleI CACnn'nnGTG
Cuts at: 1166 2699 5334 7190 1166
Size: 1533 2635 1856 7120
Fragments arranged by size:
7120 2635 1856 1533
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2212 6164 7075 8974 1184
Size: 1028 3952 911 1899 5354
Fragments arranged by size:
5354 3952 1899 1028 911
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6488 6520 9018 9050 6488
Size: 32 2498 32 10582
Fragments arranged by size:
10582 2498 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2686 2937 2947 3013 3241 3688 6214 6919 7072
Size: 251 10 66 228 447 2526 705 153
Cuts at: 7072 7439 7954 8806 10887 12459 2686
Size: 367 515 852 2081 1572 3371
Fragments arranged by size:
3371 2526 2081 1572 852 705 515 447
367 251 228 153 66 10
ApaI G_GGCC'C
Cuts at: 6258 6443 6474 6899 9777 6258
Size: 185 31 425 2878 9625
Fragments arranged by size:
9625 2878 425 185 31
ApaLI G'TGCA_C
Cuts at: 3322 5451 7852 9355 9802 11201 12357 3322
Size: 2129 2401 1503 447 1399 1156 4109
Fragments arranged by size:
4109 2401 2129 1503 1399 1156 447
ApoI r'AATT_y
Cuts at: 1493 2273 4996 7897 10134 10453 10679 10831 12940
Size: 780 2723 2901 2237 319 226 152 2109
Cuts at: 12940 1493
Size: 1697
Fragments arranged by size:
2901 2723 2237 2109 1697 780 319 226
152
AseI AT'TA_AT
Cuts at: 160 1032 11698 160
Size: 872 10666 1606
Fragments arranged by size:
10666 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 13144
AvaI C'yCGr_G
Cuts at: 1870 1943 2732 3096 3249 3636 4361 4438 4661
Size: 73 789 364 153 387 725 77 223
Cuts at: 4661 5234 6442 6978 7670 8966 9679 9776 10469
Size: 573 1208 536 692 1296 713 97 693
Cuts at: 10469 1870
Size: 4545
Fragments arranged by size:
4545 1296 1208 789 725 713 693 692
573 536 387 364 223 153 97 77
73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2161 2194 2161
Size: 33 13111
BamHI G'GATC_C
Cuts at: 4722 10474 10841 4722
Size: 5752 367 7025
Fragments arranged by size:
7025 5752 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2303 3083 3875 5737 6753
Size: 325 217 627 516 780 792 1862 1016
Cuts at: 6753 6802 6880 7364 7976 8135 9483 10142 10465
Size: 49 78 484 612 159 1348 659 323
Cuts at: 10465 11920 618
Size: 1455 1842
Fragments arranged by size:
1862 1842 1455 1348 1016 792 780 659
627 612 516 484 325 323 217 159
78 49
BanII G_rGCy'C
Cuts at: 1391 2581 3636 4083 4362 4647 5090 5765 5997
Size: 1190 1055 447 279 285 443 675 232
Cuts at: 5997 6174 6258 6443 6450 6474 6899 8681 9777
Size: 177 84 185 7 24 425 1782 1096
Cuts at: 9777 10347 10463 1391
Size: 570 116 4072
Fragments arranged by size:
4072 1782 1190 1096 1055 675 570 447
443 425 285 279 232 185 177 116
84 24 7
BbeI G_GCGC'C
Cuts at: 3879 6806 3879
Size: 2927 10217
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2765 3566 4167 5058 5197 5592 5833
Size: 971 866 801 601 891 139 395 241
Cuts at: 5833 7575 9164 9531 9788 9872 928
Size: 1742 1589 367 257 84 4200
Fragments arranged by size:
4200 1742 1589 971 891 866 801 601
395 367 257 241 139 84
BbvCI CC'TCA_GC
Cuts at: 3479 4262 7772 8297 8855 9706 10113 3479
Size: 783 3510 525 558 851 407 6510
Fragments arranged by size:
6510 3510 851 783 558 525 407
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2098 2132 5297 5331 10169 10203 11335
Size: 34 161 34 3165 34 4838 34 1132
Cuts at: 11335 11369 1903
Size: 34 3678
Fragments arranged by size:
4838 3678 3165 1132 161 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2162 11036 12246 2162
Size: 8874 1210 3060
Fragments arranged by size:
8874 3060 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 13144
BfrBI ATG'CAT
Cuts at: 7360 8146 7360
Size: 786 12358
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3873 3966 4059
Size: 107 122 71 631 605 2201 93 93
Cuts at: 4059 4440 7385 7533 9681 11755 136
Size: 381 2945 148 2148 2074 1525
Fragments arranged by size:
2945 2201 2148 2074 1525 631 605 381
148 122 107 93 93 71
BglII A'GATC_T
Cuts at: 4146 8841 13139 4146
Size: 4695 4298 4151
Fragments arranged by size:
4695 4298 4151
BlpI GC'TnA_GC
Cuts at: 6323 7268 8219 9692 10561 6323
Size: 945 951 1473 869 8906
Fragments arranged by size:
8906 1473 951 945 869
Bme1580I G_kGCm'C
Cuts at: 1790 2833 2891 3326 4113 4127 5341 5384 5455
Size: 1043 58 435 787 14 1214 43 71
Cuts at: 5455 6258 6443 6474 6885 6899 7787 7856 8038
Size: 803 185 31 411 14 888 69 182
Cuts at: 8038 9359 9777 9806 11205 12361 1790
Size: 1321 418 29 1399 1156 2573
Fragments arranged by size:
2573 1399 1321 1214 1156 1043 888 803
787 435 418 411 185 182 71 69
58 43 31 29 14 14
BmgBI CAC'GTC
Cuts at: 1318 3838 6613 7078 7231 8370 1318
Size: 2520 2775 465 153 1139 6092
Fragments arranged by size:
6092 2775 2520 1139 465 153
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2876 3517 4114
Size: 416 53 83 399 24 1459 641 597
Cuts at: 4114 5646 6747 8984 9005 9157 9397 9529 9556
Size: 1532 1101 2237 21 152 240 132 27
Cuts at: 9556 10875 11833 442
Size: 1319 958 1753
Fragments arranged by size:
2237 1753 1532 1459 1319 1101 958 641
597 416 399 240 152 132 83 53
27 24 21
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 13144
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2270 5806 2270
Size: 3536 9608
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2807 2831 4076 4415 4539 5383 5523 5845
Size: 1568 24 1245 339 124 844 140 322
Cuts at: 5845 6362 6885 6941 7087 7541 7976 8010 8405
Size: 517 523 56 146 454 435 34 395
Cuts at: 8405 8454 8602 8703 9020 9680 9875 9989 11804
Size: 49 148 101 317 660 195 114 1815
Cuts at: 11804 1239
Size: 2579
Fragments arranged by size:
2579 1815 1568 1245 844 660 523 517
454 435 395 339 322 317 195 148
146 140 124 114 101 56 49 34
24
Bpu10I CC'TnA_GC
Cuts at: 3479 4262 4937 5484 5661 7772 8297 8855 9706
Size: 783 675 547 177 2111 525 558 851
Cuts at: 9706 10113 3479
Size: 407 6510
Fragments arranged by size:
6510 2111 851 783 675 558 547 525
407 177
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5981 6254 11269 12134 12432 5981
Size: 273 5015 865 298 6693
Fragments arranged by size:
6693 5015 865 298 273
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7811 8095 11807 882
Size: 617 6312 284 3712 2219
Fragments arranged by size:
6312 3712 2219 617 284
BsaAI yAC'GTr
Cuts at: 493 1204 4602 5332 5472 8104 8850 8913 10935
Size: 711 3398 730 140 2632 746 63 2022
Cuts at: 10935 493
Size: 2702
Fragments arranged by size:
3398 2702 2632 2022 746 730 711 140
63
BsaBI GATnn'nnATC
Cuts at: 13138 13138
Size: 13144
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3414 3767 3876 5202
Size: 53 83 186 936 1881 353 109 1326
Cuts at: 5202 6803 7281 7410 7677 10951 11333 11968 275
Size: 1601 478 129 267 3274 382 635 1451
Fragments arranged by size:
3274 1881 1601 1451 1326 936 635 478
382 353 267 186 129 109 83 53
BsaWI w'CCGG_w
Cuts at: 2393 3062 4563 6003 6827 7016 11577 11975 12249
Size: 669 1501 1440 824 189 4561 398 274
Cuts at: 12249 12396 12807 2393
Size: 147 411 2730
Fragments arranged by size:
4561 2730 1501 1440 824 669 411 398
274 189 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3257 3287 3827 3857 4512
Size: 30 165 30 1854 30 540 30 655
Cuts at: 4512 4542 4658 4688 4964 4994 5255 5285 5405
Size: 30 116 30 276 30 261 30 120
Cuts at: 5405 5435 5718 5748 5960 5990 8173 8203 8378
Size: 30 283 30 212 30 2183 30 175
Cuts at: 8378 8408 8507 8537 8663 8681 8693 8711 9018
Size: 30 99 30 126 18 12 18 307
Cuts at: 9018 9048 10157 10187 10301 10331 1178
Size: 30 1109 30 114 30 3991
Fragments arranged by size:
3991 2183 1854 1109 655 540 307 283
276 261 212 175 165 126 120 116
114 99 30 30 30 30 30 30
30 30 30 30 30 30 30 30
30 30 30 18 18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2607 2632 3761 4812 5411 5848 6390 6584
Size: 1421 25 1129 1051 599 437 542 194
Cuts at: 6584 7090 8402 8420 9113 9353 9632 9981 10062
Size: 506 1312 18 693 240 279 349 81
Cuts at: 10062 10178 1186
Size: 116 4152
Fragments arranged by size:
4152 1421 1312 1129 1051 693 599 542
506 437 349 279 240 194 116 81
25 18
BseYI C'CCAG_C
Cuts at: 2375 2621 2777 4900 5340 7111 7304 7506 7528
Size: 246 156 2123 440 1771 193 202 22
Cuts at: 7528 9654 12347 12843 2375
Size: 2126 2693 496 2676
Fragments arranged by size:
2693 2676 2126 2123 1771 496 440 246
202 193 156 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2091 2896 2933 3341 3653 4561 4706
Size: 573 355 805 37 408 312 908 145
Cuts at: 4706 5525 6932 7035 8036 8372 8749 8830 8860
Size: 819 1407 103 1001 336 377 81 30
Cuts at: 8860 9085 9346 9845 9880 10325 10346 10351 10456
Size: 225 261 499 35 445 21 5 105
Cuts at: 10456 1163
Size: 3851
Fragments arranged by size:
3851 1407 1001 908 819 805 573 499
445 408 377 355 336 312 261 225
145 105 103 81 37 35 30 21
5
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3859 4614 6014 6124 9440 10594
Size: 275 186 1858 755 1400 110 3316 1154
Cuts at: 10594 11355 12383 1540
Size: 761 1028 2301
Fragments arranged by size:
3316 2301 1858 1400 1154 1028 761 755
275 186 110
BsiHKAI G_wGCw'C
Cuts at: 2696 3041 3326 3845 4607 5090 5455 5667 7622
Size: 345 285 519 762 483 365 212 1955
Cuts at: 7622 7856 8681 9359 9410 9806 10319 11205 11290
Size: 234 825 678 51 396 513 886 85
Cuts at: 11290 12361 2696
Size: 1071 3479
Fragments arranged by size:
3479 1955 1071 886 825 762 678 519
513 483 396 365 345 285 234 212
85 51
BsmI GAATG_Cn'
Cuts at: 3058 4043 8456 8953 10655 10748 3058
Size: 985 4413 497 1702 93 5454
Fragments arranged by size:
5454 4413 1702 985 497 93
BsmBI CGTCTCn'nnnn_
Cuts at: 6962 7187 7417 9649 9992 6962
Size: 225 230 2232 343 10114
Fragments arranged by size:
10114 2232 343 230 225
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2581 2696 2833 2891 3041 3326 3636
Size: 399 791 115 137 58 150 285 310
Cuts at: 3636 3845 4083 4113 4127 4362 4607 4647 5090
Size: 209 238 30 14 235 245 40 443
Cuts at: 5090 5341 5384 5455 5667 5765 5997 6174 6258
Size: 251 43 71 212 98 232 177 84
Cuts at: 6258 6443 6450 6474 6885 6899 7622 7787 7856
Size: 185 7 24 411 14 723 165 69
Cuts at: 7856 8038 8681 9359 9410 9777 9806 10319 10347
Size: 182 643 678 51 367 29 513 28
Cuts at: 10347 10463 11205 11290 12361 1391
Size: 116 742 85 1071 2174
Fragments arranged by size:
2174 1071 791 742 723 678 643 513
443 411 399 367 310 285 251 245
238 235 232 212 209 185 182 177
165 150 137 116 115 98 85 84
71 69 58 51 43 40 30 29
28 24 14 14 7
BspEI T'CCGG_A
Cuts at: 3062 7016 12807 3062
Size: 3954 5791 3399
Fragments arranged by size:
5791 3954 3399
BspHI T'CATG_A
Cuts at: 5154 9978 11033 12763 5154
Size: 4824 1055 1730 5535
Fragments arranged by size:
5535 4824 1730 1055
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2079 2486 2945 3119 3249 3504 3572 3696
Size: 1235 407 459 174 130 255 68 124
Cuts at: 3696 3825 3987 4016 4353 5147 5276 5464 5579
Size: 129 162 29 337 794 129 188 115
Cuts at: 5579 7847 9012 9089 9284 9368 9817 9818 9881
Size: 2268 1165 77 195 84 449 1 63
Cuts at: 9881 10154 10342 10499 844
Size: 273 188 157 3489
Fragments arranged by size:
3489 2268 1235 1165 794 459 449 407
337 273 255 195 188 188 174 162
157 130 129 129 124 115 84 77
68 63 29 1
BsrBI CCG'CTC
Cuts at: 1394 1823 3330 4014 5920 11031 1394
Size: 429 1507 684 1906 5111 3507
Fragments arranged by size:
5111 3507 1906 1507 684 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3151 4048 5313 6030 11639 11813 65
Size: 1183 1903 897 1265 717 5609 174 1396
Fragments arranged by size:
5609 1903 1396 1265 1183 897 717 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4881 5613 11788 11975 1700
Size: 110 3071 732 6175 187 2869
Fragments arranged by size:
6175 3071 2869 732 187 110
BsrGI T'GTAC_A
Cuts at: 96 2154 96
Size: 2058 11086
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 13144
BssSI C'ACGA_G
Cuts at: 11204 12216 11204
Size: 1012 12132
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2631 3111 3395 6697 7442 8772 9142 9810
Size: 1837 480 284 3302 745 1330 370 668
Cuts at: 9810 9882 794
Size: 72 4056
Fragments arranged by size:
4056 3302 1837 1330 745 668 480 370
284 72
BstBI TT'CG_AA
Cuts at: 11948 11948
Size: 13144
BstEII G'GTnAC_C
Cuts at: 1184 3437 4777 5356 6640 6788 6823 7585 1184
Size: 2253 1340 579 1284 148 35 762 6743
Fragments arranged by size:
6743 2253 1340 1284 762 579 148 35
BstXI CCAn_nnnn'nTGG
Cuts at: 4789 6493 6892 7724 10354 4789
Size: 1704 399 832 2630 7579
Fragments arranged by size:
7579 2630 1704 832 399
BstYI r'GATC_y
Cuts at: 1972 3135 3228 4146 4722 6276 6705 7032 7515
Size: 1163 93 918 576 1554 429 327 483
Cuts at: 7515 8262 8841 10474 10841 11226 11243 12695 12781
Size: 747 579 1633 367 385 17 1452 86
Cuts at: 12781 12793 13139 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1633 1554 1452 1163 918 747 579
576 483 429 385 367 346 327 93
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3000 5626 3000
Size: 2626 10518
BtgI C'CryG_G
Cuts at: 513 1065 2097 3399 3517 3894 4314 4917 4947
Size: 552 1032 1302 118 377 420 603 30
Cuts at: 4947 5955 6486 6645 7235 7581 8895 9521 10407
Size: 1008 531 159 590 346 1314 626 886
Cuts at: 10407 513
Size: 3250
Fragments arranged by size:
3250 1314 1302 1032 1008 886 626 603
590 552 531 420 377 346 159 118
30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2989 4265 6127 9363 487
Size: 683 56 1763 1276 1862 3236 4268
Fragments arranged by size:
4268 3236 1862 1763 1276 683 56
BtsI GCAGTG_nn'
Cuts at: 2593 2681 2918 3989 5099 5513 5530 5665 6334
Size: 88 237 1071 1110 414 17 135 669
Cuts at: 6334 8399 8416 10185 10363 10660 11454 11474 2593
Size: 2065 17 1769 178 297 794 20 4263
Fragments arranged by size:
4263 2065 1769 1110 1071 794 669 414
297 237 178 135 88 20 17 17
ClaI AT'CG_AT
Cuts at: 1554 2424 9795 1554
Size: 870 7371 4903
Fragments arranged by size:
7371 4903 870
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 7371 7406 9238 9273 529
Size: 35 6807 35 1832 35 4400
Fragments arranged by size:
6807 4400 1832 35 35 35
DraI TTT'AAA
Cuts at: 10448 10448
Size: 13144
DraIII CAC_nnn'GTG
Cuts at: 3045 4188 5379 7708 7879 8772 3045
Size: 1143 1191 2329 171 893 7417
Fragments arranged by size:
7417 2329 1191 1143 893 171
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2327 12151 809
Size: 507 1011 9824 1802
Fragments arranged by size:
9824 1802 1011 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2415 3406 3514
Size: 54 1425 50 275 18 585 991 108
Cuts at: 3514 3856 3882 4879 5370 5611 5952 6159 7726
Size: 342 26 997 491 241 341 207 1567
Cuts at: 7726 8357 8898 10591 11480 13109 8
Size: 631 541 1693 889 1629 43
Fragments arranged by size:
1693 1629 1567 1425 997 991 889 631
585 541 491 342 341 275 241 207
108 54 50 43 26 18
EagI C'GGCC_G
Cuts at: 1537 1812 3856 10591 1537
Size: 275 2044 6735 4090
Fragments arranged by size:
6735 4090 2044 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2684 4829 7130 7658 7736 7809 8751 9542
Size: 1331 2145 2301 528 78 73 942 791
Cuts at: 9542 10061 11074 1353
Size: 519 1013 3423
Fragments arranged by size:
3423 2301 2145 1331 1013 942 791 528
519 78 73
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3644 6269 8878 8930 11731 12105
Size: 1064 138 2055 2625 2609 52 2801 374
Cuts at: 12105 12251 387
Size: 146 1280
Fragments arranged by size:
2801 2625 2609 2055 1280 1064 374 146
138 52
EcoICRI GAG'CTC
Cuts at: 5088 8679 5088
Size: 3591 9553
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2532 2708 2807 2831 3205 3398 4076
Size: 393 900 176 99 24 374 193 678
Cuts at: 4076 4374 4415 4539 4976 5383 5523 5604 5845
Size: 298 41 124 437 407 140 81 241
Cuts at: 5845 5912 6094 6150 6236 6362 6411 6643 6885
Size: 67 182 56 86 126 49 232 242
Cuts at: 6885 6941 7087 7335 7541 7760 7976 8010 8405
Size: 56 146 248 206 219 216 34 395
Cuts at: 8405 8454 8602 8703 8775 9020 9680 9875 9989
Size: 49 148 101 72 245 660 195 114
Cuts at: 9989 10007 10248 11207 11804 12591 12859 1239
Size: 18 241 959 597 787 268 1524
Fragments arranged by size:
1524 959 900 787 678 660 597 437
407 395 393 374 298 268 248 245
242 241 241 232 219 216 206 195
193 182 176 148 146 140 126 124
114 101 99 86 81 72 67 56
56 49 49 41 34 24 18
EcoNI CCTnn'n_nnAGG
Cuts at: 4790 6956 4790
Size: 2166 10978
EcoO109I rG'GnC_Cy
Cuts at: 2061 2538 2662 2934 3493 3685 3990 4713 6211
Size: 477 124 272 559 192 305 723 1498
Cuts at: 6211 6255 6298 6470 6896 7175 7301 7951 8425
Size: 44 43 172 426 279 126 650 474
Cuts at: 8425 8461 8818 9664 2061
Size: 36 357 846 5541
Fragments arranged by size:
5541 1498 846 723 650 559 477 474
426 357 305 279 272 192 172 126
124 44 43 36
EcoRI G'AATT_C
Cuts at: 10453 10831 10453
Size: 378 12766
EcoRV GAT'ATC
Cuts at: 10243 10243
Size: 13144
FspI TGC'GCA
Cuts at: 5927 11650 5927
Size: 5723 7421
HaeII r_GCGC'y
Cuts at: 1678 3073 3879 5440 6806 12291 1678
Size: 1395 806 1561 1366 5485 2531
Fragments arranged by size:
5485 2531 1561 1395 1366 806
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3827 3859 4154 4186 5255
Size: 32 135 32 2255 32 295 32 1069
Cuts at: 5255 5287 5843 5875 6656 6688 7085 7117 8236
Size: 32 556 32 781 32 397 32 1119
Cuts at: 8236 8268 8681 8713 9521 9553 9960 9992 10301
Size: 32 413 32 808 32 407 32 309
Cuts at: 10301 10333 11785 11817 11859 11891 1373
Size: 32 1452 32 42 32 2626
Fragments arranged by size:
2626 2255 1452 1119 1069 808 781 556
413 407 397 309 295 135 42 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2134 5578 5695 7756 8125 8578
Size: 988 281 196 3444 117 2061 369 453
Cuts at: 8578 8872 9202 10488 10734 669
Size: 294 330 1286 246 3079
Fragments arranged by size:
3444 3079 2061 1286 988 453 369 330
294 281 246 196 117
HindIII A'AGCT_T
Cuts at: 748 4240 748
Size: 3492 9652
HpaI GTT'AAC
Cuts at: 10734 10734
Size: 13144
KasI G'GCGC_C
Cuts at: 3875 6802 3875
Size: 2927 10217
KpnI G_GTAC'C
Cuts at: 2307 6757 10469 2307
Size: 4450 3712 4982
Fragments arranged by size:
4982 4450 3712
MfeI C'AATT_G
Cuts at: 10743 10743
Size: 13144
MluI A'CGCG_T
Cuts at: 13020 13020
Size: 13144
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7479 7566 8233 8537 8715 10355 12258 12442
Size: 6174 87 667 304 178 1640 1903 184
Cuts at: 12442 1305
Size: 2007
Fragments arranged by size:
6174 2007 1903 1640 667 304 184 178
87
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2417 3408 3884 6161 7728
Size: 54 1425 343 585 991 476 2277 1567
Cuts at: 7728 10
Size: 5426
Fragments arranged by size:
5426 2277 1567 1425 991 585 476 343
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2699 2955 4406 4590 4787 4946 5334
Size: 648 1533 256 1451 184 197 159 388
Cuts at: 5334 5685 6875 6890 7190 7229 7974 8021 8066
Size: 351 1190 15 300 39 745 47 45
Cuts at: 8066 8664 9368 10352 11102 11461 11620 518
Size: 598 704 984 750 359 159 2042
Fragments arranged by size:
2042 1533 1451 1190 984 750 745 704
648 598 388 359 351 300 256 197
184 159 159 47 45 39 15
MspA1I CmG'CkG
Cuts at: 1606 2433 2712 2994 3519 3808 3896 3945 4337
Size: 827 279 282 525 289 88 49 392
Cuts at: 4337 5448 6583 6694 7291 7439 7833 7917 8356
Size: 1111 1135 111 597 148 394 84 439
Cuts at: 8356 8745 8859 9432 9523 9658 9691 9816 10560
Size: 389 114 573 91 135 33 125 744
Cuts at: 10560 11237 12385 12630 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 1135 1111 827 744 677 597
573 525 439 394 392 389 289 282
279 245 148 135 125 114 111 91
88 84 49 33
NaeI GCC'GGC
Cuts at: 5615 5615
Size: 13144
NarI GG'CG_CC
Cuts at: 3876 6803 3876
Size: 2927 10217
NcoI C'CATG_G
Cuts at: 513 1065 3399 4314 6486 7235 7581 10407 513
Size: 552 2334 915 2172 749 346 2826 3250
Fragments arranged by size:
3250 2826 2334 2172 915 749 552 346
NdeI CA'TA_TG
Cuts at: 387 387
Size: 13144
NgoMIV G'CCGG_C
Cuts at: 5613 5613
Size: 13144
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 13144
NotI GC'GGCC_GC
Cuts at: 10591 10591
Size: 13144
NsiI A_TGCA'T
Cuts at: 7362 8148 7362
Size: 786 12358
NspI r_CATG'y
Cuts at: 1132 1229 2155 9754 10161 10502 12047 1132
Size: 97 926 7599 407 341 1545 2229
Fragments arranged by size:
7599 2229 1545 926 407 341 97
PasI CC'CwG_GG
Cuts at: 1348 4076 4709 6466 9889 1348
Size: 2728 633 1757 3423 4603
Fragments arranged by size:
4603 3423 2728 1757 633
PciI A'CATG_T
Cuts at: 10157 12043 10157
Size: 1886 11258
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2574 2722 7072 8322 8806 1108
Size: 1466 148 4350 1250 484 5446
Fragments arranged by size:
5446 4350 1466 1250 484 148
PfoI T'CCnGG_A
Cuts at: 2808 3012 5075 5208 5409 5550 6185 6510 6957
Size: 204 2063 133 201 141 635 325 447
Cuts at: 6957 7089 7248 8382 8805 8820 9542 10005 2808
Size: 132 159 1134 423 15 722 463 5947
Fragments arranged by size:
5947 2063 1134 722 635 463 447 423
325 204 201 159 141 133 132 15
PmeI GTTT'AAAC
Cuts at: 10448 10448
Size: 13144
PmlI CAC'GTG
Cuts at: 4602 5332 5472 8850 8913 4602
Size: 730 140 3378 63 8833
Fragments arranged by size:
8833 3378 730 140 63
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 9018 9050 11213 11245 12726 12758 9018
Size: 32 2163 32 1481 32 9404
Fragments arranged by size:
9404 2163 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2061 2538 2934 3493 3685 3990 4713 7951 8425
Size: 477 396 559 192 305 723 3238 474
Cuts at: 8425 8461 8818 9664 2061
Size: 36 357 846 5541
Fragments arranged by size:
5541 3238 846 723 559 477 474 396
357 305 192 36
PshAI GACnn'nnGTC
Cuts at: 1613 9432 1613
Size: 7819 5325
PsiI TTA'TAA
Cuts at: 10714 10714
Size: 13144
PspOMI G'GGCC_C
Cuts at: 6254 6439 6470 6895 9773 6254
Size: 185 31 425 2878 9625
Fragments arranged by size:
9625 2878 425 185 31
PspXI vC'TCGA_Gb
Cuts at: 4361 4361
Size: 13144
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3815 3847 3815
Size: 32 13112
PstI C_TGCA'G
Cuts at: 830 2177 2993 3143 3501 3693 3984 5822 6627
Size: 1347 816 150 358 192 291 1838 805
Cuts at: 6627 6693 7005 7032 7064 7206 7985 8217 9092
Size: 66 312 27 32 142 779 232 875
Cuts at: 9092 10200 10496 830
Size: 1108 296 3478
Fragments arranged by size:
3478 1838 1347 1108 875 816 805 779
358 312 296 291 232 192 150 142
66 32 27
PvuI CG_AT'CG
Cuts at: 2001 4614 2001
Size: 2613 10531
PvuII CAG'CTG
Cuts at: 2994 5448 7439 7917 8745 9432 9658 9691 2994
Size: 2454 1991 478 828 687 226 33 6447
Fragments arranged by size:
6447 2454 1991 828 687 478 226 33
SacI G_AGCT'C
Cuts at: 5090 8681 5090
Size: 3591 9553
SacII CC_GC'GG
Cuts at: 3520 3897 9524 3520
Size: 377 5627 7140
Fragments arranged by size:
7140 5627 377
SalI G'TCGA_C
Cuts at: 1936 10486 1936
Size: 8550 4594
SanDI GG'GwC_CC
Cuts at: 2061 3990 8818 2061
Size: 1929 4828 6387
Fragments arranged by size:
6387 4828 1929
SapI GCTCTTCn'nnn_
Cuts at: 2684 2684
Size: 13144
SbfI CC_TGCA'GG
Cuts at: 9092 10496 9092
Size: 1404 11740
ScaI AGT'ACT
Cuts at: 6629 10055 11392 13104 6629
Size: 3426 1337 1712 6669
Fragments arranged by size:
6669 3426 1712 1337
SexAI A'CCwGG_T
Cuts at: 3734 5835 6810 6993 3734
Size: 2101 975 183 9885
Fragments arranged by size:
9885 2101 975 183
SfcI C'TryA_G
Cuts at: 826 1045 2173 2989 3139 3497 3689 3980 5182
Size: 219 1128 816 150 358 192 291 1202
Cuts at: 5182 5818 6623 6689 7001 7028 7060 7202 7981
Size: 636 805 66 312 27 32 142 779
Cuts at: 7981 8213 9088 10196 10492 11627 12308 12499 826
Size: 232 875 1108 296 1135 681 191 1471
Fragments arranged by size:
1471 1202 1135 1128 1108 875 816 805
779 681 636 358 312 296 291 232
219 192 191 150 142 66 32 27
SfoI GGC'GCC
Cuts at: 3877 6804 3877
Size: 2927 10217
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 13144
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 13144
SmaI CCC'GGG
Cuts at: 1872 3251 3638 4440 4663 6444 7672 9778 10471
Size: 1379 387 802 223 1781 1228 2106 693
Cuts at: 10471 1872
Size: 4545
Fragments arranged by size:
4545 2106 1781 1379 1228 802 693 387
223
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3096 4361 5996 6269 11248 12149
Size: 197 926 1153 1265 1635 273 4979 901
Cuts at: 12149 12411 820
Size: 262 1553
Fragments arranged by size:
4979 1635 1553 1265 1153 926 901 273
262 197
SnaBI TAC'GTA
Cuts at: 493 10935 493
Size: 10442 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 13144
SphI G_CATG'C
Cuts at: 1132 9754 10502 1132
Size: 8622 748 3774
Fragments arranged by size:
8622 3774 748
SspI AAT'ATT
Cuts at: 5 52 11068 5
Size: 47 11016 2081
Fragments arranged by size:
11016 2081 47
StuI AGG'CCT
Cuts at: 1517 2402 3024 3785 4049 5730 5897 6314 7777
Size: 885 622 761 264 1681 167 417 1463
Cuts at: 7777 8762 9746 1517
Size: 985 984 4915
Fragments arranged by size:
4915 1681 1463 985 984 885 761 622
417 264 167
StyI C'CwwG_G
Cuts at: 513 1065 3399 4113 4314 6486 7146 7235 7323
Size: 552 2334 714 201 2172 660 89 88
Cuts at: 7323 7581 7590 8167 9223 10407 10583 513
Size: 258 9 577 1056 1184 176 3074
Fragments arranged by size:
3074 2334 2172 1184 1056 714 660 577
552 258 201 176 89 88 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2876 8096 9426 11171 11188 11341 11526
Size: 1255 1296 5220 1330 1745 17 153 185
Cuts at: 11526 11971 325
Size: 445 1498
Fragments arranged by size:
5220 1745 1498 1330 1296 1255 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2154 6627 10053
Size: 274 80 33 51 1443 177 4473 3426
Cuts at: 10053 10281 11390 13102 96
Size: 228 1109 1712 138
Fragments arranged by size:
4473 3426 1712 1443 1109 274 228 177
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2053 2085 3031 3063 3257 3289 4512 4544 4553
Size: 32 946 32 194 32 1223 32 9
Cuts at: 4553 4585 4964 4996 5632 5664 5718 5750 5960
Size: 32 379 32 636 32 54 32 210
Cuts at: 5960 5992 6817 6849 8173 8205 8378 8410 8507
Size: 32 825 32 1324 32 173 32 97
Cuts at: 8507 8539 9265 9297 10157 10189 13104 13136 2053
Size: 32 726 32 860 32 2915 32 2061
Fragments arranged by size:
2915 2061 1324 1223 946 860 825 726
636 379 210 194 173 97 54 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 9
Tth111I GACn'n_nGTC
Cuts at: 1316 2129 4256 5837 1316
Size: 813 2127 1581 8623
Fragments arranged by size:
8623 2127 1581 813
XbaI T'CTAG_A
Cuts at: 2362 10480 2362
Size: 8118 5026
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4321 4615 5776 6739 7588 8642 9319 4321
Size: 294 1161 963 849 1054 677 8146
Fragments arranged by size:
8146 1161 1054 963 849 677 294
XhoI C'TCGA_G
Cuts at: 1943 3096 4361 1943
Size: 1153 1265 10726
Fragments arranged by size:
10726 1265 1153
XmaI C'CCGG_G
Cuts at: 1870 3249 3636 4438 4661 6442 7670 9776 10469
Size: 1379 387 802 223 1781 1228 2106 693
Cuts at: 10469 1870
Size: 4545
Fragments arranged by size:
4545 2106 1781 1379 1228 802 693 387
223
XmnI GAAnn'nnTTC
Cuts at: 7139 11273 7139
Size: 4134 9010
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3415 3768 5203 7282
Size: 53 83 186 936 1881 353 1435 2079
Cuts at: 7282 7411 7678 10952 276
Size: 129 267 3274 2468
Fragments arranged by size:
3274 2468 2079 1881 1435 936 353 267
186 129 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BsiWI BstZ17I FalI FseI FspAI NruI
PacI RsrII SfiI SrfI SwaI