Restriction map for
pFN21AB5985
(Circular) (Six-base) MAPSORT of: pFN21AB5985.seq Check: 6451 from: 1 to: 6268
pFN21AB5985 6268 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4078 278
Size: 53 83 186 936 2542 2468
Fragments arranged by size:
2542 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3611 1937
Size: 1674 4594
Acc65I G'GTAC_C
Cuts at: 3589 3589
Size: 6268
AclI AA'CG_TT
Cuts at: 1563 4395 4768 1563
Size: 2832 373 3063
Fragments arranged by size:
3063 2832 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3263 3501 4331 5715 5983 1632
Size: 1631 238 830 1384 268 1917
Fragments arranged by size:
1917 1631 1384 830 268 238
AfeI AGC'GCT
Cuts at: 3215 3215
Size: 6268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6071
AflIII A'CryG_T
Cuts at: 1203 3227 5167 6144 1203
Size: 2024 1940 977 1327
Fragments arranged by size:
2024 1940 1327 977
AgeI A'CCGG_T
Cuts at: 3387 5099 3387
Size: 1712 4556
AhdI GACnn_n'nnGTC
Cuts at: 4997 4997
Size: 6268
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6268
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6268
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3494 3526 3494
Size: 32 6236
AlwNI CAG_nnn'CTG
Cuts at: 2174 2577 2752 4011 5583 2174
Size: 403 175 1259 1572 2859
Fragments arranged by size:
2859 1572 1259 403 175
ApaI G_GGCC'C
Cuts at: 2899 3082 2899
Size: 183 6085
ApaLI G'TGCA_C
Cuts at: 4325 5481 4325
Size: 1156 5112
ApoI r'AATT_y
Cuts at: 1493 3577 3803 3955 6064 1493
Size: 2084 226 152 2109 1697
Fragments arranged by size:
2109 2084 1697 226 152
AscI GG'CGCG_CC
Cuts at: 3374 3374
Size: 6268
AseI AT'TA_AT
Cuts at: 160 1032 4822 160
Size: 872 3790 1606
Fragments arranged by size:
3790 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6268
AvaI C'yCGr_G
Cuts at: 1870 1943 2023 2039 2899 3068 3318 3593 1870
Size: 73 80 16 860 169 250 275 4545
Fragments arranged by size:
4545 860 275 250 169 80 73 16
BamHI G'GATC_C
Cuts at: 3598 3965 3598
Size: 367 5901
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2366 3589 5044 618
Size: 325 217 627 579 1223 1455 1842
Fragments arranged by size:
1842 1455 1223 627 579 325 217
BanII G_rGCy'C
Cuts at: 1391 2048 2261 2899 3018 3082 3587 1391
Size: 657 213 638 119 64 505 4072
Fragments arranged by size:
4072 657 638 505 213 119 64
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5297
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4459 4493 1903
Size: 34 2522 34 3678
Fragments arranged by size:
3678 2522 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4160 5370 4160
Size: 1210 5058
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6268
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2894 4879 136
Size: 107 122 71 631 605 1222 1985 1525
Fragments arranged by size:
1985 1525 1222 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6263 6263
Size: 6268
BlpI GC'TnA_GC
Cuts at: 2403 3685 2403
Size: 1282 4986
Bme1580I G_kGCm'C
Cuts at: 1790 2899 3082 3319 4329 5485 1790
Size: 1109 183 237 1010 1156 2573
Fragments arranged by size:
2573 1156 1109 1010 237 183
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6268
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2565 2902 3999
Size: 416 53 83 399 24 1148 337 1097
Cuts at: 3999 4957 442
Size: 958 1753
Fragments arranged by size:
1753 1148 1097 958 416 399 337 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6268
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2128 2599 3386 3521 4928 1239
Size: 889 471 787 135 1407 2579
Fragments arranged by size:
2579 1407 889 787 471 135
Bpu10I CC'TnA_GC
Cuts at: 3049 3086 3049
Size: 37 6231
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2136 2975 3134 4393 5258 5556 2136
Size: 839 159 1259 865 298 2848
Fragments arranged by size:
2848 1259 865 839 298 159
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2171 2321 4931 882
Size: 617 672 150 2610 2219
Fragments arranged by size:
2610 2219 672 617 150
BsaAI yAC'GTr
Cuts at: 493 1204 3184 3228 4059 493
Size: 711 1980 44 831 2702
Fragments arranged by size:
2702 1980 831 711 44
BsaBI GATnn'nnATC
Cuts at: 2666 6262 2666
Size: 3596 2672
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2881 3285 3330 4075
Size: 53 83 186 936 1348 404 45 745
Cuts at: 4075 4457 5092 275
Size: 382 635 1451
Fragments arranged by size:
1451 1348 936 745 635 404 382 186
83 53 45
BsaWI w'CCGG_w
Cuts at: 2133 2778 3387 4701 5099 5373 5520 5931 2133
Size: 645 609 1314 398 274 147 411 2470
Fragments arranged by size:
2470 1314 645 609 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3494 3524 1178
Size: 30 165 30 2091 30 3922
Fragments arranged by size:
3922 2091 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2708 2993 3400 1186
Size: 1522 285 407 4054
Fragments arranged by size:
4054 1522 407 285
BseYI C'CCAG_C
Cuts at: 2203 2319 2346 2442 2480 2731 3019 3535 5471
Size: 116 27 96 38 251 288 516 1936
Cuts at: 5471 5967 2203
Size: 496 2504
Fragments arranged by size:
2504 1936 516 496 288 251 116 96
38 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5695
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2935 3171 3718 4479 5507 1540
Size: 275 186 934 236 547 761 1028 2301
Fragments arranged by size:
2301 1028 934 761 547 275 236 186
BsiHKAI G_wGCw'C
Cuts at: 2144 3018 4329 4414 5485 2144
Size: 874 1311 85 1071 2927
Fragments arranged by size:
2927 1311 1071 874 85
BsmI GAATG_Cn'
Cuts at: 3779 3872 3779
Size: 93 6175
BsmBI CGTCTCn'nnnn_
Cuts at: 2862 2862
Size: 6268
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2048 2144 2261 2899 3018 3082 3319
Size: 399 258 96 117 638 119 64 237
Cuts at: 3319 3587 4329 4414 5485 1391
Size: 268 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 638 399 268 258 237
119 117 96 85 64
BspEI T'CCGG_A
Cuts at: 5931 5931
Size: 6268
BspHI T'CATG_A
Cuts at: 4157 5887 4157
Size: 1730 4538
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3623 844
Size: 2779 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3048 4155 1394
Size: 429 1225 1107 3507
Fragments arranged by size:
3507 1225 1107 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4763 4937 65
Size: 1183 3515 174 1396
Fragments arranged by size:
3515 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2785 3005 3387 4912 5099 1700
Size: 110 975 220 382 1525 187 2869
Fragments arranged by size:
2869 1525 975 382 220 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6268
BssHII G'CGCG_C
Cuts at: 1925 3374 1925
Size: 1449 4819
BssSI C'ACGA_G
Cuts at: 3161 4328 5340 3161
Size: 1167 1012 4089
Fragments arranged by size:
4089 1167 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6268
BstBI TT'CG_AA
Cuts at: 5072 5072
Size: 6268
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6268
BstYI r'GATC_y
Cuts at: 1972 2170 2583 3504 3598 3965 4350 4367 5819
Size: 198 413 921 94 367 385 17 1452
Cuts at: 5819 5905 5917 6263 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 921 413 385 367 346 198
94 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2976 2976
Size: 6268
BtgI C'CryG_G
Cuts at: 513 1065 2004 3324 513
Size: 552 939 1320 3457
Fragments arranged by size:
3457 1320 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5529
Fragments arranged by size:
5529 683 56
BtsI GCAGTG_nn'
Cuts at: 2199 3120 3784 4578 4598 2199
Size: 921 664 794 20 3869
Fragments arranged by size:
3869 921 794 664 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6268
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6233
DraI TTT'AAA
Cuts at: 3572 3572
Size: 6268
DraIII CAC_nnn'GTG
Cuts at: 3163 3163
Size: 6268
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5275 809
Size: 507 3959 1802
Fragments arranged by size:
3959 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2757 2886 2996
Size: 54 1425 50 275 18 927 129 110
Cuts at: 2996 3168 3715 4604 6233 8
Size: 172 547 889 1629 43
Fragments arranged by size:
1629 1425 927 889 547 275 172 129
110 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3168 3715 1537
Size: 275 1356 547 4090
Fragments arranged by size:
4090 1356 547 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2319 2387 3356 4198 1353
Size: 966 68 969 842 3423
Fragments arranged by size:
3423 969 966 842 68
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2349 2920 4855 5229 5375 387
Size: 1064 138 760 571 1935 374 146 1280
Fragments arranged by size:
1935 1280 1064 760 571 374 146 138
EcoICRI GAG'CTC
Cuts at: 3016 3016
Size: 6268
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2128 2599 3263 3386 3501 3521 4331
Size: 393 496 471 664 123 115 20 810
Cuts at: 4331 4928 5715 5983 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 810 787 664 597 496 471 393
268 123 115 20
EcoNI CCTnn'n_nnAGG
Cuts at: 2120 2120
Size: 6268
EcoO109I rG'GnC_Cy
Cuts at: 2020 2111 2229 2737 2896 3053 3078 3079 3466
Size: 91 118 508 159 157 25 1 387
Cuts at: 3466 2020
Size: 4822
Fragments arranged by size:
4822 508 387 159 157 118 91 25
1
EcoRI G'AATT_C
Cuts at: 3577 3955 3577
Size: 378 5890
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2935 3160 2935
Size: 225 6043
FspI TGC'GCA
Cuts at: 4774 4774
Size: 6268
HaeII r_GCGC'y
Cuts at: 1678 2063 3093 3217 5415 1678
Size: 385 1030 124 2198 2531
Fragments arranged by size:
2531 2198 1030 385 124
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4909 4941 4983 5015 1373
Size: 32 135 32 3337 32 42 32 2626
Fragments arranged by size:
3337 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2212 3612 3858 669
Size: 988 281 274 1400 246 3079
Fragments arranged by size:
3079 1400 988 281 274 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6268
HpaI GTT'AAC
Cuts at: 2212 3858 2212
Size: 1646 4622
KpnI G_GTAC'C
Cuts at: 3593 3593
Size: 6268
MfeI C'AATT_G
Cuts at: 3867 3867
Size: 6268
MluI A'CGCG_T
Cuts at: 6144 6144
Size: 6268
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2232 5382 5566 1305
Size: 927 3150 184 2007
Fragments arranged by size:
3150 2007 927 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2759 2998 10
Size: 54 1425 343 927 239 3280
Fragments arranged by size:
3280 1425 927 343 239 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2861 3437 4226 4585 4744 518
Size: 648 1695 576 789 359 159 2042
Fragments arranged by size:
2042 1695 789 648 576 359 159
MspA1I CmG'CkG
Cuts at: 1606 2203 2480 3326 3342 3684 4361 5509 5754
Size: 597 277 846 16 342 677 1148 245
Cuts at: 5754 1606
Size: 2120
Fragments arranged by size:
2120 1148 846 677 597 342 277 245
16
NaeI GCC'GGC
Cuts at: 3007 3007
Size: 6268
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4777
Fragments arranged by size:
4777 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6268
NgoMIV G'CCGG_C
Cuts at: 3005 3005
Size: 6268
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6268
NotI GC'GGCC_GC
Cuts at: 3715 3715
Size: 6268
NspI r_CATG'y
Cuts at: 1132 1229 2800 3626 5171 1132
Size: 97 1571 826 1545 2229
Fragments arranged by size:
2229 1571 1545 826 97
PasI CC'CwG_GG
Cuts at: 1348 2051 2494 3125 1348
Size: 703 443 631 4491
Fragments arranged by size:
4491 703 631 443
PciI A'CATG_T
Cuts at: 5167 5167
Size: 6268
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2577 2752 1108
Size: 1469 175 4624
Fragments arranged by size:
4624 1469 175
PfoI T'CCnGG_A
Cuts at: 3498 3498
Size: 6268
PmeI GTTT'AAAC
Cuts at: 3572 3572
Size: 6268
PmlI CAC'GTG
Cuts at: 3184 3228 3184
Size: 44 6224
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3494 3526 4337 4369 5850 5882 3494
Size: 32 811 32 1481 32 3880
Fragments arranged by size:
3880 1481 811 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2111 2229 2737 2111
Size: 118 508 5642
Fragments arranged by size:
5642 508 118
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6268
PsiI TTA'TAA
Cuts at: 3838 3838
Size: 6268
PspOMI G'GGCC_C
Cuts at: 2895 3078 2895
Size: 183 6085
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3539 3571 3539
Size: 32 6236
PstI C_TGCA'G
Cuts at: 830 2814 3620 830
Size: 1984 806 3478
Fragments arranged by size:
3478 1984 806
PvuI CG_AT'CG
Cuts at: 2001 2935 2001
Size: 934 5334
PvuII CAG'CTG
Cuts at: 2203 2480 2203
Size: 277 5991
RsrII CG'GwC_CG
Cuts at: 2131 2131
Size: 6268
SacI G_AGCT'C
Cuts at: 3018 3018
Size: 6268
SacII CC_GC'GG
Cuts at: 3327 3327
Size: 6268
SalI G'TCGA_C
Cuts at: 1936 3610 1936
Size: 1674 4594
SanDI GG'GwC_CC
Cuts at: 2111 2229 2737 2111
Size: 118 508 5642
Fragments arranged by size:
5642 508 118
SbfI CC_TGCA'GG
Cuts at: 3620 3620
Size: 6268
ScaI AGT'ACT
Cuts at: 4516 6228 4516
Size: 1712 4556
SfcI C'TryA_G
Cuts at: 826 1045 2810 3448 3616 4751 5432 5623 826
Size: 219 1765 638 168 1135 681 191 1471
Fragments arranged by size:
1765 1471 1135 681 638 219 191 168
SfiI GGCCn_nnn'nGGCC
Cuts at: 2894 2894
Size: 6268
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6268
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6268
SmaI CCC'GGG
Cuts at: 1872 2025 2901 3595 1872
Size: 153 876 694 4545
Fragments arranged by size:
4545 876 694 153
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2039 2151 2990 3149 4372 5273
Size: 197 926 96 112 839 159 1223 901
Cuts at: 5273 5535 820
Size: 262 1553
Fragments arranged by size:
1553 1223 926 901 839 262 197 159
112 96
SnaBI TAC'GTA
Cuts at: 493 4059 493
Size: 3566 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6268
SphI G_CATG'C
Cuts at: 1132 3626 1132
Size: 2494 3774
SspI AAT'ATT
Cuts at: 5 52 4192 5
Size: 47 4140 2081
Fragments arranged by size:
4140 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6268
StyI C'CwwG_G
Cuts at: 513 1065 2004 2706 2953 3707 513
Size: 552 939 702 247 754 3074
Fragments arranged by size:
3074 939 754 702 552 247
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2319 3511 4295 4312 4465 4650 5095
Size: 1255 739 1192 784 17 153 185 445
Cuts at: 5095 325
Size: 1498
Fragments arranged by size:
1498 1255 1192 784 739 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3546 4514 6226
Size: 274 80 33 51 1443 1569 968 1712
Cuts at: 6226 96
Size: 138
Fragments arranged by size:
1712 1569 1443 968 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2123 2155 6228 6260 2123
Size: 32 4073 32 2131
Fragments arranged by size:
4073 2131 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3434 1316
Size: 2118 4150
XbaI T'CTAG_A
Cuts at: 3604 3604
Size: 6268
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2152 3100 2011
Size: 141 948 5179
Fragments arranged by size:
5179 948 141
XhoI C'TCGA_G
Cuts at: 1943 2039 1943
Size: 96 6172
XmaI C'CCGG_G
Cuts at: 1870 2023 2899 3593 1870
Size: 153 876 694 4545
Fragments arranged by size:
4545 876 694 153
XmnI GAAnn'nnTTC
Cuts at: 2918 3458 4397 2918
Size: 540 939 4789
Fragments arranged by size:
4789 939 540
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4076 276
Size: 53 83 186 936 2542 2468
Fragments arranged by size:
2542 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI
BstXI BstZ17I EcoRV FseI FspAI KasI NarI NruI
NsiI PacI PspXI SapI SexAI SfoI SrfI SwaI