Restriction map for
pFN21AB5989
(Circular) (Six-base) MAPSORT of: pFN21AB5989.seq Check: 6015 from: 1 to: 6424
pFN21AB5989 6424 bp
With 182 enzymes: SgfI *
July 6, 2012 17:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2336 2336
Size: 6424
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4234 278
Size: 53 83 186 936 2698 2468
Fragments arranged by size:
2698 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3767 1937
Size: 1830 4594
Acc65I G'GTAC_C
Cuts at: 3745 3745
Size: 6424
AclI AA'CG_TT
Cuts at: 1563 4551 4924 1563
Size: 2988 373 3063
Fragments arranged by size:
3063 2988 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3646 4487 5871 6139 1632
Size: 2014 841 1384 268 1917
Fragments arranged by size:
2014 1917 1384 841 268
AfeI AGC'GCT
Cuts at: 2960 2960
Size: 6424
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6227
AflIII A'CryG_T
Cuts at: 1203 5323 6300 1203
Size: 4120 977 1327
Fragments arranged by size:
4120 1327 977
AgeI A'CCGG_T
Cuts at: 5255 5255
Size: 6424
AhdI GACnn_n'nnGTC
Cuts at: 5153 5153
Size: 6424
AleI CACnn'nnGTG
Cuts at: 1166 2045 1166
Size: 879 5545
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6424
AlwNI CAG_nnn'CTG
Cuts at: 2181 2580 2602 2895 2938 3434 3517 4167 5739
Size: 399 22 293 43 496 83 650 1572
Cuts at: 5739 2181
Size: 2866
Fragments arranged by size:
2866 1572 650 496 399 293 83 43
22
ApaI G_GGCC'C
Cuts at: 2829 2829
Size: 6424
ApaLI G'TGCA_C
Cuts at: 4481 5637 4481
Size: 1156 5268
ApoI r'AATT_y
Cuts at: 1493 3733 3959 4111 6220 1493
Size: 2240 226 152 2109 1697
Fragments arranged by size:
2240 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4978 160
Size: 872 3946 1606
Fragments arranged by size:
3946 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6424
AvaI C'yCGr_G
Cuts at: 1870 1943 2292 3749 1870
Size: 73 349 1457 4545
Fragments arranged by size:
4545 1457 349 73
BamHI G'GATC_C
Cuts at: 3754 4121 3754
Size: 367 6057
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3149 3166 3745 5200 618
Size: 325 217 627 1362 17 579 1455 1842
Fragments arranged by size:
1842 1455 1362 627 579 325 217 17
BanII G_rGCy'C
Cuts at: 1391 2151 2299 2390 2829 2861 3177 3453 3743
Size: 760 148 91 439 32 316 276 290
Cuts at: 3743 1391
Size: 4072
Fragments arranged by size:
4072 760 439 316 290 276 148 91
32
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3465 3603 928
Size: 971 1566 138 3749
Fragments arranged by size:
3749 1566 971 138
BbvCI CC'TCA_GC
Cuts at: 3443 3642 3696 3443
Size: 199 54 6171
Fragments arranged by size:
6171 199 54
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4615 4649 1903
Size: 34 50 34 2594 34 3678
Fragments arranged by size:
3678 2594 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4316 5526 4316
Size: 1210 5214
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6424
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2693 3326 5035
Size: 107 122 71 631 605 1021 633 1709
Cuts at: 5035 136
Size: 1525
Fragments arranged by size:
1709 1525 1021 633 631 605 122 107
71
BglII A'GATC_T
Cuts at: 3001 6419 3001
Size: 3418 3006
BlpI GC'TnA_GC
Cuts at: 3841 3841
Size: 6424
Bme1580I G_kGCm'C
Cuts at: 1790 2221 2829 4485 5641 1790
Size: 431 608 1656 1156 2573
Fragments arranged by size:
2573 1656 1156 608 431
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6424
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2129 2875 2905
Size: 416 53 83 399 24 712 746 30
Cuts at: 2905 3344 4155 5113 442
Size: 439 811 958 1753
Fragments arranged by size:
1753 958 811 746 712 439 416 399
83 53 30 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6424
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2115 2364 2602 5084 1239
Size: 876 249 238 2482 2579
Fragments arranged by size:
2579 2482 876 249 238
Bpu10I CC'TnA_GC
Cuts at: 2607 3443 3642 3696 2607
Size: 836 199 54 5335
Fragments arranged by size:
5335 836 199 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3522 4549 5414 5712 3522
Size: 1027 865 298 4234
Fragments arranged by size:
4234 1027 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3048 5087 882
Size: 617 1549 2039 2219
Fragments arranged by size:
2219 2039 1549 617
BsaAI yAC'GTr
Cuts at: 493 1204 3572 4215 493
Size: 711 2368 643 2702
Fragments arranged by size:
2702 2368 711 643
BsaBI GATnn'nnATC
Cuts at: 6418 6418
Size: 6424
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3391 4231 4613 5248
Size: 53 83 186 936 1858 840 382 635
Cuts at: 5248 275
Size: 1451
Fragments arranged by size:
1858 1451 936 840 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4857 5255 5529 5676 6087 4857
Size: 398 274 147 411 5194
Fragments arranged by size:
5194 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3700 3730 1178
Size: 30 165 30 2297 30 3872
Fragments arranged by size:
3872 2297 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2367 2462 2465 2969 3400 1186
Size: 1181 95 3 504 431 4210
Fragments arranged by size:
4210 1181 504 431 95 3
BseYI C'CCAG_C
Cuts at: 2303 2458 2488 3127 5627 6123 2303
Size: 155 30 639 2500 496 2604
Fragments arranged by size:
2604 2500 639 496 155 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3398 1163
Size: 573 1662 4189
Fragments arranged by size:
4189 1662 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3874 4635 5663 1540
Size: 275 186 1873 761 1028 2301
Fragments arranged by size:
2301 1873 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2151 3024 3078 3177 3453 4485 4570 5641 2151
Size: 873 54 99 276 1032 85 1071 2934
Fragments arranged by size:
2934 1071 1032 873 276 99 85 54
BsmI GAATG_Cn'
Cuts at: 3935 4028 3935
Size: 93 6331
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2151 2221 2299 2390 2829 2861 3024
Size: 399 361 70 78 91 439 32 163
Cuts at: 3024 3078 3177 3453 3743 4485 4570 5641 1391
Size: 54 99 276 290 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 439 399 361 290 276
163 99 91 85 78 70 54 32
BspEI T'CCGG_A
Cuts at: 6087 6087
Size: 6424
BspHI T'CATG_A
Cuts at: 4313 6043 4313
Size: 1730 4694
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2336 3779 844
Size: 1492 1443 3489
Fragments arranged by size:
3489 1492 1443
BsrBI CCG'CTC
Cuts at: 1394 1823 4311 1394
Size: 429 2488 3507
Fragments arranged by size:
3507 2488 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2359 4919 5093 65
Size: 1183 1111 2560 174 1396
Fragments arranged by size:
2560 1396 1183 1111 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5068 5255 1700
Size: 110 3258 187 2869
Fragments arranged by size:
3258 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3505 96
Size: 3409 3015
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6424
BssSI C'ACGA_G
Cuts at: 4484 5496 4484
Size: 1012 5412
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2602 794
Size: 1808 4616
BstBI TT'CG_AA
Cuts at: 5228 5228
Size: 6424
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6424
BstXI CCAn_nnnn'nTGG
Cuts at: 2047 2443 3411 2047
Size: 396 968 5060
Fragments arranged by size:
5060 968 396
BstYI r'GATC_y
Cuts at: 1972 2193 3001 3754 4121 4506 4523 5975 6061
Size: 221 808 753 367 385 17 1452 86
Cuts at: 6061 6073 6419 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 808 753 385 367 346 221
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5872
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5685
Fragments arranged by size:
5685 683 56
BtsI GCAGTG_nn'
Cuts at: 2655 2787 3381 3940 4734 4754 2655
Size: 132 594 559 794 20 4325
Fragments arranged by size:
4325 794 594 559 132 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6424
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6389
DraI TTT'AAA
Cuts at: 3728 3728
Size: 6424
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2774 5431 809
Size: 507 1458 2657 1802
Fragments arranged by size:
2657 1802 1458 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2809 2971 3871
Size: 54 1425 50 275 18 979 162 900
Cuts at: 3871 4760 6389 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 979 900 889 275 162 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3871 1537
Size: 275 2059 4090
Fragments arranged by size:
4090 2059 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2327 4354 1353
Size: 974 2027 3423
Fragments arranged by size:
3423 2027 974
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5011 5385 5531 387
Size: 1064 138 3422 374 146 1280
Fragments arranged by size:
3422 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2149 3175 3451 2149
Size: 1026 276 5122
Fragments arranged by size:
5122 1026 276
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2115 2364 2602 3646 4487 5084 5871
Size: 393 483 249 238 1044 841 597 787
Cuts at: 5871 6139 1239
Size: 268 1524
Fragments arranged by size:
1524 1044 841 787 597 483 393 268
249 238
EcoNI CCTnn'n_nnAGG
Cuts at: 2379 2930 2379
Size: 551 5873
EcoO109I rG'GnC_Cy
Cuts at: 2089 2411 2804 2826 2935 3677 2089
Size: 322 393 22 109 742 4836
Fragments arranged by size:
4836 742 393 322 109 22
EcoRI G'AATT_C
Cuts at: 3733 4111 3733
Size: 378 6046
FspI TGC'GCA
Cuts at: 4930 4930
Size: 6424
HaeII r_GCGC'y
Cuts at: 1678 2962 3418 3517 5571 1678
Size: 1284 456 99 2054 2531
Fragments arranged by size:
2531 2054 1284 456 99
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3097 3129 3221 3253 3376
Size: 32 135 32 1525 32 92 32 123
Cuts at: 3376 3408 5065 5097 5139 5171 1373
Size: 32 1657 32 42 32 2626
Fragments arranged by size:
2626 1657 1525 135 123 92 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3768 4014 669
Size: 988 281 1830 246 3079
Fragments arranged by size:
3079 1830 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6424
HpaI GTT'AAC
Cuts at: 4014 4014
Size: 6424
KpnI G_GTAC'C
Cuts at: 3749 3749
Size: 6424
MfeI C'AATT_G
Cuts at: 4023 4023
Size: 6424
MluI A'CGCG_T
Cuts at: 6300 6300
Size: 6424
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3244 3337 5538 5722 1305
Size: 1939 93 2201 184 2007
Fragments arranged by size:
2201 2007 1939 184 93
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2973 10
Size: 54 1425 343 1141 3461
Fragments arranged by size:
3461 1425 1141 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2045 2441 4382 4741 4900 518
Size: 648 879 396 1941 359 159 2042
Fragments arranged by size:
2042 1941 879 648 396 359 159
MspA1I CmG'CkG
Cuts at: 1606 2045 2166 2181 2668 3131 3273 3497 3646
Size: 439 121 15 487 463 142 224 149
Cuts at: 3646 3840 4517 5665 5910 1606
Size: 194 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 487 463 439 245 224
194 149 142 121 15
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5872
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6424
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6424
NotI GC'GGCC_GC
Cuts at: 3871 3871
Size: 6424
NspI r_CATG'y
Cuts at: 1132 1229 3782 5327 1132
Size: 97 2553 1545 2229
Fragments arranged by size:
2553 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2390 2407 2932 1348
Size: 1042 17 525 4840
Fragments arranged by size:
4840 1042 525 17
PciI A'CATG_T
Cuts at: 5323 5323
Size: 6424
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2895 2938 1108
Size: 1787 43 4594
Fragments arranged by size:
4594 1787 43
PfoI T'CCnGG_A
Cuts at: 2631 3686 2631
Size: 1055 5369
PmeI GTTT'AAAC
Cuts at: 3728 3728
Size: 6424
PmlI CAC'GTG
Cuts at: 3572 3572
Size: 6424
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4493 4525 6006 6038 4493
Size: 32 1481 32 4879
Fragments arranged by size:
4879 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2411 2804 2411
Size: 393 6031
PshAI GACnn'nnGTC
Cuts at: 1613 2286 3297 1613
Size: 673 1011 4740
Fragments arranged by size:
4740 1011 673
PsiI TTA'TAA
Cuts at: 3994 3994
Size: 6424
PspOMI G'GGCC_C
Cuts at: 2825 2825
Size: 6424
PstI C_TGCA'G
Cuts at: 830 2598 3776 830
Size: 1768 1178 3478
Fragments arranged by size:
3478 1768 1178
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6424
PvuII CAG'CTG
Cuts at: 2045 2668 3131 3497 2045
Size: 623 463 366 4972
Fragments arranged by size:
4972 623 463 366
SacI G_AGCT'C
Cuts at: 2151 3177 3453 2151
Size: 1026 276 5122
Fragments arranged by size:
5122 1026 276
SalI G'TCGA_C
Cuts at: 1936 3766 1936
Size: 1830 4594
SanDI GG'GwC_CC
Cuts at: 2411 2411
Size: 6424
SbfI CC_TGCA'GG
Cuts at: 3776 3776
Size: 6424
ScaI AGT'ACT
Cuts at: 4672 6384 4672
Size: 1712 4712
SexAI A'CCwGG_T
Cuts at: 2983 2983
Size: 6424
SfcI C'TryA_G
Cuts at: 826 1045 2173 2513 2527 2594 2759 3197 3634
Size: 219 1128 340 14 67 165 438 437
Cuts at: 3634 3772 4907 5588 5779 826
Size: 138 1135 681 191 1471
Fragments arranged by size:
1471 1135 1128 681 438 437 340 219
191 165 138 67 14
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6424
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6424
SmaI CCC'GGG
Cuts at: 1872 3751 1872
Size: 1879 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3537 4528 5429 5691 820
Size: 197 926 1594 991 901 262 1553
Fragments arranged by size:
1594 1553 991 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4215 493
Size: 3722 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6424
SphI G_CATG'C
Cuts at: 1132 3782 1132
Size: 2650 3774
SspI AAT'ATT
Cuts at: 5 52 4348 5
Size: 47 4296 2081
Fragments arranged by size:
4296 2081 47
StuI AGG'CCT
Cuts at: 1517 2696 2757 3289 3640 1517
Size: 1179 61 532 351 4301
Fragments arranged by size:
4301 1179 532 351 61
StyI C'CwwG_G
Cuts at: 513 1065 2558 2853 3069 3369 3525 3863 513
Size: 552 1493 295 216 300 156 338 3074
Fragments arranged by size:
3074 1493 552 338 300 295 216 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3167 4451 4468 4621 4806 5251 325
Size: 1255 1587 1284 17 153 185 445 1498
Fragments arranged by size:
1587 1498 1284 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2883 3505 3602
Size: 274 80 33 51 1443 906 622 97
Cuts at: 3602 4670 6382 96
Size: 1068 1712 138
Fragments arranged by size:
1712 1443 1068 906 622 274 138 97
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3294 3326 6384 6416 3294
Size: 32 3058 32 3302
Fragments arranged by size:
3302 3058 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6424
XbaI T'CTAG_A
Cuts at: 3760 3760
Size: 6424
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2306 3055 2306
Size: 749 5675
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6424
XmaI C'CCGG_G
Cuts at: 1870 3749 1870
Size: 1879 4545
XmnI GAAnn'nnTTC
Cuts at: 4553 4553
Size: 6424
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4232 276
Size: 53 83 186 936 2698 2468
Fragments arranged by size:
2698 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI
PsrI RsrII SacII SapI SfiI SfoI SrfI SwaI