Restriction map for
pFN21AB6002
(Circular) (Six-base) MAPSORT of: pFN21AB6002.seq Check: 51 from: 1 to: 9763
pFN21AB6002 9763 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2617 2617
Size: 9763
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7573 278
Size: 53 83 186 936 6037 2468
Fragments arranged by size:
6037 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6888 7106 1937
Size: 4951 218 4594
Fragments arranged by size:
4951 4594 218
Acc65I G'GTAC_C
Cuts at: 7084 7084
Size: 9763
AclI AA'CG_TT
Cuts at: 1563 4699 7890 8263 1563
Size: 3136 3191 373 3063
Fragments arranged by size:
3191 3136 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3517 5323 5673 5688 6006 6643 6645 6783
Size: 1885 1806 350 15 318 637 2 138
Cuts at: 6783 7826 9210 9478 1632
Size: 1043 1384 268 1917
Fragments arranged by size:
1917 1885 1806 1384 1043 637 350 318
268 138 15 2
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9566
AflIII A'CryG_T
Cuts at: 1203 3323 4412 5089 5793 8662 9639 1203
Size: 2120 1089 677 704 2869 977 1327
Fragments arranged by size:
2869 2120 1327 1089 977 704 677
AgeI A'CCGG_T
Cuts at: 8594 8594
Size: 9763
AhdI GACnn_n'nnGTC
Cuts at: 8492 8492
Size: 9763
AleI CACnn'nnGTG
Cuts at: 1166 5372 1166
Size: 4206 5557
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9763
AlwNI CAG_nnn'CTG
Cuts at: 7506 9078 7506
Size: 1572 8191
ApaLI G'TGCA_C
Cuts at: 7820 8976 7820
Size: 1156 8607
ApoI r'AATT_y
Cuts at: 1493 2708 2804 3185 3985 4089 4204 4302 4456
Size: 1215 96 381 800 104 115 98 154
Cuts at: 4456 4850 4959 6345 6398 6843 6967 7072 7298
Size: 394 109 1386 53 445 124 105 226
Cuts at: 7298 7450 9559 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1386 1215 800 445 394 381
226 154 152 124 115 109 105 104
98 96 53
AseI AT'TA_AT
Cuts at: 160 1032 5403 5901 8317 160
Size: 872 4371 498 2416 1606
Fragments arranged by size:
4371 2416 1606 872 498
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9763
AvaI C'yCGr_G
Cuts at: 1870 1943 7088 1870
Size: 73 5145 4545
Fragments arranged by size:
5145 4545 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3590 3623 3590
Size: 33 9730
BamHI G'GATC_C
Cuts at: 2824 2908 5615 7093 7460 2824
Size: 84 2707 1478 367 5127
Fragments arranged by size:
5127 2707 1478 367 84
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 7084 8539 618
Size: 325 217 627 5297 1455 1842
Fragments arranged by size:
5297 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2378 3905 5866 6225 7082 1391
Size: 987 1527 1961 359 857 4072
Fragments arranged by size:
4072 1961 1527 987 857 359
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2561 3185 3882 4721 6197 6373 928
Size: 971 662 624 697 839 1476 176 4318
Fragments arranged by size:
4318 1476 971 839 697 662 624 176
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3767 3801 7954 7988 1903
Size: 34 1830 34 4153 34 3678
Fragments arranged by size:
4153 3678 1830 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7655 8865 7655
Size: 1210 8553
BclI T'GATC_A
Cuts at: 1548 4858 1548
Size: 3310 6453
BfrBI ATG'CAT
Cuts at: 6742 6742
Size: 9763
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 8374 136
Size: 107 122 71 631 605 6702 1525
Fragments arranged by size:
6702 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4921 5428 5478 5500 6751 9758 4921
Size: 507 50 22 1251 3007 4926
Fragments arranged by size:
4926 3007 1251 507 50 22
BlpI GC'TnA_GC
Cuts at: 6186 7180 6186
Size: 994 8769
Bme1580I G_kGCm'C
Cuts at: 1790 4015 7824 8980 1790
Size: 2225 3809 1156 2573
Fragments arranged by size:
3809 2573 2225 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9763
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 7494 8452 442
Size: 416 53 83 399 24 6077 958 1753
Fragments arranged by size:
6077 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2903 1056
Size: 1847 7916
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4046 6654 8423 1239
Size: 2807 2608 1769 2579
Fragments arranged by size:
2807 2608 2579 1769
Bpu10I CC'TnA_GC
Cuts at: 5648 5648
Size: 9763
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2324 4545 5273 7033 7888 8753 9051 2324
Size: 2221 728 1760 855 865 298 3036
Fragments arranged by size:
3036 2221 1760 865 855 728 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3286 8426 882
Size: 617 1787 5140 2219
Fragments arranged by size:
5140 2219 1787 617
BsaAI yAC'GTr
Cuts at: 493 1204 3324 7554 493
Size: 711 2120 4230 2702
Fragments arranged by size:
4230 2702 2120 711
BsaBI GATnn'nnATC
Cuts at: 2223 3672 6743 9757 2223
Size: 1449 3071 3014 2229
Fragments arranged by size:
3071 3014 2229 1449
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 7570 7952 8587 275
Size: 53 83 186 936 6037 382 635 1451
Fragments arranged by size:
6037 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2056 8196 8594 8868 9015 9426 2056
Size: 6140 398 274 147 411 2393
Fragments arranged by size:
6140 2393 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 6675 6705 1178
Size: 30 165 30 5272 30 4236
Fragments arranged by size:
5272 4236 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3155 3885 4548 5190 6699 1186
Size: 1969 730 663 642 1509 4250
Fragments arranged by size:
4250 1969 1509 730 663 642
BseYI C'CCAG_C
Cuts at: 3372 8966 9462 3372
Size: 5594 496 3673
Fragments arranged by size:
5594 3673 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 6969 1163
Size: 573 5233 3957
Fragments arranged by size:
5233 3957 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7213 7974 9002 1540
Size: 275 186 5212 761 1028 2301
Fragments arranged by size:
5212 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5104 7824 7909 8980 5104
Size: 2720 85 1071 5887
Fragments arranged by size:
5887 2720 1071 85
BsmI GAATG_Cn'
Cuts at: 2504 2535 5383 6556 7274 7367 2504
Size: 31 2848 1173 718 93 4900
Fragments arranged by size:
4900 2848 1173 718 93 31
BsmBI CGTCTCn'nnnn_
Cuts at: 5285 7016 5285
Size: 1731 8032
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2378 3905 4015 5104 5866 6225 7082
Size: 399 588 1527 110 1089 762 359 857
Cuts at: 7082 7824 7909 8980 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1527 1089 1071 857 762 742 588
399 359 110 85
BspEI T'CCGG_A
Cuts at: 2056 9426 2056
Size: 7370 2393
BspHI T'CATG_A
Cuts at: 3472 7652 9382 3472
Size: 4180 1730 3853
Fragments arranged by size:
4180 3853 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2617 6641 7118 844
Size: 1773 4024 477 3489
Fragments arranged by size:
4024 3489 1773 477
BsrBI CCG'CTC
Cuts at: 1394 1823 7650 1394
Size: 429 5827 3507
Fragments arranged by size:
5827 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3457 3546 4790 6147 8258 8432 65
Size: 1183 2209 89 1244 1357 2111 174 1396
Fragments arranged by size:
2209 2111 1396 1357 1244 1183 174 89
BsrFI r'CCGG_y
Cuts at: 1700 1810 8407 8594 1700
Size: 110 6597 187 2869
Fragments arranged by size:
6597 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4939 5065 5161 96
Size: 4843 126 96 4698
Fragments arranged by size:
4843 4698 126 96
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9763
BssSI C'ACGA_G
Cuts at: 7823 8835 7823
Size: 1012 8751
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 9763
BstBI TT'CG_AA
Cuts at: 3028 5779 8567 3028
Size: 2751 2788 4224
Fragments arranged by size:
4224 2788 2751
BstEII G'GTnAC_C
Cuts at: 1184 6027 1184
Size: 4843 4920
BstXI CCAn_nnnn'nTGG
Cuts at: 5005 5005
Size: 9763
BstYI r'GATC_y
Cuts at: 1972 2824 2908 4159 4871 4921 5428 5478 5500
Size: 852 84 1251 712 50 507 50 22
Cuts at: 5500 5615 6751 7093 7460 7845 7862 9314 9400
Size: 115 1136 342 367 385 17 1452 86
Cuts at: 9400 9412 9758 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1251 1136 852 712 507 385
367 346 342 115 86 84 50 50
22 17 12
BstZ17I GTA'TAC
Cuts at: 6889 6889
Size: 9763
Bsu36I CC'TnA_GG
Cuts at: 2841 5582 2841
Size: 2741 7022
BtgI C'CryG_G
Cuts at: 513 1065 2004 3585 513
Size: 552 939 1581 6691
Fragments arranged by size:
6691 1581 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 9024
Fragments arranged by size:
9024 683 56
BtsI GCAGTG_nn'
Cuts at: 4324 7279 8073 8093 4324
Size: 2955 794 20 5994
Fragments arranged by size:
5994 2955 794 20
ClaI AT'CG_AT
Cuts at: 1554 2274 1554
Size: 720 9043
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2061 2096 5283 5318 529
Size: 35 1497 35 3187 35 4974
Fragments arranged by size:
4974 3187 1497 35 35 35
DraI TTT'AAA
Cuts at: 2035 2715 3021 3594 4168 4308 4399 5623 7067
Size: 680 306 573 574 140 91 1224 1444
Cuts at: 7067 2035
Size: 4731
Fragments arranged by size:
4731 1444 1224 680 574 573 306 140
91
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6267 8770 809
Size: 507 4951 2503 1802
Fragments arranged by size:
4951 2503 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2429 7210 8099
Size: 54 1425 50 275 18 599 4781 889
Cuts at: 8099 9728 8
Size: 1629 43
Fragments arranged by size:
4781 1629 1425 889 599 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7210 1537
Size: 275 5398 4090
Fragments arranged by size:
5398 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2871 2928 3117 3702 7693 1353
Size: 1518 57 189 585 3991 3423
Fragments arranged by size:
3991 3423 1518 585 189 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 8350 8724 8870 387
Size: 1064 138 6761 374 146 1280
Fragments arranged by size:
6761 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3517 4046 5323 5673 5688 6006 6643
Size: 393 1885 529 1277 350 15 318 637
Cuts at: 6643 6645 6654 6783 7826 8423 9210 9478 1239
Size: 2 9 129 1043 597 787 268 1524
Fragments arranged by size:
1885 1524 1277 1043 787 637 597 529
393 350 318 268 129 15 9 2
EcoNI CCTnn'n_nnAGG
Cuts at: 6669 6669
Size: 9763
EcoRI G'AATT_C
Cuts at: 7072 7450 7072
Size: 378 9385
EcoRV GAT'ATC
Cuts at: 2016 2272 2797 4257 2016
Size: 256 525 1460 7522
Fragments arranged by size:
7522 1460 525 256
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2318 3033 4225 4643 6181 2318
Size: 715 1192 418 1538 5900
Fragments arranged by size:
5900 1538 1192 715 418
FspI TGC'GCA
Cuts at: 8269 8269
Size: 9763
HaeII r_GCGC'y
Cuts at: 1678 4752 8910 1678
Size: 3074 4158 2531
Fragments arranged by size:
4158 3074 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2140 2172 3507 3539 8404
Size: 32 135 32 568 32 1335 32 4865
Cuts at: 8404 8436 8478 8510 1373
Size: 32 42 32 2626
Fragments arranged by size:
4865 2626 1335 568 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4567 7107 7353 669
Size: 988 281 2629 2540 246 3079
Fragments arranged by size:
3079 2629 2540 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2635 4362 4680 4944 748
Size: 1887 1727 318 264 5567
Fragments arranged by size:
5567 1887 1727 318 264
HpaI GTT'AAC
Cuts at: 7353 7353
Size: 9763
KpnI G_GTAC'C
Cuts at: 7088 7088
Size: 9763
MfeI C'AATT_G
Cuts at: 4262 7362 4262
Size: 3100 6663
MluI A'CGCG_T
Cuts at: 9639 9639
Size: 9763
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6890 8877 9061 1305
Size: 5585 1987 184 2007
Fragments arranged by size:
5585 2007 1987 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2431 10
Size: 54 1425 343 599 7342
Fragments arranged by size:
7342 1425 599 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3356 5372 6734 6737 7721 8080 8239
Size: 648 2190 2016 1362 3 984 359 159
Cuts at: 8239 518
Size: 2042
Fragments arranged by size:
2190 2042 2016 1362 984 648 359 159
3
MspA1I CmG'CkG
Cuts at: 1606 2028 7042 7179 7856 9004 9249 1606
Size: 422 5014 137 677 1148 245 2120
Fragments arranged by size:
5014 2120 1148 677 422 245 137
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 8272
Fragments arranged by size:
8272 939 552
NdeI CA'TA_TG
Cuts at: 387 3254 5893 6787 387
Size: 2867 2639 894 3363
Fragments arranged by size:
3363 2867 2639 894
NheI G'CTAG_C
Cuts at: 1052 2899 1052
Size: 1847 7916
NotI GC'GGCC_GC
Cuts at: 7210 7210
Size: 9763
NsiI A_TGCA'T
Cuts at: 6744 6744
Size: 9763
NspI r_CATG'y
Cuts at: 1132 1229 4416 5093 5797 5831 7121 8666 1132
Size: 97 3187 677 704 34 1290 1545 2229
Fragments arranged by size:
3187 2229 1545 1290 704 677 97 34
PasI CC'CwG_GG
Cuts at: 1348 3052 1348
Size: 1704 8059
PciI A'CATG_T
Cuts at: 4412 5089 5793 8662 4412
Size: 677 704 2869 5513
Fragments arranged by size:
5513 2869 704 677
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 9763
PfoI T'CCnGG_A
Cuts at: 2572 4465 2572
Size: 1893 7870
PmeI GTTT'AAAC
Cuts at: 7067 7067
Size: 9763
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7832 7864 9345 9377 7832
Size: 32 1481 32 8218
Fragments arranged by size:
8218 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9763
PsiI TTA'TAA
Cuts at: 2208 3356 7333 2208
Size: 1148 3977 4638
Fragments arranged by size:
4638 3977 1148
PstI C_TGCA'G
Cuts at: 830 3450 3955 6646 7115 830
Size: 2620 505 2691 469 3478
Fragments arranged by size:
3478 2691 2620 505 469
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9763
PvuII CAG'CTG
Cuts at: 7042 7042
Size: 9763
SalI G'TCGA_C
Cuts at: 1936 7105 1936
Size: 5169 4594
SapI GCTCTTCn'nnn_
Cuts at: 3702 3702
Size: 9763
SbfI CC_TGCA'GG
Cuts at: 7115 7115
Size: 9763
ScaI AGT'ACT
Cuts at: 2665 8011 9723 2665
Size: 5346 1712 2705
Fragments arranged by size:
5346 2705 1712
SfcI C'TryA_G
Cuts at: 826 1045 2547 2771 3342 3446 3757 3951 4002
Size: 219 1502 224 571 104 311 194 51
Cuts at: 4002 4511 6090 6642 7111 8246 8927 9118 826
Size: 509 1579 552 469 1135 681 191 1471
Fragments arranged by size:
1579 1502 1471 1135 681 571 552 509
469 311 224 219 194 191 104 51
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9763
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9763
SmaI CCC'GGG
Cuts at: 1872 7090 1872
Size: 5218 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2303 4560 5288 7012 7867 8768
Size: 197 926 360 2257 728 1724 855 901
Cuts at: 8768 9030 820
Size: 262 1553
Fragments arranged by size:
2257 1724 1553 926 901 855 728 360
262 197
SnaBI TAC'GTA
Cuts at: 493 7554 493
Size: 7061 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9763
SphI G_CATG'C
Cuts at: 1132 7121 1132
Size: 5989 3774
SspI AAT'ATT
Cuts at: 5 52 3025 4740 5320 7687 5
Size: 47 2973 1715 580 2367 2081
Fragments arranged by size:
2973 2367 2081 1715 580 47
StuI AGG'CCT
Cuts at: 1517 5960 1517
Size: 4443 5320
StyI C'CwwG_G
Cuts at: 513 1065 2004 2912 3463 3833 7202 513
Size: 552 939 908 551 370 3369 3074
Fragments arranged by size:
3369 3074 939 908 552 551 370
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2306 4734 5087 6968 7790 7807 7960
Size: 1255 726 2428 353 1881 822 17 153
Cuts at: 7960 8145 8590 325
Size: 185 445 1498
Fragments arranged by size:
2428 1881 1498 1255 822 726 445 353
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2663 2699 3130
Size: 274 80 33 51 1443 686 36 431
Cuts at: 3130 4939 5065 5161 5248 6587 6940 8009 9721
Size: 1809 126 96 87 1339 353 1069 1712
Cuts at: 9721 96
Size: 138
Fragments arranged by size:
1809 1712 1443 1339 1069 686 431 353
274 138 126 96 87 80 51 36
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6675 6707 6809 6841 9723 9755 6675
Size: 32 102 32 2882 32 6683
Fragments arranged by size:
6683 2882 102 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9763
XbaI T'CTAG_A
Cuts at: 7099 7099
Size: 9763
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3986 4761 5600 5640 3986
Size: 775 839 40 8109
Fragments arranged by size:
8109 839 775 40
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9763
XmaI C'CCGG_G
Cuts at: 1870 7088 1870
Size: 5218 4545
XmnI GAAnn'nnTTC
Cuts at: 3300 4208 4278 5139 5430 6971 7004 7892 3300
Size: 908 70 861 291 1541 33 888 5171
Fragments arranged by size:
5171 1541 908 888 861 291 70 33
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7571 276
Size: 53 83 186 936 6037 2468
Fragments arranged by size:
6037 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BbvCI BplI
BsiWI DraIII EcoICRI EcoO109I FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI