Restriction map for
pFN21AB5954
(Circular) (Six-base) MAPSORT of: pFN21AB5954.seq Check: 6084 from: 1 to: 6349
pFN21AB5954 6349 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3118 3118
Size: 6349
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4159 278
Size: 53 83 186 936 2623 2468
Fragments arranged by size:
2623 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3390 3509 3692 1937
Size: 1453 119 183 4594
Fragments arranged by size:
4594 1453 183 119
Acc65I G'GTAC_C
Cuts at: 3670 3670
Size: 6349
AclI AA'CG_TT
Cuts at: 1563 4476 4849 1563
Size: 2913 373 3063
Fragments arranged by size:
3063 2913 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2456 4412 5796 6064 1632
Size: 824 1956 1384 268 1917
Fragments arranged by size:
1956 1917 1384 824 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6152
AflIII A'CryG_T
Cuts at: 1203 2727 3393 3533 3609 5248 6225 1203
Size: 1524 666 140 76 1639 977 1327
Fragments arranged by size:
1639 1524 1327 977 666 140 76
AgeI A'CCGG_T
Cuts at: 3060 5180 3060
Size: 2120 4229
AhdI GACnn_n'nnGTC
Cuts at: 5078 5078
Size: 6349
AleI CACnn'nnGTG
Cuts at: 1166 3014 1166
Size: 1848 4501
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6349
AlwNI CAG_nnn'CTG
Cuts at: 4092 5664 4092
Size: 1572 4777
ApaLI G'TGCA_C
Cuts at: 4406 5562 4406
Size: 1156 5193
ApoI r'AATT_y
Cuts at: 1493 2431 3245 3658 3884 4036 6145 1493
Size: 938 814 413 226 152 2109 1697
Fragments arranged by size:
2109 1697 938 814 413 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3297 4903 160
Size: 872 2265 1606 1606
Fragments arranged by size:
2265 1606 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6349
AvaI C'yCGr_G
Cuts at: 1870 1943 2241 3674 1870
Size: 73 298 1433 4545
Fragments arranged by size:
4545 1433 298 73
BamHI G'GATC_C
Cuts at: 3679 4046 3679
Size: 367 5982
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2846 2994 3670 5125 618
Size: 325 217 627 1059 148 676 1455 1842
Fragments arranged by size:
1842 1455 1059 676 627 325 217 148
BanII G_rGCy'C
Cuts at: 1391 3135 3668 1391
Size: 1744 533 4072
Fragments arranged by size:
4072 1744 533
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5378
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3594 3628 4540 4574 1903
Size: 34 1657 34 912 34 3678
Fragments arranged by size:
3678 1657 912 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4241 5451 4241
Size: 1210 5139
BclI T'GATC_A
Cuts at: 1548 2661 1548
Size: 1113 5236
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4960 136
Size: 107 122 71 631 605 3288 1525
Fragments arranged by size:
3288 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6344 6344
Size: 6349
BlpI GC'TnA_GC
Cuts at: 3766 3766
Size: 6349
Bme1580I G_kGCm'C
Cuts at: 1790 4410 5566 1790
Size: 2620 1156 2573
Fragments arranged by size:
2620 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 3394 3610 1318
Size: 2076 216 4057
Fragments arranged by size:
4057 2076 216
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4080 5038 442
Size: 416 53 83 399 24 2663 958 1753
Fragments arranged by size:
2663 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2015 1056
Size: 959 5390
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2571 2664 5009 1239
Size: 1332 93 2345 2579
Fragments arranged by size:
2579 2345 1332 93
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4474 5339 5637 4474
Size: 865 298 5186
Fragments arranged by size:
5186 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2912 5012 882
Size: 617 1413 2100 2219
Fragments arranged by size:
2219 2100 1413 617
BsaAI yAC'GTr
Cuts at: 493 1204 2140 3016 4140 493
Size: 711 936 876 1124 2702
Fragments arranged by size:
2702 1124 936 876 711
BsaBI GATnn'nnATC
Cuts at: 6343 6343
Size: 6349
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4156 4538 5173 275
Size: 53 83 186 936 2623 382 635 1451
Fragments arranged by size:
2623 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3060 4782 5180 5454 5601 6012 3060
Size: 1722 398 274 147 411 3397
Fragments arranged by size:
3397 1722 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3323 3353 1178
Size: 30 165 30 1920 30 4174
Fragments arranged by size:
4174 1920 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2661 2684 2733 2802 1186
Size: 1475 23 49 69 4733
Fragments arranged by size:
4733 1475 69 49 23
BseYI C'CCAG_C
Cuts at: 2400 2929 5552 6048 2400
Size: 529 2623 496 2701
Fragments arranged by size:
2701 2623 529 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5776
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3799 4560 5588 1540
Size: 275 186 1798 761 1028 2301
Fragments arranged by size:
2301 1798 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3135 4410 4495 5566 3135
Size: 1275 85 1071 3918
Fragments arranged by size:
3918 1275 1071 85
BsmI GAATG_Cn'
Cuts at: 2559 3860 3953 2559
Size: 1301 93 4955
Fragments arranged by size:
4955 1301 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2032 3600 2032
Size: 1568 4781
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3135 3668 4410 4495 5566 1391
Size: 399 1345 533 742 85 1071 2174
Fragments arranged by size:
2174 1345 1071 742 533 399 85
BspEI T'CCGG_A
Cuts at: 6012 6012
Size: 6349
BspHI T'CATG_A
Cuts at: 4238 5968 4238
Size: 1730 4619
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2930 3118 3455 3704 844
Size: 2086 188 337 249 3489
Fragments arranged by size:
3489 2086 337 249 188
BsrBI CCG'CTC
Cuts at: 1394 1823 4236 1394
Size: 429 2413 3507
Fragments arranged by size:
3507 2413 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4844 5018 65
Size: 1183 3596 174 1396
Fragments arranged by size:
3596 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3060 4993 5180 1700
Size: 110 1250 1933 187 2869
Fragments arranged by size:
2869 1933 1250 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6349
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6349
BssSI C'ACGA_G
Cuts at: 4409 5421 4409
Size: 1012 5337
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6349
BstBI TT'CG_AA
Cuts at: 5153 5153
Size: 6349
BstEII G'GTnAC_C
Cuts at: 1184 2758 1184
Size: 1574 4775
BstYI r'GATC_y
Cuts at: 1972 3679 4046 4431 4448 5900 5986 5998 6344
Size: 1707 367 385 17 1452 86 12 346
Cuts at: 6344 1972
Size: 1977
Fragments arranged by size:
1977 1707 1452 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 3510 3510
Size: 6349
Bsu36I CC'TnA_GG
Cuts at: 3363 3363
Size: 6349
BtgI C'CryG_G
Cuts at: 513 1065 2004 2052 2526 2754 3541 513
Size: 552 939 48 474 228 787 3321
Fragments arranged by size:
3321 939 787 552 474 228 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2061 3370 487
Size: 683 56 835 1309 3466
Fragments arranged by size:
3466 1309 835 683 56
BtsI GCAGTG_nn'
Cuts at: 2542 2550 3865 4659 4679 2542
Size: 8 1315 794 20 4212
Fragments arranged by size:
4212 1315 794 20 8
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6349
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6314
DraI TTT'AAA
Cuts at: 2083 3653 2083
Size: 1570 4779
DraIII CAC_nnn'GTG
Cuts at: 2766 2766
Size: 6349
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2684 5356 809
Size: 507 1368 2672 1802
Fragments arranged by size:
2672 1802 1368 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2593 3796 4685
Size: 54 1425 50 275 18 763 1203 889
Cuts at: 4685 6314 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1203 889 763 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3796 1537
Size: 275 1984 4090
Fragments arranged by size:
4090 1984 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2225 2269 2861 2874 4279 1353
Size: 872 44 592 13 1405 3423
Fragments arranged by size:
3423 1405 872 592 44 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2501 4936 5310 5456 387
Size: 1064 138 912 2435 374 146 1280
Fragments arranged by size:
2435 1280 1064 912 374 146 138
EcoICRI GAG'CTC
Cuts at: 3133 3133
Size: 6349
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2456 2571 2664 4412 5009 5796 6064
Size: 393 824 115 93 1748 597 787 268
Cuts at: 6064 1239
Size: 1524
Fragments arranged by size:
1748 1524 824 787 597 393 268 115
93
EcoNI CCTnn'n_nnAGG
Cuts at: 2643 2643
Size: 6349
EcoRI G'AATT_C
Cuts at: 3658 4036 3658
Size: 378 5971
EcoRV GAT'ATC
Cuts at: 3259 3259
Size: 6349
FspI TGC'GCA
Cuts at: 2417 4855 2417
Size: 2438 3911
HaeII r_GCGC'y
Cuts at: 1678 5496 1678
Size: 3818 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2742 2774 3243 3275 3323
Size: 32 135 32 1170 32 469 32 48
Cuts at: 3323 3355 4990 5022 5064 5096 1373
Size: 32 1635 32 42 32 2626
Fragments arranged by size:
2626 1635 1170 469 135 48 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3391 3693 3939 669
Size: 988 281 1453 302 246 3079
Fragments arranged by size:
3079 1453 988 302 281 246
HindIII A'AGCT_T
Cuts at: 748 2861 748
Size: 2113 4236
HpaI GTT'AAC
Cuts at: 3939 3939
Size: 6349
KpnI G_GTAC'C
Cuts at: 3674 3674
Size: 6349
MfeI C'AATT_G
Cuts at: 3948 3948
Size: 6349
MluI A'CGCG_T
Cuts at: 6225 6225
Size: 6349
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2840 3045 5463 5647 1305
Size: 1535 205 2418 184 2007
Fragments arranged by size:
2418 2007 1535 205 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2595 10
Size: 54 1425 343 763 3764
Fragments arranged by size:
3764 1425 763 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3014 4307 4666 4825 518
Size: 648 1848 1293 359 159 2042
Fragments arranged by size:
2042 1848 1293 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3765 4442 5590 5835 1606
Size: 2159 677 1148 245 2120
Fragments arranged by size:
2159 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2754 3541 513
Size: 552 939 750 787 3321
Fragments arranged by size:
3321 939 787 750 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6349
NheI G'CTAG_C
Cuts at: 1052 2011 1052
Size: 959 5390
NotI GC'GGCC_GC
Cuts at: 3796 3796
Size: 6349
NspI r_CATG'y
Cuts at: 1132 1229 2731 3352 3537 3707 5252 1132
Size: 97 1502 621 185 170 1545 2229
Fragments arranged by size:
2229 1545 1502 621 185 170 97
PasI CC'CwG_GG
Cuts at: 1348 2935 1348
Size: 1587 4762
PciI A'CATG_T
Cuts at: 2727 3533 5248 2727
Size: 806 1715 3828
Fragments arranged by size:
3828 1715 806
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6349
PmeI GTTT'AAAC
Cuts at: 3653 3653
Size: 6349
PmlI CAC'GTG
Cuts at: 3016 3016
Size: 6349
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4418 4450 5931 5963 4418
Size: 32 1481 32 4804
Fragments arranged by size:
4804 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6349
PsiI TTA'TAA
Cuts at: 3471 3919 3471
Size: 448 5901
PstI C_TGCA'G
Cuts at: 830 3115 3701 830
Size: 2285 586 3478
Fragments arranged by size:
3478 2285 586
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6349
SacI G_AGCT'C
Cuts at: 3135 3135
Size: 6349
SalI G'TCGA_C
Cuts at: 1936 3389 3691 1936
Size: 1453 302 4594
Fragments arranged by size:
4594 1453 302
SbfI CC_TGCA'GG
Cuts at: 3115 3701 3115
Size: 586 5763
ScaI AGT'ACT
Cuts at: 4597 6309 4597
Size: 1712 4637
SexAI A'CCwGG_T
Cuts at: 2762 2762
Size: 6349
SfcI C'TryA_G
Cuts at: 826 1045 3111 3697 4832 5513 5704 826
Size: 219 2066 586 1135 681 191 1471
Fragments arranged by size:
2066 1471 1135 681 586 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6349
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6349
SmaI CCC'GGG
Cuts at: 1872 2243 3676 1872
Size: 371 1433 4545
Fragments arranged by size:
4545 1433 371
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4453 5354 5616 820
Size: 197 926 2510 901 262 1553
Fragments arranged by size:
2510 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2140 4140 493
Size: 1647 2000 2702
Fragments arranged by size:
2702 2000 1647
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6349
SphI G_CATG'C
Cuts at: 1132 3707 1132
Size: 2575 3774
SspI AAT'ATT
Cuts at: 5 52 3099 4273 5
Size: 47 3047 1174 2081
Fragments arranged by size:
3047 2081 1174 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6349
StyI C'CwwG_G
Cuts at: 513 1065 2004 2067 2754 3541 3788 513
Size: 552 939 63 687 787 247 3074
Fragments arranged by size:
3074 939 787 687 552 247 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2049 4376 4393 4546 4731 5176 325
Size: 1255 469 2327 17 153 185 445 1498
Fragments arranged by size:
2327 1498 1255 469 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2910 3006 4595
Size: 274 80 33 51 1443 933 96 1589
Cuts at: 4595 6307 96
Size: 1712 138
Fragments arranged by size:
1712 1589 1443 933 274 138 96 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2480 2512 3011 3043 6309 6341 2480
Size: 32 499 32 3266 32 2488
Fragments arranged by size:
3266 2488 499 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6349
XbaI T'CTAG_A
Cuts at: 3685 3685
Size: 6349
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3548 3548
Size: 6349
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6349
XmaI C'CCGG_G
Cuts at: 1870 2241 3674 1870
Size: 371 1433 4545
Fragments arranged by size:
4545 1433 371
XmnI GAAnn'nnTTC
Cuts at: 2124 4478 2124
Size: 2354 3995
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4157 276
Size: 53 83 186 936 2623 2468
Fragments arranged by size:
2623 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PstI PvuI SacI SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI EcoO109I FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII
SanDI SapI SfiI SfoI SrfI SwaI