Restriction map for
pFN21AB5959
(Circular) (Six-base) MAPSORT of: pFN21AB5959.seq Check: 9599 from: 1 to: 6244
pFN21AB5959 6244 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2209 3385 2209
Size: 1176 5068
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2531 4054 278
Size: 53 83 186 936 995 1523 2468
Fragments arranged by size:
2468 1523 995 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3587 1937
Size: 1650 4594
Acc65I G'GTAC_C
Cuts at: 3565 3565
Size: 6244
AclI AA'CG_TT
Cuts at: 1563 4371 4744 1563
Size: 2808 373 3063
Fragments arranged by size:
3063 2808 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2198 3008 3337 4307 5691 5959 1632
Size: 566 810 329 970 1384 268 1917
Fragments arranged by size:
1917 1384 970 810 566 329 268
AfeI AGC'GCT
Cuts at: 3533 3533
Size: 6244
AflII C'TTAA_G
Cuts at: 820 1017 2189 820
Size: 197 1172 4875
Fragments arranged by size:
4875 1172 197
AflIII A'CryG_T
Cuts at: 1203 2902 5143 6120 1203
Size: 1699 2241 977 1327
Fragments arranged by size:
2241 1699 1327 977
AgeI A'CCGG_T
Cuts at: 5075 5075
Size: 6244
AhdI GACnn_n'nnGTC
Cuts at: 4973 4973
Size: 6244
AleI CACnn'nnGTG
Cuts at: 1166 2667 2978 1166
Size: 1501 311 4432
Fragments arranged by size:
4432 1501 311
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2891 1184
Size: 1707 4537
AlwNI CAG_nnn'CTG
Cuts at: 2341 2434 3041 3197 3241 3377 3987 5559 2341
Size: 93 607 156 44 136 610 1572 3026
Fragments arranged by size:
3026 1572 610 607 156 136 93 44
ApaI G_GGCC'C
Cuts at: 2088 2393 3421 2088
Size: 305 1028 4911
Fragments arranged by size:
4911 1028 305
ApaLI G'TGCA_C
Cuts at: 2272 3499 4301 5457 2272
Size: 1227 802 1156 3059
Fragments arranged by size:
3059 1227 1156 802
ApoI r'AATT_y
Cuts at: 1493 3553 3779 3931 6040 1493
Size: 2060 226 152 2109 1697
Fragments arranged by size:
2109 2060 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4798 160
Size: 872 3766 1606
Fragments arranged by size:
3766 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6244
AvaI C'yCGr_G
Cuts at: 1870 1943 2055 2206 2564 3414 3441 3569 1870
Size: 73 112 151 358 850 27 128 4545
Fragments arranged by size:
4545 850 358 151 128 112 73 27
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2248 2281 2248
Size: 33 6211
BamHI G'GATC_C
Cuts at: 3574 3941 3574
Size: 367 5877
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2404 3029 3428 3565 5020
Size: 325 217 627 617 625 399 137 1455
Cuts at: 5020 618
Size: 1842
Fragments arranged by size:
1842 1455 627 625 617 399 325 217
137
BanII G_rGCy'C
Cuts at: 1391 2056 2088 2393 2421 2428 3181 3363 3421
Size: 665 32 305 28 7 753 182 58
Cuts at: 3421 3563 1391
Size: 142 4072
Fragments arranged by size:
4072 753 665 305 182 142 58 32
28 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5273
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4435 4469 1903
Size: 34 2498 34 3678
Fragments arranged by size:
3678 2498 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4136 5346 4136
Size: 1210 5034
BclI T'GATC_A
Cuts at: 1548 2758 2941 1548
Size: 1210 183 4851
Fragments arranged by size:
4851 1210 183
BfrBI ATG'CAT
Cuts at: 3230 3230
Size: 6244
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4855 136
Size: 107 122 71 631 605 3183 1525
Fragments arranged by size:
3183 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6239 6239
Size: 6244
BlpI GC'TnA_GC
Cuts at: 3347 3661 3347
Size: 314 5930
Bme1580I G_kGCm'C
Cuts at: 1790 2088 2276 2393 3421 3503 4305 5461 1790
Size: 298 188 117 1028 82 802 1156 2573
Fragments arranged by size:
2573 1156 1028 802 298 188 117 82
BmgBI CAC'GTC
Cuts at: 1318 2905 1318
Size: 1587 4657
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2064 3029 3121
Size: 416 53 83 399 24 647 965 92
Cuts at: 3121 3254 3975 4933 442
Size: 133 721 958 1753
Fragments arranged by size:
1753 965 958 721 647 416 399 133
92 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6244
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2104 2104
Size: 6244
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2351 2435 2437 2686 4904 1239
Size: 1112 84 2 249 2218 2579
Fragments arranged by size:
2579 2218 1112 249 84 2
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4369 5234 5532 4369
Size: 865 298 5081
Fragments arranged by size:
5081 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2765 4907 882
Size: 617 1266 2142 2219
Fragments arranged by size:
2219 2142 1266 617
BsaAI yAC'GTr
Cuts at: 493 1204 4035 493
Size: 711 2831 2702
Fragments arranged by size:
2831 2702 711
BsaBI GATnn'nnATC
Cuts at: 6238 6238
Size: 6244
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2026 2528 4051 4433
Size: 53 83 186 936 493 502 1523 382
Cuts at: 4433 5068 275
Size: 635 1451
Fragments arranged by size:
1523 1451 936 635 502 493 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3328 3333 4677 5075 5349 5496 5907 3328
Size: 5 1344 398 274 147 411 3665
Fragments arranged by size:
3665 1344 411 398 274 147 5
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2043 2073 2354 2384 2665
Size: 30 165 30 640 30 281 30 281
Cuts at: 2665 2695 1178
Size: 30 4727
Fragments arranged by size:
4727 640 281 281 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2378 2972 1186
Size: 1192 594 4458
Fragments arranged by size:
4458 1192 594
BseYI C'CCAG_C
Cuts at: 2133 2198 3074 3291 3363 5447 5943 2133
Size: 65 876 217 72 2084 496 2434
Fragments arranged by size:
2434 2084 876 496 217 72 65
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3002 3101 1163
Size: 573 1266 99 4306
Fragments arranged by size:
4306 1266 573 99
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3344 3694 4455 5483 1540
Size: 275 186 1343 350 761 1028 2301
Fragments arranged by size:
2301 1343 1028 761 350 275 186
BsiHKAI G_wGCw'C
Cuts at: 2276 3486 3503 4305 4390 5461 2276
Size: 1210 17 802 85 1071 3059
Fragments arranged by size:
3059 1210 1071 802 85 17
BsmI GAATG_Cn'
Cuts at: 3755 3848 3755
Size: 93 6151
BsmBI CGTCTCn'nnnn_
Cuts at: 2455 2455
Size: 6244
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2056 2088 2276 2393 2421 2428 3181
Size: 399 266 32 188 117 28 7 753
Cuts at: 3181 3363 3421 3486 3503 3563 4305 4390 5461
Size: 182 58 65 17 60 742 85 1071
Cuts at: 5461 1391
Size: 2174
Fragments arranged by size:
2174 1071 753 742 399 266 188 182
117 85 65 60 58 32 28 17
7
BspEI T'CCGG_A
Cuts at: 3333 5907 3333
Size: 2574 3670
BspHI T'CATG_A
Cuts at: 4133 5863 4133
Size: 1730 4514
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2209 2335 3385 3599 844
Size: 1365 126 1050 214 3489
Fragments arranged by size:
3489 1365 1050 214 126
BsrBI CCG'CTC
Cuts at: 1394 1823 2129 4131 1394
Size: 429 306 2002 3507
Fragments arranged by size:
3507 2002 429 306
BsrDI GCAATG_nn'
Cuts at: 65 1248 4739 4913 65
Size: 1183 3491 174 1396
Fragments arranged by size:
3491 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3339 3510 4888 5075 1700
Size: 110 1529 171 1378 187 2869
Fragments arranged by size:
2869 1529 1378 187 171 110
BsrGI T'GTAC_A
Cuts at: 96 2551 2841 96
Size: 2455 290 3499
Fragments arranged by size:
3499 2455 290
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6244
BssSI C'ACGA_G
Cuts at: 4304 5316 4304
Size: 1012 5232
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2341 3227 3507 794
Size: 1547 886 280 3531
Fragments arranged by size:
3531 1547 886 280
BstBI TT'CG_AA
Cuts at: 5048 5048
Size: 6244
BstEII G'GTnAC_C
Cuts at: 1184 2314 1184
Size: 1130 5114
BstXI CCAn_nnnn'nTGG
Cuts at: 2728 2728
Size: 6244
BstYI r'GATC_y
Cuts at: 1972 3255 3574 3941 4326 4343 5795 5881 5893
Size: 1283 319 367 385 17 1452 86 12
Cuts at: 5893 6239 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1283 385 367 346 319 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3172 3172
Size: 6244
BtgI C'CryG_G
Cuts at: 513 1065 2004 2517 2609 513
Size: 552 939 513 92 4148
Fragments arranged by size:
4148 939 552 513 92
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3395 487
Size: 683 56 2169 3336
Fragments arranged by size:
3336 2169 683 56
BtsI GCAGTG_nn'
Cuts at: 2111 2496 2683 3760 4554 4574 2111
Size: 385 187 1077 794 20 3781
Fragments arranged by size:
3781 1077 794 385 187 20
ClaI AT'CG_AT
Cuts at: 1554 2463 1554
Size: 909 5335
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6209
DraI TTT'AAA
Cuts at: 3548 3548
Size: 6244
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2533 5251 809
Size: 507 1217 2718 1802
Fragments arranged by size:
2718 1802 1217 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2514 2855 3691
Size: 54 1425 50 275 18 684 341 836
Cuts at: 3691 4580 6209 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 836 684 341 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3691 1537
Size: 275 1879 4090
Fragments arranged by size:
4090 1879 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3108 4174 1353
Size: 1755 1066 3423
Fragments arranged by size:
3423 1755 1066
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3298 4831 5205 5351 387
Size: 1064 138 1709 1533 374 146 1280
Fragments arranged by size:
1709 1533 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2198 2351 2435 2437 2686 3008 3337
Size: 393 566 153 84 2 249 322 329
Cuts at: 3337 4307 4904 5691 5959 1239
Size: 970 597 787 268 1524
Fragments arranged by size:
1524 970 787 597 566 393 329 322
268 249 153 84 2
EcoNI CCTnn'n_nnAGG
Cuts at: 2216 2216
Size: 6244
EcoO109I rG'GnC_Cy
Cuts at: 2066 2084 2085 2389 2390 2431 3201 2066
Size: 18 1 304 1 41 770 5109
Fragments arranged by size:
5109 770 304 41 18 1 1
EcoRI G'AATT_C
Cuts at: 3553 3931 3553
Size: 378 5866
FspI TGC'GCA
Cuts at: 4750 4750
Size: 6244
HaeII r_GCGC'y
Cuts at: 1678 3535 5391 1678
Size: 1857 1856 2531
Fragments arranged by size:
2531 1857 1856
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2293 2325 3373 3405 4885
Size: 32 135 32 721 32 1048 32 1480
Cuts at: 4885 4917 4959 4991 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1480 1048 721 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3588 3834 669
Size: 988 281 1650 246 3079
Fragments arranged by size:
3079 1650 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3349 748
Size: 2601 3643
HpaI GTT'AAC
Cuts at: 3834 3834
Size: 6244
KpnI G_GTAC'C
Cuts at: 3569 3569
Size: 6244
MfeI C'AATT_G
Cuts at: 3843 3843
Size: 6244
MluI A'CGCG_T
Cuts at: 6120 6120
Size: 6244
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2463 5358 5542 1305
Size: 1158 2895 184 2007
Fragments arranged by size:
2895 2007 1158 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2857 10
Size: 54 1425 343 1025 3397
Fragments arranged by size:
3397 1425 1025 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2667 2978 4202 4561 4720 518
Size: 648 1501 311 1224 359 159 2042
Fragments arranged by size:
2042 1501 1224 648 359 311 159
MspA1I CmG'CkG
Cuts at: 1606 2298 2640 2658 2986 3291 3660 4337 5485
Size: 692 342 18 328 305 369 677 1148
Cuts at: 5485 5730 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 692 677 369 342 328 305
245 18
NcoI C'CATG_G
Cuts at: 513 1065 2004 2517 2609 513
Size: 552 939 513 92 4148
Fragments arranged by size:
4148 939 552 513 92
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6244
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6244
NotI GC'GGCC_GC
Cuts at: 3691 3691
Size: 6244
NsiI A_TGCA'T
Cuts at: 3232 3232
Size: 6244
NspI r_CATG'y
Cuts at: 1132 1229 3602 5147 1132
Size: 97 2373 1545 2229
Fragments arranged by size:
2373 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3041 1348
Size: 1693 4551
PciI A'CATG_T
Cuts at: 5143 5143
Size: 6244
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2434 2864 3041 1108
Size: 1326 430 177 4311
Fragments arranged by size:
4311 1326 430 177
PfoI T'CCnGG_A
Cuts at: 2453 2453
Size: 6244
PmeI GTTT'AAAC
Cuts at: 3548 3548
Size: 6244
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4313 4345 5826 5858 4313
Size: 32 1481 32 4699
Fragments arranged by size:
4699 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2066 3201 2066
Size: 1135 5109
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6244
PsiI TTA'TAA
Cuts at: 3814 3814
Size: 6244
PspOMI G'GGCC_C
Cuts at: 2084 2389 3417 2084
Size: 305 1028 4911
Fragments arranged by size:
4911 1028 305
PstI C_TGCA'G
Cuts at: 830 2346 2357 2388 2543 3596 830
Size: 1516 11 31 155 1053 3478
Fragments arranged by size:
3478 1516 1053 155 31 11
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6244
PvuII CAG'CTG
Cuts at: 2298 2986 3291 2298
Size: 688 305 5251
Fragments arranged by size:
5251 688 305
RsrII CG'GwC_CG
Cuts at: 3331 3331
Size: 6244
SalI G'TCGA_C
Cuts at: 1936 3586 1936
Size: 1650 4594
SanDI GG'GwC_CC
Cuts at: 2066 2066
Size: 6244
SapI GCTCTTCn'nnn_
Cuts at: 3108 3108
Size: 6244
SbfI CC_TGCA'GG
Cuts at: 3596 3596
Size: 6244
ScaI AGT'ACT
Cuts at: 4492 6204 4492
Size: 1712 4532
SfcI C'TryA_G
Cuts at: 826 1045 2342 2353 2384 2440 2539 3592 4727
Size: 219 1297 11 31 56 99 1053 1135
Cuts at: 4727 5408 5599 826
Size: 681 191 1471
Fragments arranged by size:
1471 1297 1135 1053 681 219 191 99
56 31 11
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6244
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6244
SmaI CCC'GGG
Cuts at: 1872 2057 2566 3416 3571 1872
Size: 185 509 850 155 4545
Fragments arranged by size:
4545 850 509 185 155
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2189 4348 5249 5511 820
Size: 197 926 246 2159 901 262 1553
Fragments arranged by size:
2159 1553 926 901 262 246 197
SnaBI TAC'GTA
Cuts at: 493 4035 493
Size: 3542 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6244
SphI G_CATG'C
Cuts at: 1132 3602 1132
Size: 2470 3774
SspI AAT'ATT
Cuts at: 5 52 4168 5
Size: 47 4116 2081
Fragments arranged by size:
4116 2081 47
StuI AGG'CCT
Cuts at: 1517 3524 1517
Size: 2007 4237
StyI C'CwwG_G
Cuts at: 513 1065 2004 2029 2178 2517 2609 3683 513
Size: 552 939 25 149 339 92 1074 3074
Fragments arranged by size:
3074 1074 939 552 339 149 92 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2585 2789 4271 4288 4441 4626 5071
Size: 1255 1005 204 1482 17 153 185 445
Cuts at: 5071 325
Size: 1498
Fragments arranged by size:
1498 1482 1255 1005 445 204 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2551 2841 4490
Size: 274 80 33 51 1443 574 290 1649
Cuts at: 4490 6202 96
Size: 1712 138
Fragments arranged by size:
1712 1649 1443 574 290 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2043 2075 2354 2386 2605 2637 2665 2697 3482
Size: 32 279 32 219 32 28 32 785
Cuts at: 3482 3514 6204 6236 2043
Size: 32 2690 32 2051
Fragments arranged by size:
2690 2051 785 279 219 32 32 32
32 32 32 28
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6244
XbaI T'CTAG_A
Cuts at: 3580 3580
Size: 6244
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2759 2759
Size: 6244
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6244
XmaI C'CCGG_G
Cuts at: 1870 2055 2564 3414 3569 1870
Size: 185 509 850 155 4545
Fragments arranged by size:
4545 850 509 185 155
XmnI GAAnn'nnTTC
Cuts at: 4373 4373
Size: 6244
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2529 4052 276
Size: 53 83 186 936 995 1523 2468
Fragments arranged by size:
2468 1523 995 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII RsrII SalI SanDI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BbeI BbvCI Bpu10I BsiWI BstZ17I
DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PspXI PsrI SacI
SacII SexAI SfiI SfoI SrfI SwaI