Restriction map for
pFN21AB5967
(Circular) (Six-base) MAPSORT of: pFN21AB5967.seq Check: 3230 from: 1 to: 6259
pFN21AB5967 6259 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4069 278
Size: 53 83 186 936 2533 2468
Fragments arranged by size:
2533 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3602 1937
Size: 1665 4594
Acc65I G'GTAC_C
Cuts at: 3580 3580
Size: 6259
AclI AA'CG_TT
Cuts at: 1563 4386 4759 1563
Size: 2823 373 3063
Fragments arranged by size:
3063 2823 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3434 4322 5706 5974 1632
Size: 1802 888 1384 268 1917
Fragments arranged by size:
1917 1802 1384 888 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6062
AflIII A'CryG_T
Cuts at: 1203 3201 5158 6135 1203
Size: 1998 1957 977 1327
Fragments arranged by size:
1998 1957 1327 977
AgeI A'CCGG_T
Cuts at: 5090 5090
Size: 6259
AhdI GACnn_n'nnGTC
Cuts at: 4988 4988
Size: 6259
AleI CACnn'nnGTG
Cuts at: 1166 3245 1166
Size: 2079 4180
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6259
AlwNI CAG_nnn'CTG
Cuts at: 3094 3231 4002 5574 3094
Size: 137 771 1572 3779
Fragments arranged by size:
3779 1572 771 137
ApaI G_GGCC'C
Cuts at: 2107 3306 2107
Size: 1199 5060
ApaLI G'TGCA_C
Cuts at: 2414 3518 4316 5472 2414
Size: 1104 798 1156 3201
Fragments arranged by size:
3201 1156 1104 798
ApoI r'AATT_y
Cuts at: 1493 2201 2348 2524 2961 3568 3794 3946 6055
Size: 708 147 176 437 607 226 152 2109
Cuts at: 6055 1493
Size: 1697
Fragments arranged by size:
2109 1697 708 607 437 226 176 152
147
AseI AT'TA_AT
Cuts at: 160 1032 4813 160
Size: 872 3781 1606
Fragments arranged by size:
3781 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6259
AvaI C'yCGr_G
Cuts at: 1870 1943 2087 2099 2838 3298 3584 1870
Size: 73 144 12 739 460 286 4545
Fragments arranged by size:
4545 739 460 286 144 73 12
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2956 2989 2956
Size: 33 6226
BamHI G'GATC_C
Cuts at: 3589 3956 3589
Size: 367 5892
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3098 3443 3535 3580 5035
Size: 325 217 627 1311 345 92 45 1455
Cuts at: 5035 618
Size: 1842
Fragments arranged by size:
1842 1455 1311 627 345 325 217 92
45
BanII G_rGCy'C
Cuts at: 1391 2107 2391 2845 3306 3578 1391
Size: 716 284 454 461 272 4072
Fragments arranged by size:
4072 716 461 454 284 272
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5288
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4450 4484 1903
Size: 34 2513 34 3678
Fragments arranged by size:
3678 2513 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4151 5361 4151
Size: 1210 5049
BclI T'GATC_A
Cuts at: 1548 2226 1548
Size: 678 5581
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2438 4870 136
Size: 107 122 71 631 605 766 2432 1525
Fragments arranged by size:
2432 1525 766 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6254 6254
Size: 6259
BlpI GC'TnA_GC
Cuts at: 2385 3676 2385
Size: 1291 4968
Bme1580I G_kGCm'C
Cuts at: 1790 2107 2219 2418 3177 3306 3522 4320 5476
Size: 317 112 199 759 129 216 798 1156
Cuts at: 5476 1790
Size: 2573
Fragments arranged by size:
2573 1156 798 759 317 216 199 129
112
BmgBI CAC'GTC
Cuts at: 1318 2617 1318
Size: 1299 4960
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2253 3039 3990
Size: 416 53 83 399 24 836 786 951
Cuts at: 3990 4948 442
Size: 958 1753
Fragments arranged by size:
1753 958 951 836 786 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6259
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2069 4919 1239
Size: 830 2850 2579
