Restriction map for
pFN21AB5958
(Circular) (Six-base) MAPSORT of: pFN21AB5958.seq Check: 9597 from: 1 to: 6355
pFN21AB5958 6355 bp
With 182 enzymes: SgfI *
March 28, 2012 12:02 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4165 278
Size: 53 83 186 936 2629 2468
Fragments arranged by size:
2629 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2447 3698 1937
Size: 510 1251 4594
Fragments arranged by size:
4594 1251 510
Acc65I G'GTAC_C
Cuts at: 3676 3676
Size: 6355
AclI AA'CG_TT
Cuts at: 1563 4482 4855 1563
Size: 2919 373 3063
Fragments arranged by size:
3063 2919 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2494 3642 4418 5802 6070 1632
Size: 862 1148 776 1384 268 1917
Fragments arranged by size:
1917 1384 1148 862 776 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6158
AflIII A'CryG_T
Cuts at: 1203 5254 6231 1203
Size: 4051 977 1327
Fragments arranged by size:
4051 1327 977
AgeI A'CCGG_T
Cuts at: 5186 5186
Size: 6355
AhdI GACnn_n'nnGTC
Cuts at: 5084 5084
Size: 6355
AleI CACnn'nnGTG
Cuts at: 1166 3365 1166
Size: 2199 4156
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6355
AlwNI CAG_nnn'CTG
Cuts at: 3620 4098 5670 3620
Size: 478 1572 4305
Fragments arranged by size:
4305 1572 478
ApaLI G'TGCA_C
Cuts at: 4412 5568 4412
Size: 1156 5199
ApoI r'AATT_y
Cuts at: 1493 2420 2665 2989 3664 3890 4042 6151 1493
Size: 927 245 324 675 226 152 2109 1697
Fragments arranged by size:
2109 1697 927 675 324 245 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4909 160
Size: 872 3877 1606
Fragments arranged by size:
3877 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6355
AvaI C'yCGr_G
Cuts at: 1870 1943 3170 3680 1870
Size: 73 1227 510 4545
Fragments arranged by size:
4545 1227 510 73
BamHI G'GATC_C
Cuts at: 2763 3685 4052 2763
Size: 922 367 5066
Fragments arranged by size:
5066 922 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2093 3676 5131 618
Size: 325 217 627 306 1583 1455 1842
Fragments arranged by size:
1842 1583 1455 627 325 306 217
BanII G_rGCy'C
Cuts at: 1391 2077 3171 3201 3674 1391
Size: 686 1094 30 473 4072
Fragments arranged by size:
4072 1094 686 473 30
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5384
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2505 2539 4546 4580 1903
Size: 34 568 34 2007 34 3678
Fragments arranged by size:
3678 2007 568 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4247 5457 4247
Size: 1210 5145
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6355
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3595 4966 136
Size: 107 122 71 631 605 1923 1371 1525
Fragments arranged by size:
1923 1525 1371 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6350 6350
Size: 6355
BlpI GC'TnA_GC
Cuts at: 3772 3772
Size: 6355
Bme1580I G_kGCm'C
Cuts at: 1790 4416 5572 1790
Size: 2626 1156 2573
Fragments arranged by size:
2626 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2032 1318
Size: 714 5641
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3174 4086 5044
Size: 416 53 83 399 24 1757 912 958
Cuts at: 5044 442
Size: 1753
Fragments arranged by size:
1757 1753 958 912 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6355
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 2654 2958 3514 5015 1239
Size: 917 498 304 556 1501 2579
Fragments arranged by size:
2579 1501 917 556 498 304
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4480 5345 5643 4480
Size: 865 298 5192
Fragments arranged by size:
5192 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2783 3035 3533 5018 882
Size: 617 1284 252 498 1485 2219
Fragments arranged by size:
2219 1485 1284 617 498 252
BsaAI yAC'GTr
Cuts at: 493 1204 4146 493
Size: 711 2942 2702
Fragments arranged by size:
2942 2702 711
BsaBI GATnn'nnATC
Cuts at: 6349 6349
Size: 6355
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2021 4162 4544 5179
Size: 53 83 186 936 488 2141 382 635
Cuts at: 5179 275
Size: 1451
Fragments arranged by size:
2141 1451 936 635 488 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4788 5186 5460 5607 6018 4788
Size: 398 274 147 411 5125
Fragments arranged by size:
5125 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2621 2651 1178
Size: 30 165 30 1218 30 4882
Fragments arranged by size:
4882 1218 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2123 3551 1186
Size: 937 1428 3990
Fragments arranged by size:
3990 1428 937
