Restriction map for
pFN21AB5965
(Circular) (Six-base) MAPSORT of: pFN21AB5965.seq Check: 7299 from: 1 to: 6433
pFN21AB5965 6433 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3314 3314
Size: 6433
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4243 278
Size: 53 83 186 936 2707 2468
Fragments arranged by size:
2707 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2288 2833 3272 3776 1937
Size: 351 545 439 504 4594
Fragments arranged by size:
4594 545 504 439 351
Acc65I G'GTAC_C
Cuts at: 3754 3754
Size: 6433
AclI AA'CG_TT
Cuts at: 1563 4560 4933 1563
Size: 2997 373 3063
Fragments arranged by size:
3063 2997 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2885 3368 4496 5880 6148 1632
Size: 1253 483 1128 1384 268 1917
Fragments arranged by size:
1917 1384 1253 1128 483 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6236
AflIII A'CryG_T
Cuts at: 1203 5332 6309 1203
Size: 4129 977 1327
Fragments arranged by size:
4129 1327 977
AgeI A'CCGG_T
Cuts at: 5264 5264
Size: 6433
AhdI GACnn_n'nnGTC
Cuts at: 2212 5162 2212
Size: 2950 3483
AleI CACnn'nnGTG
Cuts at: 1166 3108 3628 1166
Size: 1942 520 3971
Fragments arranged by size:
3971 1942 520
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6433
AlwNI CAG_nnn'CTG
Cuts at: 2171 4176 5748 2171
Size: 2005 1572 2856
Fragments arranged by size:
2856 2005 1572
ApaLI G'TGCA_C
Cuts at: 3129 3263 4490 5646 3129
Size: 134 1227 1156 3916
Fragments arranged by size:
3916 1227 1156 134
ApoI r'AATT_y
Cuts at: 1493 3742 3968 4120 6229 1493
Size: 2249 226 152 2109 1697
Fragments arranged by size:
2249 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4987 160
Size: 872 3955 1606
Fragments arranged by size:
3955 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6433
AvaI C'yCGr_G
Cuts at: 1870 1943 2577 2618 3022 3758 1870
Size: 73 634 41 404 736 4545
Fragments arranged by size:
4545 736 634 404 73 41
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2998 3031 2998
Size: 33 6400
BamHI G'GATC_C
Cuts at: 2916 3763 4130 2916
Size: 847 367 5219
Fragments arranged by size:
5219 847 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2691 3754 5209 618
Size: 325 217 627 904 1063 1455 1842
Fragments arranged by size:
1842 1455 1063 904 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3752 1391
Size: 2361 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5462
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4624 4658 1903
Size: 34 2687 34 3678
Fragments arranged by size:
3678 2687 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3026 4325 5535 3026
Size: 1299 1210 3924
Fragments arranged by size:
3924 1299 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6433
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3497 5044 136
Size: 107 122 71 631 605 1825 1547 1525
Fragments arranged by size:
1825 1547 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6428 6428
Size: 6433
BlpI GC'TnA_GC
Cuts at: 2847 3850 2847
Size: 1003 5430
Bme1580I G_kGCm'C
Cuts at: 1790 2780 2963 3133 3267 4494 5650 1790
Size: 990 183 170 134 1227 1156 2573
Fragments arranged by size:
2573 1227 1156 990 183 170 134
BmgBI CAC'GTC
Cuts at: 1318 2376 1318
Size: 1058 5375
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3622 4164 5122
Size: 416 53 83 399 24 2205 542 958
Cuts at: 5122 442
Size: 1753
Fragments arranged by size:
2205 1753 958 542 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6433
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2328 3068 5093 1239
Size: 1089 740 2025 2579
Fragments arranged by size:
2579 2025 1089 740
Bpu10I CC'TnA_GC
Cuts at: 2568 3456 3462 2568
Size: 888 6 5539
Fragments arranged by size:
5539 888 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3423 3429 3453 4558 5423 5721 3423
Size: 6 24 1105 865 298 4135
Fragments arranged by size:
4135 1105 865 298 24 6
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2753 2933 5096 882
Size: 617 1254 180 2163 2219
Fragments arranged by size:
2219 2163 1254 617 180
BsaAI yAC'GTr
Cuts at: 493 1204 4224 493
Size: 711 3020 2702
Fragments arranged by size:
3020 2702 711
BsaBI GATnn'nnATC
Cuts at: 2664 2735 6427 2664
Size: 71 3692 2670
Fragments arranged by size:
3692 2670 71
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4240 4622 5257 275
Size: 53 83 186 936 2707 382 635 1451
Fragments arranged by size:
2707 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4866 5264 5538 5685 6096 4866
Size: 398 274 147 411 5203
Fragments arranged by size:
5203 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6208
Fragments arranged by size:
