Restriction map for
pFN21AB5975
(Circular) (Six-base) MAPSORT of: pFN21AB5975.seq Check: 8777 from: 1 to: 6262
pFN21AB5975 6262 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2311 2311
Size: 6262
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4072 278
Size: 53 83 186 936 2536 2468
Fragments arranged by size:
2536 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2574 3605 1937
Size: 637 1031 4594
Fragments arranged by size:
4594 1031 637
Acc65I G'GTAC_C
Cuts at: 3583 3583
Size: 6262
AclI AA'CG_TT
Cuts at: 1563 4389 4762 1563
Size: 2826 373 3063
Fragments arranged by size:
3063 2826 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2468 3408 4325 5709 5977 1632
Size: 836 940 917 1384 268 1917
Fragments arranged by size:
1917 1384 940 917 836 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6065
AflIII A'CryG_T
Cuts at: 1203 2279 5161 6138 1203
Size: 1076 2882 977 1327
Fragments arranged by size:
2882 1327 1076 977
AgeI A'CCGG_T
Cuts at: 5093 5093
Size: 6262
AhdI GACnn_n'nnGTC
Cuts at: 4991 4991
Size: 6262
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6262
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2756 2979 1184
Size: 1572 223 4467
Fragments arranged by size:
4467 1572 223
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2023 2055 2023
Size: 32 6230
AlwNI CAG_nnn'CTG
Cuts at: 3388 3431 3458 3512 4005 5577 3388
Size: 43 27 54 493 1572 4073
Fragments arranged by size:
4073 1572 493 54 43 27
ApaLI G'TGCA_C
Cuts at: 4319 5475 4319
Size: 1156 5106
ApoI r'AATT_y
Cuts at: 1493 3239 3571 3797 3949 6058 1493
Size: 1746 332 226 152 2109 1697
Fragments arranged by size:
2109 1746 1697 332 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4816 160
Size: 872 3784 1606
Fragments arranged by size:
3784 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6262
AvaI C'yCGr_G
Cuts at: 1870 1943 3587 1870
Size: 73 1644 4545
Fragments arranged by size:
4545 1644 73
AvrII C'CTAG_G
Cuts at: 2425 2425
Size: 6262
BamHI G'GATC_C
Cuts at: 3592 3959 3592
Size: 367 5895
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2902 3583 5038 618
Size: 325 217 627 1115 681 1455 1842
Fragments arranged by size:
1842 1455 1115 681 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3024 3395 3581 1391
Size: 1633 371 186 4072
Fragments arranged by size:
4072 1633 371 186
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3414 928
Size: 971 1515 3776
Fragments arranged by size:
3776 1515 971
BbvCI CC'TCA_GC
Cuts at: 2010 2010
Size: 6262
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4453 4487 1903
Size: 34 2516 34 3678
Fragments arranged by size:
3678 2516 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4154 5364 4154
Size: 1210 5052
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6262
BfrBI ATG'CAT
Cuts at: 2966 2966
Size: 6262
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2159 2723 4873
Size: 107 122 71 631 605 487 564 2150
Cuts at: 4873 136
Size: 1525
Fragments arranged by size:
2150 1525 631 605 564 487 122 107
71
BglII A'GATC_T
Cuts at: 2330 6257 2330
Size: 3927 2335
BlpI GC'TnA_GC
Cuts at: 3679 3679
Size: 6262
Bme1580I G_kGCm'C
Cuts at: 1790 4323 5479 1790
Size: 2533 1156 2573
Fragments arranged by size:
2573 2533 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6262
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3466 3993 4951
Size: 416 53 83 399 24 2049 527 958
Cuts at: 4951 442
Size: 1753
Fragments arranged by size:
2049 1753 958 527 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6262
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3302 3302
Size: 6262
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2067 2564 3272 3480 3521 4922 1239
Size: 828 497 708 208 41 1401 2579
Fragments arranged by size:
2579 1401 828 708 497 208 41
Bpu10I CC'TnA_GC
Cuts at: 2010 2010
Size: 6262
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2300 3203 4387 5252 5550 2300
Size: 903 1184 865 298 3012
Fragments arranged by size:
3012 1184 903 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4925 882
Size: 617 3426 2219
Fragments arranged by size:
3426 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4053 493
Size: 711 2849 2702
Fragments arranged by size:
2849 2702 711
BsaBI GATnn'nnATC
Cuts at: 6256 6256
Size: 6262
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4069 4451 5086 275
Size: 53 83 186 936 2536 382 635 1451
Fragments arranged by size:
2536 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2136 4695 5093 5367 5514 5925 2136
Size: 2559 398 274 147 411 2473
Fragments arranged by size:
2559 2473 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6037
Fragments arranged by size:
6037 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2027 1186
Size: 841 5421
BseYI C'CCAG_C
Cuts at: 2524 5465 5961 2524
Size: 2941 496 2825
Fragments arranged by size:
2941 2825 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2304 1163
Size: 573 568 5121
Fragments arranged by size:
5121 573 568
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3712 4473 5501 1540
Size: 275 186 1711 761 1028 2301
Fragments arranged by size:
2301 1711 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3024 4323 4408 5479 3024
Size: 1299 85 1071 3807
Fragments arranged by size:
3807 1299 1071 85
BsmI GAATG_Cn'
Cuts at: 3773 3866 3773
Size: 93 6169
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3024 3395 3581 4323 4408 5479 1391
Size: 399 1234 371 186 742 85 1071 2174
Fragments arranged by size:
2174 1234 1071 742 399 371 186 85
BspEI T'CCGG_A
Cuts at: 2136 5925 2136
Size: 3789 2473
BspHI T'CATG_A
Cuts at: 4151 5881 4151
Size: 1730 4532
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2311 3376 3617 844
Size: 1467 1065 241 3489
Fragments arranged by size:
3489 1467 1065 241
BsrBI CCG'CTC
Cuts at: 1394 1823 4149 1394
Size: 429 2326 3507
Fragments arranged by size:
3507 2326 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2689 3423 4757 4931 65
Size: 1183 1441 734 1334 174 1396
Fragments arranged by size:
1441 1396 1334 1183 734 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4906 5093 1700
Size: 110 3096 187 2869
Fragments arranged by size:
3096 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3150 96
Size: 3054 3208
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6262
BssSI C'ACGA_G
Cuts at: 4322 5334 4322
Size: 1012 5250
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6262
BstBI TT'CG_AA
Cuts at: 5066 5066
Size: 6262
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6262
BstXI CCAn_nnnn'nTGG
Cuts at: 2899 2899
Size: 6262
BstYI r'GATC_y
Cuts at: 1972 2330 2532 3328 3592 3959 4344 4361 5813
Size: 358 202 796 264 367 385 17 1452
Cuts at: 5813 5899 5911 6257 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 796 385 367 358 346 264
202 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3451 3451
Size: 6262
BtgI C'CryG_G
Cuts at: 513 1065 2004 2749 513
Size: 552 939 745 4026
Fragments arranged by size:
4026 939 745 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5523
Fragments arranged by size:
5523 683 56
BtsI GCAGTG_nn'
Cuts at: 2592 3045 3778 4572 4592 2592
Size: 453 733 794 20 4262
Fragments arranged by size:
4262 794 733 453 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6262
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6227
DraI TTT'AAA
Cuts at: 3566 3566
Size: 6262
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5269 809
Size: 507 3953 1802
Fragments arranged by size:
3953 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2325 2868 2991
Size: 54 1425 50 275 18 495 543 123
Cuts at: 2991 3709 4598 6227 8
Size: 718 889 1629 43
Fragments arranged by size:
1629 1425 889 718 543 495 275 123
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3709 1537
Size: 275 1897 4090
Fragments arranged by size:
4090 1897 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2276 3498 4192 1353
Size: 923 1222 694 3423
Fragments arranged by size:
3423 1222 923 694
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4849 5223 5369 387
Size: 1064 138 3260 374 146 1280
Fragments arranged by size:
3260 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3022 3022
Size: 6262
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2067 2468 2564 3272 3408 3480 3521
Size: 393 435 401 96 708 136 72 41
Cuts at: 3521 4325 4922 5709 5977 1239
Size: 804 597 787 268 1524
Fragments arranged by size:
1524 804 787 708 597 435 401 393
268 136 96 72 41
EcoNI CCTnn'n_nnAGG
Cuts at: 2318 3218 2318
Size: 900 5362
EcoRI G'AATT_C
Cuts at: 3571 3949 3571
Size: 378 5884
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2195 2554 2737 2195
Size: 359 183 5720
Fragments arranged by size:
5720 359 183
FspI TGC'GCA
Cuts at: 4768 4768
Size: 6262
HaeII r_GCGC'y
Cuts at: 1678 5409 1678
Size: 3731 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2017 2049 3215 3247 3320
Size: 32 135 32 445 32 1166 32 73
Cuts at: 3320 3352 4903 4935 4977 5009 1373
Size: 32 1551 32 42 32 2626
Fragments arranged by size:
2626 1551 1166 445 135 73 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3606 3852 669
Size: 988 281 1668 246 3079
Fragments arranged by size:
3079 1668 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6262
HpaI GTT'AAC
Cuts at: 3852 3852
Size: 6262
KpnI G_GTAC'C
Cuts at: 3587 3587
Size: 6262
MfeI C'AATT_G
Cuts at: 3861 3861
Size: 6262
MluI A'CGCG_T
Cuts at: 6138 6138
Size: 6262
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3554 5376 5560 1305
Size: 2249 1822 184 2007
Fragments arranged by size:
2249 2007 1822 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2327 10
Size: 54 1425 343 495 3945
Fragments arranged by size:
3945 1425 495 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2834 4220 4579 4738 518
Size: 648 1668 1386 359 159 2042
Fragments arranged by size:
2042 1668 1386 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2877 3431 3678 4355 5503 5748 1606
Size: 1271 554 247 677 1148 245 2120
Fragments arranged by size:
2120 1271 1148 677 554 247 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4771
Fragments arranged by size:
4771 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6262
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6262
NotI GC'GGCC_GC
Cuts at: 3709 3709
Size: 6262
NruI TCG'CGA
Cuts at: 2024 2024
Size: 6262
NsiI A_TGCA'T
Cuts at: 2968 2968
Size: 6262
NspI r_CATG'y
Cuts at: 1132 1229 2283 3031 3620 5165 1132
Size: 97 1054 748 589 1545 2229
Fragments arranged by size:
2229 1545 1054 748 589 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6262
PciI A'CATG_T
Cuts at: 2279 5161 2279
Size: 2882 3380
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2542 1108
Size: 1434 4828
PmeI GTTT'AAAC
Cuts at: 3566 3566
Size: 6262
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4331 4363 5844 5876 4331
Size: 32 1481 32 4717
Fragments arranged by size:
4717 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6262
PsiI TTA'TAA
Cuts at: 3832 3832
Size: 6262
PstI C_TGCA'G
Cuts at: 830 3614 830
Size: 2784 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6262
PvuII CAG'CTG
Cuts at: 2877 3431 2877
Size: 554 5708
SacI G_AGCT'C
Cuts at: 3024 3024
Size: 6262
SalI G'TCGA_C
Cuts at: 1936 3604 1936
Size: 1668 4594
SbfI CC_TGCA'GG
Cuts at: 3614 3614
Size: 6262
ScaI AGT'ACT
Cuts at: 4510 6222 4510
Size: 1712 4550
SfcI C'TryA_G
Cuts at: 826 1045 3610 4745 5426 5617 826
Size: 219 2565 1135 681 191 1471
Fragments arranged by size:
2565 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6262
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6262
SmaI CCC'GGG
Cuts at: 1872 3589 1872
Size: 1717 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2315 3218 4366 5267 5529 820
Size: 197 926 372 903 1148 901 262 1553
Fragments arranged by size:
1553 1148 926 903 901 372 262 197
SnaBI TAC'GTA
Cuts at: 493 4053 493
Size: 3560 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6262
SphI G_CATG'C
Cuts at: 1132 3620 1132
Size: 2488 3774
SspI AAT'ATT
Cuts at: 5 52 4186 5
Size: 47 4134 2081
Fragments arranged by size:
4134 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6262
StyI C'CwwG_G
Cuts at: 513 1065 2004 2425 3701 513
Size: 552 939 421 1276 3074
Fragments arranged by size:
3074 1276 939 552 421
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4289 4306 4459 4644 5089 325
Size: 1255 2709 17 153 185 445 1498
Fragments arranged by size:
2709 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3150 4508 6220
Size: 274 80 33 51 1443 1173 1358 1712
Cuts at: 6220 96
Size: 138
Fragments arranged by size:
1712 1443 1358 1173 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6222 6254 6222
Size: 32 6230
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6262
XbaI T'CTAG_A
Cuts at: 3598 3598
Size: 6262
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6262
XmaI C'CCGG_G
Cuts at: 1870 3587 1870
Size: 1717 4545
XmnI GAAnn'nnTTC
Cuts at: 2178 2453 4391 2178
Size: 275 1938 4049
Fragments arranged by size:
4049 1938 275
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4070 276
Size: 53 83 186 936 2536 2468
Fragments arranged by size:
2536 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NruI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI BaeI BbeI BsiWI BsmBI BstZ17I
DraIII EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI