Restriction map for
pFN21AB5996
(Circular) (Six-base) MAPSORT of: pFN21AB5996.seq Check: 9981 from: 1 to: 6544
pFN21AB5996 6544 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4354 278
Size: 53 83 186 936 2818 2468
Fragments arranged by size:
2818 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3887 1937
Size: 1950 4594
Acc65I G'GTAC_C
Cuts at: 3865 3865
Size: 6544
AclI AA'CG_TT
Cuts at: 1563 4671 5044 1563
Size: 3108 373 3063
Fragments arranged by size:
3108 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2784 3116 4607 5991 6259 1632
Size: 1152 332 1491 1384 268 1917
Fragments arranged by size:
1917 1491 1384 1152 332 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6347
AflIII A'CryG_T
Cuts at: 1203 2156 3696 5443 6420 1203
Size: 953 1540 1747 977 1327
Fragments arranged by size:
1747 1540 1327 977 953
AgeI A'CCGG_T
Cuts at: 5375 5375
Size: 6544
AhdI GACnn_n'nnGTC
Cuts at: 5273 5273
Size: 6544
AleI CACnn'nnGTG
Cuts at: 1166 3413 1166
Size: 2247 4297
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3108 1184
Size: 1924 4620
AlwNI CAG_nnn'CTG
Cuts at: 2251 4287 5859 2251
Size: 2036 1572 2936
Fragments arranged by size:
2936 2036 1572
ApaI G_GGCC'C
Cuts at: 3529 3529
Size: 6544
ApaLI G'TGCA_C
Cuts at: 2070 4601 5757 2070
Size: 2531 1156 2857
Fragments arranged by size:
2857 2531 1156
ApoI r'AATT_y
Cuts at: 1493 3853 4079 4231 6340 1493
Size: 2360 226 152 2109 1697
Fragments arranged by size:
2360 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5098 160
Size: 872 4066 1606
Fragments arranged by size:
4066 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6544
AvaI C'yCGr_G
Cuts at: 1870 1943 2026 3869 1870
Size: 73 83 1843 4545
Fragments arranged by size:
4545 1843 83 73
AvrII C'CTAG_G
Cuts at: 2536 2536
Size: 6544
BamHI G'GATC_C
Cuts at: 3874 4241 3874
Size: 367 6177
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2013 2199 2320 2353 2409
Size: 325 217 627 226 186 121 33 56
Cuts at: 2409 2540 2981 3865 5320 618
Size: 131 441 884 1455 1842
Fragments arranged by size:
1842 1455 884 627 441 325 226 217
186 131 121 56 33
BanII G_rGCy'C
Cuts at: 1391 2516 2747 2895 3030 3056 3212 3309 3529
Size: 1125 231 148 135 26 156 97 220
Cuts at: 3529 3630 3687 3863 1391
Size: 101 57 176 4072
Fragments arranged by size:
4072 1125 231 220 176 156 148 135
101 97 57 26
BbeI G_GCGC'C
Cuts at: 2985 2985
Size: 6544
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2312 3816 928
Size: 971 413 1504 3656
Fragments arranged by size:
3656 1504 971 413
BbvCI CC'TCA_GC
Cuts at: 2205 2393 2205
Size: 188 6356
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4735 4769 1903
Size: 34 2798 34 3678
Fragments arranged by size:
3678 2798 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4436 5646 4436
Size: 1210 5334
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6544
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2997 3185 3533
Size: 107 122 71 631 605 1325 188 348
Cuts at: 3533 5155 136
Size: 1622 1525
Fragments arranged by size:
1622 1525 1325 631 605 348 188 122
107 71
BglII A'GATC_T
Cuts at: 3037 6539 3037
Size: 3502 3042
BlpI GC'TnA_GC
Cuts at: 2167 3545 3671 3961 2167
Size: 1378 126 290 4750
Fragments arranged by size:
4750 1378 290 126
Bme1580I G_kGCm'C
Cuts at: 1790 2074 2377 2545 3181 3529 4605 5761 1790
Size: 284 303 168 636 348 1076 1156 2573
Fragments arranged by size:
2573 1156 1076 636 348 303 284 168
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6544
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2339 4275 5233
Size: 416 53 83 399 24 922 1936 958
Cuts at: 5233 442
Size: 1753
Fragments arranged by size:
1936 1753 958 922 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6544
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2220 3049 3592 2220
Size: 829 543 5172
Fragments arranged by size:
5172 829 543
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2159 2282 2449 2603 2633 2805 2873 3100
Size: 920 123 167 154 30 172 68 227
Cuts at: 3100 3530 5204 1239
Size: 430 1674 2579
Fragments arranged by size:
2579 1674 920 430 227 172 167 154
123 68 30
Bpu10I CC'TnA_GC
Cuts at: 2205 2393 3419 2205
Size: 188 1026 5330
Fragments arranged by size:
5330 1026 188
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2579 2731 3188 4669 5534 5832 2579
Size: 152 457 1481 865 298 3291
Fragments arranged by size:
3291 1481 865 457 298 152
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3473 5207 882
Size: 617 1974 1734 2219
Fragments arranged by size:
2219 1974 1734 617
BsaAI yAC'GTr
Cuts at: 493 1204 3415 4335 493
Size: 711 2211 920 2702
Fragments arranged by size:
2702 2211 920 711
BsaBI GATnn'nnATC
Cuts at: 6538 6538
Size: 6544
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2982 4351 4733 5368
Size: 53 83 186 936 1449 1369 382 635
Cuts at: 5368 275
Size: 1451
Fragments arranged by size:
1451 1449 1369 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3486 4977 5375 5649 5796 6207 3486
Size: 1491 398 274 147 411 3823
Fragments arranged by size:
3823 1491 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2819 2849 1178
Size: 30 165 30 1416 30 4873
Fragments arranged by size:
4873 1416 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3168 3317 3527 1186
Size: 1982 149 210 4203
Fragments arranged by size:
4203 1982 210 149
BseYI C'CCAG_C
Cuts at: 2315 2387 2677 2740 3324 3385 5747 6243 2315
Size: 72 290 63 584 61 2362 496 2616
Fragments arranged by size:
2616 2362 584 496 290 72 63 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2086 2099 2357 2416 3602 3691 1163
Size: 573 350 13 258 59 1186 89 4016
Fragments arranged by size:
4016 1186 573 350 258 89 59 13
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2423 3001 3994 4755 5783 1540
Size: 275 186 422 578 993 761 1028 2301
Fragments arranged by size:
2301 1028 993 761 578 422 275 186
BsiHKAI G_wGCw'C
Cuts at: 2074 3309 3420 4605 4690 5761 2074
Size: 1235 111 1185 85 1071 2857
Fragments arranged by size:
2857 1235 1185 1071 111 85
BsmI GAATG_Cn'
Cuts at: 4055 4148 4055
Size: 93 6451
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2074 2377 2516 2545 2747 2895 3030
Size: 399 284 303 139 29 202 148 135
Cuts at: 3030 3056 3181 3212 3309 3420 3529 3630 3687
Size: 26 125 31 97 111 109 101 57
Cuts at: 3687 3863 4605 4690 5761 1391
Size: 176 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 303 284 202 176
148 139 135 125 111 109 101 97
85 57 31 29 26
BspEI T'CCGG_A
Cuts at: 6207 6207
Size: 6544
BspHI T'CATG_A
Cuts at: 4433 6163 4433
Size: 1730 4814
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2874 3216 3899 844
Size: 2030 342 683 3489
Fragments arranged by size:
3489 2030 683 342
BsrBI CCG'CTC
Cuts at: 1394 1823 2884 3636 4431 1394
Size: 429 1061 752 795 3507
Fragments arranged by size:
3507 1061 795 752 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2552 5039 5213 65
Size: 1183 1304 2487 174 1396
Fragments arranged by size:
2487 1396 1304 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2406 2501 3663 3710 5188 5375 1700
Size: 110 596 95 1162 47 1478 187 2869
Fragments arranged by size:
2869 1478 1162 596 187 110 95 47
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6544
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6544
BssSI C'ACGA_G
Cuts at: 4604 5616 4604
Size: 1012 5532
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6544
BstBI TT'CG_AA
Cuts at: 3363 5348 3363
Size: 1985 4559
BstEII G'GTnAC_C
Cuts at: 1184 2030 1184
Size: 846 5698
BstXI CCAn_nnnn'nTGG
Cuts at: 3022 3745 3022
Size: 723 5821
BstYI r'GATC_y
Cuts at: 1972 3037 3874 4241 4626 4643 6095 6181 6193
Size: 1065 837 367 385 17 1452 86 12
Cuts at: 6193 6539 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1065 837 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2977 513
Size: 552 939 973 4080
Fragments arranged by size:
4080 973 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2882 487
Size: 683 56 1656 4149
Fragments arranged by size:
4149 1656 683 56
BtsI GCAGTG_nn'
Cuts at: 2074 2986 3556 3603 4060 4854 4874 2074
Size: 912 570 47 457 794 20 3744
Fragments arranged by size:
3744 912 794 570 457 47 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6544
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6509
DraI TTT'AAA
Cuts at: 3848 3848
Size: 6544
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5551 809
Size: 507 4235 1802
Fragments arranged by size:
4235 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2037 2114
Size: 54 1425 50 275 18 177 30 77
Cuts at: 2114 2215 2420 2499 2998 3186 3991 4880 6509
Size: 101 205 79 499 188 805 889 1629
Cuts at: 6509 8
Size: 43
Fragments arranged by size:
1629 1425 889 805 499 275 205 188
177 101 79 77 54 50 43 30
18
EagI C'GGCC_G
Cuts at: 1537 1812 2420 2998 3991 1537
Size: 275 608 578 993 4090
Fragments arranged by size:
4090 993 608 578 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2936 4474 1353
Size: 1583 1538 3423
Fragments arranged by size:
3423 1583 1538
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5131 5505 5651 387
Size: 1064 138 3542 374 146 1280
Fragments arranged by size:
3542 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3307 3307
Size: 6544
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2159 2282 2449 2603 2633 2784 2805
Size: 393 527 123 167 154 30 151 21
Cuts at: 2805 2873 3100 3116 3530 4607 5204 5991 6259
Size: 68 227 16 414 1077 597 787 268
Cuts at: 6259 1239
Size: 1524
Fragments arranged by size:
1524 1077 787 597 527 414 393 268
227 167 154 151 123 68 30 21
16
EcoNI CCTnn'n_nnAGG
Cuts at: 3299 3299
Size: 6544
EcoO109I rG'GnC_Cy
Cuts at: 2248 2341 3370 3424 3525 2248
Size: 93 1029 54 101 5267
Fragments arranged by size:
5267 1029 101 93 54
EcoRI G'AATT_C
Cuts at: 3853 4231 3853
Size: 378 6166
FspI TGC'GCA
Cuts at: 5050 5050
Size: 6544
HaeII r_GCGC'y
Cuts at: 1678 2985 5691 1678
Size: 1307 2706 2531
Fragments arranged by size:
2706 2531 1307
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2819 2851 3186 3218 5185
Size: 32 135 32 1247 32 335 32 1967
Cuts at: 5185 5217 5259 5291 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1967 1247 335 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3888 4134 669
Size: 988 281 1950 246 3079
Fragments arranged by size:
3079 1950 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6544
HpaI GTT'AAC
Cuts at: 4134 4134
Size: 6544
KasI G'GCGC_C
Cuts at: 2981 2981
Size: 6544
KpnI G_GTAC'C
Cuts at: 3869 3869
Size: 6544
MfeI C'AATT_G
Cuts at: 4143 4143
Size: 6544
MluI A'CGCG_T
Cuts at: 6420 6420
Size: 6544
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2502 3312 5658 5842 1305
Size: 1197 810 2346 184 2007
Fragments arranged by size:
2346 2007 1197 810 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2217 3188 10
Size: 54 1425 343 177 208 971 3366
Fragments arranged by size:
3366 1425 971 343 208 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2155 3413 3713 4502 4861 5020 518
Size: 648 989 1258 300 789 359 159 2042
Fragments arranged by size:
2042 1258 989 789 648 359 300 159
MspA1I CmG'CkG
Cuts at: 1606 2083 2611 2851 3247 3385 3960 4637 5785
Size: 477 528 240 396 138 575 677 1148
Cuts at: 5785 6030 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 575 528 477 396 245
240 138
NaeI GCC'GGC
Cuts at: 2503 3665 2503
Size: 1162 5382
NarI GG'CG_CC
Cuts at: 2982 2982
Size: 6544
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5053
Fragments arranged by size:
5053 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6544
NgoMIV G'CCGG_C
Cuts at: 2501 3663 2501
Size: 1162 5382
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6544
NotI GC'GGCC_GC
Cuts at: 3991 3991
Size: 6544
NspI r_CATG'y
Cuts at: 1132 1229 2160 2381 3277 3700 3902 5447 1132
Size: 97 931 221 896 423 202 1545 2229
Fragments arranged by size:
2229 1545 931 896 423 221 202 97
PasI CC'CwG_GG
Cuts at: 1348 3433 1348
Size: 2085 4459
PciI A'CATG_T
Cuts at: 2156 3696 5443 2156
Size: 1540 1747 3257
Fragments arranged by size:
3257 1747 1540
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2224 2251 2363 2965 1108
Size: 1116 27 112 602 4687
Fragments arranged by size:
4687 1116 602 112 27
PfoI T'CCnGG_A
Cuts at: 2250 2250
Size: 6544
PmeI GTTT'AAAC
Cuts at: 3848 3848
Size: 6544
PmlI CAC'GTG
Cuts at: 3415 3415
Size: 6544
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3782 3814 4613 4645 6126 6158 3782
Size: 32 799 32 1481 32 4168
Fragments arranged by size:
4168 1481 799 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2248 2341 3370 3424 2248
Size: 93 1029 54 5368
Fragments arranged by size:
5368 1029 93 54
PshAI GACnn'nnGTC
Cuts at: 1613 3000 1613
Size: 1387 5157
PsiI TTA'TAA
Cuts at: 4114 4114
Size: 6544
PspOMI G'GGCC_C
Cuts at: 3525 3525
Size: 6544
PstI C_TGCA'G
Cuts at: 830 3218 3563 3896 830
Size: 2388 345 333 3478
Fragments arranged by size:
3478 2388 345 333
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6544
PvuII CAG'CTG
Cuts at: 2083 2611 2851 3385 2083
Size: 528 240 534 5242
Fragments arranged by size:
5242 534 528 240
SacI G_AGCT'C
Cuts at: 3309 3309
Size: 6544
SalI G'TCGA_C
Cuts at: 1936 3886 1936
Size: 1950 4594
SbfI CC_TGCA'GG
Cuts at: 3896 3896
Size: 6544
ScaI AGT'ACT
Cuts at: 4792 6504 4792
Size: 1712 4832
SfcI C'TryA_G
Cuts at: 826 1045 2606 3214 3559 3892 5027 5708 5899
Size: 219 1561 608 345 333 1135 681 191
Cuts at: 5899 826
Size: 1471
Fragments arranged by size:
1561 1471 1135 681 608 345 333 219
191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3533 3533
Size: 6544
SfoI GGC'GCC
Cuts at: 2983 2983
Size: 6544
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6544
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6544
SmaI CCC'GGG
Cuts at: 1872 3871 1872
Size: 1999 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2594 2746 3203 4648 5549 5811
Size: 197 926 651 152 457 1445 901 262
Cuts at: 5811 820
Size: 1553
Fragments arranged by size:
1553 1445 926 901 651 457 262 197
152
SnaBI TAC'GTA
Cuts at: 493 4335 493
Size: 3842 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6544
SphI G_CATG'C
Cuts at: 1132 3902 1132
Size: 2770 3774
SspI AAT'ATT
Cuts at: 5 52 4468 5
Size: 47 4416 2081
Fragments arranged by size:
4416 2081 47
StuI AGG'CCT
Cuts at: 1517 2631 2782 3536 1517
Size: 1114 151 754 4525
Fragments arranged by size:
4525 1114 754 151
StyI C'CwwG_G
Cuts at: 513 1065 2004 2536 2545 3339 3738 3983 513
Size: 552 939 532 9 794 399 245 3074
Fragments arranged by size:
3074 939 794 552 532 399 245 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2160 3425 3614 4571 4588 4741 4926
Size: 1255 580 1265 189 957 17 153 185
Cuts at: 4926 5371 325
Size: 445 1498
Fragments arranged by size:
1498 1265 1255 957 580 445 189 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4790 6502 96
Size: 274 80 33 51 1443 2813 1712 138
Fragments arranged by size:
2813 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2135 2167 6504 6536 2135
Size: 32 4337 32 2143
Fragments arranged by size:
4337 2143 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6544
XbaI T'CTAG_A
Cuts at: 3880 3880
Size: 6544
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2095 2095
Size: 6544
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6544
XmaI C'CCGG_G
Cuts at: 1870 3869 1870
Size: 1999 4545
XmnI GAAnn'nnTTC
Cuts at: 4673 4673
Size: 6544
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4352 276
Size: 53 83 186 936 2818 2468
Fragments arranged by size:
2818 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BfrBI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI
NsiI PacI PspXI PsrI RsrII SacII SanDI SapI
SexAI SrfI SwaI