Restriction map for
pFN21AB5992
(Circular) (Six-base) MAPSORT of: pFN21AB5992.seq Check: 9767 from: 1 to: 6532
pFN21AB5992 6532 bp
With 182 enzymes: SgfI *
December 8, 2014 13:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4342 278
Size: 53 83 186 936 2806 2468
Fragments arranged by size:
2806 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3875 1937
Size: 1938 4594
Acc65I G'GTAC_C
Cuts at: 3853 3853
Size: 6532
AclI AA'CG_TT
Cuts at: 1563 4659 5032 1563
Size: 3096 373 3063
Fragments arranged by size:
3096 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2238 2994 3018 3523 3804 4595 5979 6247
Size: 606 756 24 505 281 791 1384 268
Cuts at: 6247 1632
Size: 1917
Fragments arranged by size:
1917 1384 791 756 606 505 281 268
24
AfeI AGC'GCT
Cuts at: 3636 3636
Size: 6532
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6335
AflIII A'CryG_T
Cuts at: 1203 5431 6408 1203
Size: 4228 977 1327
Fragments arranged by size:
4228 1327 977
AgeI A'CCGG_T
Cuts at: 5363 5363
Size: 6532
AhdI GACnn_n'nnGTC
Cuts at: 2887 5261 2887
Size: 2374 4158
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6532
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2620 1184
Size: 1436 5096
AlwNI CAG_nnn'CTG
Cuts at: 2435 4275 5847 2435
Size: 1840 1572 3120
Fragments arranged by size:
3120 1840 1572
ApaLI G'TGCA_C
Cuts at: 4589 5745 4589
Size: 1156 5376
ApoI r'AATT_y
Cuts at: 1493 2044 2313 2486 2689 3841 4067 4219 6328
Size: 551 269 173 203 1152 226 152 2109
Cuts at: 6328 1493
Size: 1697
Fragments arranged by size:
2109 1697 1152 551 269 226 203 173
152
AseI AT'TA_AT
Cuts at: 160 1032 5086 160
Size: 872 4054 1606
Fragments arranged by size:
4054 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6532
AvaI C'yCGr_G
Cuts at: 1870 1943 3857 1870
Size: 73 1914 4545
Fragments arranged by size:
4545 1914 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3442 3475 3442
Size: 33 6499
BamHI G'GATC_C
Cuts at: 3862 4229 3862
Size: 367 6165
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3853 5308 618
Size: 325 217 627 2066 1455 1842
Fragments arranged by size:
2066 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3851 1391
Size: 2460 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2244 2371 2469 3091 928
Size: 971 345 127 98 622 4369
Fragments arranged by size:
4369 971 622 345 127 98
BbvCI CC'TCA_GC
Cuts at: 3093 3093
Size: 6532
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4723 4757 1903
Size: 34 2786 34 3678
Fragments arranged by size:
3678 2786 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3125 4424 5634 3125
Size: 1299 1210 4023
Fragments arranged by size:
4023 1299 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6532
BfrBI ATG'CAT
Cuts at: 2213 2672 2213
Size: 459 6073
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5143 136
Size: 107 122 71 631 605 3471 1525
Fragments arranged by size:
3471 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6527 6527
Size: 6532
BlpI GC'TnA_GC
Cuts at: 3949 3949
Size: 6532
Bme1580I G_kGCm'C
Cuts at: 1790 4593 5749 1790
Size: 2803 1156 2573
Fragments arranged by size:
2803 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6532
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2155 4263 5221
Size: 416 53 83 399 24 738 2108 958
Cuts at: 5221 442
Size: 1753
Fragments arranged by size:
2108 1753 958 738 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6532
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2259 2385 3462 5192 1239
Size: 1020 126 1077 1730 2579
Fragments arranged by size:
2579 1730 1077 1020 126
Bpu10I CC'TnA_GC
Cuts at: 3093 3624 3093
Size: 531 6001
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3728 4657 5522 5820 3728
Size: 929 865 298 4440
Fragments arranged by size:
4440 929 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5195 882
Size: 617 3696 2219
Fragments arranged by size:
3696 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4323 493
Size: 711 3119 2702
Fragments arranged by size:
3119 2702 711
BsaBI GATnn'nnATC
Cuts at: 3350 6526 3350
Size: 3176 3356
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4339 4721 5356 275
Size: 53 83 186 936 2806 382 635 1451
Fragments arranged by size:
2806 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4965 5363 5637 5784 6195 4965
Size: 398 274 147 411 5302
Fragments arranged by size:
5302 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6307
Fragments arranged by size:
6307 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2832 1186
Size: 1646 4886
BseYI C'CCAG_C
Cuts at: 3324 5735 6231 3324
Size: 2411 496 3625
Fragments arranged by size:
3625 2411 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2697 3490 1163
Size: 573 961 793 4205
Fragments arranged by size:
4205 961 793 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3982 4743 5771 1540
Size: 275 186 1981 761 1028 2301
Fragments arranged by size:
2301 1981 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2808 4593 4678 5749 2808
Size: 1785 85 1071 3591
Fragments arranged by size:
3591 1785 1071 85
BsmI GAATG_Cn'
Cuts at: 2609 2912 3185 3578 4043 4136 2609
Size: 303 273 393 465 93 5005
Fragments arranged by size:
5005 465 393 303 273 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2808 3851 4593 4678 5749 1391
Size: 399 1018 1043 742 85 1071 2174
Fragments arranged by size:
2174 1071 1043 1018 742 399 85
BspEI T'CCGG_A
Cuts at: 6195 6195
Size: 6532
BspHI T'CATG_A
Cuts at: 2478 3070 4421 6151 2478
Size: 592 1351 1730 2859
Fragments arranged by size:
2859 1730 1351 592
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2287 3887 844
Size: 1443 1600 3489
Fragments arranged by size:
3489 1600 1443
BsrBI CCG'CTC
Cuts at: 1394 1823 3431 4419 1394
Size: 429 1608 988 3507
Fragments arranged by size:
3507 1608 988 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3717 5027 5201 65
Size: 1183 2469 1310 174 1396
Fragments arranged by size:
2469 1396 1310 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5176 5363 1700
Size: 110 3366 187 2869
Fragments arranged by size:
3366 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2797 96
Size: 2701 3831
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6532
BssSI C'ACGA_G
Cuts at: 3813 4592 5604 3813
Size: 779 1012 4741
Fragments arranged by size:
4741 1012 779
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2219 794
Size: 1425 5107
BstBI TT'CG_AA
Cuts at: 5336 5336
Size: 6532
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6532
BstXI CCAn_nnnn'nTGG
Cuts at: 3364 3364
Size: 6532
BstYI r'GATC_y
Cuts at: 1972 3610 3862 4229 4614 4631 6083 6169 6181
Size: 1638 252 367 385 17 1452 86 12
Cuts at: 6181 6527 1972
Size: 346 1977
Fragments arranged by size:
1977 1638 1452 385 367 346 252 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5980
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5793
Fragments arranged by size:
5793 683 56
BtsI GCAGTG_nn'
Cuts at: 2063 4048 4842 4862 2063
Size: 1985 794 20 3733
Fragments arranged by size:
3733 1985 794 20
ClaI AT'CG_AT
Cuts at: 1554 2408 1554
Size: 854 5678
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6497
DraI TTT'AAA
Cuts at: 3836 3836
Size: 6532
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5539 809
Size: 507 4223 1802
Fragments arranged by size:
4223 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3348 3496 3979
Size: 54 1425 50 275 18 1518 148 483
Cuts at: 3979 4868 6497 8
Size: 889 1629 43
Fragments arranged by size:
1629 1518 1425 889 483 275 148 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3979 1537
Size: 275 2167 4090
Fragments arranged by size:
4090 2167 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2352 3263 3780 4462 1353
Size: 999 911 517 682 3423
Fragments arranged by size:
3423 999 911 682 517
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5119 5493 5639 387
Size: 1064 138 3530 374 146 1280
Fragments arranged by size:
3530 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2238 2259 2385 2994 3018 3462 3523
Size: 393 606 21 126 609 24 444 61
Cuts at: 3523 3804 4595 5192 5979 6247 1239
Size: 281 791 597 787 268 1524
Fragments arranged by size:
1524 791 787 609 606 597 444 393
281 268 126 61 24 21
EcoO109I rG'GnC_Cy
Cuts at: 2697 2697
Size: 6532
EcoRI G'AATT_C
Cuts at: 3841 4219 3841
Size: 378 6154
FspI TGC'GCA
Cuts at: 5038 5038
Size: 6532
HaeII r_GCGC'y
Cuts at: 1678 3638 5679 1678
Size: 1960 2041 2531
Fragments arranged by size:
2531 2041 1960
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2234 2266 2873 2905 5173
Size: 32 135 32 662 32 607 32 2268
Cuts at: 5173 5205 5247 5279 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2268 662 607 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3876 4122 669
Size: 988 281 1938 246 3079
Fragments arranged by size:
3079 1938 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6532
HpaI GTT'AAC
Cuts at: 4122 4122
Size: 6532
KpnI G_GTAC'C
Cuts at: 3857 3857
Size: 6532
MfeI C'AATT_G
Cuts at: 2223 4131 2223
Size: 1908 4624
MluI A'CGCG_T
Cuts at: 6408 6408
Size: 6532
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3318 3720 5646 5830 1305
Size: 2013 402 1926 184 2007
Fragments arranged by size:
2013 2007 1926 402 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3350 3498 10
Size: 54 1425 343 1518 148 3044
Fragments arranged by size:
3044 1518 1425 343 148 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2073 2673 2799 4490 4849 5008 518
Size: 648 907 600 126 1691 359 159 2042
Fragments arranged by size:
2042 1691 907 648 600 359 159 126
MspA1I CmG'CkG
Cuts at: 1606 3328 3948 4625 5773 6018 1606
Size: 1722 620 677 1148 245 2120
Fragments arranged by size:
2120 1722 1148 677 620 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5980
NdeI CA'TA_TG
Cuts at: 387 2455 2867 3517 3565 387
Size: 2068 412 650 48 3354
Fragments arranged by size:
3354 2068 650 412 48
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6532
NotI GC'GGCC_GC
Cuts at: 3979 3979
Size: 6532
NsiI A_TGCA'T
Cuts at: 2215 2674 2215
Size: 459 6073
NspI r_CATG'y
Cuts at: 1132 1229 2672 2676 3890 5435 1132
Size: 97 1443 4 1214 1545 2229
Fragments arranged by size:
2229 1545 1443 1214 97 4
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6532
PciI A'CATG_T
Cuts at: 5431 5431
Size: 6532
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 3235 1108
Size: 902 1225 4405
Fragments arranged by size:
4405 1225 902
PfoI T'CCnGG_A
Cuts at: 2781 3219 2781
Size: 438 6094
PmeI GTTT'AAAC
Cuts at: 3836 3836
Size: 6532
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4601 4633 6114 6146 4601
Size: 32 1481 32 4987
Fragments arranged by size:
4987 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2697 2697
Size: 6532
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6532
PsiI TTA'TAA
Cuts at: 4102 4102
Size: 6532
PstI C_TGCA'G
Cuts at: 830 2122 2348 2841 3884 830
Size: 1292 226 493 1043 3478
Fragments arranged by size:
3478 1292 1043 493 226
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6532
PvuII CAG'CTG
Cuts at: 3328 3328
Size: 6532
SalI G'TCGA_C
Cuts at: 1936 3874 1936
Size: 1938 4594
SanDI GG'GwC_CC
Cuts at: 2697 2697
Size: 6532
SbfI CC_TGCA'GG
Cuts at: 3884 3884
Size: 6532
ScaI AGT'ACT
Cuts at: 4780 6492 4780
Size: 1712 4820
SfcI C'TryA_G
Cuts at: 826 1045 2118 2344 2837 3880 5015 5696 5887
Size: 219 1073 226 493 1043 1135 681 191
Cuts at: 5887 826
Size: 1471
Fragments arranged by size:
1471 1135 1073 1043 681 493 226 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6532
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6532
SmaI CCC'GGG
Cuts at: 1872 3859 1872
Size: 1987 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3707 4636 5537 5799 820
Size: 197 926 1764 929 901 262 1553
Fragments arranged by size:
1764 1553 929 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4323 493
Size: 3830 2702
SpeI A'CTAG_T
Cuts at: 152 2776 152
Size: 2624 3908
SphI G_CATG'C
Cuts at: 1132 3890 1132
Size: 2758 3774
SspI AAT'ATT
Cuts at: 5 52 3077 3278 4456 5
Size: 47 3025 201 1178 2081
Fragments arranged by size:
3025 2081 1178 201 47
StuI AGG'CCT
Cuts at: 1517 3735 1517
Size: 2218 4314
StyI C'CwwG_G
Cuts at: 513 1065 3754 3971 513
Size: 552 2689 217 3074
Fragments arranged by size:
3074 2689 552 217
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2002 4559 4576 4729 4914 5359 325
Size: 1255 422 2557 17 153 185 445 1498
Fragments arranged by size:
2557 1498 1255 445 422 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2114 2797 4778
Size: 274 80 33 51 1443 137 683 1981
Cuts at: 4778 6490 96
Size: 1712 138
Fragments arranged by size:
1981 1712 1443 683 274 138 137 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6492 6524 6492
Size: 32 6500
Tth111I GACn'n_nGTC
Cuts at: 1316 3334 1316
Size: 2018 4514
XbaI T'CTAG_A
Cuts at: 3868 3868
Size: 6532
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 6532
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6532
XmaI C'CCGG_G
Cuts at: 1870 3857 1870
Size: 1987 4545
XmnI GAAnn'nnTTC
Cuts at: 2089 2486 3155 4661 2089
Size: 397 669 1506 3960
Fragments arranged by size:
3960 1506 669 397
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4340 276
Size: 53 83 186 936 2806 2468
Fragments arranged by size:
2806 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbeI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI
SexAI SfiI SfoI SrfI SwaI