Restriction map for
pFN21AB5983
(Circular) (Six-base) MAPSORT of: pFN21AB5983.seq Check: 9364 from: 1 to: 6268
pFN21AB5983 6268 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4078 278
Size: 53 83 186 936 2542 2468
Fragments arranged by size:
2542 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3611 1937
Size: 1674 4594
Acc65I G'GTAC_C
Cuts at: 3589 3589
Size: 6268
AclI AA'CG_TT
Cuts at: 1563 4395 4768 1563
Size: 2832 373 3063
Fragments arranged by size:
3063 2832 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2379 2484 2556 2564 3455 4331 5715 5983
Size: 747 105 72 8 891 876 1384 268
Cuts at: 5983 1632
Size: 1917
Fragments arranged by size:
1917 1384 891 876 747 268 105 72
8
AfeI AGC'GCT
Cuts at: 2312 3100 2312
Size: 788 5480
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6071
AflIII A'CryG_T
Cuts at: 1203 5167 6144 1203
Size: 3964 977 1327
Fragments arranged by size:
3964 1327 977
AgeI A'CCGG_T
Cuts at: 2990 5099 2990
Size: 2109 4159
AhdI GACnn_n'nnGTC
Cuts at: 4997 4997
Size: 6268
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6268
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6268
AlwNI CAG_nnn'CTG
Cuts at: 2190 3046 4011 5583 2190
Size: 856 965 1572 2875
Fragments arranged by size:
2875 1572 965 856
ApaLI G'TGCA_C
Cuts at: 4325 5481 4325
Size: 1156 5112
ApoI r'AATT_y
Cuts at: 1493 2027 2033 2870 3577 3803 3955 6064 1493
Size: 534 6 837 707 226 152 2109 1697
Fragments arranged by size:
2109 1697 837 707 534 226 152 6
AseI AT'TA_AT
Cuts at: 160 1032 4822 160
Size: 872 3790 1606
Fragments arranged by size:
3790 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6268
AvaI C'yCGr_G
Cuts at: 1870 1943 3555 3593 1870
Size: 73 1612 38 4545
Fragments arranged by size:
4545 1612 73 38
BamHI G'GATC_C
Cuts at: 3598 3965 3598
Size: 367 5901
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3589 5044 618
Size: 325 217 627 1802 1455 1842
Fragments arranged by size:
1842 1802 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3587 1391
Size: 2196 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3182 928
Size: 971 1283 4014
Fragments arranged by size:
4014 1283 971
BbvCI CC'TCA_GC
Cuts at: 3108 3108
Size: 6268
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4459 4493 1903
Size: 34 2522 34 3678
Fragments arranged by size:
3678 2522 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4160 5370 4160
Size: 1210 5058
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6268
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3530 4879 136
Size: 107 122 71 631 605 1858 1349 1525
Fragments arranged by size:
1858 1525 1349 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6263 6263
Size: 6268
BlpI GC'TnA_GC
Cuts at: 3024 3685 3024
Size: 661 5607
Bme1580I G_kGCm'C
Cuts at: 1790 3364 4329 5485 1790
Size: 1574 965 1156 2573
Fragments arranged by size:
2573 1574 1156 965
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6268
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2928 3999 4957
Size: 416 53 83 399 24 1511 1071 958
Cuts at: 4957 442
Size: 1753
Fragments arranged by size:
1753 1511 1071 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6268
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2944 3307 2944
Size: 363 5905
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2576 4928 1239
Size: 1337 2352 2579
Fragments arranged by size:
2579 2352 1337
Bpu10I CC'TnA_GC
Cuts at: 3108 3108
Size: 6268
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4393 5258 5556 4393
Size: 865 298 5105
Fragments arranged by size:
5105 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4931 882
Size: 617 3432 2219
Fragments arranged by size:
3432 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4059 493
Size: 711 2855 2702
Fragments arranged by size:
2855 2702 711
BsaBI GATnn'nnATC
Cuts at: 2238 6262 2238
Size: 4024 2244
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4075 4457 5092 275
Size: 53 83 186 936 2542 382 635 1451
Fragments arranged by size:
2542 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2990 4701 5099 5373 5520 5931 2990
Size: 1711 398 274 147 411 3327
Fragments arranged by size:
3327 1711 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6043
Fragments arranged by size:
6043 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2302 1186
Size: 1116 5152
BseYI C'CCAG_C
Cuts at: 2190 2740 5471 5967 2190
Size: 550 2731 496 2491
Fragments arranged by size:
2731 2491 550 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3146 1163
Size: 573 1410 4285
Fragments arranged by size:
4285 1410 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3718 4479 5507 1540
Size: 275 186 1717 761 1028 2301
Fragments arranged by size:
2301 1717 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2294 4329 4414 5485 2294
Size: 2035 85 1071 3077
Fragments arranged by size:
3077 2035 1071 85
BsmI GAATG_Cn'
Cuts at: 3779 3872 3779
Size: 93 6175
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2294 3364 3587 4329 4414 5485 1391
Size: 399 504 1070 223 742 85 1071 2174
Fragments arranged by size:
2174 1071 1070 742 504 399 223 85
BspEI T'CCGG_A
Cuts at: 5931 5931
Size: 6268
BspHI T'CATG_A
Cuts at: 4157 5887 4157
Size: 1730 4538
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2307 3623 844
Size: 1463 1316 3489
Fragments arranged by size:
3489 1463 1316
BsrBI CCG'CTC
Cuts at: 1394 1823 4155 1394
Size: 429 2332 3507
Fragments arranged by size:
3507 2332 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3035 4763 4937 65
Size: 1183 1787 1728 174 1396
Fragments arranged by size:
1787 1728 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2990 3120 4912 5099 1700
Size: 110 1180 130 1792 187 2869
Fragments arranged by size:
2869 1792 1180 187 130 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6268
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6268
BssSI C'ACGA_G
Cuts at: 4328 5340 4328
Size: 1012 5256
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6268
BstBI TT'CG_AA
Cuts at: 5072 5072
Size: 6268
BstEII G'GTnAC_C
Cuts at: 1184 3326 1184
Size: 2142 4126
BstXI CCAn_nnnn'nTGG
Cuts at: 2174 2174
Size: 6268
BstYI r'GATC_y
Cuts at: 1972 2148 3598 3965 4350 4367 5819 5905 5917
Size: 176 1450 367 385 17 1452 86 12
Cuts at: 5917 6263 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1450 385 367 346 176 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2285 2285
Size: 6268
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5716
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5529
Fragments arranged by size:
5529 683 56
BtsI GCAGTG_nn'
Cuts at: 3784 4578 4598 3784
Size: 794 20 5454
Fragments arranged by size:
5454 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6268
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6233
DraI TTT'AAA
Cuts at: 2032 3572 2032
Size: 1540 4728
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5275 809
Size: 507 3959 1802
Fragments arranged by size:
3959 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3715 4604 6233
Size: 54 1425 50 275 18 1885 889 1629
Cuts at: 6233 8
Size: 43
Fragments arranged by size:
1885 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3715 1537
Size: 275 1903 4090
Fragments arranged by size:
4090 1903 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3161 4198 1353
Size: 1808 1037 3423
Fragments arranged by size:
3423 1808 1037
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2928 3127 4855 5229 5375 387
Size: 1064 138 1339 199 1728 374 146 1280
Fragments arranged by size:
1728 1339 1280 1064 374 199 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2379 2484 2556 2564 2576 3455 4331
Size: 393 747 105 72 8 12 879 876
Cuts at: 4331 4928 5715 5983 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 879 876 787 747 597 393 268
105 72 12 8
EcoNI CCTnn'n_nnAGG
Cuts at: 2765 2765
Size: 6268
EcoO109I rG'GnC_Cy
Cuts at: 3286 3286
Size: 6268
EcoRI G'AATT_C
Cuts at: 3577 3955 3577
Size: 378 5890
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3433 3433
Size: 6268
FspI TGC'GCA
Cuts at: 4774 4774
Size: 6268
HaeII r_GCGC'y
Cuts at: 1678 2314 3102 3121 5415 1678
Size: 636 788 19 2294 2531
Fragments arranged by size:
2531 2294 788 636 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2369 2401 2677 2709 4909
Size: 32 135 32 797 32 276 32 2200
Cuts at: 4909 4941 4983 5015 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2200 797 276 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2758 3612 3858 669
Size: 988 281 820 854 246 3079
Fragments arranged by size:
3079 988 854 820 281 246
HindIII A'AGCT_T
Cuts at: 748 3299 748
Size: 2551 3717
HpaI GTT'AAC
Cuts at: 3858 3858
Size: 6268
KpnI G_GTAC'C
Cuts at: 3593 3593
Size: 6268
MfeI C'AATT_G
Cuts at: 3867 3867
Size: 6268
MluI A'CGCG_T
Cuts at: 6144 6144
Size: 6268
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2159 5382 5566 1305
Size: 854 3223 184 2007
Fragments arranged by size:
3223 2007 854 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4446
Fragments arranged by size:
4446 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2172 2726 4226 4585 4744 518
Size: 648 1006 554 1500 359 159 2042
Fragments arranged by size:
2042 1500 1006 648 554 359 159
MspA1I CmG'CkG
Cuts at: 1606 2554 3684 4361 5509 5754 1606
Size: 948 1130 677 1148 245 2120
Fragments arranged by size:
2120 1148 1130 948 677 245
NaeI GCC'GGC
Cuts at: 3122 3122
Size: 6268
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5716
NdeI CA'TA_TG
Cuts at: 387 2833 387
Size: 2446 3822
NgoMIV G'CCGG_C
Cuts at: 3120 3120
Size: 6268
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6268
NotI GC'GGCC_GC
Cuts at: 3715 3715
Size: 6268
NspI r_CATG'y
Cuts at: 1132 1229 2717 3148 3626 5171 1132
Size: 97 1488 431 478 1545 2229
Fragments arranged by size:
2229 1545 1488 478 431 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6268
PciI A'CATG_T
Cuts at: 5167 5167
Size: 6268
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6268
PfoI T'CCnGG_A
Cuts at: 3293 3293
Size: 6268
PmeI GTTT'AAAC
Cuts at: 3572 3572
Size: 6268
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4337 4369 5850 5882 4337
Size: 32 1481 32 4723
Fragments arranged by size:
4723 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3286 3286
Size: 6268
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6268
PsiI TTA'TAA
Cuts at: 3838 3838
Size: 6268
PstI C_TGCA'G
Cuts at: 830 2318 3620 830
Size: 1488 1302 3478
Fragments arranged by size:
3478 1488 1302
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6268
PvuII CAG'CTG
Cuts at: 2554 2554
Size: 6268
SalI G'TCGA_C
Cuts at: 1936 3610 1936
Size: 1674 4594
SapI GCTCTTCn'nnn_
Cuts at: 3161 3161
Size: 6268
SbfI CC_TGCA'GG
Cuts at: 3620 3620
Size: 6268
ScaI AGT'ACT
Cuts at: 4516 6228 4516
Size: 1712 4556
SfcI C'TryA_G
Cuts at: 826 1045 2274 2314 2407 2523 3616 4751 5432
Size: 219 1229 40 93 116 1093 1135 681
Cuts at: 5432 5623 826
Size: 191 1471
Fragments arranged by size:
1471 1229 1135 1093 681 219 191 116
93 40
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6268
SgrAI Cr'CCGG_yG
Cuts at: 1700 2990 1700
Size: 1290 4978
SmaI CCC'GGG
Cuts at: 1872 3557 3595 1872
Size: 1685 38 4545
Fragments arranged by size:
4545 1685 38
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4372 5273 5535 820
Size: 197 926 2429 901 262 1553
Fragments arranged by size:
2429 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4059 493
Size: 3566 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6268
SphI G_CATG'C
Cuts at: 1132 3148 3626 1132
Size: 2016 478 3774
Fragments arranged by size:
3774 2016 478
SspI AAT'ATT
Cuts at: 5 52 4192 5
Size: 47 4140 2081
Fragments arranged by size:
4140 2081 47
StuI AGG'CCT
Cuts at: 1517 2349 2689 1517
Size: 832 340 5096
Fragments arranged by size:
5096 832 340
StyI C'CwwG_G
Cuts at: 513 1065 2079 3403 3707 513
Size: 552 1014 1324 304 3074
Fragments arranged by size:
3074 1324 1014 552 304
SwaI ATTT'AAAT
Cuts at: 2032 2032
Size: 6268
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4295 4312 4465 4650 5095 325
Size: 1255 2715 17 153 185 445 1498
Fragments arranged by size:
2715 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4514 6226 96
Size: 274 80 33 51 1443 2537 1712 138
Fragments arranged by size:
2537 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6228 6260 6228
Size: 32 6236
Tth111I GACn'n_nGTC
Cuts at: 1316 2336 2863 1316
Size: 1020 527 4721
Fragments arranged by size:
4721 1020 527
XbaI T'CTAG_A
Cuts at: 3604 3604
Size: 6268
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6268
XmaI C'CCGG_G
Cuts at: 1870 3555 3593 1870
Size: 1685 38 4545
Fragments arranged by size:
4545 1685 38
XmnI GAAnn'nnTTC
Cuts at: 4397 4397
Size: 6268
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4076 276
Size: 53 83 186 936 2542 2468
Fragments arranged by size:
2542 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI
KasI NarI NruI NsiI PacI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI XcmI