BseYI C'CCAG_C
Cuts at: 2014 2076 2853 2922 5558 6054 2014
Size: 62 777 69 2636 496 2315
Fragments arranged by size:
2636 2315 777 496 69 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5782
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3805 4566 5594 1540
Size: 275 186 1804 761 1028 2301
Fragments arranged by size:
2301 1804 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3201 3360 4416 4501 5572 3201
Size: 159 1056 85 1071 3984
Fragments arranged by size:
3984 1071 1056 159 85
BsmI GAATG_Cn'
Cuts at: 3866 3959 3866
Size: 93 6262
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2077 3171 3201 3360 3674 4416 4501
Size: 399 287 1094 30 159 314 742 85
Cuts at: 4501 5572 1391
Size: 1071 2174
Fragments arranged by size:
2174 1094 1071 742 399 314 287 159
85 30
BspEI T'CCGG_A
Cuts at: 6018 6018
Size: 6355
BspHI T'CATG_A
Cuts at: 4244 5974 4244
Size: 1730 4625
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2793 3710 844
Size: 1949 917 3489
Fragments arranged by size:
3489 1949 917
BsrBI CCG'CTC
Cuts at: 1394 1823 4242 1394
Size: 429 2419 3507
Fragments arranged by size:
3507 2419 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2521 3259 4850 5024 65
Size: 1183 1273 738 1591 174 1396
Fragments arranged by size:
1591 1396 1273 1183 738 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4999 5186 1700
Size: 110 3189 187 2869
Fragments arranged by size:
3189 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6355
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6355
BssSI C'ACGA_G
Cuts at: 2895 3533 4415 5427 2895
Size: 638 882 1012 3823
Fragments arranged by size:
3823 1012 882 638
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2876 794
Size: 2082 4273
BstBI TT'CG_AA
Cuts at: 5159 5159
Size: 6355
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6355
BstXI CCAn_nnnn'nTGG
Cuts at: 3601 3601
Size: 6355
BstYI r'GATC_y
Cuts at: 1972 2763 3180 3685 4052 4437 4454 5906 5992
Size: 791 417 505 367 385 17 1452 86
Cuts at: 5992 6004 6350 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 791 505 417 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 2448 2448
Size: 6355
BtgI C'CryG_G
Cuts at: 513 1065 2004 3306 513
Size: 552 939 1302 3562
Fragments arranged by size:
3562 1302 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5616
Fragments arranged by size:
5616 683 56
BtsI GCAGTG_nn'
Cuts at: 3871 4665 4685 3871
Size: 794 20 5541
Fragments arranged by size:
5541 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6355
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2667 2702 529
Size: 35 2103 35 4182
Fragments arranged by size:
4182 2103 35 35
DraI TTT'AAA
Cuts at: 3659 3659
Size: 6355
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2603 5362 809
Size: 507 1287 2759 1802
Fragments arranged by size:
2759 1802 1287 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2067 3087
Size: 54 1425 50 275 18 177 60 1020
Cuts at: 3087 3402 3802 4691 6320 8
Size: 315 400 889 1629 43
Fragments arranged by size:
1629 1425 1020 889 400 315 275 177
60 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3802 1537
Size: 275 1990 4090
Fragments arranged by size:
4090 1990 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3206 4285 1353
Size: 1853 1079 3423
Fragments arranged by size:
3423 1853 1079
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4942 5316 5462 387
Size: 1064 138 3353 374 146 1280
Fragments arranged by size:
3353 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3199 3199
Size: 6355
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2494 2654 2958 3514 3642 4418
Size: 393 524 338 160 304 556 128 776
Cuts at: 4418 5015 5802 6070 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 776 597 556 524 393 338
304 268 160 128
EcoRI G'AATT_C
Cuts at: 2420 3664 4042 2420
Size: 1244 378 4733
Fragments arranged by size:
4733 1244 378
EcoRV GAT'ATC
Cuts at: 2401 2401
Size: 6355
FspI TGC'GCA
Cuts at: 4861 4861
Size: 6355
HaeII r_GCGC'y
Cuts at: 1678 5502 1678
Size: 3824 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2621 2653 3274 3306 4996
Size: 32 135 32 1049 32 621 32 1690
Cuts at: 4996 5028 5070 5102 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1690 1049 621 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3699 3945 669
Size: 988 281 1761 246 3079
Fragments arranged by size:
3079 1761 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6355
HpaI GTT'AAC
Cuts at: 3945 3945
Size: 6355
KpnI G_GTAC'C
Cuts at: 3680 3680
Size: 6355
MfeI C'AATT_G
Cuts at: 3954 3954
Size: 6355
MluI A'CGCG_T
Cuts at: 6231 6231
Size: 6355
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2329 5469 5653 1305
Size: 1024 3140 184 2007
Fragments arranged by size:
3140 2007 1024 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2069 3089 10
Size: 54 1425 343 237 1020 3276
Fragments arranged by size:
3276 1425 1020 343 237 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2801 3328 3365 3413 4313 4672 4831
Size: 648 1635 527 37 48 900 359 159
Cuts at: 4831 518
Size: 2042
Fragments arranged by size:
2042 1635 900 648 527 359 159 48
37
MspA1I CmG'CkG
Cuts at: 1606 2058 2080 3771 4448 5596 5841 1606
Size: 452 22 1691 677 1148 245 2120
Fragments arranged by size:
2120 1691 1148 677 452 245 22
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4864
Fragments arranged by size:
4864 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6355
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6355
NotI GC'GGCC_GC
Cuts at: 3802 3802
Size: 6355
NspI r_CATG'y
Cuts at: 1132 1229 2548 3190 3713 5258 1132
Size: 97 1319 642 523 1545 2229
Fragments arranged by size:
2229 1545 1319 642 523 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6355
PciI A'CATG_T
Cuts at: 5254 5254
Size: 6355
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3036 1108
Size: 1928 4427
PfoI T'CCnGG_A
Cuts at: 2172 2172
Size: 6355
PmeI GTTT'AAAC
Cuts at: 3659 3659
Size: 6355
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4424 4456 5937 5969 4424
Size: 32 1481 32 4810
Fragments arranged by size:
4810 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3101 1613
Size: 1488 4867
PsiI TTA'TAA
Cuts at: 3925 3925
Size: 6355
PstI C_TGCA'G
Cuts at: 830 2394 3707 830
Size: 1564 1313 3478
Fragments arranged by size:
3478 1564 1313
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6355
PvuII CAG'CTG
Cuts at: 2058 2080 2058
Size: 22 6333
SacI G_AGCT'C
Cuts at: 3201 3201
Size: 6355
SalI G'TCGA_C
Cuts at: 1936 3697 1936
Size: 1761 4594
SapI GCTCTTCn'nnn_
Cuts at: 3206 3206
Size: 6355
SbfI CC_TGCA'GG
Cuts at: 3707 3707
Size: 6355
ScaI AGT'ACT
Cuts at: 4603 6315 4603
Size: 1712 4643
SfcI C'TryA_G
Cuts at: 826 1045 2103 2390 3703 4838 5519 5710 826
Size: 219 1058 287 1313 1135 681 191 1471
Fragments arranged by size:
1471 1313 1135 1058 681 287 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6355
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6355
SmaI CCC'GGG
Cuts at: 1872 3682 1872
Size: 1810 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4459 5360 5622 820
Size: 197 926 2516 901 262 1553
Fragments arranged by size:
2516 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4146 493
Size: 3653 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6355
SphI G_CATG'C
Cuts at: 1132 3190 3713 1132
Size: 2058 523 3774
Fragments arranged by size:
3774 2058 523
SspI AAT'ATT
Cuts at: 5 52 4279 5
Size: 47 4227 2081
Fragments arranged by size:
4227 2081 47
StuI AGG'CCT
Cuts at: 1517 3598 1517
Size: 2081 4274
StyI C'CwwG_G
Cuts at: 513 1065 2004 2478 3543 3631 3794 513
Size: 552 939 474 1065 88 163 3074
Fragments arranged by size:
3074 1065 939 552 474 163 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4382 4399 4552 4737 5182 325
Size: 1255 2802 17 153 185 445 1498
Fragments arranged by size:
2802 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2140 4601 6313
Size: 274 80 33 51 1443 163 2461 1712
Cuts at: 6313 96
Size: 138
Fragments arranged by size:
2461 1712 1443 274 163 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3513 3545 6315 6347 3513
Size: 32 2770 32 3521
Fragments arranged by size:
3521 2770 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6355
XbaI T'CTAG_A
Cuts at: 3691 3691
Size: 6355
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2077 2233 3097 3412 2011
Size: 66 156 864 315 4954
Fragments arranged by size:
4954 864 315 156 66
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6355
XmaI C'CCGG_G
Cuts at: 1870 3680 1870
Size: 1810 4545
XmnI GAAnn'nnTTC
Cuts at: 4484 4484
Size: 6355
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4163 276
Size: 53 83 186 936 2629 2468
Fragments arranged by size:
2629 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII
EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI