Restriction map for
pFN21AB5955
(Circular) (Six-base) MAPSORT of: pFN21AB5955.seq Check: 6095 from: 1 to: 6178
pFN21AB5955 6178 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3988 278
Size: 53 83 186 936 2452 2468
Fragments arranged by size:
2468 2452 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3521 1937
Size: 1584 4594
Acc65I G'GTAC_C
Cuts at: 3499 3499
Size: 6178
AclI AA'CG_TT
Cuts at: 1563 3407 4305 4678 1563
Size: 1844 898 373 3063
Fragments arranged by size:
3063 1844 898 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2792 2954 4241 5625 5893 1632
Size: 1160 162 1287 1384 268 1917
Fragments arranged by size:
1917 1384 1287 1160 268 162
AfeI AGC'GCT
Cuts at: 2389 2389
Size: 6178
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5981
AflIII A'CryG_T
Cuts at: 1203 5077 6054 1203
Size: 3874 977 1327
Fragments arranged by size:
3874 1327 977
AgeI A'CCGG_T
Cuts at: 5009 5009
Size: 6178
AhdI GACnn_n'nnGTC
Cuts at: 2185 4907 2185
Size: 2722 3456
AleI CACnn'nnGTG
Cuts at: 1166 3079 1166
Size: 1913 4265
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2975 1184
Size: 1791 4387
AlwNI CAG_nnn'CTG
Cuts at: 3921 5493 3921
Size: 1572 4606
ApaLI G'TGCA_C
Cuts at: 4235 5391 4235
Size: 1156 5022
ApoI r'AATT_y
Cuts at: 1493 3264 3487 3713 3865 5974 1493
Size: 1771 223 226 152 2109 1697
Fragments arranged by size:
2109 1771 1697 226 223 152
AseI AT'TA_AT
Cuts at: 160 1032 4732 160
Size: 872 3700 1606
Fragments arranged by size:
3700 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6178
AvaI C'yCGr_G
Cuts at: 1870 1943 2670 3503 1870
Size: 73 727 833 4545
Fragments arranged by size:
4545 833 727 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2731 2764 2731
Size: 33 6145
BamHI G'GATC_C
Cuts at: 3508 3875 3508
Size: 367 5811
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2374 3499 4954 618
Size: 325 217 627 587 1125 1455 1842
Fragments arranged by size:
1842 1455 1125 627 587 325 217
BanII G_rGCy'C
Cuts at: 1391 2883 3357 3497 1391
Size: 1492 474 140 4072
Fragments arranged by size:
4072 1492 474 140
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2069 2831 928
Size: 971 170 762 4275
Fragments arranged by size:
4275 971 762 170
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4369 4403 1903
Size: 34 2432 34 3678
Fragments arranged by size:
3678 2432 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4070 5280 4070
Size: 1210 4968
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6178
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4789 136
Size: 107 122 71 631 605 3117 1525
Fragments arranged by size:
3117 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6173 6173
Size: 6178
BlpI GC'TnA_GC
Cuts at: 3595 3595
Size: 6178
Bme1580I G_kGCm'C
Cuts at: 1790 4239 5395 1790
Size: 2449 1156 2573
Fragments arranged by size:
2573 2449 1156
BmgBI CAC'GTC
Cuts at: 1318 3295 1318
Size: 1977 4201
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3909 4867 442
Size: 416 53 83 399 24 2492 958 1753
Fragments arranged by size:
2492 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6178
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2240 2412 3159 4838 1239
Size: 1001 172 747 1679 2579
Fragments arranged by size:
2579 1679 1001 747 172
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2059 4303 5168 5466 2059
Size: 2244 865 298 2771
Fragments arranged by size:
2771 2244 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3070 3394 4841 882
Size: 617 1571 324 1447 2219
Fragments arranged by size:
2219 1571 1447 617 324
BsaAI yAC'GTr
Cuts at: 493 1204 3969 493
Size: 711 2765 2702
Fragments arranged by size:
2765 2702 711
BsaBI GATnn'nnATC
Cuts at: 6172 6172
Size: 6178
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3985 4367 5002 275
Size: 53 83 186 936 2452 382 635 1451
Fragments arranged by size:
2452 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4611 5009 5283 5430 5841 4611
Size: 398 274 147 411 4948
Fragments arranged by size:
4948 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2127 2157 1178
Size: 30 165 30 724 30 5199
Fragments arranged by size:
5199 724 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2237 2891 1186
Size: 1051 654 4473
Fragments arranged by size:
4473 1051 654
BseYI C'CCAG_C
Cuts at: 2282 2705 5381 5877 2282
Size: 423 2676 496 2583
Fragments arranged by size:
2676 2583 496 423
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2617 1163
Size: 573 881 4724
Fragments arranged by size:
4724 881 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3628 4389 5417 1540
Size: 275 186 1627 761 1028 2301
Fragments arranged by size:
2301 1627 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3357 4239 4324 5395 3357
Size: 882 85 1071 4140
Fragments arranged by size:
4140 1071 882 85
BsmI GAATG_Cn'
Cuts at: 3689 3782 3689
Size: 93 6085
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2883 3357 3497 4239 4324 5395 1391
Size: 399 1093 474 140 742 85 1071 2174
Fragments arranged by size:
2174 1093 1071 742 474 399 140 85
BspEI T'CCGG_A
Cuts at: 5841 5841
Size: 6178
BspHI T'CATG_A
Cuts at: 2820 4067 5797 2820
Size: 1247 1730 3201
Fragments arranged by size:
3201 1730 1247
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3051 3533 844
Size: 2207 482 3489
Fragments arranged by size:
3489 2207 482
BsrBI CCG'CTC
Cuts at: 1394 1823 3019 4065 1394
Size: 429 1196 1046 3507
Fragments arranged by size:
3507 1196 1046 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4673 4847 65
Size: 1183 3425 174 1396
Fragments arranged by size:
3425 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4822 5009 1700
Size: 110 3012 187 2869
Fragments arranged by size:
3012 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6178
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6178
BssSI C'ACGA_G
Cuts at: 2846 4238 5250 2846
Size: 1392 1012 3774
Fragments arranged by size:
3774 1392 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6178
BstBI TT'CG_AA
Cuts at: 4982 4982
Size: 6178
BstEII G'GTnAC_C
Cuts at: 1184 2866 1184
Size: 1682 4496
BstXI CCAn_nnnn'nTGG
Cuts at: 2686 2686
Size: 6178
BstYI r'GATC_y
Cuts at: 1972 2119 2664 3508 3875 4260 4277 5729 5815
Size: 147 545 844 367 385 17 1452 86
Cuts at: 5815 5827 6173 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 844 545 385 367 346 147
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2642 3450 513
Size: 552 939 638 808 3241
Fragments arranged by size:
3241 939 808 638 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2955 487
Size: 683 56 1729 3710
Fragments arranged by size:
3710 1729 683 56
BtsI GCAGTG_nn'
Cuts at: 3694 4488 4508 3694
Size: 794 20 5364
Fragments arranged by size:
5364 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6178
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6143
DraI TTT'AAA
Cuts at: 3482 3482
Size: 6178
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3307 5185 809
Size: 507 1991 1878 1802
Fragments arranged by size:
1991 1878 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2295 2907 3625
Size: 54 1425 50 275 18 465 612 718
Cuts at: 3625 4514 6143 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 718 612 465 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3625 1537
Size: 275 1813 4090
Fragments arranged by size:
4090 1813 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2316 2930 3390 4108 1353
Size: 963 614 460 718 3423
Fragments arranged by size:
3423 963 718 614 460
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4765 5139 5285 387
Size: 1064 138 3176 374 146 1280
Fragments arranged by size:
3176 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3355 3355
Size: 6178
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2240 2412 2792 2954 3159 4241 4838
Size: 393 608 172 380 162 205 1082 597
Cuts at: 4838 5625 5893 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1082 787 608 597 393 380 268
205 172 162
EcoNI CCTnn'n_nnAGG
Cuts at: 3311 3311
Size: 6178
EcoO109I rG'GnC_Cy
Cuts at: 3040 3040
Size: 6178
EcoRI G'AATT_C
Cuts at: 3487 3865 3487
Size: 378 5800
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2978 2978
Size: 6178
FspI TGC'GCA
Cuts at: 4684 4684
Size: 6178
HaeII r_GCGC'y
Cuts at: 1678 2391 2706 5325 1678
Size: 713 315 2619 2531
Fragments arranged by size:
2619 2531 713 315
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2171 2203 3206 3238 4819
Size: 32 135 32 599 32 1003 32 1581
Cuts at: 4819 4851 4893 4925 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1581 1003 599 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3522 3768 669
Size: 988 281 1584 246 3079
Fragments arranged by size:
3079 1584 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2431 748
Size: 1683 4495
HpaI GTT'AAC
Cuts at: 3768 3768
Size: 6178
KpnI G_GTAC'C
Cuts at: 3503 3503
Size: 6178
MfeI C'AATT_G
Cuts at: 3777 3777
Size: 6178
MluI A'CGCG_T
Cuts at: 6054 6054
Size: 6178
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5292 5476 1305
Size: 3987 184 2007
Fragments arranged by size:
3987 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2297 2909 10
Size: 54 1425 343 465 612 3279
Fragments arranged by size:
3279 1425 612 465 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2472 3079 4136 4495 4654 518
Size: 648 1306 607 1057 359 159 2042
Fragments arranged by size:
2042 1306 1057 648 607 359 159
MspA1I CmG'CkG
Cuts at: 1606 3594 4271 5419 5664 1606
Size: 1988 677 1148 245 2120
Fragments arranged by size:
2120 1988 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2642 513
Size: 552 939 638 4049
Fragments arranged by size:
4049 939 638 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6178
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6178
NotI GC'GGCC_GC
Cuts at: 3625 3625
Size: 6178
NspI r_CATG'y
Cuts at: 1132 1229 3536 5081 1132
Size: 97 2307 1545 2229
Fragments arranged by size:
2307 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3275 3313 1348
Size: 1927 38 4213
Fragments arranged by size:
4213 1927 38
PciI A'CATG_T
Cuts at: 5077 5077
Size: 6178
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6178
PfoI T'CCnGG_A
Cuts at: 2217 2802 3429 2217
Size: 585 627 4966
Fragments arranged by size:
4966 627 585
PmeI GTTT'AAAC
Cuts at: 3482 3482
Size: 6178
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4247 4279 5760 5792 4247
Size: 32 1481 32 4633
Fragments arranged by size:
4633 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3040 3040
Size: 6178
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6178
PsiI TTA'TAA
Cuts at: 3748 3748
Size: 6178
PstI C_TGCA'G
Cuts at: 830 2835 3530 830
Size: 2005 695 3478
Fragments arranged by size:
3478 2005 695
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6178
SacI G_AGCT'C
Cuts at: 3357 3357
Size: 6178
SalI G'TCGA_C
Cuts at: 1936 3520 1936
Size: 1584 4594
SapI GCTCTTCn'nnn_
Cuts at: 2930 2930
Size: 6178
SbfI CC_TGCA'GG
Cuts at: 3530 3530
Size: 6178
ScaI AGT'ACT
Cuts at: 4426 6138 4426
Size: 1712 4466
SfcI C'TryA_G
Cuts at: 826 1045 2831 3319 3526 4661 5342 5533 826
Size: 219 1786 488 207 1135 681 191 1471
Fragments arranged by size:
1786 1471 1135 681 488 219 207 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6178
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6178
SmaI CCC'GGG
Cuts at: 1872 2672 3505 1872
Size: 800 833 4545
Fragments arranged by size:
4545 833 800
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2038 4282 5183 5445 820
Size: 197 926 95 2244 901 262 1553
Fragments arranged by size:
2244 1553 926 901 262 197 95
SnaBI TAC'GTA
Cuts at: 493 3969 493
Size: 3476 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6178
SphI G_CATG'C
Cuts at: 1132 3536 1132
Size: 2404 3774
SspI AAT'ATT
Cuts at: 5 52 4102 5
Size: 47 4050 2081
Fragments arranged by size:
4050 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6178
StyI C'CwwG_G
Cuts at: 513 1065 2004 2642 2751 2910 3617 513
Size: 552 939 638 109 159 707 3074
Fragments arranged by size:
3074 939 707 638 552 159 109
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4205 4222 4375 4560 5005 325
Size: 1255 2625 17 153 185 445 1498
Fragments arranged by size:
2625 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2901 3129 4424
Size: 274 80 33 51 1443 924 228 1295
Cuts at: 4424 6136 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1295 924 274 228 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2127 2159 3316 3348 6138 6170 2127
Size: 32 1157 32 2790 32 2135
Fragments arranged by size:
2790 2135 1157 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6178
XbaI T'CTAG_A
Cuts at: 3514 3514
Size: 6178
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2290 2640 2290
Size: 350 5828
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6178
XmaI C'CCGG_G
Cuts at: 1870 2670 3503 1870
Size: 800 833 4545
Fragments arranged by size:
4545 833 800
XmnI GAAnn'nnTTC
Cuts at: 4307 4307
Size: 6178
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3986 276
Size: 53 83 186 936 2452 2468
Fragments arranged by size:
2468 2452 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI