Restriction map for
pFN21AB6007
(Circular) (Six-base) MAPSORT of: pFN21AB6007.seq Check: 9769 from: 1 to: 7207
pFN21AB6007 7207 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3423 3530 5017 278
Size: 53 83 186 936 1887 107 1487 2468
Fragments arranged by size:
2468 1887 1487 936 186 107 83 53
AccI GT'mk_AC
Cuts at: 1937 2868 4059 4550 1937
Size: 931 1191 491 4594
Fragments arranged by size:
4594 1191 931 491
Acc65I G'GTAC_C
Cuts at: 4528 4528
Size: 7207
AclI AA'CG_TT
Cuts at: 1563 5334 5707 1563
Size: 3771 373 3063
Fragments arranged by size:
3771 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2515 2732 3212 5270 6654 6922 1632
Size: 883 217 480 2058 1384 268 1917
Fragments arranged by size:
2058 1917 1384 883 480 268 217
AfeI AGC'GCT
Cuts at: 3401 3779 3401
Size: 378 6829
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7010
AflIII A'CryG_T
Cuts at: 1203 2883 3936 6106 7083 1203
Size: 1680 1053 2170 977 1327
Fragments arranged by size:
2170 1680 1327 1053 977
AgeI A'CCGG_T
Cuts at: 6038 6038
Size: 7207
AhdI GACnn_n'nnGTC
Cuts at: 5936 5936
Size: 7207
AleI CACnn'nnGTG
Cuts at: 1166 3434 4025 1166
Size: 2268 591 4348
Fragments arranged by size:
4348 2268 591
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3065 3669 1184
Size: 1881 604 4722
Fragments arranged by size:
4722 1881 604
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2321 2353 2730 2762 2321
Size: 32 377 32 6766
Fragments arranged by size:
6766 377 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2423 2960 4950 6522 2423
Size: 537 1990 1572 3108
Fragments arranged by size:
3108 1990 1572 537
ApaI G_GGCC'C
Cuts at: 3766 3766
Size: 7207
ApaLI G'TGCA_C
Cuts at: 2384 5264 6420 2384
Size: 2880 1156 3171
Fragments arranged by size:
3171 2880 1156
ApoI r'AATT_y
Cuts at: 1493 2208 2726 3632 3793 3830 4516 4742 4894
Size: 715 518 906 161 37 686 226 152
Cuts at: 4894 7003 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 906 715 686 518 226 161
152 37
AseI AT'TA_AT
Cuts at: 160 1032 5761 160
Size: 872 4729 1606
Fragments arranged by size:
4729 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7207
AvaI C'yCGr_G
Cuts at: 1870 1943 2086 2805 3694 4532 1870
Size: 73 143 719 889 838 4545
Fragments arranged by size:
4545 889 838 719 143 73
BamHI G'GATC_C
Cuts at: 2745 4537 4904 2745
Size: 1792 367 5048
Fragments arranged by size:
5048 1792 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3049 4105 4528 5983 618
Size: 325 217 627 1262 1056 423 1455 1842
Fragments arranged by size:
1842 1455 1262 1056 627 423 325 217
BanII G_rGCy'C
Cuts at: 1391 2028 2849 2949 3766 4526 1391
Size: 637 821 100 817 760 4072
Fragments arranged by size:
4072 821 817 760 637 100
BbeI G_GCGC'C
Cuts at: 4109 4109
Size: 7207
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2026 2199 2555 2573 2667 2883 3307
Size: 971 127 173 356 18 94 216 424
Cuts at: 3307 4421 928
Size: 1114 3714
Fragments arranged by size:
3714 1114 971 424 356 216 173 127
94 18
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3862 3896 5398 5432 1903
Size: 34 1925 34 1502 34 3678
Fragments arranged by size:
3678 1925 1502 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5099 6309 5099
Size: 1210 5997
BclI T'GATC_A
Cuts at: 1548 3753 1548
Size: 2205 5002
BfrBI ATG'CAT
Cuts at: 3172 3172
Size: 7207
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5818 136
Size: 107 122 71 631 605 4146 1525
Fragments arranged by size:
4146 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7202 7202
Size: 7207
BlpI GC'TnA_GC
Cuts at: 2402 4624 2402
Size: 2222 4985
Bme1580I G_kGCm'C
Cuts at: 1790 2388 3766 5268 6424 1790
Size: 598 1378 1502 1156 2573
Fragments arranged by size:
2573 1502 1378 1156 598
BmgBI CAC'GTC
Cuts at: 1318 2665 1318
Size: 1347 5860
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2433 2575 4384
Size: 416 53 83 399 24 1016 142 1809
Cuts at: 4384 4938 5896 442
Size: 554 958 1753
Fragments arranged by size:
1809 1753 1016 958 554 416 399 142
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7207
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2708 2708
Size: 7207
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2411 2783 3929 4256 5867 1239
Size: 1172 372 1146 327 1611 2579
Fragments arranged by size:
2579 1611 1172 1146 372 327
Bpu10I CC'TnA_GC
Cuts at: 4079 4079
Size: 7207
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5332 6197 6495 5332
Size: 865 298 6044
Fragments arranged by size:
6044 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3143 3699 4322 4481 5870 882
Size: 617 1644 556 623 159 1389 2219
Fragments arranged by size:
2219 1644 1389 623 617 556 159
BsaAI yAC'GTr
Cuts at: 493 1204 2353 2884 4998 493
Size: 711 1149 531 2114 2702
Fragments arranged by size:
2702 2114 1149 711 531
BsaBI GATnn'nnATC
Cuts at: 2054 7201 2054
Size: 5147 2060
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3131 3420 3527 4106
Size: 53 83 186 936 1598 289 107 579
Cuts at: 4106 5014 5396 6031 275
Size: 908 382 635 1451
Fragments arranged by size:
1598 1451 936 908 635 579 382 289
186 107 83 53
BsaWI w'CCGG_w
Cuts at: 2262 5640 6038 6312 6459 6870 2262
Size: 3378 398 274 147 411 2599
Fragments arranged by size:
3378 2599 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2209 2239 2831 2861 2936
Size: 30 165 30 806 30 592 30 75
Cuts at: 2936 2966 3902 3932 1178
Size: 30 936 30 4453
Fragments arranged by size:
4453 936 806 592 165 75 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2153 2669 3221 3926 3929 4307 1186
Size: 967 516 552 705 3 378 4086
Fragments arranged by size:
4086 967 705 552 516 378 3
BseYI C'CCAG_C
Cuts at: 4153 4358 6410 6906 4153
Size: 205 2052 496 4454
Fragments arranged by size:
4454 2052 496 205
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2101 2393 4123 1163
Size: 573 365 292 1730 4247
Fragments arranged by size:
4247 1730 573 365 292
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3009 3354 4657 5418 6446 1540
Size: 275 186 1008 345 1303 761 1028 2301
Fragments arranged by size:
2301 1303 1028 1008 761 345 275 186
BsiHKAI G_wGCw'C
Cuts at: 2388 4386 4440 5268 5353 6424 2388
Size: 1998 54 828 85 1071 3171
Fragments arranged by size:
3171 1998 1071 828 85 54
BsmI GAATG_Cn'
Cuts at: 2704 3873 4718 4811 2704
Size: 1169 845 93 5100
Fragments arranged by size:
5100 1169 845 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2247 4184 2247
Size: 1937 5270
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2028 2388 2849 2949 3766 4386 4440
Size: 399 238 360 461 100 817 620 54
Cuts at: 4440 4526 5268 5353 6424 1391
Size: 86 742 85 1071 2174
Fragments arranged by size:
2174 1071 817 742 620 461 399 360
238 100 86 85 54
BspEI T'CCGG_A
Cuts at: 2262 6870 2262
Size: 4608 2599
BspHI T'CATG_A
Cuts at: 3750 5096 6826 3750
Size: 1346 1730 4131
Fragments arranged by size:
4131 1730 1346
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2928 3186 4562 844
Size: 2084 258 1376 3489
Fragments arranged by size:
3489 2084 1376 258
BsrBI CCG'CTC
Cuts at: 1394 1823 4083 5094 1394
Size: 429 2260 1011 3507
Fragments arranged by size:
3507 2260 1011 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2498 5702 5876 65
Size: 1183 1250 3204 174 1396
Fragments arranged by size:
3204 1396 1250 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5851 6038 1700
Size: 110 4041 187 2869
Fragments arranged by size:
4041 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3969 4221 96
Size: 3873 252 3082
Fragments arranged by size:
3873 3082 252
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7207
BssSI C'ACGA_G
Cuts at: 5267 6279 5267
Size: 1012 6195
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3178 794
Size: 2384 4823
BstBI TT'CG_AA
Cuts at: 6011 6011
Size: 7207
BstEII G'GTnAC_C
Cuts at: 1184 2608 1184
Size: 1424 5783
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2161 3160 2011
Size: 150 999 6058
Fragments arranged by size:
6058 999 150
BstYI r'GATC_y
Cuts at: 1972 2745 3679 4537 4904 5289 5306 6758 6844
Size: 773 934 858 367 385 17 1452 86
Cuts at: 6844 6856 7202 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 934 858 773 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 2869 2869
Size: 7207
Bsu36I CC'TnA_GG
Cuts at: 3455 3455
Size: 7207
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5716
Fragments arranged by size:
5716 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3052 4339 487
Size: 683 56 1826 1287 3355
Fragments arranged by size:
3355 1826 1287 683 56
BtsI GCAGTG_nn'
Cuts at: 3429 3441 3903 4723 5517 5537 3429
Size: 12 462 820 794 20 5099
Fragments arranged by size:
5099 820 794 462 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7207
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7172
DraI TTT'AAA
Cuts at: 4511 4511
Size: 7207
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2426 3419 6214 809
Size: 507 1110 993 2795 1802
Fragments arranged by size:
2795 1802 1110 993 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2289 3006 3393
Size: 54 1425 50 275 18 459 717 387
Cuts at: 3393 4084 4654 5543 7172 8
Size: 691 570 889 1629 43
Fragments arranged by size:
1629 1425 889 717 691 570 459 387
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3006 4654 1537
Size: 275 1194 1648 4090
Fragments arranged by size:
4090 1648 1194 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2555 3032 3497 3697 3797 4217 5137 1353
Size: 1202 477 465 200 100 420 920 3423
Fragments arranged by size:
3423 1202 920 477 465 420 200 100
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4060 5794 6168 6314 387
Size: 1064 138 2471 1734 374 146 1280
Fragments arranged by size:
2471 1734 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2411 2515 2732 2783 3212 3929 4256
Size: 393 779 104 217 51 429 717 327
Cuts at: 4256 5270 5867 6654 6922 1239
Size: 1014 597 787 268 1524
Fragments arranged by size:
1524 1014 787 779 717 597 429 393
327 268 217 104 51
EcoNI CCTnn'n_nnAGG
Cuts at: 2132 2978 3383 2132
Size: 846 405 5956
Fragments arranged by size:
5956 846 405
EcoO109I rG'GnC_Cy
Cuts at: 2470 2525 2917 3097 3762 2470
Size: 55 392 180 665 5915
Fragments arranged by size:
5915 665 392 180 55
EcoRI G'AATT_C
Cuts at: 2726 3632 4516 4894 2726
Size: 906 884 378 5039
Fragments arranged by size:
5039 906 884 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2323 2659 3691 2323
Size: 336 1032 5839
Fragments arranged by size:
5839 1032 336
FspI TGC'GCA
Cuts at: 5713 5713
Size: 7207
HaeII r_GCGC'y
Cuts at: 1678 3403 3781 4051 4109 6354 1678
Size: 1725 378 270 58 2245 2531
Fragments arranged by size:
2531 2245 1725 378 270 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2209 2241 2348 2380 2560
Size: 32 135 32 637 32 107 32 180
Cuts at: 2560 2592 2831 2863 2936 2968 3902 3934 4480
Size: 32 239 32 73 32 934 32 546
Cuts at: 4480 4512 5848 5880 5922 5954 1373
Size: 32 1336 32 42 32 2626
Fragments arranged by size:
2626 1336 934 637 546 239 180 135
107 73 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2689 4551 4797 669
Size: 988 281 751 1862 246 3079
Fragments arranged by size:
3079 1862 988 751 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7207
HpaI GTT'AAC
Cuts at: 4797 4797
Size: 7207
KasI G'GCGC_C
Cuts at: 4105 4105
Size: 7207
KpnI G_GTAC'C
Cuts at: 4532 4532
Size: 7207
MfeI C'AATT_G
Cuts at: 4806 4806
Size: 7207
MluI A'CGCG_T
Cuts at: 7083 7083
Size: 7207
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4137 6321 6505 1305
Size: 2832 2184 184 2007
Fragments arranged by size:
2832 2184 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2291 10
Size: 54 1425 343 459 4926
Fragments arranged by size:
4926 1425 459 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3434 4025 5165 5524 5683 518
Size: 648 843 1425 591 1140 359 159 2042
Fragments arranged by size:
2042 1425 1140 843 648 591 359 159
MspA1I CmG'CkG
Cuts at: 1606 2344 3016 4168 4623 5300 6448 6693 1606
Size: 738 672 1152 455 677 1148 245 2120
Fragments arranged by size:
2120 1152 1148 738 677 672 455 245
NarI GG'CG_CC
Cuts at: 4106 4106
Size: 7207
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5716
Fragments arranged by size:
5716 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7207
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7207
NotI GC'GGCC_GC
Cuts at: 4654 4654
Size: 7207
NsiI A_TGCA'T
Cuts at: 3174 3174
Size: 7207
NspI r_CATG'y
Cuts at: 1132 1229 3185 3940 4565 6110 1132
Size: 97 1956 755 625 1545 2229
Fragments arranged by size:
2229 1956 1545 755 625 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7207
PciI A'CATG_T
Cuts at: 3936 6106 3936
Size: 2170 5037
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2974 4005 1108
Size: 1866 1031 4310
Fragments arranged by size:
4310 1866 1031
PfoI T'CCnGG_A
Cuts at: 3381 3405 3530 3381
Size: 24 125 7058
Fragments arranged by size:
7058 125 24
PmeI GTTT'AAAC
Cuts at: 4511 4511
Size: 7207
PmlI CAC'GTG
Cuts at: 2353 2884 2353
Size: 531 6676
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5276 5308 6789 6821 5276
Size: 32 1481 32 5662
Fragments arranged by size:
5662 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2525 2917 3097 2525
Size: 392 180 6635
Fragments arranged by size:
6635 392 180
PshAI GACnn'nnGTC
Cuts at: 1613 4307 1613
Size: 2694 4513
PsiI TTA'TAA
Cuts at: 4777 4777
Size: 7207
PspOMI G'GGCC_C
Cuts at: 3762 3762
Size: 7207
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2852 2884 4289 4321 2852
Size: 32 1405 32 5738
Fragments arranged by size:
5738 1405 32 32
PstI C_TGCA'G
Cuts at: 830 2349 3436 4559 830
Size: 1519 1087 1123 3478
Fragments arranged by size:
3478 1519 1123 1087
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7207
PvuII CAG'CTG
Cuts at: 2344 4168 2344
Size: 1824 5383
SalI G'TCGA_C
Cuts at: 1936 4549 1936
Size: 2613 4594
SapI GCTCTTCn'nnn_
Cuts at: 3032 3032
Size: 7207
SbfI CC_TGCA'GG
Cuts at: 4559 4559
Size: 7207
ScaI AGT'ACT
Cuts at: 5455 7167 5455
Size: 1712 5495
SexAI A'CCwGG_T
Cuts at: 2333 2333
Size: 7207
SfcI C'TryA_G
Cuts at: 826 1045 2345 2620 2626 3432 3868 4099 4555
Size: 219 1300 275 6 806 436 231 456
Cuts at: 4555 5690 6371 6562 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1300 1135 806 681 456 436 275
231 219 191 6
SfoI GGC'GCC
Cuts at: 4107 4107
Size: 7207
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7207
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7207
SmaI CCC'GGG
Cuts at: 1872 2088 2807 4534 1872
Size: 216 719 1727 4545
Fragments arranged by size:
4545 1727 719 216
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5311 6212 6474 820
Size: 197 926 3368 901 262 1553
Fragments arranged by size:
3368 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4998 493
Size: 4505 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7207
SphI G_CATG'C
Cuts at: 1132 4565 1132
Size: 3433 3774
SspI AAT'ATT
Cuts at: 5 52 5131 5
Size: 47 5079 2081
Fragments arranged by size:
5079 2081 47
StuI AGG'CCT
Cuts at: 1517 4253 1517
Size: 2736 4471
StyI C'CwwG_G
Cuts at: 513 1065 2004 3766 3999 4646 513
Size: 552 939 1762 233 647 3074
Fragments arranged by size:
3074 1762 939 647 552 233
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5234 5251 5404 5589 6034 325
Size: 1255 3654 17 153 185 445 1498
Fragments arranged by size:
3654 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3969 4221 5453
Size: 274 80 33 51 1443 1992 252 1232
Cuts at: 5453 7165 96
Size: 1712 138
Fragments arranged by size:
1992 1712 1443 1232 274 252 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7167 7199 7167
Size: 32 7175
Tth111I GACn'n_nGTC
Cuts at: 1316 3311 1316
Size: 1995 5212
XbaI T'CTAG_A
Cuts at: 4543 4543
Size: 7207
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2515 3988 4165 2515
Size: 1473 177 5557
Fragments arranged by size:
5557 1473 177
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7207
XmaI C'CCGG_G
Cuts at: 1870 2086 2805 4532 1870
Size: 216 719 1727 4545
Fragments arranged by size:
4545 1727 719 216
XmnI GAAnn'nnTTC
Cuts at: 3671 5336 3671
Size: 1665 5542
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3421 3528 5015 276
Size: 53 83 186 936 1887 107 1487 2468
Fragments arranged by size:
2468 1887 1487 936 186 107 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbvCI BsiWI DraIII EcoICRI
EcoRV FseI FspAI NaeI NgoMIV NruI PacI PspXI
RsrII SacI SacII SanDI SfiI SrfI SwaI