Fragments arranged by size:
2850 2579 830
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2462 4384 5249 5547 2462
Size: 1922 865 298 3174
Fragments arranged by size:
3174 1922 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4922 882
Size: 617 3423 2219
Fragments arranged by size:
3423 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2413 3204 4050 493
Size: 711 1209 791 846 2702
Fragments arranged by size:
2702 1209 846 791 711
BsaBI GATnn'nnATC
Cuts at: 6253 6253
Size: 6259
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4066 4448 5083 275
Size: 53 83 186 936 2533 382 635 1451
Fragments arranged by size:
2533 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4692 5090 5364 5511 5922 4692
Size: 398 274 147 411 5029
Fragments arranged by size:
5029 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2408 2438 3326 3356 3365
Size: 30 165 30 1005 30 888 30 9
Cuts at: 3365 3395 1178
Size: 30 4042
Fragments arranged by size:
4042 1005 888 165 30 30 30 30
30 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6259
BseYI C'CCAG_C
Cuts at: 2108 2145 3161 3176 3352 5462 5958 2108
Size: 37 1016 15 176 2110 496 2409
Fragments arranged by size:
2409 2110 1016 496 176 37 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2593 2720 3217 3269 1163
Size: 573 857 127 497 52 4153
Fragments arranged by size:
4153 857 573 497 127 52
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3709 4470 5498 1540
Size: 275 186 1708 761 1028 2301
Fragments arranged by size:
2301 1708 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2418 2789 2845 3522 4320 4405 5476 2418
Size: 371 56 677 798 85 1071 3201
Fragments arranged by size:
3201 1071 798 677 371 85 56
BsmI GAATG_Cn'
Cuts at: 2757 3770 3863 2757
Size: 1013 93 5153
Fragments arranged by size:
5153 1013 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2467 2467
Size: 6259
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2107 2219 2391 2418 2789 2845 3177
Size: 399 317 112 172 27 371 56 332
Cuts at: 3177 3306 3522 3578 4320 4405 5476 1391
Size: 129 216 56 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 371 332 317 216
172 129 112 85 56 56 27
BspEI T'CCGG_A
Cuts at: 5922 5922
Size: 6259
BspHI T'CATG_A
Cuts at: 4148 5878 4148
Size: 1730 4529
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2076 2335 3614 844
Size: 1232 259 1279 3489
Fragments arranged by size:
3489 1279 1232 259
BsrBI CCG'CTC
Cuts at: 1394 1823 3069 3287 4146 1394
Size: 429 1246 218 859 3507
Fragments arranged by size:
3507 1246 859 429 218
BsrDI GCAATG_nn'
Cuts at: 65 1248 4754 4928 65
Size: 1183 3506 174 1396
Fragments arranged by size:
3506 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4903 5090 1700
Size: 110 3093 187 2869
Fragments arranged by size:
3093 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6259
BssHII G'CGCG_C
Cuts at: 1925 2094 1925
Size: 169 6090
BssSI C'ACGA_G
Cuts at: 4319 5331 4319
Size: 1012 5247
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6259
BstBI TT'CG_AA
Cuts at: 2081 5063 2081
Size: 2982 3277
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6259
BstYI r'GATC_y
Cuts at: 1972 3589 3956 4341 4358 5810 5896 5908 6254
Size: 1617 367 385 17 1452 86 12 346
Cuts at: 6254 1972
Size: 1977
Fragments arranged by size:
1977 1617 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2772 3255 3337 3384 513
Size: 552 939 768 483 82 47 3388
Fragments arranged by size:
3388 939 768 552 483 82 47
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5520
Fragments arranged by size:
5520 683 56
BtsI GCAGTG_nn'
Cuts at: 2649 2725 2939 3416 3775 4569 4589 2649
Size: 76 214 477 359 794 20 4319
Fragments arranged by size:
4319 794 477 359 214 76 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6259
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6224
DraI TTT'AAA
Cuts at: 3563 3563
Size: 6259
DraIII CAC_nnn'GTG
Cuts at: 2212 2560 3047 3517 2212
Size: 348 487 470 4954
Fragments arranged by size:
4954 487 470 348
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5266 809
Size: 507 3950 1802
Fragments arranged by size:
3950 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2401 3206
Size: 54 1425 50 275 18 177 394 805
Cuts at: 3206 3330 3706 4595 6224 8
Size: 124 376 889 1629 43
Fragments arranged by size:
1629 1425 889 805 394 376 275 177
124 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3706 1537
Size: 275 1894 4090
Fragments arranged by size:
4090 1894 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4189 1353
Size: 2836 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2263 4846 5220 5366 387
Size: 1064 138 674 2583 374 146 1280
Fragments arranged by size:
2583 1280 1064 674 374 146 138
EcoICRI GAG'CTC
Cuts at: 2843 2843
Size: 6259
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2069 3434 4322 4919 5706 5974 1239
Size: 393 437 1365 888 597 787 268 1524
Fragments arranged by size:
1524 1365 888 787 597 437 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3106 3106
Size: 6259
EcoO109I rG'GnC_Cy
Cuts at: 2103 2104 2868 2911 3091 3302 3303 2103
Size: 1 764 43 180 211 1 5059
Fragments arranged by size:
5059 764 211 180 43 1 1
EcoRI G'AATT_C
Cuts at: 3568 3946 3568
Size: 378 5881
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3234 3234
Size: 6259
FspI TGC'GCA
Cuts at: 4765 4765
Size: 6259
HaeII r_GCGC'y
Cuts at: 1678 5406 1678
Size: 3728 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2541 2573 4900 4932 4974
Size: 32 135 32 969 32 2327 32 42
Cuts at: 4974 5006 1373
Size: 32 2626
Fragments arranged by size:
2626 2327 969 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3603 3849 669
Size: 988 281 1665 246 3079
Fragments arranged by size:
3079 1665 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3132 748
Size: 2384 3875
HpaI GTT'AAC
Cuts at: 3849 3849
Size: 6259
KpnI G_GTAC'C
Cuts at: 3584 3584
Size: 6259
MfeI C'AATT_G
Cuts at: 3858 3858
Size: 6259
MluI A'CGCG_T
Cuts at: 6135 6135
Size: 6259
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2835 5373 5557 1305
Size: 1530 2538 184 2007
Fragments arranged by size:
2538 2007 1530 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3208 3332 10
Size: 54 1425 343 1376 124 2937
Fragments arranged by size:
2937 1425 1376 343 124 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2222 3245 4217 4576 4735 518
Size: 648 1056 1023 972 359 159 2042
Fragments arranged by size:
2042 1056 1023 972 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3127 3195 3231 3352 3675 4352 5500 5745
Size: 1521 68 36 121 323 677 1148 245
Cuts at: 5745 1606
Size: 2120
Fragments arranged by size:
2120 1521 1148 677 323 245 121 68
36
NcoI C'CATG_G
Cuts at: 513 1065 2004 3255 3337 513
Size: 552 939 1251 82 3435
Fragments arranged by size:
3435 1251 939 552 82
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6259
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6259
NotI GC'GGCC_GC
Cuts at: 3706 3706
Size: 6259
NspI r_CATG'y
Cuts at: 1132 1229 3142 3217 3617 5162 1132
Size: 97 1913 75 400 1545 2229
Fragments arranged by size:
2229 1913 1545 400 97 75
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6259
PciI A'CATG_T
Cuts at: 5158 5158
Size: 6259
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2212 3400 1108
Size: 1104 1188 3967
Fragments arranged by size:
3967 1188 1104
PmeI GTTT'AAAC
Cuts at: 3563 3563
Size: 6259
PmlI CAC'GTG
Cuts at: 3204 3204
Size: 6259
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3350 3382 4328 4360 5841 5873 3350
Size: 32 946 32 1481 32 3736
Fragments arranged by size:
3736 1481 946 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2868 2911 2868
Size: 43 6216
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6259
PsiI TTA'TAA
Cuts at: 3829 3829
Size: 6259
PspOMI G'GGCC_C
Cuts at: 2103 3302 2103
Size: 1199 5060
PstI C_TGCA'G
Cuts at: 830 3435 3611 830
Size: 2605 176 3478
Fragments arranged by size:
3478 2605 176
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6259
PvuII CAG'CTG
Cuts at: 3195 3231 3352 3195
Size: 36 121 6102
Fragments arranged by size:
6102 121 36
SacI G_AGCT'C
Cuts at: 2845 2845
Size: 6259
SalI G'TCGA_C
Cuts at: 1936 3601 1936
Size: 1665 4594
SanDI GG'GwC_CC
Cuts at: 2868 2868
Size: 6259
SbfI CC_TGCA'GG
Cuts at: 3611 3611
Size: 6259
ScaI AGT'ACT
Cuts at: 4507 6219 4507
Size: 1712 4547
SfcI C'TryA_G
Cuts at: 826 1045 3106 3223 3431 3607 4742 5423 5614
Size: 219 2061 117 208 176 1135 681 191
Cuts at: 5614 826
Size: 1471
Fragments arranged by size:
2061 1471 1135 681 219 208 191 176
117
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6259
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6259
SmaI CCC'GGG
Cuts at: 1872 3586 1872
Size: 1714 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2477 2838 4363 5264 5526 820
Size: 197 926 534 361 1525 901 262 1553
Fragments arranged by size:
1553 1525 926 901 534 361 262 197
SnaBI TAC'GTA
Cuts at: 493 4050 493
Size: 3557 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6259
SphI G_CATG'C
Cuts at: 1132 3217 3617 1132
Size: 2085 400 3774
Fragments arranged by size:
3774 2085 400
SspI AAT'ATT
Cuts at: 5 52 4183 5
Size: 47 4131 2081
Fragments arranged by size:
4131 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6259
StyI C'CwwG_G
Cuts at: 513 1065 2004 2235 3255 3337 3485 3698 513
Size: 552 939 231 1020 82 148 213 3074
Fragments arranged by size:
3074 1020 939 552 231 213 148 82
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2159 2433 2941 3058 3086 4286 4303
Size: 1255 579 274 508 117 28 1200 17
Cuts at: 4303 4456 4641 5086 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1200 579 508 445 274 185
153 117 28 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2301 4505 6217
Size: 274 80 33 51 1443 324 2204 1712
Cuts at: 6217 96
Size: 138
Fragments arranged by size:
2204 1712 1443 324 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2154 2186 2408 2440 2995 3027 3365 3397 6219
Size: 32 222 32 555 32 338 32 2822
Cuts at: 6219 6251 2154
Size: 32 2162
Fragments arranged by size:
2822 2162 555 338 222 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3115 1316
Size: 1799 4460
XbaI T'CTAG_A
Cuts at: 3595 3595
Size: 6259
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2494 2885 2494
Size: 391 5868
XhoI C'TCGA_G
Cuts at: 1943 2838 1943
Size: 895 5364
XmaI C'CCGG_G
Cuts at: 1870 3584 1870
Size: 1714 4545
XmnI GAAnn'nnTTC
Cuts at: 4388 4388
Size: 6259
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4067 276
Size: 53 83 186 936 2533 2468
Fragments arranged by size:
2533 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PspXI PsrI RsrII SacII SapI SexAI SfiI
SfoI SrfI SwaI