Restriction map for
pFN21AB6003
(Circular) (Six-base) MAPSORT of: pFN21AB6003.seq Check: 3953 from: 1 to: 8299
pFN21AB6003 8299 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2152 2152
Size: 8299
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5216 6109 278
Size: 53 83 186 936 3680 893 2468
Fragments arranged by size:
3680 2468 936 893 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2512 5457 5642 1937
Size: 575 2945 185 4594
Fragments arranged by size:
4594 2945 575 185
Acc65I G'GTAC_C
Cuts at: 5620 5620
Size: 8299
AclI AA'CG_TT
Cuts at: 1563 2814 6426 6799 1563
Size: 1251 3612 373 3063
Fragments arranged by size:
3612 3063 1251 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2448 3658 3914 4130 4211 4356 4625 5501
Size: 816 1210 256 216 81 145 269 876
Cuts at: 5501 5597 6362 7746 8014 1632
Size: 96 765 1384 268 1917
Fragments arranged by size:
1917 1384 1210 876 816 765 269 268
256 216 145 96 81
AflII C'TTAA_G
Cuts at: 820 1017 3175 820
Size: 197 2158 5944
Fragments arranged by size:
5944 2158 197
AflIII A'CryG_T
Cuts at: 1203 4705 7198 8175 1203
Size: 3502 2493 977 1327
Fragments arranged by size:
3502 2493 1327 977
AgeI A'CCGG_T
Cuts at: 7130 7130
Size: 8299
AhdI GACnn_n'nnGTC
Cuts at: 7028 7028
Size: 8299
AleI CACnn'nnGTG
Cuts at: 1166 5491 1166
Size: 4325 3974
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5299 1184
Size: 4115 4184
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5198 5230 5198
Size: 32 8267
AlwNI CAG_nnn'CTG
Cuts at: 4324 4864 5299 6042 7614 4324
Size: 540 435 743 1572 5009
Fragments arranged by size:
5009 1572 743 540 435
ApaLI G'TGCA_C
Cuts at: 4554 6356 7512 4554
Size: 1802 1156 5341
Fragments arranged by size:
5341 1802 1156
ApoI r'AATT_y
Cuts at: 1493 2274 2490 3765 3943 4390 4468 4517 5474
Size: 781 216 1275 178 447 78 49 957
Cuts at: 5474 5608 5834 5986 8095 1493
Size: 134 226 152 2109 1697
Fragments arranged by size:
2109 1697 1275 957 781 447 226 216
178 152 134 78 49
AseI AT'TA_AT
Cuts at: 160 1032 2210 3783 3837 6853 160
Size: 872 1178 1573 54 3016 1606
Fragments arranged by size:
3016 1606 1573 1178 872 54
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8299
AvaI C'yCGr_G
Cuts at: 1870 1943 5522 5624 1870
Size: 73 3579 102 4545
Fragments arranged by size:
4545 3579 102 73
BamHI G'GATC_C
Cuts at: 3600 5629 5996 3600
Size: 2029 367 5903
Fragments arranged by size:
5903 2029 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2359 5177 5620 7075 618
Size: 325 217 627 572 2818 443 1455 1842
Fragments arranged by size:
2818 1842 1455 627 572 443 325 217
BanII G_rGCy'C
Cuts at: 1391 4347 5618 1391
Size: 2956 1271 4072
Fragments arranged by size:
4072 2956 1271
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2340 2589 3417 3920 928
Size: 971 441 249 828 503 5307
Fragments arranged by size:
5307 971 828 503 441 249
BbvCI CC'TCA_GC
Cuts at: 2072 2072
Size: 8299
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6490 6524 1903
Size: 34 4553 34 3678
Fragments arranged by size:
4553 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6191 7401 6191
Size: 1210 7089
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8299
BfrBI ATG'CAT
Cuts at: 3476 5047 3476
Size: 1571 6728
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5402 6910
Size: 107 122 71 631 605 337 3393 1508
Cuts at: 6910 136
Size: 1525
Fragments arranged by size:
3393 1525 1508 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 3062 8294 3062
Size: 5232 3067
BlpI GC'TnA_GC
Cuts at: 3054 5716 3054
Size: 2662 5637
Bme1580I G_kGCm'C
Cuts at: 1790 2364 4129 4558 5334 6360 7516 1790
Size: 574 1765 429 776 1026 1156 2573
Fragments arranged by size:
2573 1765 1156 1026 776 574 429
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8299
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2137 6030 6988
Size: 416 53 83 399 24 720 3893 958
Cuts at: 6988 442
Size: 1753
Fragments arranged by size:
3893 1753 958 720 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8299
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2445 2731 3053 3056 3343 3910 5212 5269
Size: 1206 286 322 3 287 567 1302 57
Cuts at: 5269 6959 1239
Size: 1690 2579
Fragments arranged by size:
2579 1690 1302 1206 567 322 287 286
57 3
Bpu10I CC'TnA_GC
Cuts at: 2072 2072
Size: 8299
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2551 4151 6424 7289 7587 2551
Size: 1600 2273 865 298 3263
Fragments arranged by size:
3263 2273 1600 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6962 882
Size: 617 5463 2219
Fragments arranged by size:
5463 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5461 6090 493
Size: 711 4257 629 2702
Fragments arranged by size:
4257 2702 711 629
BsaBI GATnn'nnATC
Cuts at: 8293 8293
Size: 8299
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4576 5213 5382 6106
Size: 53 83 186 936 3043 637 169 724
Cuts at: 6106 6488 7123 275
Size: 382 635 1451
Fragments arranged by size:
3043 1451 936 724 637 635 382 186
169 83 53
BsaWI w'CCGG_w
Cuts at: 3580 6732 7130 7404 7551 7962 3580
Size: 3152 398 274 147 411 3917
Fragments arranged by size:
3917 3152 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2434 2464 5096 5126 1178
Size: 30 165 30 1031 30 2632 30 4351
Fragments arranged by size:
4351 2632 1031 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2060 3179 4379 4454 1186
Size: 874 1119 1200 75 5031
Fragments arranged by size:
5031 1200 1119 874 75
BseYI C'CCAG_C
Cuts at: 4874 4902 5533 7502 7998 4874
Size: 28 631 1969 496 5175
Fragments arranged by size:
5175 1969 631 496 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2547 5075 5285 1163
Size: 573 811 2528 210 4177
Fragments arranged by size:
4177 2528 811 573 210
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5749 6510 7538 1540
Size: 275 186 3748 761 1028 2301
Fragments arranged by size:
3748 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2761 2830 2952 4558 6360 6445 7516 2761
Size: 69 122 1606 1802 85 1071 3544
Fragments arranged by size:
3544 1802 1606 1071 122 85 69
BsmI GAATG_Cn'
Cuts at: 4892 5810 5903 4892
Size: 918 93 7288
Fragments arranged by size:
7288 918 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2364 2761 2830 2952 4129 4347 4558
Size: 399 574 397 69 122 1177 218 211
Cuts at: 4558 5334 5618 6360 6445 7516 1391
Size: 776 284 742 85 1071 2174
Fragments arranged by size:
2174 1177 1071 776 742 574 399 397
284 218 211 122 85 69
BspEI T'CCGG_A
Cuts at: 3580 7962 3580
Size: 4382 3917
BspHI T'CATG_A
Cuts at: 2194 2650 3190 4264 6188 7918 2194
Size: 456 540 1074 1924 1730 2575
Fragments arranged by size:
2575 1924 1730 1074 540 456
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2152 5654 844
Size: 1308 3502 3489
Fragments arranged by size:
3502 3489 1308
BsrBI CCG'CTC
Cuts at: 1394 1823 6186 1394
Size: 429 4363 3507
Fragments arranged by size:
4363 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2790 2928 3268 4453 5013 5029 5458
Size: 1183 1542 138 340 1185 560 16 429
Cuts at: 5458 5472 6794 6968 65
Size: 14 1322 174 1396
Fragments arranged by size:
1542 1396 1322 1185 1183 560 429 340
174 138 16 14
BsrFI r'CCGG_y
Cuts at: 1700 1810 2031 6943 7130 1700
Size: 110 221 4912 187 2869
Fragments arranged by size:
4912 2869 221 187 110
BsrGI T'GTAC_A
Cuts at: 96 2539 3521 4227 96
Size: 2443 982 706 4168
Fragments arranged by size:
4168 2443 982 706
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8299
BssSI C'ACGA_G
Cuts at: 2103 2677 5038 6359 7371 2103
Size: 574 2361 1321 1012 3031
Fragments arranged by size:
3031 2361 1321 1012 574
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8299
BstBI TT'CG_AA
Cuts at: 7103 7103
Size: 8299
BstEII G'GTnAC_C
Cuts at: 1184 2639 1184
Size: 1455 6844
BstXI CCAn_nnnn'nTGG
Cuts at: 5066 5197 5066
Size: 131 8168
BstYI r'GATC_y
Cuts at: 1972 2377 3062 3600 3727 5629 5996 6381 6398
Size: 405 685 538 127 1902 367 385 17
Cuts at: 6398 7850 7936 7948 8294 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1902 1452 685 538 405 385 367
346 127 86 17 12
BstZ17I GTA'TAC
Cuts at: 2513 2513
Size: 8299
Bsu36I CC'TnA_GG
Cuts at: 2365 2365
Size: 8299
BtgI C'CryG_G
Cuts at: 513 1065 2004 5400 513
Size: 552 939 3396 3412
Fragments arranged by size:
3412 3396 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7560
Fragments arranged by size:
7560 683 56
BtsI GCAGTG_nn'
Cuts at: 3622 4258 5815 6609 6629 3622
Size: 636 1557 794 20 5292
Fragments arranged by size:
5292 1557 794 636 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8299
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8264
DraI TTT'AAA
Cuts at: 5603 5603
Size: 8299
DraIII CAC_nnn'GTG
Cuts at: 2947 2947
Size: 8299
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7306 809
Size: 507 5990 1802
Fragments arranged by size:
5990 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2029 3987 4050
Size: 54 1425 50 275 18 199 1958 63
Cuts at: 4050 4629 5346 5746 6635 8264 8
Size: 579 717 400 889 1629 43
Fragments arranged by size:
1958 1629 1425 889 717 579 400 275
199 63 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5746 1537
Size: 275 3934 4090
Fragments arranged by size:
4090 3934 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2280 2776 3633 4335 6229 1353
Size: 927 496 857 702 1894 3423
Fragments arranged by size:
3423 1894 927 857 702 496
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2071 5279 6886 7260 7406 387
Size: 1064 138 482 3208 1607 374 146 1280
Fragments arranged by size:
3208 1607 1280 1064 482 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2445 2448 2731 3053 3056 3343 3658
Size: 393 813 3 283 322 3 287 315
Cuts at: 3658 3910 3914 4130 4211 4356 4625 5212 5269
Size: 252 4 216 81 145 269 587 57
Cuts at: 5269 5501 5597 6362 6959 7746 8014 1239
Size: 232 96 765 597 787 268 1524
Fragments arranged by size:
1524 813 787 765 597 587 393 322
315 287 283 269 268 252 232 216
145 96 81 57 4 3 3
EcoNI CCTnn'n_nnAGG
Cuts at: 4539 5291 4539
Size: 752 7547
EcoO109I rG'GnC_Cy
Cuts at: 2109 2371 4369 4613 5221 2109
Size: 262 1998 244 608 5187
Fragments arranged by size:
5187 1998 608 262 244
EcoRI G'AATT_C
Cuts at: 4390 4468 5608 5986 4390
Size: 78 1140 378 6703
Fragments arranged by size:
6703 1140 378 78
EcoRV GAT'ATC
Cuts at: 3310 3406 3310
Size: 96 8203
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2380 2380
Size: 8299
FspI TGC'GCA
Cuts at: 6805 6805
Size: 8299
HaeII r_GCGC'y
Cuts at: 1678 5593 7446 1678
Size: 3915 1853 2531
Fragments arranged by size:
3915 2531 1853
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2432 2464 2539 2571 3335
Size: 32 135 32 860 32 75 32 764
Cuts at: 3335 3367 3374 3406 3585 3617 6940 6972 7014
Size: 32 7 32 179 32 3323 32 42
Cuts at: 7014 7046 1373
Size: 32 2626
Fragments arranged by size:
3323 2626 860 764 179 135 75 42
32 32 32 32 32 32 32 32
32 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2233 2803 3250 3982 5115 5643
Size: 988 281 295 570 447 732 1133 528
Cuts at: 5643 5889 669
Size: 246 3079
Fragments arranged by size:
3079 1133 988 732 570 528 447 295
281 246
HindIII A'AGCT_T
Cuts at: 748 4044 5513 748
Size: 3296 1469 3534
Fragments arranged by size:
3534 3296 1469
HpaI GTT'AAC
Cuts at: 2233 5115 5889 2233
Size: 2882 774 4643
Fragments arranged by size:
4643 2882 774
KpnI G_GTAC'C
Cuts at: 5624 5624
Size: 8299
MfeI C'AATT_G
Cuts at: 5898 5898
Size: 8299
MluI A'CGCG_T
Cuts at: 8175 8175
Size: 8299
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5100 7413 7597 1305
Size: 3795 2313 184 2007
Fragments arranged by size:
3795 2313 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3989 4052 4631 5348 10
Size: 54 1425 343 2157 63 579 717 2961
Fragments arranged by size:
2961 2157 1425 717 579 343 63 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2193 2210 4020 5042 5064 5491 6257
Size: 648 1027 17 1810 1022 22 427 766
Cuts at: 6257 6616 6775 518
Size: 359 159 2042
Fragments arranged by size:
2042 1810 1027 1022 766 648 427 359
159 22 17
MspA1I CmG'CkG
Cuts at: 1606 4203 4902 5715 6392 7540 7785 1606
Size: 2597 699 813 677 1148 245 2120
Fragments arranged by size:
2597 2120 1148 813 699 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 5400 513
Size: 552 939 3396 3412
Fragments arranged by size:
3412 3396 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8299
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8299
NotI GC'GGCC_GC
Cuts at: 5746 5746
Size: 8299
NsiI A_TGCA'T
Cuts at: 3478 5049 3478
Size: 1571 6728
NspI r_CATG'y
Cuts at: 1132 1229 2519 3014 5657 7202 1132
Size: 97 1290 495 2643 1545 2229
Fragments arranged by size:
2643 2229 1545 1290 495 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8299
PciI A'CATG_T
Cuts at: 7198 7198
Size: 8299
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2127 4864 5373 1108
Size: 1019 2737 509 4034
Fragments arranged by size:
4034 2737 1019 509
PmeI GTTT'AAAC
Cuts at: 5603 5603
Size: 8299
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2660 2692 4435 4467 6368 6400 7881 7913 2660
Size: 32 1743 32 1901 32 1481 32 3046
Fragments arranged by size:
3046 1901 1743 1481 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2371 4369 4613 5221 2371
Size: 1998 244 608 5449
Fragments arranged by size:
5449 1998 608 244
PshAI GACnn'nnGTC
Cuts at: 1613 2421 2637 1613
Size: 808 216 7275
Fragments arranged by size:
7275 808 216
PsiI TTA'TAA
Cuts at: 5869 5869
Size: 8299
PstI C_TGCA'G
Cuts at: 830 4658 5651 830
Size: 3828 993 3478
Fragments arranged by size:
3828 3478 993
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8299
SalI G'TCGA_C
Cuts at: 1936 5641 1936
Size: 3705 4594
SapI GCTCTTCn'nnn_
Cuts at: 4335 4335
Size: 8299
SbfI CC_TGCA'GG
Cuts at: 5651 5651
Size: 8299
ScaI AGT'ACT
Cuts at: 6547 8259 6547
Size: 1712 6587
SfcI C'TryA_G
Cuts at: 826 1045 2660 3630 4654 5647 6782 7463 7654
Size: 219 1615 970 1024 993 1135 681 191
Cuts at: 7654 826
Size: 1471
Fragments arranged by size:
1615 1471 1135 1024 993 970 681 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8299
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8299
SmaI CCC'GGG
Cuts at: 1872 5626 1872
Size: 3754 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2530 3175 4130 6403 7304 7566
Size: 197 926 587 645 955 2273 901 262
Cuts at: 7566 820
Size: 1553
Fragments arranged by size:
2273 1553 955 926 901 645 587 262
197
SnaBI TAC'GTA
Cuts at: 493 6090 493
Size: 5597 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8299
SphI G_CATG'C
Cuts at: 1132 3014 5657 1132
Size: 1882 2643 3774
Fragments arranged by size:
3774 2643 1882
SspI AAT'ATT
Cuts at: 5 52 4304 6223 5
Size: 47 4252 1919 2081
Fragments arranged by size:
4252 2081 1919 47
StuI AGG'CCT
Cuts at: 1517 2658 4136 4148 1517
Size: 1141 1478 12 5668
Fragments arranged by size:
5668 1478 1141 12
StyI C'CwwG_G
Cuts at: 513 1065 2004 2355 4815 5263 5400 5738 513
Size: 552 939 351 2460 448 137 338 3074
Fragments arranged by size:
3074 2460 939 552 448 351 338 137
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3406 6326 6343 6496 6681 7126 325
Size: 1255 1826 2920 17 153 185 445 1498
Fragments arranged by size:
2920 1826 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2483 2539 3521
Size: 274 80 33 51 1443 506 56 982
Cuts at: 3521 4227 6545 8257 96
Size: 706 2318 1712 138
Fragments arranged by size:
2318 1712 1443 982 706 506 274 138
80 56 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2434 2466 2756 2788 3677 3709 4153 4185 4584
Size: 32 290 32 889 32 444 32 399
Cuts at: 4584 4616 8259 8291 2434
Size: 32 3643 32 2442
Fragments arranged by size:
3643 2442 889 444 399 290 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2201 5384 1316
Size: 885 3183 4231
Fragments arranged by size:
4231 3183 885
XbaI T'CTAG_A
Cuts at: 2494 4579 5635 2494
Size: 2085 1056 5158
Fragments arranged by size:
5158 2085 1056
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8299
XmaI C'CCGG_G
Cuts at: 1870 5624 1870
Size: 3754 4545
XmnI GAAnn'nnTTC
Cuts at: 5438 6428 5438
Size: 990 7309
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5214 6107 276
Size: 53 83 186 936 3680 893 2468
Fragments arranged by size:
3680 2468 936 893 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BplI BsiWI
BsmBI EcoICRI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI