Restriction map for
pFN21AB5977
(Circular) (Six-base) MAPSORT of: pFN21AB5977.seq Check: 6857 from: 1 to: 6262
pFN21AB5977 6262 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4072 278
Size: 53 83 186 936 2536 2468
Fragments arranged by size:
2536 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3605 1937
Size: 1668 4594
Acc65I G'GTAC_C
Cuts at: 3583 3583
Size: 6262
AclI AA'CG_TT
Cuts at: 1563 4389 4762 1563
Size: 2826 373 3063
Fragments arranged by size:
3063 2826 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3315 3564 4325 5709 5977 1632
Size: 1683 249 761 1384 268 1917
Fragments arranged by size:
1917 1683 1384 761 268 249
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6065
AflIII A'CryG_T
Cuts at: 1203 2602 5161 6138 1203
Size: 1399 2559 977 1327
Fragments arranged by size:
2559 1399 1327 977
AgeI A'CCGG_T
Cuts at: 5093 5093
Size: 6262
AhdI GACnn_n'nnGTC
Cuts at: 4991 4991
Size: 6262
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6262
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6262
AlwNI CAG_nnn'CTG
Cuts at: 2830 4005 5577 2830
Size: 1175 1572 3515
Fragments arranged by size:
3515 1572 1175
ApaLI G'TGCA_C
Cuts at: 4319 5475 4319
Size: 1156 5106
ApoI r'AATT_y
Cuts at: 1493 2534 3571 3797 3949 6058 1493
Size: 1041 1037 226 152 2109 1697
Fragments arranged by size:
2109 1697 1041 1037 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4816 160
Size: 872 3784 1606
Fragments arranged by size:
3784 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6262
AvaI C'yCGr_G
Cuts at: 1870 1943 3587 1870
Size: 73 1644 4545
Fragments arranged by size:
4545 1644 73
BamHI G'GATC_C
Cuts at: 2767 3592 3959 2767
Size: 825 367 5070
Fragments arranged by size:
5070 825 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3583 5038 618
Size: 325 217 627 1796 1455 1842
Fragments arranged by size:
1842 1796 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3581 1391
Size: 2190 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2135 2682 928
Size: 971 236 547 4508
Fragments arranged by size:
4508 971 547 236
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3222 3256 4453 4487 1903
Size: 34 1285 34 1197 34 3678
Fragments arranged by size:
3678 1285 1197 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4154 5364 4154
Size: 1210 5052
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6262
BfrBI ATG'CAT
Cuts at: 2915 2915
Size: 6262
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2483 4873
Size: 107 122 71 631 605 337 474 2390
Cuts at: 4873 136
Size: 1525
Fragments arranged by size:
2390 1525 631 605 474 337 122 107
71
BglII A'GATC_T
Cuts at: 2218 6257 2218
Size: 4039 2223
BlpI GC'TnA_GC
Cuts at: 3679 3679
Size: 6262
Bme1580I G_kGCm'C
Cuts at: 1790 4323 5479 1790
Size: 2533 1156 2573
Fragments arranged by size:
2573 2533 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6262
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3535 3993 4951
Size: 416 53 83 399 24 2118 458 958
Cuts at: 4951 442
Size: 1753
Fragments arranged by size:
2118 1753 958 458 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6262
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2040 2733 2040
Size: 693 5569
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2087 2112 2613 4922 1239
Size: 848 25 501 2309 2579
Fragments arranged by size:
2579 2309 848 501 25
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4387 5252 5550 4387
Size: 865 298 5099
Fragments arranged by size:
5099 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4925 882
Size: 617 3426 2219
Fragments arranged by size:
3426 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4053 493
Size: 711 2849 2702
Fragments arranged by size:
2849 2702 711
BsaBI GATnn'nnATC
Cuts at: 6256 6256
Size: 6262
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4069 4451 5086 275
Size: 53 83 186 936 2536 382 635 1451
Fragments arranged by size:
2536 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4695 5093 5367 5514 5925 4695
Size: 398 274 147 411 5032
Fragments arranged by size:
5032 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6037
Fragments arranged by size:
6037 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2016 3131 1186
Size: 830 1115 4317
Fragments arranged by size:
4317 1115 830
BseYI C'CCAG_C
Cuts at: 2032 5465 5961 2032
Size: 3433 496 2333
Fragments arranged by size:
3433 2333 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5689
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2013 3712 4473 5501 1540
Size: 275 186 12 1699 761 1028 2301
Fragments arranged by size:
2301 1699 1028 761 275 186 12
BsiHKAI G_wGCw'C
Cuts at: 4323 4408 5479 4323
Size: 85 1071 5106
Fragments arranged by size:
5106 1071 85
BsmI GAATG_Cn'
Cuts at: 2479 3533 3773 3866 2479
Size: 1054 240 93 4875
Fragments arranged by size:
4875 1054 240 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3581 4323 4408 5479 1391
Size: 399 1791 742 85 1071 2174
Fragments arranged by size:
2174 1791 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5925 5925
Size: 6262
BspHI T'CATG_A
Cuts at: 4151 5881 4151
Size: 1730 4532
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3617 844
Size: 2773 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4149 1394
Size: 429 2326 3507
Fragments arranged by size:
3507 2326 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2328 3555 4757 4931 65
Size: 1183 1080 1227 1202 174 1396
Fragments arranged by size:
1396 1227 1202 1183 1080 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4906 5093 1700
Size: 110 3096 187 2869
Fragments arranged by size:
3096 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6262
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6262
BssSI C'ACGA_G
Cuts at: 4322 5334 4322
Size: 1012 5250
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6262
BstBI TT'CG_AA
Cuts at: 3418 5066 3418
Size: 1648 4614
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6262
BstYI r'GATC_y
Cuts at: 1972 2062 2218 2381 2446 2767 3592 3959 4344
Size: 90 156 163 65 321 825 367 385
Cuts at: 4344 4361 5813 5899 5911 6257 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 825 385 367 346 321 163
156 90 86 65 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4771
Fragments arranged by size:
4771 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5523
Fragments arranged by size:
5523 683 56
BtsI GCAGTG_nn'
Cuts at: 3216 3778 4572 4592 3216
Size: 562 794 20 4886
Fragments arranged by size:
4886 794 562 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6262
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2038 2073 2176 2211 2677 2712 529
Size: 35 1474 35 103 35 466 35 4079
Fragments arranged by size:
4079 1474 466 103 35 35 35 35
DraI TTT'AAA
Cuts at: 3463 3566 3463
Size: 103 6159
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5269 809
Size: 507 3953 1802
Fragments arranged by size:
3953 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 3709 4598
Size: 54 1425 50 275 18 180 1699 889
Cuts at: 4598 6227 8
Size: 1629 43
Fragments arranged by size:
1699 1629 1425 889 275 180 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2010 3709 1537
Size: 275 198 1699 4090
Fragments arranged by size:
4090 1699 275 198
EarI CTCTTCn'nnn_
Cuts at: 1353 2385 4192 1353
Size: 1032 1807 3423
Fragments arranged by size:
3423 1807 1032
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4849 5223 5369 387
Size: 1064 138 3260 374 146 1280
Fragments arranged by size:
3260 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2087 2112 2613 3315 3564 4325 4922
Size: 393 455 25 501 702 249 761 597
Cuts at: 4922 5709 5977 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 761 702 597 501 455 393
268 249 25
EcoO109I rG'GnC_Cy
Cuts at: 2080 2080
Size: 6262
EcoRI G'AATT_C
Cuts at: 3571 3949 3571
Size: 378 5884
EcoRV GAT'ATC
Cuts at: 3405 3405
Size: 6262
FspI TGC'GCA
Cuts at: 4768 4768
Size: 6262
HaeII r_GCGC'y
Cuts at: 1678 5409 1678
Size: 3731 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2669 2701 4903 4935 4977
Size: 32 135 32 1097 32 2202 32 42
Cuts at: 4977 5009 1373
Size: 32 2626
Fragments arranged by size:
2626 2202 1097 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2881 3606 3852 669
Size: 988 281 943 725 246 3079
Fragments arranged by size:
3079 988 943 725 281 246
HindIII A'AGCT_T
Cuts at: 748 3147 748
Size: 2399 3863
HpaI GTT'AAC
Cuts at: 3852 3852
Size: 6262
KpnI G_GTAC'C
Cuts at: 3587 3587
Size: 6262
MfeI C'AATT_G
Cuts at: 3861 3861
Size: 6262
MluI A'CGCG_T
Cuts at: 6138 6138
Size: 6262
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2777 5376 5560 1305
Size: 1472 2599 184 2007
Fragments arranged by size:
2599 2007 1472 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4440
Fragments arranged by size:
4440 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2979 4220 4579 4738 518
Size: 648 1813 1241 359 159 2042
Fragments arranged by size:
2042 1813 1241 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2032 3029 3514 3678 4355 5503 5748 1606
Size: 426 997 485 164 677 1148 245 2120
Fragments arranged by size:
2120 1148 997 677 485 426 245 164
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4771
Fragments arranged by size:
4771 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6262
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6262
NotI GC'GGCC_GC
Cuts at: 3709 3709
Size: 6262
NsiI A_TGCA'T
Cuts at: 2917 2917
Size: 6262
NspI r_CATG'y
Cuts at: 1132 1229 2606 3620 5165 1132
Size: 97 1377 1014 1545 2229
Fragments arranged by size:
2229 1545 1377 1014 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6262
PciI A'CATG_T
Cuts at: 2602 5161 2602
Size: 2559 3703
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6262
PmeI GTTT'AAAC
Cuts at: 3566 3566
Size: 6262
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2069 2101 4331 4363 5844 5876 2069
Size: 32 2230 32 1481 32 2455
Fragments arranged by size:
2455 2230 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2080 2080
Size: 6262
PshAI GACnn'nnGTC
Cuts at: 1613 2657 1613
Size: 1044 5218
PsiI TTA'TAA
Cuts at: 3832 3832
Size: 6262
PstI C_TGCA'G
Cuts at: 830 2152 2302 3211 3614 830
Size: 1322 150 909 403 3478
Fragments arranged by size:
3478 1322 909 403 150
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6262
PvuII CAG'CTG
Cuts at: 3029 3514 3029
Size: 485 5777
SalI G'TCGA_C
Cuts at: 1936 3604 1936
Size: 1668 4594
SbfI CC_TGCA'GG
Cuts at: 3614 3614
Size: 6262
ScaI AGT'ACT
Cuts at: 3482 4510 6222 3482
Size: 1028 1712 3522
Fragments arranged by size:
3522 1712 1028
SfcI C'TryA_G
Cuts at: 826 1045 2148 2298 2762 3207 3610 4745 5426
Size: 219 1103 150 464 445 403 1135 681
Cuts at: 5426 5617 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1103 681 464 445 403 219
191 150
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6262
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6262
SmaI CCC'GGG
Cuts at: 1872 3589 1872
Size: 1717 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4366 5267 5529 820
Size: 197 926 2423 901 262 1553
Fragments arranged by size:
2423 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4053 493
Size: 3560 2702
SpeI A'CTAG_T
Cuts at: 152 3493 152
Size: 3341 2921
SphI G_CATG'C
Cuts at: 1132 3620 1132
Size: 2488 3774
SspI AAT'ATT
Cuts at: 5 52 2597 2847 4186 5
Size: 47 2545 250 1339 2081
Fragments arranged by size:
2545 2081 1339 250 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6262
StyI C'CwwG_G
Cuts at: 513 1065 2004 2655 2991 3701 513
Size: 552 939 651 336 710 3074
Fragments arranged by size:
3074 939 710 651 552 336
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4289 4306 4459 4644 5089 325
Size: 1255 2709 17 153 185 445 1498
Fragments arranged by size:
2709 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3480 4508 6220
Size: 274 80 33 51 1443 1503 1028 1712
Cuts at: 6220 96
Size: 138
Fragments arranged by size:
1712 1503 1443 1028 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6222 6254 6222
Size: 32 6230
Tth111I GACn'n_nGTC
Cuts at: 1316 2876 1316
Size: 1560 4702
XbaI T'CTAG_A
Cuts at: 3086 3598 3086
Size: 512 5750
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2662 2662
Size: 6262
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6262
XmaI C'CCGG_G
Cuts at: 1870 3587 1870
Size: 1717 4545
XmnI GAAnn'nnTTC
Cuts at: 4391 4391
Size: 6262
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4070 276
Size: 53 83 186 936 2536 2468
Fragments arranged by size:
2536 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI