Restriction map for
pFN21AB5986
(Circular) (Six-base) MAPSORT of: pFN21AB5986.seq Check: 7262 from: 1 to: 6412
pFN21AB5986 6412 bp
With 182 enzymes: SgfI *
July 6, 2012 17:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4222 278
Size: 53 83 186 936 2686 2468
Fragments arranged by size:
2686 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2718 3200 3755 1937
Size: 781 482 555 4594
Fragments arranged by size:
4594 781 555 482
Acc65I G'GTAC_C
Cuts at: 3609 3733 3609
Size: 124 6288
AclI AA'CG_TT
Cuts at: 1563 4539 4912 1563
Size: 2976 373 3063
Fragments arranged by size:
3063 2976 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3126 3370 3429 4475 5859 6127 1632
Size: 1494 244 59 1046 1384 268 1917
Fragments arranged by size:
1917 1494 1384 1046 268 244 59
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6215
AflIII A'CryG_T
Cuts at: 1203 5311 6288 1203
Size: 4108 977 1327
Fragments arranged by size:
4108 1327 977
AgeI A'CCGG_T
Cuts at: 5243 5243
Size: 6412
AhdI GACnn_n'nnGTC
Cuts at: 5141 5141
Size: 6412
AleI CACnn'nnGTG
Cuts at: 1166 2526 1166
Size: 1360 5052
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6412
AlwNI CAG_nnn'CTG
Cuts at: 3658 4155 5727 3658
Size: 497 1572 4343
Fragments arranged by size:
4343 1572 497
ApaLI G'TGCA_C
Cuts at: 4469 5625 4469
Size: 1156 5256
ApoI r'AATT_y
Cuts at: 1493 2302 2339 2645 3515 3721 3947 4099 6208
Size: 809 37 306 870 206 226 152 2109
Cuts at: 6208 1493
Size: 1697
Fragments arranged by size:
2109 1697 870 809 306 226 206 152
37
AseI AT'TA_AT
Cuts at: 160 1032 3324 4966 160
Size: 872 2292 1642 1606
Fragments arranged by size:
2292 1642 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6412
AvaI C'yCGr_G
Cuts at: 1870 1943 2634 3707 3737 1870
Size: 73 691 1073 30 4545
Fragments arranged by size:
4545 1073 691 73 30
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2184 2217 2184
Size: 33 6379
BamHI G'GATC_C
Cuts at: 2726 3742 4109 2726
Size: 1016 367 5029
Fragments arranged by size:
5029 1016 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3609 3733 5188 618
Size: 325 217 627 1822 124 1455 1842
Fragments arranged by size:
1842 1822 1455 627 325 217 124
BanII G_rGCy'C
Cuts at: 1391 2696 3731 1391
Size: 1305 1035 4072
Fragments arranged by size:
4072 1305 1035
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2838 3312 3355 928
Size: 971 939 474 43 3985
Fragments arranged by size:
3985 971 939 474 43
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4603 4637 1903
Size: 34 2666 34 3678
Fragments arranged by size:
3678 2666 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3186 4304 5514 3186
Size: 1118 1210 4084
Fragments arranged by size:
4084 1210 1118
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6412
BfrBI ATG'CAT
Cuts at: 2597 2597
Size: 6412
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5023 136
Size: 107 122 71 631 605 3351 1525
Fragments arranged by size:
3351 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6407 6407
Size: 6412
BlpI GC'TnA_GC
Cuts at: 3829 3829
Size: 6412
Bme1580I G_kGCm'C
Cuts at: 1790 2432 4473 5629 1790
Size: 642 2041 1156 2573
Fragments arranged by size:
2573 2041 1156 642
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6412
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2813 4143 5101
Size: 416 53 83 399 24 1396 1330 958
Cuts at: 5101 442
Size: 1753
Fragments arranged by size:
1753 1396 1330 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6412
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2078 2203 3494 5072 1239
Size: 839 125 1291 1578 2579
Fragments arranged by size:
2579 1578 1291 839 125
Bpu10I CC'TnA_GC
Cuts at: 2262 2262
Size: 6412
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3488 4537 5402 5700 3488
Size: 1049 865 298 4200
Fragments arranged by size:
4200 1049 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5075 882
Size: 617 3576 2219
Fragments arranged by size:
3576 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3700 4203 493
Size: 711 2496 503 2702
Fragments arranged by size:
2702 2496 711 503
BsaBI GATnn'nnATC
Cuts at: 6406 6406
Size: 6412
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4219 4601 5236 275
Size: 53 83 186 936 2686 382 635 1451
Fragments arranged by size:
2686 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4845 5243 5517 5664 6075 4845
Size: 398 274 147 411 5182
Fragments arranged by size:
5182 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2409 2439 1178
Size: 30 165 30 1006 30 5151
Fragments arranged by size:
5151 1006 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6412
BseYI C'CCAG_C
Cuts at: 2695 3394 5615 6111 2695
Size: 699 2221 496 2996
Fragments arranged by size:
2996 2221 699 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5839
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3862 4623 5651 1540
Size: 275 186 1861 761 1028 2301
Fragments arranged by size:
2301 1861 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4473 4558 5629 4473
Size: 85 1071 5256
Fragments arranged by size:
5256 1071 85
BsmI GAATG_Cn'
Cuts at: 3923 4016 3923
Size: 93 6319
BsmBI CGTCTCn'nnnn_
Cuts at: 2406 2406
Size: 6412
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2432 2696 3731 4473 4558 5629 1391
Size: 399 642 264 1035 742 85 1071 2174
Fragments arranged by size:
2174 1071 1035 742 642 399 264 85
BspEI T'CCGG_A
Cuts at: 6075 6075
Size: 6412
BspHI T'CATG_A
Cuts at: 4301 6031 4301
Size: 1730 4682
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3767 844
Size: 2923 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4299 1394
Size: 429 2476 3507
Fragments arranged by size:
3507 2476 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2772 4907 5081 65
Size: 1183 1524 2135 174 1396
Fragments arranged by size:
2135 1524 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2756 5056 5243 1700
Size: 110 946 2300 187 2869
Fragments arranged by size:
2869 2300 946 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6412
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6412
BssSI C'ACGA_G
Cuts at: 4472 5484 4472
Size: 1012 5400
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6412
BstBI TT'CG_AA
Cuts at: 5216 5216
Size: 6412
BstEII G'GTnAC_C
Cuts at: 1184 3633 1184
Size: 2449 3963
BstYI r'GATC_y
Cuts at: 1972 2726 3742 4109 4494 4511 5963 6049 6061
Size: 754 1016 367 385 17 1452 86 12
Cuts at: 6061 6407 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1016 754 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3240 3240
Size: 6412
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5860
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5673
Fragments arranged by size:
5673 683 56
BtsI GCAGTG_nn'
Cuts at: 3928 4722 4742 3928
Size: 794 20 5598
Fragments arranged by size:
5598 794 20
ClaI AT'CG_AT
Cuts at: 1554 3509 1554
Size: 1955 4457
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2325 2360 529
Size: 35 1761 35 4581
Fragments arranged by size:
4581 1761 35 35
DraI TTT'AAA
Cuts at: 2449 3229 3716 2449
Size: 780 487 5145
Fragments arranged by size:
5145 780 487
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5419 809
Size: 507 4103 1802
Fragments arranged by size:
4103 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2758 3859 4748
Size: 54 1425 50 275 18 928 1101 889
Cuts at: 4748 6377 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1101 928 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3859 1537
Size: 275 2047 4090
Fragments arranged by size:
4090 2047 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3405 4342 1353
Size: 2052 937 3423
Fragments arranged by size:
3423 2052 937
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4999 5373 5519 387
Size: 1064 138 3410 374 146 1280
Fragments arranged by size:
3410 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2078 2203 3126 3370 3429 3494 4475
Size: 393 446 125 923 244 59 65 981
Cuts at: 4475 5072 5859 6127 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 981 923 787 597 446 393 268
244 125 65 59
EcoRI G'AATT_C
Cuts at: 3721 4099 3721
Size: 378 6034
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2261 2261
Size: 6412
FspI TGC'GCA
Cuts at: 4918 4918
Size: 6412
HaeII r_GCGC'y
Cuts at: 1678 5559 1678
Size: 3881 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2038 2070 2711 2743 3342
Size: 32 135 32 466 32 641 32 599
Cuts at: 3342 3374 5053 5085 5127 5159 1373
Size: 32 1679 32 42 32 2626
Fragments arranged by size:
2626 1679 641 599 466 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3039 3756 4002 669
Size: 988 281 1101 717 246 3079
Fragments arranged by size:
3079 1101 988 717 281 246
HindIII A'AGCT_T
Cuts at: 748 3138 748
Size: 2390 4022
HpaI GTT'AAC
Cuts at: 4002 4002
Size: 6412
KpnI G_GTAC'C
Cuts at: 3613 3737 3613
Size: 124 6288
MfeI C'AATT_G
Cuts at: 2738 4011 2738
Size: 1273 5139
MluI A'CGCG_T
Cuts at: 6288 6288
Size: 6412
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5526 5710 1305
Size: 4221 184 2007
Fragments arranged by size:
4221 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4590
Fragments arranged by size:
4590 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2526 2552 2885 3050 4370 4729
Size: 648 843 517 26 333 165 1320 359
Cuts at: 4729 4888 518
Size: 159 2042
Fragments arranged by size:
2042 1320 843 648 517 359 333 165
159 26
MspA1I CmG'CkG
Cuts at: 1606 3390 3546 3828 4505 5653 5898 1606
Size: 1784 156 282 677 1148 245 2120
Fragments arranged by size:
2120 1784 1148 677 282 245 156
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5860
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6412
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6412
NotI GC'GGCC_GC
Cuts at: 3859 3859
Size: 6412
NsiI A_TGCA'T
Cuts at: 2599 2599
Size: 6412
NspI r_CATG'y
Cuts at: 1132 1229 3619 3770 5315 1132
Size: 97 2390 151 1545 2229
Fragments arranged by size:
2390 2229 1545 151 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6412
PciI A'CATG_T
Cuts at: 5311 5311
Size: 6412
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6412
PfoI T'CCnGG_A
Cuts at: 2219 2219
Size: 6412
PmeI GTTT'AAAC
Cuts at: 3716 3716
Size: 6412
PmlI CAC'GTG
Cuts at: 3700 3700
Size: 6412
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4481 4513 5994 6026 4481
Size: 32 1481 32 4867
Fragments arranged by size:
4867 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3574 1613
Size: 1961 4451
PsiI TTA'TAA
Cuts at: 2370 2922 3982 2370
Size: 552 1060 4800
Fragments arranged by size:
4800 1060 552
PstI C_TGCA'G
Cuts at: 830 3764 830
Size: 2934 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6412
PvuII CAG'CTG
Cuts at: 3390 3390
Size: 6412
SalI G'TCGA_C
Cuts at: 1936 3754 1936
Size: 1818 4594
SapI GCTCTTCn'nnn_
Cuts at: 3405 3405
Size: 6412
SbfI CC_TGCA'GG
Cuts at: 3764 3764
Size: 6412
ScaI AGT'ACT
Cuts at: 4660 6372 4660
Size: 1712 4700
SfcI C'TryA_G
Cuts at: 826 1045 2205 3760 4895 5576 5767 826
Size: 219 1160 1555 1135 681 191 1471
Fragments arranged by size:
1555 1471 1160 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6412
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6412
SmaI CCC'GGG
Cuts at: 1872 2636 3739 1872
Size: 764 1103 4545
Fragments arranged by size:
4545 1103 764
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3467 4516 5417 5679 820
Size: 197 926 1524 1049 901 262 1553
Fragments arranged by size:
1553 1524 1049 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4203 493
Size: 3710 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6412
SphI G_CATG'C
Cuts at: 1132 3770 1132
Size: 2638 3774
SspI AAT'ATT
Cuts at: 5 52 4336 5
Size: 47 4284 2081
Fragments arranged by size:
4284 2081 47
StuI AGG'CCT
Cuts at: 1517 2680 1517
Size: 1163 5249
StyI C'CwwG_G
Cuts at: 513 1065 3851 513
Size: 552 2786 3074
Fragments arranged by size:
3074 2786 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3386 4439 4456 4609 4794 5239 325
Size: 1255 1806 1053 17 153 185 445 1498
Fragments arranged by size:
1806 1498 1255 1053 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4658 6370 96
Size: 274 80 33 51 1443 2681 1712 138
Fragments arranged by size:
2681 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2409 2441 3242 3274 6372 6404 2409
Size: 32 801 32 3098 32 2417
Fragments arranged by size:
3098 2417 801 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6412
XbaI T'CTAG_A
Cuts at: 2656 3748 2656
Size: 1092 5320
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6412
XmaI C'CCGG_G
Cuts at: 1870 2634 3737 1870
Size: 764 1103 4545
Fragments arranged by size:
4545 1103 764
XmnI GAAnn'nnTTC
Cuts at: 4541 4541
Size: 6412
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4220 276
Size: 53 83 186 936 2686 2468
Fragments arranged by size:
2686 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PstI PvuI PvuII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI XcmI