6208 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6433
BseYI C'CCAG_C
Cuts at: 2820 3003 5636 6132 2820
Size: 183 2633 496 3121
Fragments arranged by size:
3121 2633 496 183
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3292 3416 1163
Size: 573 1556 124 4180
Fragments arranged by size:
4180 1556 573 124
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3883 4644 5672 1540
Size: 275 186 1882 761 1028 2301
Fragments arranged by size:
2301 1882 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2705 3133 3267 3720 4494 4579 5650 2705
Size: 428 134 453 774 85 1071 3488
Fragments arranged by size:
3488 1071 774 453 428 134 85
BsmI GAATG_Cn'
Cuts at: 3944 4037 3944
Size: 93 6340
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2705 2780 2963 3133 3267 3720 3752
Size: 399 915 75 183 170 134 453 32
Cuts at: 3752 4494 4579 5650 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 915 742 453 399 183 170
134 85 75 32
BspEI T'CCGG_A
Cuts at: 6096 6096
Size: 6433
BspHI T'CATG_A
Cuts at: 4322 6052 4322
Size: 1730 4703
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2470 3314 3788 844
Size: 1626 844 474 3489
Fragments arranged by size:
3489 1626 844 474
BsrBI CCG'CTC
Cuts at: 1394 1823 4320 1394
Size: 429 2497 3507
Fragments arranged by size:
3507 2497 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2782 4928 5102 65
Size: 1183 1534 2146 174 1396
Fragments arranged by size:
2146 1534 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5077 5264 1700
Size: 110 3267 187 2869
Fragments arranged by size:
3267 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3300 96
Size: 3204 3229
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6433
BssSI C'ACGA_G
Cuts at: 4493 5505 4493
Size: 1012 5421
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3315 3394 794
Size: 2521 79 3833
Fragments arranged by size:
3833 2521 79
BstBI TT'CG_AA
Cuts at: 5237 5237
Size: 6433
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6433
BstXI CCAn_nnnn'nTGG
Cuts at: 3572 3712 3572
Size: 140 6293
BstYI r'GATC_y
Cuts at: 1972 2736 2916 3763 4130 4515 4532 5984 6070
Size: 764 180 847 367 385 17 1452 86
Cuts at: 6070 6082 6428 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 847 764 385 367 346 180
86 17 12
BstZ17I GTA'TAC
Cuts at: 2834 2834
Size: 6433
Bsu36I CC'TnA_GG
Cuts at: 2191 2383 2649 2191
Size: 192 266 5975
Fragments arranged by size:
5975 266 192
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4942
Fragments arranged by size:
4942 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5694
Fragments arranged by size:
5694 683 56
BtsI GCAGTG_nn'
Cuts at: 2563 2965 3426 3949 4743 4763 2563
Size: 402 461 523 794 20 4233
Fragments arranged by size:
4233 794 523 461 402 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6433
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6398
DraI TTT'AAA
Cuts at: 3737 3737
Size: 6433
DraIII CAC_nnn'GTG
Cuts at: 3283 3283
Size: 6433
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2636 5440 809
Size: 507 1320 2804 1802
Fragments arranged by size:
2804 1802 1320 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3723 3880 4769
Size: 54 1425 50 275 18 1893 157 889
Cuts at: 4769 6398 8
Size: 1629 43
Fragments arranged by size:
1893 1629 1425 889 275 157 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3880 1537
Size: 275 2068 4090
Fragments arranged by size:
4090 2068 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2329 2460 4363 1353
Size: 976 131 1903 3423
Fragments arranged by size:
3423 1903 976 131
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2550 2873 3056 3677 5020 5394
Size: 1064 138 961 323 183 621 1343 374
Cuts at: 5394 5540 387
Size: 146 1280
Fragments arranged by size:
1343 1280 1064 961 621 374 323 183
146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2328 2885 3068 3368 4496 5093 5880
Size: 393 696 557 183 300 1128 597 787
Cuts at: 5880 6148 1239
Size: 268 1524
Fragments arranged by size:
1524 1128 787 696 597 557 393 300
268 183
EcoNI CCTnn'n_nnAGG
Cuts at: 2522 2522
Size: 6433
EcoRI G'AATT_C
Cuts at: 3742 4120 3742
Size: 378 6055
FspI TGC'GCA
Cuts at: 3362 4939 3362
Size: 1577 4856
HaeII r_GCGC'y
Cuts at: 1678 5580 1678
Size: 3902 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2089 2121 2198 2204 2230
Size: 32 135 32 517 32 77 6 26
Cuts at: 2230 2236 3236 3268 5074 5106 5148 5180 1373
Size: 6 1000 32 1806 32 42 32 2626
Fragments arranged by size:
2626 1806 1000 517 135 77 42 32
32 32 32 32 32 26 6 6
HincII GTy'rAC
Cuts at: 669 1657 1938 3777 4023 669
Size: 988 281 1839 246 3079
Fragments arranged by size:
3079 1839 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6433
HpaI GTT'AAC
Cuts at: 4023 4023
Size: 6433
KpnI G_GTAC'C
Cuts at: 3758 3758
Size: 6433
MfeI C'AATT_G
Cuts at: 4032 4032
Size: 6433
MluI A'CGCG_T
Cuts at: 6309 6309
Size: 6433
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2706 5547 5731 1305
Size: 1401 2841 184 2007
Fragments arranged by size:
2841 2007 1401 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3725 10
Size: 54 1425 343 1893 2718
Fragments arranged by size:
2718 1893 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2201 3108 3124 3628 3710 4391 4750
Size: 648 1035 907 16 504 82 681 359
Cuts at: 4750 4909 518
Size: 159 2042
Fragments arranged by size:
2042 1035 907 681 648 504 359 159
82 16
MspA1I CmG'CkG
Cuts at: 1606 2110 2230 3413 3849 4526 5674 5919 1606
Size: 504 120 1183 436 677 1148 245 2120
Fragments arranged by size:
2120 1183 1148 677 504 436 245 120
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4942
Fragments arranged by size:
4942 939 552
NdeI CA'TA_TG
Cuts at: 387 3357 387
Size: 2970 3463
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6433
NotI GC'GGCC_GC
Cuts at: 3880 3880
Size: 6433
NspI r_CATG'y
Cuts at: 1132 1229 3293 3791 5336 1132
Size: 97 2064 498 1545 2229
Fragments arranged by size:
2229 2064 1545 498 97
PasI CC'CwG_GG
Cuts at: 1348 3586 1348
Size: 2238 4195
PciI A'CATG_T
Cuts at: 5332 5332
Size: 6433
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6433
PmeI GTTT'AAAC
Cuts at: 3737 3737
Size: 6433
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4502 4534 6015 6047 4502
Size: 32 1481 32 4888
Fragments arranged by size:
4888 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6433
PsiI TTA'TAA
Cuts at: 4003 4003
Size: 6433
PstI C_TGCA'G
Cuts at: 830 2481 3177 3320 3785 830
Size: 1651 696 143 465 3478
Fragments arranged by size:
3478 1651 696 465 143
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6433
PvuII CAG'CTG
Cuts at: 2110 2230 2110
Size: 120 6313
SalI G'TCGA_C
Cuts at: 1936 3775 1936
Size: 1839 4594
SapI GCTCTTCn'nnn_
Cuts at: 2460 2460
Size: 6433
SbfI CC_TGCA'GG
Cuts at: 2481 3785 2481
Size: 1304 5129
ScaI AGT'ACT
Cuts at: 4681 6393 4681
Size: 1712 4721
SfcI C'TryA_G
Cuts at: 826 1045 2289 2477 2686 2854 3173 3316 3781
Size: 219 1244 188 209 168 319 143 465
Cuts at: 3781 4916 5597 5788 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1244 1135 681 465 319 219 209
191 188 168 143
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6433
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6433
SmaI CCC'GGG
Cuts at: 1872 2579 3760 1872
Size: 707 1181 4545
Fragments arranged by size:
4545 1181 707
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3438 3444 3468 4537 5438 5700
Size: 197 926 1495 6 24 1069 901 262
Cuts at: 5700 820
Size: 1553
Fragments arranged by size:
1553 1495 1069 926 901 262 197 24
6
SnaBI TAC'GTA
Cuts at: 493 4224 493
Size: 3731 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6433
SphI G_CATG'C
Cuts at: 1132 3293 3791 1132
Size: 2161 498 3774
Fragments arranged by size:
3774 2161 498
SspI AAT'ATT
Cuts at: 5 52 4357 5
Size: 47 4305 2081
Fragments arranged by size:
4305 2081 47
StuI AGG'CCT
Cuts at: 1517 3062 3491 1517
Size: 1545 429 4459
Fragments arranged by size:
4459 1545 429
StyI C'CwwG_G
Cuts at: 513 1065 2004 3294 3872 513
Size: 552 939 1290 578 3074
Fragments arranged by size:
3074 1290 939 578 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3150 4460 4477 4630 4815 5260 325
Size: 1255 1570 1310 17 153 185 445 1498
Fragments arranged by size:
1570 1498 1310 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3300 4679 6391
Size: 274 80 33 51 1443 1323 1379 1712
Cuts at: 6391 96
Size: 138
Fragments arranged by size:
1712 1443 1379 1323 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3546 3578 6393 6425 3546
Size: 32 2815 32 3554
Fragments arranged by size:
3554 2815 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6433
XbaI T'CTAG_A
Cuts at: 3769 3769
Size: 6433
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3593 3593
Size: 6433
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6433
XmaI C'CCGG_G
Cuts at: 1870 2577 3758 1870
Size: 707 1181 4545
Fragments arranged by size:
4545 1181 707
XmnI GAAnn'nnTTC
Cuts at: 2879 3062 3152 4562 2879
Size: 183 90 1410 4750
Fragments arranged by size:
4750 1410 183 90
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4241 276
Size: 53 83 186 936 2707 2468
Fragments arranged by size:
2707 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI BsiWI BsmBI EcoICRI EcoO109I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI