Restriction map for
pFN21AB5970
(Circular) (Six-base) MAPSORT of: pFN21AB5970.seq Check: 3070 from: 1 to: 6385
pFN21AB5970 6385 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4195 278
Size: 53 83 186 936 2659 2468
Fragments arranged by size:
2659 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3411 3728 1937
Size: 1474 317 4594
Fragments arranged by size:
4594 1474 317
Acc65I G'GTAC_C
Cuts at: 3706 3706
Size: 6385
AclI AA'CG_TT
Cuts at: 1563 4512 4885 1563
Size: 2949 373 3063
Fragments arranged by size:
3063 2949 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3308 3557 4448 5832 6100 1632
Size: 1676 249 891 1384 268 1917
Fragments arranged by size:
1917 1676 1384 891 268 249
AfeI AGC'GCT
Cuts at: 3276 3428 3276
Size: 152 6233
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6188
AflIII A'CryG_T
Cuts at: 1203 5284 6261 1203
Size: 4081 977 1327
Fragments arranged by size:
4081 1327 977
AgeI A'CCGG_T
Cuts at: 2318 5216 2318
Size: 2898 3487
AhdI GACnn_n'nnGTC
Cuts at: 3103 5114 3103
Size: 2011 4374
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6385
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6385
AlwNI CAG_nnn'CTG
Cuts at: 2392 2701 3304 4128 5700 2392
Size: 309 603 824 1572 3077
Fragments arranged by size:
3077 1572 824 603 309
ApaI G_GGCC'C
Cuts at: 2275 2285 2275
Size: 10 6375
ApaLI G'TGCA_C
Cuts at: 4442 5598 4442
Size: 1156 5229
ApoI r'AATT_y
Cuts at: 1493 3694 3920 4072 6181 1493
Size: 2201 226 152 2109 1697
Fragments arranged by size:
2201 2109 1697 226 152
AscI GG'CGCG_CC
Cuts at: 3131 3218 3566 3131
Size: 87 348 5950
Fragments arranged by size:
5950 348 87
AseI AT'TA_AT
Cuts at: 160 1032 4939 160
Size: 872 3907 1606
Fragments arranged by size:
3907 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6385
AvaI C'yCGr_G
Cuts at: 1870 1943 2286 2574 2843 2922 3184 3443 3710
Size: 73 343 288 269 79 262 259 267
Cuts at: 3710 1870
Size: 4545
Fragments arranged by size:
4545 343 288 269 267 262 259 79
73
BamHI G'GATC_C
Cuts at: 2950 3079 3328 3715 4082 2950
Size: 129 249 387 367 5253
Fragments arranged by size:
5253 387 367 249 129
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2102 2552 3109 3706 5161
Size: 325 217 627 315 450 557 597 1455
Cuts at: 5161 618
Size: 1842
Fragments arranged by size:
1842 1455 627 597 557 450 325 315
217
BanII G_rGCy'C
Cuts at: 1391 2059 2230 2275 2285 2457 2775 3405 3704
Size: 668 171 45 10 172 318 630 299
Cuts at: 3704 1391
Size: 4072
Fragments arranged by size:
4072 668 630 318 299 172 171 45
10
BbeI G_GCGC'C
Cuts at: 3113 3113
Size: 6385
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2133 928
Size: 971 234 5180
Fragments arranged by size:
5180 971 234
BbvCI CC'TCA_GC
Cuts at: 2441 3053 2441
Size: 612 5773
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4576 4610 1903
Size: 34 50 34 2555 34 3678
Fragments arranged by size:
3678 2555 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4277 5487 4277
Size: 1210 5175
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6385
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2054 2827 3243
Size: 107 122 71 631 605 382 773 416
Cuts at: 3243 3252 3554 3563 4996 136
Size: 9 302 9 1433 1525
Fragments arranged by size:
1525 1433 773 631 605 416 382 302
122 107 71 9 9
BglII A'GATC_T
Cuts at: 6380 6380
Size: 6385
BlpI GC'TnA_GC
Cuts at: 3404 3802 3404
Size: 398 5987
Bme1580I G_kGCm'C
Cuts at: 1790 2105 2275 2285 2557 2814 3352 3588 3654
Size: 315 170 10 272 257 538 236 66
Cuts at: 3654 4446 5602 1790
Size: 792 1156 2573
Fragments arranged by size:
2573 1156 792 538 315 272 257 236
170 66 10
BmgBI CAC'GTC
Cuts at: 1318 3105 1318
Size: 1787 4598
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2189 4116 5074
Size: 416 53 83 399 24 772 1927 958
Cuts at: 5074 442
Size: 1753
Fragments arranged by size:
1927 1753 958 772 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6385
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2127 2429 2484 2897 3032 5045 1239
Size: 888 302 55 413 135 2013 2579
Fragments arranged by size:
2579 2013 888 413 302 135 55
Bpu10I CC'TnA_GC
Cuts at: 2441 3053 2441
Size: 612 5773
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4510 5375 5673 4510
Size: 865 298 5222
Fragments arranged by size:
5222 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2283 3440 5048 882
Size: 617 784 1157 1608 2219
Fragments arranged by size:
2219 1608 1157 784 617
BsaAI yAC'GTr
Cuts at: 493 1204 3523 3649 4176 493
Size: 711 2319 126 527 2702
Fragments arranged by size:
2702 2319 711 527 126
BsaBI GATnn'nnATC
Cuts at: 6379 6379
Size: 6385
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3110 4192 4574 5209
Size: 53 83 186 936 1577 1082 382 635
Cuts at: 5209 275
Size: 1451
Fragments arranged by size:
1577 1451 1082 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2318 2953 4818 5216 5490 5637 6048 2318
Size: 635 1865 398 274 147 411 2655
Fragments arranged by size:
2655 1865 635 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6160
Fragments arranged by size:
6160 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2102 2481 2861 3047 3641 1186
Size: 916 379 380 186 594 3930
Fragments arranged by size:
3930 916 594 380 379 186
BseYI C'CCAG_C
Cuts at: 2062 2277 2356 2540 2858 5588 6084 2062
Size: 215 79 184 318 2730 496 2363
Fragments arranged by size:
2730 2363 496 318 215 184 79
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2285 3077 1163
Size: 573 549 792 4471
Fragments arranged by size:
4471 792 573 549
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3499 3835 4596 5624 1540
Size: 275 186 1498 336 761 1028 2301
Fragments arranged by size:
2301 1498 1028 761 336 275 186
BsiHKAI G_wGCw'C
Cuts at: 3405 4446 4531 5602 3405
Size: 1041 85 1071 4188
Fragments arranged by size:
4188 1071 1041 85
BsmI GAATG_Cn'
Cuts at: 3896 3989 3896
Size: 93 6292
BsmBI CGTCTCn'nnnn_
Cuts at: 2873 2873
Size: 6385
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2059 2105 2230 2275 2285 2457 2557
Size: 399 269 46 125 45 10 172 100
Cuts at: 2557 2775 2814 3352 3405 3588 3654 3704 4446
Size: 218 39 538 53 183 66 50 742
Cuts at: 4446 4531 5602 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 538 399 269 218 183
172 125 100 85 66 53 50 46
45 39 10
BspEI T'CCGG_A
Cuts at: 2953 6048 2953
Size: 3095 3290
BspHI T'CATG_A
Cuts at: 4274 6004 4274
Size: 1730 4655
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2022 3740 844
Size: 1178 1718 3489
Fragments arranged by size:
3489 1718 1178
BsrBI CCG'CTC
Cuts at: 1394 1823 4272 1394
Size: 429 2449 3507
Fragments arranged by size:
3507 2449 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2200 4880 5054 65
Size: 1183 952 2680 174 1396
Fragments arranged by size:
2680 1396 1183 952 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2318 2966 5029 5216 1700
Size: 110 508 648 2063 187 2869
Fragments arranged by size:
2869 2063 648 508 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6385
BssHII G'CGCG_C
Cuts at: 1925 3131 3218 3390 3566 3639 1925
Size: 1206 87 172 176 73 4671
Fragments arranged by size:
4671 1206 176 172 87 73
BssSI C'ACGA_G
Cuts at: 4445 5457 4445
Size: 1012 5373
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2701 3304 3649 794
Size: 1907 603 345 3530
Fragments arranged by size:
3530 1907 603 345
BstBI TT'CG_AA
Cuts at: 5189 5189
Size: 6385
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6385
BstXI CCAn_nnnn'nTGG
Cuts at: 3127 3661 3127
Size: 534 5851
BstYI r'GATC_y
Cuts at: 1972 2950 3079 3328 3715 4082 4467 4484 5936
Size: 978 129 249 387 367 385 17 1452
Cuts at: 5936 6022 6034 6380 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 978 387 385 367 346 249
129 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2520 2520
Size: 6385
BtgI C'CryG_G
Cuts at: 513 1065 2659 2762 2835 3003 3113 3120 3550
Size: 552 1594 103 73 168 110 7 430
Cuts at: 3550 513
Size: 3348
Fragments arranged by size:
3348 1594 552 430 168 110 103 73
7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5646
Fragments arranged by size:
5646 683 56
BtsI GCAGTG_nn'
Cuts at: 2699 3060 3901 4695 4715 2699
Size: 361 841 794 20 4369
Fragments arranged by size:
4369 841 794 361 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6385
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6350
DraI TTT'AAA
Cuts at: 3689 3689
Size: 6385
DraIII CAC_nnn'GTG
Cuts at: 3526 3649 3672 3526
Size: 123 23 6239
Fragments arranged by size:
6239 123 23
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5392 809
Size: 507 4076 1802
Fragments arranged by size:
4076 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2295 2794 2964
Size: 54 1425 50 275 18 465 499 170
Cuts at: 2964 3066 3198 3663 3832 4721 6350 8
Size: 102 132 465 169 889 1629 43
Fragments arranged by size:
1629 1425 889 499 465 465 275 170
169 132 102 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3832 1537
Size: 275 2020 4090
Fragments arranged by size:
4090 2020 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4315 1353
Size: 2962 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4972 5346 5492 387
Size: 1064 138 3383 374 146 1280
Fragments arranged by size:
3383 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3403 3403
Size: 6385
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2127 2429 2484 2897 3032 3308 3557
Size: 393 495 302 55 413 135 276 249
Cuts at: 3557 4448 5045 5832 6100 1239
Size: 891 597 787 268 1524
Fragments arranged by size:
1524 891 787 597 495 413 393 302
276 268 249 135 55
EcoNI CCTnn'n_nnAGG
Cuts at: 2440 3623 2440
Size: 1183 5202
EcoO109I rG'GnC_Cy
Cuts at: 2011 2074 2271 2281 2282 2361 2374 2619 2911
Size: 63 197 10 1 79 13 245 292
Cuts at: 2911 2980 3245 3301 2011
Size: 69 265 56 5095
Fragments arranged by size:
5095 292 265 245 197 79 69 63
56 13 10 1
EcoRI G'AATT_C
Cuts at: 3694 4072 3694
Size: 378 6007
FspI TGC'GCA
Cuts at: 2790 4891 2790
Size: 2101 4284
HaeII r_GCGC'y
Cuts at: 1678 2238 2392 2492 2906 3011 3019 3113 3278
Size: 560 154 100 414 105 8 94 165
Cuts at: 3278 3296 3430 5532 1678
Size: 18 134 2102 2531
Fragments arranged by size:
2531 2102 560 414 165 154 134 105
100 94 18 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3089 3121 5026 5058 5100
Size: 32 135 32 1517 32 1905 32 42
Cuts at: 5100 5132 1373
Size: 32 2626
Fragments arranged by size:
2626 1905 1517 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2989 3729 3975 669
Size: 988 281 1051 740 246 3079
Fragments arranged by size:
3079 1051 988 740 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6385
HpaI GTT'AAC
Cuts at: 3975 3975
Size: 6385
KasI G'GCGC_C
Cuts at: 3109 3109
Size: 6385
KpnI G_GTAC'C
Cuts at: 3710 3710
Size: 6385
MfeI C'AATT_G
Cuts at: 3463 3984 3463
Size: 521 5864
MluI A'CGCG_T
Cuts at: 6261 6261
Size: 6385
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2976 3519 3603 5499 5683 1305
Size: 1671 543 84 1896 184 2007
Fragments arranged by size:
2007 1896 1671 543 184 84
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2297 3665 10
Size: 54 1425 343 465 1368 2730
Fragments arranged by size:
2730 1425 1368 465 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2735 4343 4702 4861 518
Size: 648 1569 1608 359 159 2042
Fragments arranged by size:
2042 1608 1569 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2096 2277 2661 2724 2764 2782 2800 2837
Size: 490 181 384 63 40 18 18 37
Cuts at: 2837 3005 3040 3211 3801 4478 5626 5871 1606
Size: 168 35 171 590 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 590 490 384 245 181
171 168 63 40 37 35 18 18
NaeI GCC'GGC
Cuts at: 2968 2968
Size: 6385
NarI GG'CG_CC
Cuts at: 3110 3110
Size: 6385
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5833
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6385
NgoMIV G'CCGG_C
Cuts at: 2966 2966
Size: 6385
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6385
NotI GC'GGCC_GC
Cuts at: 3832 3832
Size: 6385
NspI r_CATG'y
Cuts at: 1132 1229 3743 5288 1132
Size: 97 2514 1545 2229
Fragments arranged by size:
2514 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2050 2378 2507 1348
Size: 702 328 129 5226
Fragments arranged by size:
5226 702 328 129
PciI A'CATG_T
Cuts at: 5284 5284
Size: 6385
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6385
PfoI T'CCnGG_A
Cuts at: 2448 2874 2448
Size: 426 5959
PmeI GTTT'AAAC
Cuts at: 3689 3689
Size: 6385
PmlI CAC'GTG
Cuts at: 3523 3649 3523
Size: 126 6259
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4454 4486 5967 5999 4454
Size: 32 1481 32 4840
Fragments arranged by size:
4840 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2011 2374 2911 2011
Size: 363 537 5485
Fragments arranged by size:
5485 537 363
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6385
PsiI TTA'TAA
Cuts at: 3955 3955
Size: 6385
PspOMI G'GGCC_C
Cuts at: 2271 2281 2271
Size: 10 6375
PspXI vC'TCGA_Gb
Cuts at: 2843 2843
Size: 6385
PstI C_TGCA'G
Cuts at: 830 2655 2697 3300 3737 830
Size: 1825 42 603 437 3478
Fragments arranged by size:
3478 1825 603 437 42
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6385
PvuII CAG'CTG
Cuts at: 2096 2800 2096
Size: 704 5681
RsrII CG'GwC_CG
Cuts at: 2766 2766
Size: 6385
SacI G_AGCT'C
Cuts at: 3405 3405
Size: 6385
SacII CC_GC'GG
Cuts at: 2662 2765 2838 3006 2662
Size: 103 73 168 6041
Fragments arranged by size:
6041 168 103 73
SalI G'TCGA_C
Cuts at: 1936 3727 1936
Size: 1791 4594
SanDI GG'GwC_CC
Cuts at: 2374 2911 2374
Size: 537 5848
SbfI CC_TGCA'GG
Cuts at: 3737 3737
Size: 6385
ScaI AGT'ACT
Cuts at: 4633 6345 4633
Size: 1712 4673
SexAI A'CCwGG_T
Cuts at: 2589 2589
Size: 6385
SfcI C'TryA_G
Cuts at: 826 1045 2651 2693 3296 3733 4868 5549 5740
Size: 219 1606 42 603 437 1135 681 191
Cuts at: 5740 826
Size: 1471
Fragments arranged by size:
1606 1471 1135 681 603 437 219 191
42
SfiI GGCCn_nnn'nGGCC
Cuts at: 3252 3554 3252
Size: 302 6083
SfoI GGC'GCC
Cuts at: 3111 3111
Size: 6385
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6385
SgrAI Cr'CCGG_yG
Cuts at: 1700 2318 1700
Size: 618 5767
SmaI CCC'GGG
Cuts at: 1872 2924 3186 3712 1872
Size: 1052 262 526 4545
Fragments arranged by size:
4545 1052 526 262
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2843 4489 5390 5652 820
Size: 197 926 900 1646 901 262 1553
Fragments arranged by size:
1646 1553 926 901 900 262 197
SnaBI TAC'GTA
Cuts at: 493 4176 493
Size: 3683 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6385
SphI G_CATG'C
Cuts at: 1132 3743 1132
Size: 2611 3774
SrfI GCCC'GGGC
Cuts at: 2924 2924
Size: 6385
SspI AAT'ATT
Cuts at: 5 52 4309 5
Size: 47 4257 2081
Fragments arranged by size:
4257 2081 47
StuI AGG'CCT
Cuts at: 1517 2249 1517
Size: 732 5653
StyI C'CwwG_G
Cuts at: 513 1065 3824 513
Size: 552 2759 3074
Fragments arranged by size:
3074 2759 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2872 4412 4429 4582 4767 5212 325
Size: 1255 1292 1540 17 153 185 445 1498
Fragments arranged by size:
1540 1498 1292 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4631 6343 96
Size: 274 80 33 51 1443 2654 1712 138
Fragments arranged by size:
2654 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3064 3096 6345 6377 3064
Size: 32 3249 32 3072
Fragments arranged by size:
3249 3072 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6385
XbaI T'CTAG_A
Cuts at: 3721 3721
Size: 6385
XhoI C'TCGA_G
Cuts at: 1943 2843 1943
Size: 900 5485
XmaI C'CCGG_G
Cuts at: 1870 2922 3184 3710 1870
Size: 1052 262 526 4545
Fragments arranged by size:
4545 1052 526 262
XmnI GAAnn'nnTTC
Cuts at: 4514 4514
Size: 6385
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4193 276
Size: 53 83 186 936 2659 2468
Fragments arranged by size:
2659 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AvrII BaeI BfrBI BplI BsiWI BstZ17I
EcoRV FalI FseI FspAI NruI NsiI PacI PsrI
SapI SwaI XcmI