Restriction map for
pFN21AB5956
(Circular) (Six-base) MAPSORT of: pFN21AB5956.seq Check: 9572 from: 1 to: 6349
pFN21AB5956 6349 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4159 278
Size: 53 83 186 936 2623 2468
Fragments arranged by size:
2623 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3692 1937
Size: 1755 4594
Acc65I G'GTAC_C
Cuts at: 3670 3670
Size: 6349
AclI AA'CG_TT
Cuts at: 1563 4476 4849 1563
Size: 2913 373 3063
Fragments arranged by size:
3063 2913 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4412 5796 6064 1632
Size: 2780 1384 268 1917
Fragments arranged by size:
2780 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6152
AflIII A'CryG_T
Cuts at: 1203 5248 6225 1203
Size: 4045 977 1327
Fragments arranged by size:
4045 1327 977
AgeI A'CCGG_T
Cuts at: 5180 5180
Size: 6349
AhdI GACnn_n'nnGTC
Cuts at: 5078 5078
Size: 6349
AleI CACnn'nnGTG
Cuts at: 1166 2758 1166
Size: 1592 4757
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2591 1184
Size: 1407 4942
AlwNI CAG_nnn'CTG
Cuts at: 2455 4092 5664 2455
Size: 1637 1572 3140
Fragments arranged by size:
3140 1637 1572
ApaI G_GGCC'C
Cuts at: 2060 3100 2060
Size: 1040 5309
ApaLI G'TGCA_C
Cuts at: 4406 5562 4406
Size: 1156 5193
ApoI r'AATT_y
Cuts at: 1493 3658 3884 4036 6145 1493
Size: 2165 226 152 2109 1697
Fragments arranged by size:
2165 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4903 160
Size: 872 3871 1606
Fragments arranged by size:
3871 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6349
AvaI C'yCGr_G
Cuts at: 1870 1943 2769 2842 3674 1870
Size: 73 826 73 832 4545
Fragments arranged by size:
4545 832 826 73 73
BamHI G'GATC_C
Cuts at: 3679 4046 3679
Size: 367 5982
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2027 3005 3670 5125 618
Size: 325 217 627 240 978 665 1455 1842
Fragments arranged by size:
1842 1455 978 665 627 325 240 217
BanII G_rGCy'C
Cuts at: 1391 2060 3100 3645 3668 1391
Size: 669 1040 545 23 4072
Fragments arranged by size:
4072 1040 669 545 23
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5378
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2161 2195 4540 4574 1903
Size: 34 224 34 2345 34 3678
Fragments arranged by size:
3678 2345 224 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4241 5451 4241
Size: 1210 5139
BclI T'GATC_A
Cuts at: 1548 2598 1548
Size: 1050 5299
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2490 3032 4960
Size: 107 122 71 631 605 818 542 1928
Cuts at: 4960 136
Size: 1525
Fragments arranged by size:
1928 1525 818 631 605 542 122 107
71
BglII A'GATC_T
Cuts at: 6344 6344
Size: 6349
BlpI GC'TnA_GC
Cuts at: 3766 3766
Size: 6349
Bme1580I G_kGCm'C
Cuts at: 1790 2032 2060 2363 2643 3008 3100 4410 5566
Size: 242 28 303 280 365 92 1310 1156
Cuts at: 5566 1790
Size: 2573
Fragments arranged by size:
2573 1310 1156 365 303 280 242 92
28
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6349
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2013 2056 3094
Size: 416 53 83 399 24 596 43 1038
Cuts at: 3094 4080 5038 442
Size: 986 958 1753
Fragments arranged by size:
1753 1038 986 958 596 416 399 83
53 43 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6349
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2356 2561 2807 5009 1239
Size: 1117 205 246 2202 2579
Fragments arranged by size:
2579 2202 1117 246 205
Bpu10I CC'TnA_GC
Cuts at: 3169 3169
Size: 6349
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2261 4474 5339 5637 2261
Size: 2213 865 298 2973
Fragments arranged by size:
2973 2213 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3127 3548 5012 882
Size: 617 1628 421 1464 2219
Fragments arranged by size:
2219 1628 1464 617 421
BsaAI yAC'GTr
Cuts at: 493 1204 3262 4140 493
Size: 711 2058 878 2702
Fragments arranged by size:
2702 2058 878 711
BsaBI GATnn'nnATC
Cuts at: 2438 6343 2438
Size: 3905 2444
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4156 4538 5173 275
Size: 53 83 186 936 2623 382 635 1451
Fragments arranged by size:
2623 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4782 5180 5454 5601 6012 4782
Size: 398 274 147 411 5119
Fragments arranged by size:
5119 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2780 2810 1178
Size: 30 165 30 1377 30 4717
Fragments arranged by size:
4717 1377 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2276 3311 3371 3506 1186
Size: 1090 1035 60 135 4029
Fragments arranged by size:
4029 1090 1035 135 60
BseYI C'CCAG_C
Cuts at: 2032 5552 6048 2032
Size: 3520 496 2333
Fragments arranged by size:
3520 2333 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3398 3533 3620 1163
Size: 573 1662 135 87 3892
Fragments arranged by size:
3892 1662 573 135 87
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2367 2442 3799 4560 5588 1540
Size: 275 186 366 75 1357 761 1028 2301
Fragments arranged by size:
2301 1357 1028 761 366 275 186 75
BsiHKAI G_wGCw'C
Cuts at: 4410 4495 5566 4410
Size: 85 1071 5193
Fragments arranged by size:
5193 1071 85
BsmI GAATG_Cn'
Cuts at: 3860 3953 3860
Size: 93 6256
BsmBI CGTCTCn'nnnn_
Cuts at: 2374 2553 2374
Size: 179 6170
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2032 2060 2363 2643 3008 3100 3645
Size: 399 242 28 303 280 365 92 545
Cuts at: 3645 3668 4410 4495 5566 1391
Size: 23 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 545 399 365 303 280
242 92 85 28 23
BspEI T'CCGG_A
Cuts at: 6012 6012
Size: 6349
BspHI T'CATG_A
Cuts at: 4238 5968 4238
Size: 1730 4619
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3630 3704 844
Size: 2786 74 3489
Fragments arranged by size:
3489 2786 74
BsrBI CCG'CTC
Cuts at: 1394 1823 4236 1394
Size: 429 2413 3507
Fragments arranged by size:
3507 2413 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3430 4844 5018 65
Size: 1183 2182 1414 174 1396
Fragments arranged by size:
2182 1414 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4993 5180 1700
Size: 110 3183 187 2869
Fragments arranged by size:
3183 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6349
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6349
BssSI C'ACGA_G
Cuts at: 4409 5421 4409
Size: 1012 5337
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6349
BstBI TT'CG_AA
Cuts at: 5153 5153
Size: 6349
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6349
BstYI r'GATC_y
Cuts at: 1972 3679 4046 4431 4448 5900 5986 5998 6344
Size: 1707 367 385 17 1452 86 12 346
Cuts at: 6344 1972
Size: 1977
Fragments arranged by size:
1977 1707 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2052 3372 3441 513
Size: 552 939 48 1320 69 3421
Fragments arranged by size:
3421 1320 939 552 69 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5610
Fragments arranged by size:
5610 683 56
BtsI GCAGTG_nn'
Cuts at: 3865 4659 4679 3865
Size: 794 20 5535
Fragments arranged by size:
5535 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6349
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6314
DraI TTT'AAA
Cuts at: 3653 3653
Size: 6349
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2468 2749 3119 5356 809
Size: 507 1152 281 370 2237 1802
Fragments arranged by size:
2237 1802 1152 507 370 281
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2098 3024 3300
Size: 54 1425 50 275 18 268 926 276
Cuts at: 3300 3561 3796 4685 6314 8
Size: 261 235 889 1629 43
Fragments arranged by size:
1629 1425 926 889 276 275 268 261
235 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3796 1537
Size: 275 1984 4090
Fragments arranged by size:
4090 1984 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4279 1353
Size: 2926 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3593 4936 5310 5456 387
Size: 1064 138 2004 1343 374 146 1280
Fragments arranged by size:
2004 1343 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2356 2561 2807 4412 5009 5796 6064
Size: 393 724 205 246 1605 597 787 268
Cuts at: 6064 1239
Size: 1524
Fragments arranged by size:
1605 1524 787 724 597 393 268 246
205
EcoO109I rG'GnC_Cy
Cuts at: 2057 2512 3096 2057
Size: 455 584 5310
Fragments arranged by size:
5310 584 455
EcoRI G'AATT_C
Cuts at: 3658 4036 3658
Size: 378 5971
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2533 2533
Size: 6349
FspI TGC'GCA
Cuts at: 4855 4855
Size: 6349
HaeII r_GCGC'y
Cuts at: 1678 5496 1678
Size: 3818 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2111 2143 2763 2795 2963
Size: 32 135 32 539 32 620 32 168
Cuts at: 2963 2995 4990 5022 5064 5096 1373
Size: 32 1995 32 42 32 2626
Fragments arranged by size:
2626 1995 620 539 168 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3256 3693 3939 669
Size: 988 281 1318 437 246 3079
Fragments arranged by size:
3079 1318 988 437 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6349
HpaI GTT'AAC
Cuts at: 3939 3939
Size: 6349
KpnI G_GTAC'C
Cuts at: 3674 3674
Size: 6349
MfeI C'AATT_G
Cuts at: 3948 3948
Size: 6349
MluI A'CGCG_T
Cuts at: 6225 6225
Size: 6349
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2160 2484 2914 2979 5463 5647 1305
Size: 855 324 430 65 2484 184 2007
Fragments arranged by size:
2484 2007 855 430 324 184 65
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2100 3563 10
Size: 54 1425 343 268 1463 2796
Fragments arranged by size:
2796 1463 1425 343 268 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2758 3410 4307 4666 4825 518
Size: 648 1592 652 897 359 159 2042
Fragments arranged by size:
2042 1592 897 652 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2039 2785 3295 3765 4442 5590 5835 1606
Size: 433 746 510 470 677 1148 245 2120
Fragments arranged by size:
2120 1148 746 677 510 470 433 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2052 3441 513
Size: 552 939 48 1389 3421
Fragments arranged by size:
3421 1389 939 552 48
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6349
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6349
NotI GC'GGCC_GC
Cuts at: 3796 3796
Size: 6349
NspI r_CATG'y
Cuts at: 1132 1229 3371 3472 3707 5252 1132
Size: 97 2142 101 235 1545 2229
Fragments arranged by size:
2229 2142 1545 235 101 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6349
PciI A'CATG_T
Cuts at: 5248 5248
Size: 6349
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6349
PfoI T'CCnGG_A
Cuts at: 3450 3450
Size: 6349
PmeI GTTT'AAAC
Cuts at: 3653 3653
Size: 6349
PmlI CAC'GTG
Cuts at: 3262 3262
Size: 6349
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4418 4450 5931 5963 4418
Size: 32 1481 32 4804
Fragments arranged by size:
4804 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2512 2512
Size: 6349
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6349
PsiI TTA'TAA
Cuts at: 2550 3919 2550
Size: 1369 4980
PspOMI G'GGCC_C
Cuts at: 2056 3096 2056
Size: 1040 5309
PstI C_TGCA'G
Cuts at: 830 2354 3701 830
Size: 1524 1347 3478
Fragments arranged by size:
3478 1524 1347
PvuI CG_AT'CG
Cuts at: 2001 2367 2442 2001
Size: 366 75 5908
Fragments arranged by size:
5908 366 75
SalI G'TCGA_C
Cuts at: 1936 3691 1936
Size: 1755 4594
SbfI CC_TGCA'GG
Cuts at: 3701 3701
Size: 6349
ScaI AGT'ACT
Cuts at: 3311 4597 6309 3311
Size: 1286 1712 3351
Fragments arranged by size:
3351 1712 1286
SfcI C'TryA_G
Cuts at: 826 1045 2350 3697 4832 5513 5704 826
Size: 219 1305 1347 1135 681 191 1471
Fragments arranged by size:
1471 1347 1305 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6349
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6349
SmaI CCC'GGG
Cuts at: 1872 3676 1872
Size: 1804 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2276 2769 4453 5354 5616 820
Size: 197 926 333 493 1684 901 262 1553
Fragments arranged by size:
1684 1553 926 901 493 333 262 197
SnaBI TAC'GTA
Cuts at: 493 4140 493
Size: 3647 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6349
SphI G_CATG'C
Cuts at: 1132 3371 3472 3707 1132
Size: 2239 101 235 3774
Fragments arranged by size:
3774 2239 235 101
SspI AAT'ATT
Cuts at: 5 52 4273 5
Size: 47 4221 2081
Fragments arranged by size:
4221 2081 47
StuI AGG'CCT
Cuts at: 1517 2392 1517
Size: 875 5474
StyI C'CwwG_G
Cuts at: 513 1065 2004 2052 2643 3441 3558 3788 513
Size: 552 939 48 591 798 117 230 3074
Fragments arranged by size:
3074 939 798 591 552 230 117 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4376 4393 4546 4731 5176 325
Size: 1255 2796 17 153 185 445 1498
Fragments arranged by size:
2796 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2604 3309 4595
Size: 274 80 33 51 1443 627 705 1286
Cuts at: 4595 6307 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1286 705 627 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2606 2638 2780 2812 6309 6341 2606
Size: 32 142 32 3497 32 2614
Fragments arranged by size:
3497 2614 142 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2777 3338 1316
Size: 1461 561 4327
Fragments arranged by size:
4327 1461 561
XbaI T'CTAG_A
Cuts at: 3685 3685
Size: 6349
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2018 2314 3409 3448 3571 2018
Size: 296 1095 39 123 4796
Fragments arranged by size:
4796 1095 296 123 39
XhoI C'TCGA_G
Cuts at: 1943 2769 1943
Size: 826 5523
XmaI C'CCGG_G
Cuts at: 1870 3674 1870
Size: 1804 4545
XmnI GAAnn'nnTTC
Cuts at: 2630 4478 2630
Size: 1848 4501
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4157 276
Size: 53 83 186 936 2623 2468
Fragments arranged by size:
2623 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI