Restriction map for
pFN21AB5982
(Circular) (Six-base) MAPSORT of: pFN21AB5982.seq Check: 6011 from: 1 to: 6409
pFN21AB5982 6409 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4219 278
Size: 53 83 186 936 2683 2468
Fragments arranged by size:
2683 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3752 1937
Size: 1815 4594
Acc65I G'GTAC_C
Cuts at: 3730 3730
Size: 6409
AclI AA'CG_TT
Cuts at: 1563 4536 4909 1563
Size: 2973 373 3063
Fragments arranged by size:
3063 2973 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2327 2514 2601 4472 5856 6124 1632
Size: 695 187 87 1871 1384 268 1917
Fragments arranged by size:
1917 1871 1384 695 268 187 87
AfeI AGC'GCT
Cuts at: 3197 3674 3197
Size: 477 5932
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6212
AflIII A'CryG_T
Cuts at: 1203 2293 5308 6285 1203
Size: 1090 3015 977 1327
Fragments arranged by size:
3015 1327 1090 977
AgeI A'CCGG_T
Cuts at: 5240 5240
Size: 6409
AhdI GACnn_n'nnGTC
Cuts at: 5138 5138
Size: 6409
AleI CACnn'nnGTG
Cuts at: 1166 2442 1166
Size: 1276 5133
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6409
AlwNI CAG_nnn'CTG
Cuts at: 4152 5724 4152
Size: 1572 4837
ApaLI G'TGCA_C
Cuts at: 4466 5622 4466
Size: 1156 5253
ApoI r'AATT_y
Cuts at: 1493 2259 2565 3325 3718 3944 4096 6205 1493
Size: 766 306 760 393 226 152 2109 1697
Fragments arranged by size:
2109 1697 766 760 393 306 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4963 160
Size: 872 3931 1606
Fragments arranged by size:
3931 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6409
AvaI C'yCGr_G
Cuts at: 1870 1943 3249 3734 1870
Size: 73 1306 485 4545
Fragments arranged by size:
4545 1306 485 73
AvrII C'CTAG_G
Cuts at: 2833 2833
Size: 6409
BamHI G'GATC_C
Cuts at: 3739 4106 3739
Size: 367 6042
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3257 3730 5185 618
Size: 325 217 627 1470 473 1455 1842
Fragments arranged by size:
1842 1470 1455 627 473 325 217
BanII G_rGCy'C
Cuts at: 1391 2202 2830 3728 1391
Size: 811 628 898 4072
Fragments arranged by size:
4072 898 811 628
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5438
BbvCI CC'TCA_GC
Cuts at: 2466 2466
Size: 6409
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4600 4634 1903
Size: 34 2663 34 3678
Fragments arranged by size:
3678 2663 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4301 5511 4301
Size: 1210 5199
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6409
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5020 136
Size: 107 122 71 631 605 3348 1525
Fragments arranged by size:
3348 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6404 6404
Size: 6409
BlpI GC'TnA_GC
Cuts at: 2718 3826 2718
Size: 1108 5301
Bme1580I G_kGCm'C
Cuts at: 1790 4470 5626 1790
Size: 2680 1156 2573
Fragments arranged by size:
2680 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6409
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2620 4140 5098
Size: 416 53 83 399 24 1203 1520 958
Cuts at: 5098 442
Size: 1753
Fragments arranged by size:
1753 1520 1203 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6409
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3141 3177 5069 1239
Size: 1902 36 1892 2579
Fragments arranged by size:
2579 1902 1892 36
Bpu10I CC'TnA_GC
Cuts at: 2466 2466
Size: 6409
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4534 5399 5697 4534
Size: 865 298 5246
Fragments arranged by size:
5246 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5072 882
Size: 617 3573 2219
Fragments arranged by size:
3573 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4200 493
Size: 711 2996 2702
Fragments arranged by size:
2996 2702 711
BsaBI GATnn'nnATC
Cuts at: 6403 6403
Size: 6409
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3178 4216 4598 5233
Size: 53 83 186 936 1645 1038 382 635
Cuts at: 5233 275
Size: 1451
Fragments arranged by size:
1645 1451 1038 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2991 4842 5240 5514 5661 6072 2991
Size: 1851 398 274 147 411 3328
Fragments arranged by size:
3328 1851 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6184
Fragments arranged by size:
6184 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6409
BseYI C'CCAG_C
Cuts at: 3229 3642 3669 5612 6108 3229
Size: 413 27 1943 496 3530
Fragments arranged by size:
3530 1943 496 413 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3420 1163
Size: 573 1684 4152
Fragments arranged by size:
4152 1684 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3859 4620 5648 1540
Size: 275 186 1858 761 1028 2301
Fragments arranged by size:
2301 1858 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2202 4470 4555 5626 2202
Size: 2268 85 1071 2985
Fragments arranged by size:
2985 2268 1071 85
BsmI GAATG_Cn'
Cuts at: 3920 4013 3920
Size: 93 6316
BsmBI CGTCTCn'nnnn_
Cuts at: 2953 2953
Size: 6409
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2202 2830 3728 4470 4555 5626 1391
Size: 399 412 628 898 742 85 1071 2174
Fragments arranged by size:
2174 1071 898 742 628 412 399 85
BspEI T'CCGG_A
Cuts at: 6072 6072
Size: 6409
BspHI T'CATG_A
Cuts at: 2986 4298 6028 2986
Size: 1312 1730 3367
Fragments arranged by size:
3367 1730 1312
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2664 3764 844
Size: 1820 1100 3489
Fragments arranged by size:
3489 1820 1100
BsrBI CCG'CTC
Cuts at: 1394 1823 2635 4296 1394
Size: 429 812 1661 3507
Fragments arranged by size:
3507 1661 812 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4904 5078 65
Size: 1183 3656 174 1396
Fragments arranged by size:
3656 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5053 5240 1700
Size: 110 3243 187 2869
Fragments arranged by size:
3243 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2277 2311 96
Size: 2181 34 4194
Fragments arranged by size:
4194 2181 34
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6409
BssSI C'ACGA_G
Cuts at: 4469 5481 4469
Size: 1012 5397
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6409
BstBI TT'CG_AA
Cuts at: 5213 5213
Size: 6409
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6409
BstXI CCAn_nnnn'nTGG
Cuts at: 2896 3133 2896
Size: 237 6172
BstYI r'GATC_y
Cuts at: 1972 2752 2994 3739 4106 4491 4508 5960 6046
Size: 780 242 745 367 385 17 1452 86
Cuts at: 6046 6058 6404 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 780 745 385 367 346 242
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2081 2081
Size: 6409
BtgI C'CryG_G
Cuts at: 513 1065 2004 2253 3660 513
Size: 552 939 249 1407 3262
Fragments arranged by size:
3262 1407 939 552 249
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5670
Fragments arranged by size:
5670 683 56
BtsI GCAGTG_nn'
Cuts at: 3925 4719 4739 3925
Size: 794 20 5595
Fragments arranged by size:
5595 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6409
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6374
DraI TTT'AAA
Cuts at: 3713 3713
Size: 6409
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5416 809
Size: 507 4100 1802
Fragments arranged by size:
4100 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3019 3273 3381
Size: 54 1425 50 275 18 1189 254 108
Cuts at: 3381 3856 4745 6374 8
Size: 475 889 1629 43
Fragments arranged by size:
1629 1425 1189 889 475 275 254 108
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3856 1537
Size: 275 2044 4090
Fragments arranged by size:
4090 2044 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2081 2505 2577 4339 1353
Size: 728 424 72 1762 3423
Fragments arranged by size:
3423 1762 728 424 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4996 5370 5516 387
Size: 1064 138 3407 374 146 1280
Fragments arranged by size:
3407 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2200 2200
Size: 6409
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2327 2514 2601 3141 3177 4472 5069
Size: 393 695 187 87 540 36 1295 597
Cuts at: 5069 5856 6124 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1295 787 695 597 540 393 268
187 87 36
EcoNI CCTnn'n_nnAGG
Cuts at: 3351 3351
Size: 6409
EcoO109I rG'GnC_Cy
Cuts at: 2813 2813
Size: 6409
EcoRI G'AATT_C
Cuts at: 3718 4096 3718
Size: 378 6031
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2339 2339
Size: 6409
FspI TGC'GCA
Cuts at: 4915 4915
Size: 6409
HaeII r_GCGC'y
Cuts at: 1678 3199 3676 5556 1678
Size: 1521 477 1880 2531
Fragments arranged by size:
2531 1880 1521 477
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2029 2061 2812 2844 3129
Size: 32 135 32 457 32 751 32 285
Cuts at: 3129 3161 3277 3309 5050 5082 5124 5156 1373
Size: 32 116 32 1741 32 42 32 2626
Fragments arranged by size:
2626 1741 751 457 285 135 116 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2227 2449 3753 3999 669
Size: 988 281 289 222 1304 246 3079
Fragments arranged by size:
3079 1304 988 289 281 246 222
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6409
HpaI GTT'AAC
Cuts at: 3999 3999
Size: 6409
KpnI G_GTAC'C
Cuts at: 3734 3734
Size: 6409
MfeI C'AATT_G
Cuts at: 2440 3576 4008 2440
Size: 1136 432 4841
Fragments arranged by size:
4841 1136 432
MluI A'CGCG_T
Cuts at: 6285 6285
Size: 6409
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2373 3130 5523 5707 1305
Size: 1068 757 2393 184 2007
Fragments arranged by size:
2393 2007 1068 757 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3275 3383 10
Size: 54 1425 343 1443 108 3036
Fragments arranged by size:
3036 1443 1425 343 108 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2442 2894 3188 3429 3518 4367 4726
Size: 648 1276 452 294 241 89 849 359
Cuts at: 4726 4885 518
Size: 159 2042
Fragments arranged by size:
2042 1276 849 648 452 359 294 241
159 89
MspA1I CmG'CkG
Cuts at: 1606 2465 2733 3642 3825 4502 5650 5895 1606
Size: 859 268 909 183 677 1148 245 2120
Fragments arranged by size:
2120 1148 909 859 677 268 245 183
NcoI C'CATG_G
Cuts at: 513 1065 2004 2253 3660 513
Size: 552 939 249 1407 3262
Fragments arranged by size:
3262 1407 939 552 249
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6409
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6409
NotI GC'GGCC_GC
Cuts at: 3856 3856
Size: 6409
NspI r_CATG'y
Cuts at: 1132 1229 2297 2624 3229 3767 5312 1132
Size: 97 1068 327 605 538 1545 2229
Fragments arranged by size:
2229 1545 1068 605 538 327 97
PasI CC'CwG_GG
Cuts at: 1348 3133 1348
Size: 1785 4624
PciI A'CATG_T
Cuts at: 2293 5308 2293
Size: 3015 3394
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6409
PfoI T'CCnGG_A
Cuts at: 3500 3500
Size: 6409
PmeI GTTT'AAAC
Cuts at: 3713 3713
Size: 6409
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4478 4510 5991 6023 4478
Size: 32 1481 32 4864
Fragments arranged by size:
4864 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2813 2813
Size: 6409
PshAI GACnn'nnGTC
Cuts at: 1613 3509 1613
Size: 1896 4513
PsiI TTA'TAA
Cuts at: 3979 3979
Size: 6409
PstI C_TGCA'G
Cuts at: 830 2332 3761 830
Size: 1502 1429 3478
Fragments arranged by size:
3478 1502 1429
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6409
PvuII CAG'CTG
Cuts at: 2465 3642 2465
Size: 1177 5232
SacI G_AGCT'C
Cuts at: 2202 2202
Size: 6409
SalI G'TCGA_C
Cuts at: 1936 3751 1936
Size: 1815 4594
SanDI GG'GwC_CC
Cuts at: 2813 2813
Size: 6409
SapI GCTCTTCn'nnn_
Cuts at: 2081 2577 2081
Size: 496 5913
SbfI CC_TGCA'GG
Cuts at: 3761 3761
Size: 6409
ScaI AGT'ACT
Cuts at: 4657 6369 4657
Size: 1712 4697
SfcI C'TryA_G
Cuts at: 826 1045 2164 2328 2660 2734 3757 4892 5573
Size: 219 1119 164 332 74 1023 1135 681
Cuts at: 5573 5764 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1119 1023 681 332 219 191
164 74
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6409
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6409
SmaI CCC'GGG
Cuts at: 1872 3251 3736 1872
Size: 1379 485 4545
Fragments arranged by size:
4545 1379 485
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4513 5414 5676 820
Size: 197 926 2570 901 262 1553
Fragments arranged by size:
2570 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4200 493
Size: 3707 2702
SpeI A'CTAG_T
Cuts at: 152 2192 152
Size: 2040 4369
SphI G_CATG'C
Cuts at: 1132 2624 3229 3767 1132
Size: 1492 605 538 3774
Fragments arranged by size:
3774 1492 605 538
SspI AAT'ATT
Cuts at: 5 52 4333 5
Size: 47 4281 2081
Fragments arranged by size:
4281 2081 47
StuI AGG'CCT
Cuts at: 1517 2788 1517
Size: 1271 5138
StyI C'CwwG_G
Cuts at: 513 1065 2004 2233 2253 2833 3660 3848 513
Size: 552 939 229 20 580 827 188 3074
Fragments arranged by size:
3074 939 827 580 552 229 188 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2602 4436 4453 4606 4791 5236 325
Size: 1255 1022 1834 17 153 185 445 1498
Fragments arranged by size:
1834 1498 1255 1022 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2277 2311 4655
Size: 274 80 33 51 1443 300 34 2344
Cuts at: 4655 6367 96
Size: 1712 138
Fragments arranged by size:
2344 1712 1443 300 274 138 80 51
34 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3255 3287 3452 3484 6369 6401 3255
Size: 32 165 32 2885 32 3263
Fragments arranged by size:
3263 2885 165 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3111 1316
Size: 1795 4614
XbaI T'CTAG_A
Cuts at: 3049 3745 3049
Size: 696 5713
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6409
XmaI C'CCGG_G
Cuts at: 1870 3249 3734 1870
Size: 1379 485 4545
Fragments arranged by size:
4545 1379 485
XmnI GAAnn'nnTTC
Cuts at: 2132 2322 4538 2132
Size: 190 2216 4003
Fragments arranged by size:
4003 2216 190
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4217 276
Size: 53 83 186 936 2683 2468
Fragments arranged by size:
2683 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SanDI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI BaeI BbeI BfrBI BplI
BsiWI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI
XcmI