Restriction map for
pFN21AB5972
(Circular) (Six-base) MAPSORT of: pFN21AB5972.seq Check: 2321 from: 1 to: 6388
pFN21AB5972 6388 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4198 278
Size: 53 83 186 936 2662 2468
Fragments arranged by size:
2662 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3731 1937
Size: 1794 4594
Acc65I G'GTAC_C
Cuts at: 3709 3709
Size: 6388
AclI AA'CG_TT
Cuts at: 1563 4515 4888 1563
Size: 2952 373 3063
Fragments arranged by size:
3063 2952 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2364 2995 4451 5835 6103 1632
Size: 732 631 1456 1384 268 1917
Fragments arranged by size:
1917 1456 1384 732 631 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6191
AflIII A'CryG_T
Cuts at: 1203 5287 6264 1203
Size: 4084 977 1327
Fragments arranged by size:
4084 1327 977
AgeI A'CCGG_T
Cuts at: 5219 5219
Size: 6388
AhdI GACnn_n'nnGTC
Cuts at: 5117 5117
Size: 6388
AleI CACnn'nnGTG
Cuts at: 1166 3222 3306 1166
Size: 2056 84 4248
Fragments arranged by size:
4248 2056 84
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6388
AlwNI CAG_nnn'CTG
Cuts at: 2077 2385 4131 5703 2077
Size: 308 1746 1572 2762
Fragments arranged by size:
2762 1746 1572 308
ApaLI G'TGCA_C
Cuts at: 4445 5601 4445
Size: 1156 5232
ApoI r'AATT_y
Cuts at: 1493 2219 2476 2935 2961 3341 3697 3923 4075
Size: 726 257 459 26 380 356 226 152
Cuts at: 4075 6184 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 726 459 380 356 257 226
152 26
AseI AT'TA_AT
Cuts at: 160 1032 2246 2250 4942 160
Size: 872 1214 4 2692 1606
Fragments arranged by size:
2692 1606 1214 872 4
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6388
AvaI C'yCGr_G
Cuts at: 1870 1943 3713 1870
Size: 73 1770 4545
Fragments arranged by size:
4545 1770 73
BamHI G'GATC_C
Cuts at: 3718 4085 3718
Size: 367 6021
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3709 5164 618
Size: 325 217 627 1922 1455 1842
Fragments arranged by size:
1922 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3707 1391
Size: 2316 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2403 2927 3388 928
Size: 971 504 524 461 3928
Fragments arranged by size:
3928 971 524 504 461
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4579 4613 1903
Size: 34 2642 34 3678
Fragments arranged by size:
3678 2642 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4280 5490 4280
Size: 1210 5178
BclI T'GATC_A
Cuts at: 1548 2007 1548
Size: 459 5929
BfrBI ATG'CAT
Cuts at: 3164 3164
Size: 6388
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4999 136
Size: 107 122 71 631 605 3327 1525
Fragments arranged by size:
3327 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6383 6383
Size: 6388
BlpI GC'TnA_GC
Cuts at: 3805 3805
Size: 6388
Bme1580I G_kGCm'C
Cuts at: 1790 4449 5605 1790
Size: 2659 1156 2573
Fragments arranged by size:
2659 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6388
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4119 5077 442
Size: 416 53 83 399 24 2702 958 1753
Fragments arranged by size:
2702 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6388
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2138 5048 1239
Size: 899 2910 2579
Fragments arranged by size:
2910 2579 899
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2043 2162 4513 5378 5676 2043
Size: 119 2351 865 298 2755
Fragments arranged by size:
2755 2351 865 298 119
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2971 5051 882
Size: 617 1472 2080 2219
Fragments arranged by size:
2219 2080 1472 617
BsaAI yAC'GTr
Cuts at: 493 1204 4179 493
Size: 711 2975 2702
Fragments arranged by size:
2975 2702 711
BsaBI GATnn'nnATC
Cuts at: 6382 6382
Size: 6388
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4195 4577 5212 275
Size: 53 83 186 936 2662 382 635 1451
Fragments arranged by size:
2662 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3359 4821 5219 5493 5640 6051 3359
Size: 1462 398 274 147 411 3696
Fragments arranged by size:
3696 1462 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6163
Fragments arranged by size:
6163 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2078 2210 1186
Size: 892 132 5364
Fragments arranged by size:
5364 892 132
BseYI C'CCAG_C
Cuts at: 5591 6087 5591
Size: 496 5892
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5815
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3838 4599 5627 1540
Size: 275 186 1837 761 1028 2301
Fragments arranged by size:
2301 1837 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4449 4534 5605 4449
Size: 85 1071 5232
Fragments arranged by size:
5232 1071 85
BsmI GAATG_Cn'
Cuts at: 2154 3161 3899 3992 2154
Size: 1007 738 93 4550
Fragments arranged by size:
4550 1007 738 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3707 4449 4534 5605 1391
Size: 399 1917 742 85 1071 2174
Fragments arranged by size:
2174 1917 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6051 6051
Size: 6388
BspHI T'CATG_A
Cuts at: 2824 2908 2992 3076 4277 6007 2824
Size: 84 84 84 1201 1730 3205
Fragments arranged by size:
3205 1730 1201 84 84 84
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2531 3743 844
Size: 1687 1212 3489
Fragments arranged by size:
3489 1687 1212
BsrBI CCG'CTC
Cuts at: 1394 1823 4275 1394
Size: 429 2452 3507
Fragments arranged by size:
3507 2452 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3534 4883 5057 65
Size: 1183 2286 1349 174 1396
Fragments arranged by size:
2286 1396 1349 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5032 5219 1700
Size: 110 3222 187 2869
Fragments arranged by size:
3222 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2100 2491 3622 96
Size: 2004 391 1131 2862
Fragments arranged by size:
2862 2004 1131 391
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6388
BssSI C'ACGA_G
Cuts at: 4448 5460 4448
Size: 1012 5376
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6388
BstBI TT'CG_AA
Cuts at: 5192 5192
Size: 6388
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6388
BstYI r'GATC_y
Cuts at: 1972 3718 4085 4470 4487 5939 6025 6037 6383
Size: 1746 367 385 17 1452 86 12 346
Cuts at: 6383 1972
Size: 1977
Fragments arranged by size:
1977 1746 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2201 513
Size: 552 1136 4700
Fragments arranged by size:
4700 1136 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5649
Fragments arranged by size:
5649 683 56
BtsI GCAGTG_nn'
Cuts at: 3904 4698 4718 3904
Size: 794 20 5574
Fragments arranged by size:
5574 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6388
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2048 2083 2437 2472 529
Size: 35 1484 35 354 35 4445
Fragments arranged by size:
4445 1484 354 35 35 35
DraI TTT'AAA
Cuts at: 3692 3692
Size: 6388
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5395 809
Size: 507 4079 1802
Fragments arranged by size:
4079 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3835 4724 6353
Size: 54 1425 50 275 18 2005 889 1629
Cuts at: 6353 8
Size: 43
Fragments arranged by size:
2005 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3835 1537
Size: 275 2023 4090
Fragments arranged by size:
4090 2023 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2340 2489 4318 1353
Size: 987 149 1829 3423
Fragments arranged by size:
3423 1829 987 149
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4975 5349 5495 387
Size: 1064 138 3386 374 146 1280
Fragments arranged by size:
3386 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2138 2364 2995 4451 5048 5835 6103
Size: 393 506 226 631 1456 597 787 268
Cuts at: 6103 1239
Size: 1524
Fragments arranged by size:
1524 1456 787 631 597 506 393 268
226
EcoO109I rG'GnC_Cy
Cuts at: 2094 2094
Size: 6388
EcoRI G'AATT_C
Cuts at: 2476 2935 2961 3697 4075 2476
Size: 459 26 736 378 4789
Fragments arranged by size:
4789 736 459 378 26
FspI TGC'GCA
Cuts at: 4894 4894
Size: 6388
HaeII r_GCGC'y
Cuts at: 1678 5535 1678
Size: 3857 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2613 2645 5029 5061 5103
Size: 32 135 32 1041 32 2384 32 42
Cuts at: 5103 5135 1373
Size: 32 2626
Fragments arranged by size:
2626 2384 1041 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3024 3276 3732 3978 669
Size: 988 281 1086 252 456 246 3079
Fragments arranged by size:
3079 1086 988 456 281 252 246
HindIII A'AGCT_T
Cuts at: 748 2463 748
Size: 1715 4673
HpaI GTT'AAC
Cuts at: 3978 3978
Size: 6388
KpnI G_GTAC'C
Cuts at: 3713 3713
Size: 6388
MfeI C'AATT_G
Cuts at: 3987 3987
Size: 6388
MluI A'CGCG_T
Cuts at: 6264 6264
Size: 6388
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2163 5502 5686 1305
Size: 858 3339 184 2007
Fragments arranged by size:
3339 2007 858 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4566
Fragments arranged by size:
4566 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2661 2829 2913 2997 3070 3081 3222
Size: 648 1495 168 84 84 73 11 141
Cuts at: 3222 3306 4346 4705 4864 518
Size: 84 1040 359 159 2042
Fragments arranged by size:
2042 1495 1040 648 359 168 159 141
84 84 84 73 11
MspA1I CmG'CkG
Cuts at: 1606 3804 4481 5629 5874 1606
Size: 2198 677 1148 245 2120
Fragments arranged by size:
2198 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2201 513
Size: 552 1136 4700
Fragments arranged by size:
4700 1136 552
NdeI CA'TA_TG
Cuts at: 387 3067 387
Size: 2680 3708
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6388
NotI GC'GGCC_GC
Cuts at: 3835 3835
Size: 6388
NsiI A_TGCA'T
Cuts at: 3166 3166
Size: 6388
NspI r_CATG'y
Cuts at: 1132 1229 3168 3746 5291 1132
Size: 97 1939 578 1545 2229
Fragments arranged by size:
2229 1939 1545 578 97
PacI TTA_AT'TAA
Cuts at: 2250 2250
Size: 6388
PasI CC'CwG_GG
Cuts at: 1348 2017 1348
Size: 669 5719
PciI A'CATG_T
Cuts at: 5287 5287
Size: 6388
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6388
PmeI GTTT'AAAC
Cuts at: 3692 3692
Size: 6388
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4457 4489 5970 6002 4457
Size: 32 1481 32 4843
Fragments arranged by size:
4843 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2094 2094
Size: 6388
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6388
PsiI TTA'TAA
Cuts at: 3958 3958
Size: 6388
PstI C_TGCA'G
Cuts at: 830 3740 830
Size: 2910 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6388
SalI G'TCGA_C
Cuts at: 1936 3730 1936
Size: 1794 4594
SbfI CC_TGCA'GG
Cuts at: 3740 3740
Size: 6388
ScaI AGT'ACT
Cuts at: 4636 6348 4636
Size: 1712 4676
SfcI C'TryA_G
Cuts at: 826 1045 2125 3736 4871 5552 5743 826
Size: 219 1080 1611 1135 681 191 1471
Fragments arranged by size:
1611 1471 1135 1080 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6388
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6388
SmaI CCC'GGG
Cuts at: 1872 3715 1872
Size: 1843 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2058 2177 4492 5393 5655 820
Size: 197 926 115 119 2315 901 262 1553
Fragments arranged by size:
2315 1553 926 901 262 197 119 115
SnaBI TAC'GTA
Cuts at: 493 4179 493
Size: 3686 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6388
SphI G_CATG'C
Cuts at: 1132 3746 1132
Size: 2614 3774
SspI AAT'ATT
Cuts at: 5 52 2728 4312 5
Size: 47 2676 1584 2081
Fragments arranged by size:
2676 2081 1584 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6388
StyI C'CwwG_G
Cuts at: 513 1065 2201 3827 513
Size: 552 1136 1626 3074
Fragments arranged by size:
3074 1626 1136 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2861 4415 4432 4585 4770 5215 325
Size: 1255 1281 1554 17 153 185 445 1498
Fragments arranged by size:
1554 1498 1281 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2100 2491 3622
Size: 274 80 33 51 1443 123 391 1131
Cuts at: 3622 4634 6346 96
Size: 1012 1712 138
Fragments arranged by size:
1712 1443 1131 1012 391 274 138 123
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2536 2568 6348 6380 2536
Size: 32 3780 32 2544
Fragments arranged by size:
3780 2544 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6388
XbaI T'CTAG_A
Cuts at: 3724 3724
Size: 6388
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6388
XmaI C'CCGG_G
Cuts at: 1870 3713 1870
Size: 1843 4545
XmnI GAAnn'nnTTC
Cuts at: 2172 2220 2266 2334 2381 2465 2643 2924 3008
Size: 48 46 68 47 84 178 281 84
Cuts at: 3008 3092 3260 3327 3428 4517 2172
Size: 84 168 67 101 1089 4043
Fragments arranged by size:
4043 1089 281 178 168 101 84 84
84 68 67 48 47 46
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4196 276
Size: 53 83 186 936 2662 2468
Fragments arranged by size:
2662 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PacI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI PfoI PmlI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI