Restriction map for
pFN21AB5971
(Circular) (Six-base) MAPSORT of: pFN21AB5971.seq Check: 5711 from: 1 to: 6259
pFN21AB5971 6259 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2789 2789
Size: 6259
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4069 278
Size: 53 83 186 936 2533 2468
Fragments arranged by size:
2533 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3068 3602 1937
Size: 1131 534 4594
Fragments arranged by size:
4594 1131 534
Acc65I G'GTAC_C
Cuts at: 3580 3580
Size: 6259
AclI AA'CG_TT
Cuts at: 1563 4386 4759 1563
Size: 2823 373 3063
Fragments arranged by size:
3063 2823 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2203 2310 3203 3315 4322 5706 5974 1632
Size: 571 107 893 112 1007 1384 268 1917
Fragments arranged by size:
1917 1384 1007 893 571 268 112 107
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6062
AflIII A'CryG_T
Cuts at: 1203 2466 3483 5158 6135 1203
Size: 1263 1017 1675 977 1327
Fragments arranged by size:
1675 1327 1263 1017 977
AgeI A'CCGG_T
Cuts at: 5090 5090
Size: 6259
AhdI GACnn_n'nnGTC
Cuts at: 4988 4988
Size: 6259
AleI CACnn'nnGTG
Cuts at: 1166 2974 1166
Size: 1808 4451
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2980 1184
Size: 1796 4463
AlwNI CAG_nnn'CTG
Cuts at: 2082 2285 2798 4002 5574 2082
Size: 203 513 1204 1572 2767
Fragments arranged by size:
2767 1572 1204 513 203
ApaI G_GGCC'C
Cuts at: 2404 2730 3393 2404
Size: 326 663 5270
Fragments arranged by size:
5270 663 326
ApaLI G'TGCA_C
Cuts at: 2977 4316 5472 2977
Size: 1339 1156 3764
Fragments arranged by size:
3764 1339 1156
ApoI r'AATT_y
Cuts at: 1493 3568 3794 3946 6055 1493
Size: 2075 226 152 2109 1697
Fragments arranged by size:
2109 2075 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4813 160
Size: 872 3781 1606
Fragments arranged by size:
3781 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6259
AvaI C'yCGr_G
Cuts at: 1870 1943 3093 3498 3584 1870
Size: 73 1150 405 86 4545
Fragments arranged by size:
4545 1150 405 86 73
BamHI G'GATC_C
Cuts at: 3589 3956 3589
Size: 367 5892
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2703 2801 3005 3580 5035
Size: 325 217 627 916 98 204 575 1455
Cuts at: 5035 618
Size: 1842
Fragments arranged by size:
1842 1455 916 627 575 325 217 204
98
BanII G_rGCy'C
Cuts at: 1391 2181 2383 2404 2730 2909 3393 3578 1391
Size: 790 202 21 326 179 484 185 4072
Fragments arranged by size:
4072 790 484 326 202 185 179 21
BbeI G_GCGC'C
Cuts at: 2805 2805
Size: 6259
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2453 3284 928
Size: 971 554 831 3903
Fragments arranged by size:
3903 971 831 554
BbvCI CC'TCA_GC
Cuts at: 3141 3141
Size: 6259
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2105 2139 4450 4484 1903
Size: 34 168 34 2311 34 3678
Fragments arranged by size:
3678 2311 168 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4151 5361 4151
Size: 1210 5049
BclI T'GATC_A
Cuts at: 1548 2580 1548
Size: 1032 5227
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2093 2304 2503
Size: 107 122 71 631 605 421 211 199
Cuts at: 2503 2561 4870 136
Size: 58 2309 1525
Fragments arranged by size:
2309 1525 631 605 421 211 199 122
107 71 58
BglII A'GATC_T
Cuts at: 6254 6254
Size: 6259
BlpI GC'TnA_GC
Cuts at: 3676 3676
Size: 6259
Bme1580I G_kGCm'C
Cuts at: 1790 2404 2730 2969 2981 3393 4320 5476 1790
Size: 614 326 239 12 412 927 1156 2573
Fragments arranged by size:
2573 1156 927 614 412 326 239 12
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6259
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2273 2635 3141
Size: 416 53 83 399 24 856 362 506
Cuts at: 3141 3990 4948 442
Size: 849 958 1753
Fragments arranged by size:
1753 958 856 849 506 416 399 362
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6259
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2126 3267 3565 4919 1239
Size: 887 1141 298 1354 2579
Fragments arranged by size:
2579 1354 1141 887 298
Bpu10I CC'TnA_GC
Cuts at: 2499 3141 2499
Size: 642 5617
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2956 2999 4384 5249 5547 2956
Size: 43 1385 865 298 3668
Fragments arranged by size:
3668 1385 865 298 43
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2038 4922 882
Size: 617 539 2884 2219
Fragments arranged by size:
2884 2219 617 539
BsaAI yAC'GTr
Cuts at: 493 1204 4050 493
Size: 711 2846 2702
Fragments arranged by size:
2846 2702 711
BsaBI GATnn'nnATC
Cuts at: 6253 6253
Size: 6259
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2802 4066 4448 5083
Size: 53 83 186 936 1269 1264 382 635
Cuts at: 5083 275
Size: 1451
Fragments arranged by size:
1451 1269 1264 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4692 5090 5364 5511 5922 4692
Size: 398 274 147 411 5029
Fragments arranged by size:
5029 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2807 2837 3506 3536 1178
Size: 30 165 30 1404 30 669 30 3901
Fragments arranged by size:
3901 1404 669 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2396 3511 1186
Size: 1210 1115 3934
Fragments arranged by size:
3934 1210 1115
BseYI C'CCAG_C
Cuts at: 2075 2842 3009 3554 5462 5958 2075
Size: 767 167 545 1908 496 2376
Fragments arranged by size:
2376 1908 767 545 496 167
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2949 1163
Size: 573 1213 4473
Fragments arranged by size:
4473 1213 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3709 4470 5498 1540
Size: 275 186 1708 761 1028 2301
Fragments arranged by size:
2301 1708 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2981 4320 4405 5476 2981
Size: 1339 85 1071 3764
Fragments arranged by size:
3764 1339 1071 85
BsmI GAATG_Cn'
Cuts at: 2651 3770 3863 2651
Size: 1119 93 5047
Fragments arranged by size:
5047 1119 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2181 2383 2404 2730 2909 2969 2981
Size: 399 391 202 21 326 179 60 12
Cuts at: 2981 3393 3578 4320 4405 5476 1391
Size: 412 185 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 412 399 391 326 202
185 179 85 60 21 12
BspEI T'CCGG_A
Cuts at: 5922 5922
Size: 6259
BspHI T'CATG_A
Cuts at: 3267 4148 5878 3267
Size: 881 1730 3648
Fragments arranged by size:
3648 1730 881
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2789 3614 844
Size: 1945 825 3489
Fragments arranged by size:
3489 1945 825
BsrBI CCG'CTC
Cuts at: 1394 1823 2503 4146 1394
Size: 429 680 1643 3507
Fragments arranged by size:
3507 1643 680 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4754 4928 65
Size: 1183 3506 174 1396
Fragments arranged by size:
3506 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4903 5090 1700
Size: 110 3093 187 2869
Fragments arranged by size:
3093 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6259
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6259
BssSI C'ACGA_G
Cuts at: 4319 5331 4319
Size: 1012 5247
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6259
BstBI TT'CG_AA
Cuts at: 5063 5063
Size: 6259
BstEII G'GTnAC_C
Cuts at: 1184 2591 1184
Size: 1407 4852
BstYI r'GATC_y
Cuts at: 1972 2421 3018 3589 3956 4341 4358 5810 5896
Size: 449 597 571 367 385 17 1452 86
Cuts at: 5896 5908 6254 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 597 571 449 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2895 2895
Size: 6259
BtgI C'CryG_G
Cuts at: 513 1065 2091 2097 513
Size: 552 1026 6 4675
Fragments arranged by size:
4675 1026 552 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5520
Fragments arranged by size:
5520 683 56
BtsI GCAGTG_nn'
Cuts at: 2914 3303 3775 4569 4589 2914
Size: 389 472 794 20 4584
Fragments arranged by size:
4584 794 472 389 20
ClaI AT'CG_AT
Cuts at: 1554 2736 1554
Size: 1182 5077
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6224
DraI TTT'AAA
Cuts at: 3563 3563
Size: 6259
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5266 809
Size: 507 3950 1802
Fragments arranged by size:
3950 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2094 3300 3706
Size: 54 1425 50 275 18 264 1206 406
Cuts at: 3706 4595 6224 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1206 889 406 275 264 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3706 1537
Size: 275 1894 4090
Fragments arranged by size:
4090 1894 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2649 3179 3282 4189 1353
Size: 1296 530 103 907 3423
Fragments arranged by size:
3423 1296 907 530 103
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4846 5220 5366 387
Size: 1064 138 3257 374 146 1280
Fragments arranged by size:
3257 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2126 2203 2310 3203 3267 3315 3565
Size: 393 494 77 107 893 64 48 250
Cuts at: 3565 4322 4919 5706 5974 1239
Size: 757 597 787 268 1524
Fragments arranged by size:
1524 893 787 757 597 494 393 268
250 107 77 64 48
EcoO109I rG'GnC_Cy
Cuts at: 2400 2401 2659 2726 2727 2772 2853 3389 3390
Size: 1 258 67 1 45 81 536 1
Cuts at: 3390 3458 3495 3503 2400
Size: 68 37 8 5156
Fragments arranged by size:
5156 536 258 81 68 67 45 37
8 1 1 1
EcoRI G'AATT_C
Cuts at: 3568 3946 3568
Size: 378 5881
EcoRV GAT'ATC
Cuts at: 2131 2131
Size: 6259
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2539 3208 2539
Size: 669 5590
FspI TGC'GCA
Cuts at: 3398 4765 3398
Size: 1367 4892
HaeII r_GCGC'y
Cuts at: 1678 2805 3264 5406 1678
Size: 1127 459 2142 2531
Fragments arranged by size:
2531 2142 1127 459
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3506 3538 4900 4932 4974
Size: 32 135 32 1934 32 1362 32 42
Cuts at: 4974 5006 1373
Size: 32 2626
Fragments arranged by size:
2626 1934 1362 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2638 3069 3603 3849 669
Size: 988 281 700 431 534 246 3079
Fragments arranged by size:
3079 988 700 534 431 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6259
HpaI GTT'AAC
Cuts at: 3849 3849
Size: 6259
KasI G'GCGC_C
Cuts at: 2801 2801
Size: 6259
KpnI G_GTAC'C
Cuts at: 3584 3584
Size: 6259
MfeI C'AATT_G
Cuts at: 3858 3858
Size: 6259
MluI A'CGCG_T
Cuts at: 6135 6135
Size: 6259
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2779 3039 5373 5557 1305
Size: 1474 260 2334 184 2007
Fragments arranged by size:
2334 2007 1474 260 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3302 10
Size: 54 1425 343 1470 2967
Fragments arranged by size:
2967 1470 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2628 2974 4217 4576 4735 518
Size: 648 1462 346 1243 359 159 2042
Fragments arranged by size:
2042 1462 1243 648 359 346 159
MspA1I CmG'CkG
Cuts at: 1606 2748 3675 4352 5500 5745 1606
Size: 1142 927 677 1148 245 2120
Fragments arranged by size:
2120 1148 1142 927 677 245
NarI GG'CG_CC
Cuts at: 2802 2802
Size: 6259
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5707
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6259
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6259
NotI GC'GGCC_GC
Cuts at: 3706 3706
Size: 6259
NspI r_CATG'y
Cuts at: 1132 1229 3108 3487 3617 5162 1132
Size: 97 1879 379 130 1545 2229
Fragments arranged by size:
2229 1879 1545 379 130 97
PasI CC'CwG_GG
Cuts at: 1348 2489 2663 1348
Size: 1141 174 4944
Fragments arranged by size:
4944 1141 174
PciI A'CATG_T
Cuts at: 3483 5158 3483
Size: 1675 4584
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6259
PfoI T'CCnGG_A
Cuts at: 2142 2142
Size: 6259
PmeI GTTT'AAAC
Cuts at: 3563 3563
Size: 6259
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4328 4360 5841 5873 4328
Size: 32 1481 32 4714
Fragments arranged by size:
4714 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2659 2853 3458 3503 2659
Size: 194 605 45 5415
Fragments arranged by size:
5415 605 194 45
PshAI GACnn'nnGTC
Cuts at: 1613 2858 1613
Size: 1245 5014
PsiI TTA'TAA
Cuts at: 2202 3829 2202
Size: 1627 4632
PspOMI G'GGCC_C
Cuts at: 2400 2726 3389 2400
Size: 326 663 5270
Fragments arranged by size:
5270 663 326
PstI C_TGCA'G
Cuts at: 830 3078 3611 830
Size: 2248 533 3478
Fragments arranged by size:
3478 2248 533
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6259
SalI G'TCGA_C
Cuts at: 1936 3067 3601 1936
Size: 1131 534 4594
Fragments arranged by size:
4594 1131 534
SapI GCTCTTCn'nnn_
Cuts at: 3179 3179
Size: 6259
SbfI CC_TGCA'GG
Cuts at: 3611 3611
Size: 6259
ScaI AGT'ACT
Cuts at: 4507 6219 4507
Size: 1712 4547
SfcI C'TryA_G
Cuts at: 826 1045 3074 3607 4742 5423 5614 826
Size: 219 2029 533 1135 681 191 1471
Fragments arranged by size:
2029 1471 1135 681 533 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2304 2304
Size: 6259
SfoI GGC'GCC
Cuts at: 2803 2803
Size: 6259
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6259
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6259
SmaI CCC'GGG
Cuts at: 1872 3095 3500 3586 1872
Size: 1223 405 86 4545
Fragments arranged by size:
4545 1223 405 86
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2935 3014 4363 5264 5526 820
Size: 197 926 992 79 1349 901 262 1553
Fragments arranged by size:
1553 1349 992 926 901 262 197 79
SnaBI TAC'GTA
Cuts at: 493 4050 493
Size: 3557 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6259
SphI G_CATG'C
Cuts at: 1132 3617 1132
Size: 2485 3774
SspI AAT'ATT
Cuts at: 5 52 4183 5
Size: 47 4131 2081
Fragments arranged by size:
4131 2081 47
StuI AGG'CCT
Cuts at: 1517 2298 2497 2879 3139 1517
Size: 781 199 382 260 4637
Fragments arranged by size:
4637 781 382 260 199
StyI C'CwwG_G
Cuts at: 513 1065 3698 513
Size: 552 2633 3074
Fragments arranged by size:
3074 2633 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2333 2579 2915 3023 4286 4303 4456
Size: 1255 753 246 336 108 1263 17 153
Cuts at: 4456 4641 5086 325
Size: 185 445 1498
Fragments arranged by size:
1498 1263 1255 753 445 336 246 185
153 108 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2469 4505 6217
Size: 274 80 33 51 1443 492 2036 1712
Cuts at: 6217 96
Size: 138
Fragments arranged by size:
2036 1712 1443 492 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6219 6251 6219
Size: 32 6227
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6259
XbaI T'CTAG_A
Cuts at: 3595 3595
Size: 6259
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2486 2670 2816 2011
Size: 475 184 146 5454
Fragments arranged by size:
5454 475 184 146
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6259
XmaI C'CCGG_G
Cuts at: 1870 3093 3498 3584 1870
Size: 1223 405 86 4545
Fragments arranged by size:
4545 1223 405 86
XmnI GAAnn'nnTTC
Cuts at: 4388 4388
Size: 6259
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4067 276
Size: 53 83 186 936 2533 2468
Fragments arranged by size:
2533 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SalI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
PvuII RsrII SacI SacII SanDI SexAI SrfI SwaI