Restriction map for
pFN21AB6005
(Circular) (Six-base) MAPSORT of: pFN21AB6005.seq Check: 4743 from: 1 to: 7954
pFN21AB6005 7954 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4133 4133
Size: 7954
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4869 5764 278
Size: 53 83 186 936 3333 895 2468
Fragments arranged by size:
3333 2468 936 895 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2327 3617 5297 1937
Size: 390 1290 1680 4594
Fragments arranged by size:
4594 1680 1290 390
Acc65I G'GTAC_C
Cuts at: 5275 5275
Size: 7954
AclI AA'CG_TT
Cuts at: 1563 6081 6454 1563
Size: 4518 373 3063
Fragments arranged by size:
4518 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2241 2538 3094 3159 3794 3843 3867 5210
Size: 609 297 556 65 635 49 24 1343
Cuts at: 5210 6017 7401 7669 1632
Size: 807 1384 268 1917
Fragments arranged by size:
1917 1384 1343 807 635 609 556 297
268 65 49 24
AflII C'TTAA_G
Cuts at: 820 1017 5159 820
Size: 197 4142 3615
Fragments arranged by size:
4142 3615 197
AflIII A'CryG_T
Cuts at: 1203 4170 6853 7830 1203
Size: 2967 2683 977 1327
Fragments arranged by size:
2967 2683 1327 977
AgeI A'CCGG_T
Cuts at: 6785 6785
Size: 7954
AhdI GACnn_n'nnGTC
Cuts at: 6683 6683
Size: 7954
AleI CACnn'nnGTG
Cuts at: 1166 3125 3890 4959 1166
Size: 1959 765 1069 4161
Fragments arranged by size:
4161 1959 1069 765
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7954
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4032 4064 4032
Size: 32 7922
AlwNI CAG_nnn'CTG
Cuts at: 2711 3131 4406 4502 5697 7269 2711
Size: 420 1275 96 1195 1572 3396
Fragments arranged by size:
3396 1572 1275 1195 420 96
ApaI G_GGCC'C
Cuts at: 2012 2667 4439 2012
Size: 655 1772 5527
Fragments arranged by size:
5527 1772 655
ApaLI G'TGCA_C
Cuts at: 6011 7167 6011
Size: 1156 6798
ApoI r'AATT_y
Cuts at: 1493 3774 5263 5489 5641 7750 1493
Size: 2281 1489 226 152 2109 1697
Fragments arranged by size:
2281 2109 1697 1489 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6508 160
Size: 872 5476 1606
Fragments arranged by size:
5476 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7954
AvaI C'yCGr_G
Cuts at: 1870 1943 2268 2697 4095 5279 1870
Size: 73 325 429 1398 1184 4545
Fragments arranged by size:
4545 1398 1184 429 325 73
AvrII C'CTAG_G
Cuts at: 4133 4133
Size: 7954
BamHI G'GATC_C
Cuts at: 3336 3684 5284 5651 3336
Size: 348 1600 367 5639
Fragments arranged by size:
5639 1600 367 348
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2019 3304 4145 4595 5275
Size: 325 217 627 232 1285 841 450 680
Cuts at: 5275 6730 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1285 841 680 627 450 325
232 217
BanII G_rGCy'C
Cuts at: 1391 2012 2116 2667 2899 3995 4439 5273 1391
Size: 621 104 551 232 1096 444 834 4072
Fragments arranged by size:
4072 1096 834 621 551 444 232 104
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4697 928
Size: 971 2798 4185
Fragments arranged by size:
4185 2798 971
BbvCI CC'TCA_GC
Cuts at: 3840 4071 3840
Size: 231 7723
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6145 6179 1903
Size: 34 4208 34 3678
Fragments arranged by size:
4208 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3564 3725 3738 4222 4936 5846 7056 3564
Size: 161 13 484 714 910 1210 4462
Fragments arranged by size:
4462 1210 910 714 484 161 13
BclI T'GATC_A
Cuts at: 1548 4228 1548
Size: 2680 5274
BfrBI ATG'CAT
Cuts at: 3659 3659
Size: 7954
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6565 136
Size: 107 122 71 631 605 4893 1525
Fragments arranged by size:
4893 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7949 7949
Size: 7954
BlpI GC'TnA_GC
Cuts at: 2129 2787 5371 2129
Size: 658 2584 4712
Fragments arranged by size:
4712 2584 658
Bme1580I G_kGCm'C
Cuts at: 1790 2012 2667 3309 4439 6015 7171 1790
Size: 222 655 642 1130 1576 1156 2573
Fragments arranged by size:
2573 1576 1156 1130 655 642 222
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7954
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2592 3007 3284
Size: 416 53 83 399 24 1175 415 277
Cuts at: 3284 4772 4963 5685 6643 442
Size: 1488 191 722 958 1753
Fragments arranged by size:
1753 1488 1175 958 722 416 415 399
277 191 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3972 1056
Size: 2916 5038
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2246 2889 3912 4365 6614 1239
Size: 1007 643 1023 453 2249 2579
Fragments arranged by size:
2579 2249 1023 1007 643 453
Bpu10I CC'TnA_GC
Cuts at: 3567 3840 4071 3567
Size: 273 231 7450
Fragments arranged by size:
7450 273 231
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2532 4923 6079 6944 7242 2532
Size: 2391 1156 865 298 3244
Fragments arranged by size:
3244 2391 1156 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6617 882
Size: 617 5118 2219
Fragments arranged by size:
5118 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4171 5745 493
Size: 711 2967 1574 2702
Fragments arranged by size:
2967 2702 1574 711
BsaBI GATnn'nnATC
Cuts at: 3377 5067 7948 3377
Size: 1690 2881 3383
Fragments arranged by size:
3383 2881 1690
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2390 3068 4866 5761
Size: 53 83 186 936 857 678 1798 895
Cuts at: 5761 6143 6778 275
Size: 382 635 1451
Fragments arranged by size:
1798 1451 936 895 857 678 635 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 3383 4017 4186 6387 6785 7059 7206 7617 3383
Size: 634 169 2201 398 274 147 411 3720
Fragments arranged by size:
3720 2201 634 411 398 274 169 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3196 3226 3964 3994 4519
Size: 30 165 30 1793 30 738 30 525
Cuts at: 4519 4549 1178
Size: 30 4583
Fragments arranged by size:
4583 1793 738 525 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2288 3321 3376 4059 4725 4895 1186
Size: 1102 1033 55 683 666 170 4245
Fragments arranged by size:
4245 1102 1033 683 666 170 55
BseYI C'CCAG_C
Cuts at: 2182 3309 4260 7157 7653 2182
Size: 1127 951 2897 496 2483
Fragments arranged by size:
2897 2483 1127 951 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2336 2724 3738 3801 3968 4371 1163
Size: 573 600 388 1014 63 167 403 4746
Fragments arranged by size:
4746 1014 600 573 403 388 167 63
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5404 6165 7193 1540
Size: 275 186 3403 761 1028 2301
Fragments arranged by size:
3403 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2241 2899 4939 6015 6100 7171 2241
Size: 658 2040 1076 85 1071 3024
Fragments arranged by size:
3024 2040 1076 1071 658 85
BsmI GAATG_Cn'
Cuts at: 5465 5558 5465
Size: 93 7861
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2116 2241 2667 2899 3309 3995
Size: 399 222 104 125 426 232 410 686
Cuts at: 3995 4439 4939 5273 6015 6100 7171 1391
Size: 444 500 334 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 686 500 444 426 410
399 334 232 222 125 104 85
BspEI T'CCGG_A
Cuts at: 3383 4017 7617 3383
Size: 634 3600 3720
Fragments arranged by size:
3720 3600 634
BspHI T'CATG_A
Cuts at: 2616 4488 5843 7573 2616
Size: 1872 1355 1730 2997
Fragments arranged by size:
2997 1872 1730 1355
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2506 2613 4036 4133 5031 5309 844
Size: 1662 107 1423 97 898 278 3489
Fragments arranged by size:
3489 1662 1423 898 278 107 97
BsrBI CCG'CTC
Cuts at: 1394 1823 4349 5841 1394
Size: 429 2526 1492 3507
Fragments arranged by size:
3507 2526 1492 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2463 2659 4239 4377 4547 6449 6623
Size: 1183 1215 196 1580 138 170 1902 174
Cuts at: 6623 65
Size: 1396
Fragments arranged by size:
1902 1580 1396 1215 1183 196 174 170
138
BsrFI r'CCGG_y
Cuts at: 1700 1810 2030 3721 6598 6785 1700
Size: 110 220 1691 2877 187 2869
Fragments arranged by size:
2877 2869 1691 220 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7954
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7954
BssSI C'ACGA_G
Cuts at: 3018 6014 7026 3018
Size: 2996 1012 3946
Fragments arranged by size:
3946 2996 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2711 3665 794
Size: 1917 954 5083
Fragments arranged by size:
5083 1917 954
BstBI TT'CG_AA
Cuts at: 6758 6758
Size: 7954
BstEII G'GTnAC_C
Cuts at: 1184 2518 4128 1184
Size: 1334 1610 5010
Fragments arranged by size:
5010 1610 1334
BstXI CCAn_nnnn'nTGG
Cuts at: 2566 3409 3892 2566
Size: 843 483 6628
Fragments arranged by size:
6628 843 483
BstYI r'GATC_y
Cuts at: 1972 3191 3336 3684 5284 5651 6036 6053 7505
Size: 1219 145 348 1600 367 385 17 1452
Cuts at: 7505 7591 7603 7949 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1600 1452 1219 385 367 348 346
145 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2750 2750
Size: 7954
BtgI C'CryG_G
Cuts at: 513 1065 2004 2015 2529 3414 3942 4001 4200
Size: 552 939 11 514 885 528 59 199
Cuts at: 4200 513
Size: 4267
Fragments arranged by size:
4267 939 885 552 528 514 199 59
11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3645 3981 487
Size: 683 56 2419 336 4460
Fragments arranged by size:
4460 2419 683 336 56
BtsI GCAGTG_nn'
Cuts at: 2370 2403 3870 4692 5470 6264 6284 2370
Size: 33 1467 822 778 794 20 4040
Fragments arranged by size:
4040 1467 822 794 778 33 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7954
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2341 2376 3924 3959 529
Size: 35 1777 35 1548 35 4524
Fragments arranged by size:
4524 1777 1548 35 35 35
DraI TTT'AAA
Cuts at: 5258 5258
Size: 7954
DraIII CAC_nnn'GTG
Cuts at: 4595 4595
Size: 7954
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4871 6961 809
Size: 507 3555 2090 1802
Fragments arranged by size:
3555 2090 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2831 3411 3894
Size: 54 1425 50 275 18 1001 580 483
Cuts at: 3894 4004 4179 5401 6290 7919 8
Size: 110 175 1222 889 1629 43
Fragments arranged by size:
1629 1425 1222 1001 889 580 483 275
175 110 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5401 1537
Size: 275 3589 4090
Fragments arranged by size:
4090 3589 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2676 5884 1353
Size: 1323 3208 3423
Fragments arranged by size:
3423 3208 1323
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6541 6915 7061 387
Size: 1064 138 4952 374 146 1280
Fragments arranged by size:
4952 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2897 2897
Size: 7954
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2241 2246 2538 2889 3094 3159 3794
Size: 393 609 5 292 351 205 65 635
Cuts at: 3794 3843 3867 3912 4365 5210 6017 6614 7401
Size: 49 24 45 453 845 807 597 787
Cuts at: 7401 7669 1239
Size: 268 1524
Fragments arranged by size:
1524 845 807 787 635 609 597 453
393 351 292 268 205 65 49 45
24 5
EcoNI CCTnn'n_nnAGG
Cuts at: 2106 3060 4603 2106
Size: 954 1543 5457
Fragments arranged by size:
5457 1543 954
EcoO109I rG'GnC_Cy
Cuts at: 2009 2137 2301 2664 3735 4436 2009
Size: 128 164 363 1071 701 5527
Fragments arranged by size:
5527 1071 701 363 164 128
EcoRI G'AATT_C
Cuts at: 3774 5263 5641 3774
Size: 1489 378 6087
Fragments arranged by size:
6087 1489 378
EcoRV GAT'ATC
Cuts at: 4486 4486
Size: 7954
FspI TGC'GCA
Cuts at: 6460 6460
Size: 7954
HaeII r_GCGC'y
Cuts at: 1678 7101 1678
Size: 5423 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4196 4228 4673 4705 6595
Size: 32 135 32 2624 32 445 32 1890
Cuts at: 6595 6627 6669 6701 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2624 1890 445 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2328 5298 5544 669
Size: 988 281 390 2970 246 3079
Fragments arranged by size:
3079 2970 988 390 281 246
HindIII A'AGCT_T
Cuts at: 748 2550 748
Size: 1802 6152
HpaI GTT'AAC
Cuts at: 5544 5544
Size: 7954
KpnI G_GTAC'C
Cuts at: 5279 5279
Size: 7954
MfeI C'AATT_G
Cuts at: 3888 5553 3888
Size: 1665 6289
MluI A'CGCG_T
Cuts at: 7830 7830
Size: 7954
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3571 4277 4307 7068 7252 1305
Size: 2266 706 30 2761 184 2007
Fragments arranged by size:
2761 2266 2007 706 184 30
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3413 3896 4181 10
Size: 54 1425 343 1581 483 285 3783
Fragments arranged by size:
3783 1581 1425 483 343 285 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2564 3125 3890 4959 5912 6271 6430
Size: 648 1398 561 765 1069 953 359 159
Cuts at: 6430 518
Size: 2042
Fragments arranged by size:
2042 1398 1069 953 765 648 561 359
159
MspA1I CmG'CkG
Cuts at: 1606 2017 2186 2708 2767 2924 3188 4385 4981
Size: 411 169 522 59 157 264 1197 596
Cuts at: 4981 5370 6047 7195 7440 1606
Size: 389 677 1148 245 2120
Fragments arranged by size:
2120 1197 1148 677 596 522 411 389
264 245 169 157 59
NaeI GCC'GGC
Cuts at: 2032 3723 2032
Size: 1691 6263
NcoI C'CATG_G
Cuts at: 513 1065 2004 2529 3414 4200 513
Size: 552 939 525 885 786 4267
Fragments arranged by size:
4267 939 885 786 552 525
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7954
NgoMIV G'CCGG_C
Cuts at: 2030 3721 2030
Size: 1691 6263
NheI G'CTAG_C
Cuts at: 1052 3968 1052
Size: 2916 5038
NotI GC'GGCC_GC
Cuts at: 5401 5401
Size: 7954
NsiI A_TGCA'T
Cuts at: 3661 3661
Size: 7954
NspI r_CATG'y
Cuts at: 1132 1229 2933 3668 4634 5312 6857 1132
Size: 97 1704 735 966 678 1545 2229
Fragments arranged by size:
2229 1704 1545 966 735 678 97
PasI CC'CwG_GG
Cuts at: 1348 3709 3740 3988 1348
Size: 2361 31 248 5314
Fragments arranged by size:
5314 2361 248 31
PciI A'CATG_T
Cuts at: 6853 6853
Size: 7954
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7954
PfoI T'CCnGG_A
Cuts at: 4342 4342
Size: 7954
PmeI GTTT'AAAC
Cuts at: 5258 5258
Size: 7954
PmlI CAC'GTG
Cuts at: 4171 4171
Size: 7954
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6023 6055 7536 7568 6023
Size: 32 1481 32 6409
Fragments arranged by size:
6409 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3735 3735
Size: 7954
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7954
PsiI TTA'TAA
Cuts at: 5524 5524
Size: 7954
PspOMI G'GGCC_C
Cuts at: 2008 2663 4435 2008
Size: 655 1772 5527
Fragments arranged by size:
5527 1772 655
PspXI vC'TCGA_Gb
Cuts at: 2697 4095 2697
Size: 1398 6556
PstI C_TGCA'G
Cuts at: 830 2989 3136 4144 4507 4902 5028 5306 830
Size: 2159 147 1008 363 395 126 278 3478
Fragments arranged by size:
3478 2159 1008 395 363 278 147 126
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7954
PvuII CAG'CTG
Cuts at: 2186 2708 2767 3188 4385 4981 2186
Size: 522 59 421 1197 596 5159
Fragments arranged by size:
5159 1197 596 522 421 59
SacI G_AGCT'C
Cuts at: 2899 2899
Size: 7954
SacII CC_GC'GG
Cuts at: 2018 2018
Size: 7954
SalI G'TCGA_C
Cuts at: 1936 2326 5296 1936
Size: 390 2970 4594
Fragments arranged by size:
4594 2970 390
SanDI GG'GwC_CC
Cuts at: 3735 3735
Size: 7954
SbfI CC_TGCA'GG
Cuts at: 4144 5028 5306 4144
Size: 884 278 6792
Fragments arranged by size:
6792 884 278
ScaI AGT'ACT
Cuts at: 3115 6202 7914 3115
Size: 3087 1712 3155
Fragments arranged by size:
3155 3087 1712
SexAI A'CCwGG_T
Cuts at: 4591 4591
Size: 7954
SfcI C'TryA_G
Cuts at: 826 1045 2072 2190 2985 3132 4140 4503 4898
Size: 219 1027 118 795 147 1008 363 395
Cuts at: 4898 4976 5024 5302 6437 7118 7309 826
Size: 78 48 278 1135 681 191 1471
Fragments arranged by size:
1471 1135 1027 1008 795 681 395 363
278 219 191 147 118 78 48
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7954
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7954
SmaI CCC'GGG
Cuts at: 1872 2270 5281 1872
Size: 398 3011 4545
Fragments arranged by size:
4545 3011 398
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2547 2697 4095 4938 5159 6058
Size: 197 926 604 150 1398 843 221 899
Cuts at: 6058 6959 7221 820
Size: 901 262 1553
Fragments arranged by size:
1553 1398 926 901 899 843 604 262
221 197 150
SnaBI TAC'GTA
Cuts at: 493 5745 493
Size: 5252 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7954
SphI G_CATG'C
Cuts at: 1132 2933 3668 5312 1132
Size: 1801 735 1644 3774
Fragments arranged by size:
3774 1801 1644 735
SspI AAT'ATT
Cuts at: 5 52 3464 5878 5
Size: 47 3412 2414 2081
Fragments arranged by size:
3412 2414 2081 47
StuI AGG'CCT
Cuts at: 1517 4138 1517
Size: 2621 5333
StyI C'CwwG_G
Cuts at: 513 1065 2004 2502 2529 3297 3414 4133 4200
Size: 552 939 498 27 768 117 719 67
Cuts at: 4200 4604 4740 5393 513
Size: 404 136 653 3074
Fragments arranged by size:
3074 939 768 719 653 552 498 404
136 117 67 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3712 4381 4400 5981 5998 6151 6336
Size: 1255 2132 669 19 1581 17 153 185
Cuts at: 6336 6781 325
Size: 445 1498
Fragments arranged by size:
2132 1581 1498 1255 669 445 185 153
19 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2797 3113 6200
Size: 274 80 33 51 1443 820 316 3087
Cuts at: 6200 7912 96
Size: 1712 138
Fragments arranged by size:
3087 1712 1443 820 316 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2231 2263 3196 3228 3964 3996 4519 4551 7914
Size: 32 933 32 736 32 523 32 3363
Cuts at: 7914 7946 2231
Size: 32 2239
Fragments arranged by size:
3363 2239 933 736 523 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5183 1316
Size: 3867 4087
XbaI T'CTAG_A
Cuts at: 4990 5290 4990
Size: 300 7654
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4039 4705 4039
Size: 666 7288
XhoI C'TCGA_G
Cuts at: 1943 2697 4095 1943
Size: 754 1398 5802
Fragments arranged by size:
5802 1398 754
XmaI C'CCGG_G
Cuts at: 1870 2268 5279 1870
Size: 398 3011 4545
Fragments arranged by size:
4545 3011 398
XmnI GAAnn'nnTTC
Cuts at: 3856 6083 3856
Size: 2227 5727
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4867 5762 276
Size: 53 83 186 936 3333 895 2468
Fragments arranged by size:
3333 2468 936 895 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacI SacII SalI SanDI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BbeI BplI BsiWI BsmBI BstZ17I
FalI FseI FspAI KasI NarI NruI PacI PsrI
RsrII SapI SfiI SfoI SrfI SwaI