Restriction map for
pFN21AB5952
(Circular) (Six-base) MAPSORT of: pFN21AB5952.seq Check: 7674 from: 1 to: 6346
pFN21AB5952 6346 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2207 4156 278
Size: 53 83 186 936 671 1949 2468
Fragments arranged by size:
2468 1949 936 671 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2766 3689 1937
Size: 829 923 4594
Fragments arranged by size:
4594 923 829
Acc65I G'GTAC_C
Cuts at: 2017 3667 2017
Size: 1650 4696
AclI AA'CG_TT
Cuts at: 1563 4473 4846 1563
Size: 2910 373 3063
Fragments arranged by size:
3063 2910 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3068 3517 4409 5793 6061 1632
Size: 1436 449 892 1384 268 1917
Fragments arranged by size:
1917 1436 1384 892 449 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6149
AflIII A'CryG_T
Cuts at: 1203 3259 5245 6222 1203
Size: 2056 1986 977 1327
Fragments arranged by size:
2056 1986 1327 977
AgeI A'CCGG_T
Cuts at: 5177 5177
Size: 6346
AhdI GACnn_n'nnGTC
Cuts at: 5075 5075
Size: 6346
AleI CACnn'nnGTG
Cuts at: 1166 2459 1166
Size: 1293 5053
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3602 1184
Size: 2418 3928
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3140 3172 3140
Size: 32 6314
AlwNI CAG_nnn'CTG
Cuts at: 2043 2605 2791 4089 5661 2043
Size: 562 186 1298 1572 2728
Fragments arranged by size:
2728 1572 1298 562 186
ApaLI G'TGCA_C
Cuts at: 4403 5559 4403
Size: 1156 5190
ApoI r'AATT_y
Cuts at: 1493 3655 3881 4033 6142 1493
Size: 2162 226 152 2109 1697
Fragments arranged by size:
2162 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4900 160
Size: 872 3868 1606
Fragments arranged by size:
3868 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6346
AvaI C'yCGr_G
Cuts at: 1870 1943 2871 2919 3024 3064 3140 3296 3671
Size: 73 928 48 105 40 76 156 375
Cuts at: 3671 1870
Size: 4545
Fragments arranged by size:
4545 928 375 156 105 76 73 48
40
AvrII C'CTAG_G
Cuts at: 2896 2947 2896
Size: 51 6295
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2001 2034 2001
Size: 33 6313
BamHI G'GATC_C
Cuts at: 2283 3345 3676 4043 2283
Size: 1062 331 367 4586
Fragments arranged by size:
4586 1062 367 331
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2017 2371 2867 3334 3667
Size: 325 217 627 230 354 496 467 333
Cuts at: 3667 5122 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 496 467 354 333 325
230 217
BanII G_rGCy'C
Cuts at: 1391 2118 2563 2976 3025 3455 3665 1391
Size: 727 445 413 49 430 210 4072
Fragments arranged by size:
4072 727 445 430 413 210 49
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3463 3523 928
Size: 971 1564 60 3751
Fragments arranged by size:
3751 1564 971 60
BbvCI CC'TCA_GC
Cuts at: 2626 2966 3642 2626
Size: 340 676 5330
Fragments arranged by size:
5330 676 340
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3204 3238 4537 4571 1903
Size: 34 1267 34 1299 34 3678
Fragments arranged by size:
3678 1299 1267 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4238 5448 4238
Size: 1210 5136
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6346
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2386 2602 2756
Size: 107 122 71 631 605 714 216 154
Cuts at: 2756 4957 136
Size: 2201 1525
Fragments arranged by size:
2201 1525 714 631 605 216 154 122
107 71
BglII A'GATC_T
Cuts at: 6341 6341
Size: 6346
BlpI GC'TnA_GC
Cuts at: 2486 3763 2486
Size: 1277 5069
Bme1580I G_kGCm'C
Cuts at: 1790 2872 4407 5563 1790
Size: 1082 1535 1156 2573
Fragments arranged by size:
2573 1535 1156 1082
BmgBI CAC'GTC
Cuts at: 1318 3262 3597 1318
Size: 1944 335 4067
Fragments arranged by size:
4067 1944 335
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4077 5035 442
Size: 416 53 83 399 24 2660 958 1753
Fragments arranged by size:
2660 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6346
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2160 3307 5006 1239
Size: 921 1147 1699 2579
Fragments arranged by size:
2579 1699 1147 921
Bpu10I CC'TnA_GC
Cuts at: 2626 2966 3642 2626
Size: 340 676 5330
Fragments arranged by size:
5330 676 340
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4471 5336 5634 4471
Size: 865 298 5183
Fragments arranged by size:
5183 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5009 882
Size: 617 3510 2219
Fragments arranged by size:
3510 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4137 493
Size: 711 2933 2702
Fragments arranged by size:
2933 2702 711
BsaBI GATnn'nnATC
Cuts at: 6340 6340
Size: 6346
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2204 4153 4535 5170
Size: 53 83 186 936 671 1949 382 635
Cuts at: 5170 275
Size: 1451
Fragments arranged by size:
1949 1451 936 671 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4779 5177 5451 5598 6009 4779
Size: 398 274 147 411 5116
Fragments arranged by size:
5116 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2420 2450 3541 3571 1178
Size: 30 165 30 1017 30 1091 30 3953
Fragments arranged by size:
3953 1091 1017 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2317 2492 2495 2522 3587 1186
Size: 1131 175 3 27 1065 3945
Fragments arranged by size:
3945 1131 1065 175 27 3
BseYI C'CCAG_C
Cuts at: 2177 2241 2472 2605 2776 3017 3228 3446 5549
Size: 64 231 133 171 241 211 218 2103
Cuts at: 5549 6045 2177
Size: 496 2478
Fragments arranged by size:
2478 2103 496 241 231 218 211 171
133 64
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2710 1163
Size: 573 974 4799
Fragments arranged by size:
4799 974 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3057 3796 4557 5585 1540
Size: 275 186 1056 739 761 1028 2301
Fragments arranged by size:
2301 1056 1028 761 739 275 186
BsiHKAI G_wGCw'C
Cuts at: 2133 3455 3543 3581 4407 4492 5563 2133
Size: 1322 88 38 826 85 1071 2916
Fragments arranged by size:
2916 1322 1071 826 88 85 38
BsmI GAATG_Cn'
Cuts at: 2800 3857 3950 2800
Size: 1057 93 5196
Fragments arranged by size:
5196 1057 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2195 2541 2902 2195
Size: 346 361 5639
Fragments arranged by size:
5639 361 346
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2118 2133 2563 2872 2976 3025 3455
Size: 399 328 15 430 309 104 49 430
Cuts at: 3455 3543 3581 3665 4407 4492 5563 1391
Size: 88 38 84 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 430 430 399 328 309
104 88 85 84 49 38 15
BspEI T'CCGG_A
Cuts at: 6009 6009
Size: 6346
BspHI T'CATG_A
Cuts at: 2805 4235 5965 2805
Size: 1430 1730 3186
Fragments arranged by size:
3186 1730 1430
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3701 844
Size: 2857 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3552 4233 1394
Size: 429 1729 681 3507
Fragments arranged by size:
3507 1729 681 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2311 3624 4841 5015 65
Size: 1183 1063 1313 1217 174 1396
Fragments arranged by size:
1396 1313 1217 1183 1063 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2392 2490 2716 3337 4990 5177 1700
Size: 110 582 98 226 621 1653 187 2869
Fragments arranged by size:
2869 1653 621 582 226 187 110 98
BsrGI T'GTAC_A
Cuts at: 96 2343 96
Size: 2247 4099
BssHII G'CGCG_C
Cuts at: 1925 2849 2851 1925
Size: 924 2 5420
Fragments arranged by size:
5420 924 2
BssSI C'ACGA_G
Cuts at: 2678 3405 4406 5418 2678
Size: 727 1001 1012 3606
Fragments arranged by size:
3606 1012 1001 727
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6346
BstBI TT'CG_AA
Cuts at: 5150 5150
Size: 6346
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6346
BstXI CCAn_nnnn'nTGG
Cuts at: 2236 2536 2236
Size: 300 6046
BstYI r'GATC_y
Cuts at: 1972 2283 3345 3676 4043 4428 4445 5897 5983
Size: 311 1062 331 367 385 17 1452 86
Cuts at: 5983 5995 6341 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1062 385 367 346 331 311
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2013 2554 2812 2844 3057 513
Size: 552 939 9 541 258 32 213 3802
Fragments arranged by size:
3802 939 552 541 258 213 32 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5607
Fragments arranged by size:
5607 683 56
BtsI GCAGTG_nn'
Cuts at: 2508 2523 3136 3275 3862 4656 4676 2508
Size: 15 613 139 587 794 20 4178
Fragments arranged by size:
4178 794 613 587 139 20 15
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6346
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6311
DraI TTT'AAA
Cuts at: 3650 3650
Size: 6346
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5353 809
Size: 507 4037 1802
Fragments arranged by size:
4037 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2226 2538
Size: 54 1425 50 275 18 177 219 312
Cuts at: 2538 2714 2829 3054 3793 4682 6311 8
Size: 176 115 225 739 889 1629 43
Fragments arranged by size:
1629 1425 889 739 312 275 225 219
177 176 115 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3054 3793 1537
Size: 275 1242 739 4090
Fragments arranged by size:
4090 1242 739 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4276 1353
Size: 2923 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4933 5307 5453 387
Size: 1064 138 3344 374 146 1280
Fragments arranged by size:
3344 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3453 3453
Size: 6346
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2160 3068 3307 3517 4409 5006 5793
Size: 393 528 908 239 210 892 597 787
Cuts at: 5793 6061 1239
Size: 268 1524
Fragments arranged by size:
1524 908 892 787 597 528 393 268
239 210
EcoNI CCTnn'n_nnAGG
Cuts at: 2213 2213
Size: 6346
EcoO109I rG'GnC_Cy
Cuts at: 2040 2145 2951 2040
Size: 105 806 5435
Fragments arranged by size:
5435 806 105
EcoRI G'AATT_C
Cuts at: 3655 4033 3655
Size: 378 5968
FspI TGC'GCA
Cuts at: 4852 4852
Size: 6346
HaeII r_GCGC'y
Cuts at: 1678 2777 5493 1678
Size: 1099 2716 2531
Fragments arranged by size:
2716 2531 1099
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2420 2452 3018 3050 3568
Size: 32 135 32 848 32 566 32 518
Cuts at: 3568 3600 4987 5019 5061 5093 1373
Size: 32 1387 32 42 32 2626
Fragments arranged by size:
2626 1387 848 566 518 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3361 3690 3936 669
Size: 988 281 1423 329 246 3079
Fragments arranged by size:
3079 1423 988 329 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6346
HpaI GTT'AAC
Cuts at: 3936 3936
Size: 6346
KpnI G_GTAC'C
Cuts at: 2021 3671 2021
Size: 1650 4696
MfeI C'AATT_G
Cuts at: 3945 3945
Size: 6346
MluI A'CGCG_T
Cuts at: 6222 6222
Size: 6346
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2261 5460 5644 1305
Size: 956 3199 184 2007
Fragments arranged by size:
3199 2007 956 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2228 2540 10
Size: 54 1425 343 177 219 312 3816
Fragments arranged by size:
3816 1425 343 312 219 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2339 2459 3599 4304 4663 4822 518
Size: 648 1173 120 1140 705 359 159 2042
Fragments arranged by size:
2042 1173 1140 705 648 359 159 120
MspA1I CmG'CkG
Cuts at: 1606 2516 3059 3184 3762 4439 5587 5832 1606
Size: 910 543 125 578 677 1148 245 2120
Fragments arranged by size:
2120 1148 910 677 578 543 245 125
NaeI GCC'GGC
Cuts at: 2492 2492
Size: 6346
NcoI C'CATG_G
Cuts at: 513 1065 2004 2013 513
Size: 552 939 9 4846
Fragments arranged by size:
4846 939 552 9
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6346
NgoMIV G'CCGG_C
Cuts at: 2490 2490
Size: 6346
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6346
NotI GC'GGCC_GC
Cuts at: 3793 3793
Size: 6346
NspI r_CATG'y
Cuts at: 1132 1229 2593 3225 3704 5249 1132
Size: 97 1364 632 479 1545 2229
Fragments arranged by size:
2229 1545 1364 632 479 97
PasI CC'CwG_GG
Cuts at: 1348 2037 2599 3493 1348
Size: 689 562 894 4201
Fragments arranged by size:
4201 894 689 562
PciI A'CATG_T
Cuts at: 5245 5245
Size: 6346
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2605 1108
Size: 1497 4849
PfoI T'CCnGG_A
Cuts at: 2352 2352
Size: 6346
PmeI GTTT'AAAC
Cuts at: 3650 3650
Size: 6346
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4415 4447 5928 5960 4415
Size: 32 1481 32 4801
Fragments arranged by size:
4801 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2145 2951 2145
Size: 806 5540
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6346
PsiI TTA'TAA
Cuts at: 3916 3916
Size: 6346
PspXI vC'TCGA_Gb
Cuts at: 3024 3024
Size: 6346
PstI C_TGCA'G
Cuts at: 830 2165 3131 3270 3698 830
Size: 1335 966 139 428 3478
Fragments arranged by size:
3478 1335 966 428 139
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6346
PvuII CAG'CTG
Cuts at: 3184 3184
Size: 6346
SacI G_AGCT'C
Cuts at: 3455 3455
Size: 6346
SacII CC_GC'GG
Cuts at: 3060 3060
Size: 6346
SalI G'TCGA_C
Cuts at: 1936 3688 1936
Size: 1752 4594
SanDI GG'GwC_CC
Cuts at: 2145 2145
Size: 6346
SbfI CC_TGCA'GG
Cuts at: 3698 3698
Size: 6346
ScaI AGT'ACT
Cuts at: 2167 4594 6306 2167
Size: 2427 1712 2207
Fragments arranged by size:
2427 2207 1712
SfcI C'TryA_G
Cuts at: 826 1045 2161 3127 3266 3694 4829 5510 5701
Size: 219 1116 966 139 428 1135 681 191
Cuts at: 5701 826
Size: 1471
Fragments arranged by size:
1471 1135 1116 966 681 428 219 191
139
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6346
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6346
SmaI CCC'GGG
Cuts at: 1872 2873 2921 3066 3673 1872
Size: 1001 48 145 607 4545
Fragments arranged by size:
4545 1001 607 145 48
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3024 3140 4450 5351 5613 820
Size: 197 926 1081 116 1310 901 262 1553
Fragments arranged by size:
1553 1310 1081 926 901 262 197 116
SnaBI TAC'GTA
Cuts at: 493 4137 493
Size: 3644 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6346
SphI G_CATG'C
Cuts at: 1132 3704 1132
Size: 2572 3774
SspI AAT'ATT
Cuts at: 5 52 4270 5
Size: 47 4218 2081
Fragments arranged by size:
4218 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6346
StyI C'CwwG_G
Cuts at: 513 1065 2004 2013 2196 2384 2896 2947 3785
Size: 552 939 9 183 188 512 51 838
Cuts at: 3785 513
Size: 3074
Fragments arranged by size:
3074 939 838 552 512 188 183 51
9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3228 4373 4390 4543 4728 5173 325
Size: 1255 1648 1145 17 153 185 445 1498
Fragments arranged by size:
1648 1498 1255 1145 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2165 2298 2343
Size: 274 80 33 51 1443 188 133 45
Cuts at: 2343 4592 6304 96
Size: 2249 1712 138
Fragments arranged by size:
2249 1712 1443 274 188 138 133 80
51 45 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2601 2633 2696 2728 2943 2975 6306 6338 2601
Size: 32 63 32 215 32 3331 32 2609
Fragments arranged by size:
3331 2609 215 63 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6346
XbaI T'CTAG_A
Cuts at: 3682 3682
Size: 6346
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2028 2429 2455 3142 2011
Size: 17 401 26 687 5215
Fragments arranged by size:
5215 687 401 26 17
XhoI C'TCGA_G
Cuts at: 1943 3024 3140 1943
Size: 1081 116 5149
Fragments arranged by size:
5149 1081 116
XmaI C'CCGG_G
Cuts at: 1870 2871 2919 3064 3671 1870
Size: 1001 48 145 607 4545
Fragments arranged by size:
4545 1001 607 145 48
XmnI GAAnn'nnTTC
Cuts at: 4475 4475
Size: 6346
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2205 4154 276
Size: 53 83 186 936 671 1949 2468
Fragments arranged by size:
2468 1949 936 671 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI
PstI PvuI PvuII SacI SacII SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BbeI BfrBI BplI BsiWI
BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PmlI PspOMI PsrI RsrII
SapI SexAI SfiI SfoI SrfI SwaI