Restriction map for
pFN21AB5951
(Circular) (Six-base) MAPSORT of: pFN21AB5951.seq Check: 4491 from: 1 to: 6235
pFN21AB5951 6235 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4045 278
Size: 53 83 186 936 2509 2468
Fragments arranged by size:
2509 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3336 3578 1937
Size: 1399 242 4594
Fragments arranged by size:
4594 1399 242
Acc65I G'GTAC_C
Cuts at: 3556 3556
Size: 6235
AclI AA'CG_TT
Cuts at: 1563 4362 4735 1563
Size: 2799 373 3063
Fragments arranged by size:
3063 2799 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2154 2202 3092 3425 4298 5682 5950 1632
Size: 522 48 890 333 873 1384 268 1917
Fragments arranged by size:
1917 1384 890 873 522 333 268 48
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6038
AflIII A'CryG_T
Cuts at: 1203 5134 6111 1203
Size: 3931 977 1327
Fragments arranged by size:
3931 1327 977
AgeI A'CCGG_T
Cuts at: 5066 5066
Size: 6235
AhdI GACnn_n'nnGTC
Cuts at: 4964 4964
Size: 6235
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6235
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2511 2592 1184
Size: 1327 81 4827
Fragments arranged by size:
4827 1327 81
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2196 2228 2742 2774 2811 2843 2196
Size: 32 514 32 37 32 5588
Fragments arranged by size:
5588 514 37 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2555 2605 3124 3978 5550 2555
Size: 50 519 854 1572 3240
Fragments arranged by size:
3240 1572 854 519 50
ApaI G_GGCC'C
Cuts at: 2525 2847 3084 2525
Size: 322 237 5676
Fragments arranged by size:
5676 322 237
ApaLI G'TGCA_C
Cuts at: 4292 5448 4292
Size: 1156 5079
ApoI r'AATT_y
Cuts at: 1493 3341 3544 3770 3922 6031 1493
Size: 1848 203 226 152 2109 1697
Fragments arranged by size:
2109 1848 1697 226 203 152
AseI AT'TA_AT
Cuts at: 160 1032 4789 160
Size: 872 3757 1606
Fragments arranged by size:
3757 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6235
AvaI C'yCGr_G
Cuts at: 1870 1943 2830 2892 3560 1870
Size: 73 887 62 668 4545
Fragments arranged by size:
4545 887 668 73 62
BamHI G'GATC_C
Cuts at: 3565 3932 3565
Size: 367 5868
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3556 5011 618
Size: 325 217 627 1769 1455 1842
Fragments arranged by size:
1842 1769 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2525 2847 3084 3554 1391
Size: 1134 322 237 470 4072
Fragments arranged by size:
4072 1134 470 322 237
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3471 928
Size: 971 1572 3692
Fragments arranged by size:
3692 1572 971
BbvCI CC'TCA_GC
Cuts at: 2697 3428 2697
Size: 731 5504
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2873 2907 3509 3543 4426 4460 1903
Size: 34 936 34 602 34 883 34 3678
Fragments arranged by size:
3678 936 883 602 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2649 4127 5337 2649
Size: 1478 1210 3547
Fragments arranged by size:
3547 1478 1210
BclI T'GATC_A
Cuts at: 1548 2020 1548
Size: 472 5763
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4846 136
Size: 107 122 71 631 605 3174 1525
Fragments arranged by size:
3174 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2116 6230 2116
Size: 4114 2121
BlpI GC'TnA_GC
Cuts at: 3269 3652 3269
Size: 383 5852
Bme1580I G_kGCm'C
Cuts at: 1790 2525 2847 3084 4296 5452 1790
Size: 735 322 237 1212 1156 2573
Fragments arranged by size:
2573 1212 1156 735 322 237
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6235
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2501 2721 3966
Size: 416 53 83 399 24 1084 220 1245
Cuts at: 3966 4924 442
Size: 958 1753
Fragments arranged by size:
1753 1245 1084 958 416 399 220 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6235
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3294 3294
Size: 6235
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2733 2944 3342 4895 1239
Size: 1494 211 398 1553 2579
Fragments arranged by size:
2579 1553 1494 398 211
Bpu10I CC'TnA_GC
Cuts at: 2697 3428 2697
Size: 731 5504
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4360 5225 5523 4360
Size: 865 298 5072
Fragments arranged by size:
5072 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4898 882
Size: 617 3399 2219
Fragments arranged by size:
3399 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4026 493
Size: 711 2822 2702
Fragments arranged by size:
2822 2702 711
BsaBI GATnn'nnATC
Cuts at: 6229 6229
Size: 6235
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4042 4424 5059 275
Size: 53 83 186 936 2509 382 635 1451
Fragments arranged by size:
2509 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3205 4668 5066 5340 5487 5898 3205
Size: 1463 398 274 147 411 3542
Fragments arranged by size:
3542 1463 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2273 2303 1178
Size: 30 165 30 870 30 5110
Fragments arranged by size:
5110 870 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2281 2685 3449 1186
Size: 1095 404 764 3972
Fragments arranged by size:
3972 1095 764 404
BseYI C'CCAG_C
Cuts at: 2848 3145 3225 5438 5934 2848
Size: 297 80 2213 496 3149
Fragments arranged by size:
3149 2213 496 297 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2264 1163
Size: 573 528 5134
Fragments arranged by size:
5134 573 528
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3685 4446 5474 1540
Size: 275 186 1684 761 1028 2301
Fragments arranged by size:
2301 1684 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4296 4381 5452 4296
Size: 85 1071 5079
Fragments arranged by size:
5079 1071 85
BsmI GAATG_Cn'
Cuts at: 2072 3746 3839 2072
Size: 1674 93 4468
Fragments arranged by size:
4468 1674 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2525 2847 3084 3554 4296 4381 5452
Size: 399 735 322 237 470 742 85 1071
Cuts at: 5452 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 735 470 399 322 237
85
BspEI T'CCGG_A
Cuts at: 5898 5898
Size: 6235
BspHI T'CATG_A
Cuts at: 4124 5854 4124
Size: 1730 4505
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3590 844
Size: 2746 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3276 4122 1394
Size: 429 1453 846 3507
Fragments arranged by size:
3507 1453 846 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3162 4730 4904 65
Size: 1183 1914 1568 174 1396
Fragments arranged by size:
1914 1568 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3243 4879 5066 1700
Size: 110 1433 1636 187 2869
Fragments arranged by size:
2869 1636 1433 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6235
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6235
BssSI C'ACGA_G
Cuts at: 4295 5307 4295
Size: 1012 5223
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6235
BstBI TT'CG_AA
Cuts at: 5039 5039
Size: 6235
BstEII G'GTnAC_C
Cuts at: 1184 3127 1184
Size: 1943 4292
BstYI r'GATC_y
Cuts at: 1972 2116 2149 2992 3565 3932 4317 4334 5786
Size: 144 33 843 573 367 385 17 1452
Cuts at: 5786 5872 5884 6230 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 843 573 385 367 346 144
86 33 17 12
Bsu36I CC'TnA_GG
Cuts at: 2463 2482 2781 2463
Size: 19 299 5917
Fragments arranged by size:
5917 299 19
BtgI C'CryG_G
Cuts at: 513 1065 2004 3372 513
Size: 552 939 1368 3376
Fragments arranged by size:
3376 1368 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5496
Fragments arranged by size:
5496 683 56
BtsI GCAGTG_nn'
Cuts at: 3751 4545 4565 3751
Size: 794 20 5421
Fragments arranged by size:
5421 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6235
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6200
DraI TTT'AAA
Cuts at: 3539 3539
Size: 6235
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5242 809
Size: 507 3926 1802
Fragments arranged by size:
3926 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3245 3682 4571
Size: 54 1425 50 275 18 1415 437 889
Cuts at: 4571 6200 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1415 889 437 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3682 1537
Size: 275 1870 4090
Fragments arranged by size:
4090 1870 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2130 3204 3371 4165 1353
Size: 777 1074 167 794 3423
Fragments arranged by size:
3423 1074 794 777 167
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4822 5196 5342 387
Size: 1064 138 3233 374 146 1280
Fragments arranged by size:
3233 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2154 2202 2733 2944 3092 3342 3425
Size: 393 522 48 531 211 148 250 83
Cuts at: 3425 4298 4895 5682 5950 1239
Size: 873 597 787 268 1524
Fragments arranged by size:
1524 873 787 597 531 522 393 268
250 211 148 83 48
EcoNI CCTnn'n_nnAGG
Cuts at: 2374 2700 3431 2374
Size: 326 731 5178
Fragments arranged by size:
5178 731 326
EcoO109I rG'GnC_Cy
Cuts at: 2522 2827 2843 2844 3080 3081 2522
Size: 305 16 1 236 1 5676
Fragments arranged by size:
5676 305 236 16 1 1
EcoRI G'AATT_C
Cuts at: 3544 3922 3544
Size: 378 5857
FspI TGC'GCA
Cuts at: 4741 4741
Size: 6235
HaeII r_GCGC'y
Cuts at: 1678 3226 5382 1678
Size: 1548 2156 2531
Fragments arranged by size:
2531 2156 1548
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2210 2242 2288 2320 4876
Size: 32 135 32 638 32 46 32 2556
Cuts at: 4876 4908 4950 4982 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2556 638 135 46 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2159 3579 3825 669
Size: 988 281 221 1420 246 3079
Fragments arranged by size:
3079 1420 988 281 246 221
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6235
HpaI GTT'AAC
Cuts at: 3825 3825
Size: 6235
KpnI G_GTAC'C
Cuts at: 3560 3560
Size: 6235
MfeI C'AATT_G
Cuts at: 3834 3834
Size: 6235
MluI A'CGCG_T
Cuts at: 6111 6111
Size: 6235
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2282 5349 5533 1305
Size: 977 3067 184 2007
Fragments arranged by size:
3067 2007 977 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4413
Fragments arranged by size:
4413 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4193 4552 4711 518
Size: 648 3027 359 159 2042
Fragments arranged by size:
3027 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2501 3112 3451 3498 3651 4328 5476 5721
Size: 895 611 339 47 153 677 1148 245
Cuts at: 5721 1606
Size: 2120
Fragments arranged by size:
2120 1148 895 677 611 339 245 153
47
NcoI C'CATG_G
Cuts at: 513 1065 2004 3372 513
Size: 552 939 1368 3376
Fragments arranged by size:
3376 1368 939 552
NdeI CA'TA_TG
Cuts at: 387 2983 387
Size: 2596 3639
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6235
NotI GC'GGCC_GC
Cuts at: 3682 3682
Size: 6235
NspI r_CATG'y
Cuts at: 1132 1229 2492 2839 3593 5138 1132
Size: 97 1263 347 754 1545 2229
Fragments arranged by size:
2229 1545 1263 754 347 97
PasI CC'CwG_GG
Cuts at: 1348 2045 1348
Size: 697 5538
PciI A'CATG_T
Cuts at: 5134 5134
Size: 6235
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2555 1108
Size: 1447 4788
PfoI T'CCnGG_A
Cuts at: 2710 3381 3483 2710
Size: 671 102 5462
Fragments arranged by size:
5462 671 102
PmeI GTTT'AAAC
Cuts at: 3539 3539
Size: 6235
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3085 3117 4304 4336 5817 5849 3085
Size: 32 1187 32 1481 32 3471
Fragments arranged by size:
3471 1481 1187 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2827 2827
Size: 6235
PshAI GACnn'nnGTC
Cuts at: 1613 2668 1613
Size: 1055 5180
PsiI TTA'TAA
Cuts at: 3805 3805
Size: 6235
PspOMI G'GGCC_C
Cuts at: 2521 2843 3080 2521
Size: 322 237 5676
Fragments arranged by size:
5676 322 237
PstI C_TGCA'G
Cuts at: 830 2500 2610 2767 3503 3587 830
Size: 1670 110 157 736 84 3478
Fragments arranged by size:
3478 1670 736 157 110 84
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6235
PvuII CAG'CTG
Cuts at: 2501 3112 2501
Size: 611 5624
SalI G'TCGA_C
Cuts at: 1936 3577 1936
Size: 1641 4594
SanDI GG'GwC_CC
Cuts at: 2827 2827
Size: 6235
SapI GCTCTTCn'nnn_
Cuts at: 2130 2130
Size: 6235
SbfI CC_TGCA'GG
Cuts at: 3587 3587
Size: 6235
ScaI AGT'ACT
Cuts at: 4483 6195 4483
Size: 1712 4523
SexAI A'CCwGG_T
Cuts at: 2235 2235
Size: 6235
SfcI C'TryA_G
Cuts at: 826 1045 2496 2606 2763 3499 3583 4718 5399
Size: 219 1451 110 157 736 84 1135 681
Cuts at: 5399 5590 826
Size: 191 1471
Fragments arranged by size:
1471 1451 1135 736 681 219 191 157
110 84
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6235
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6235
SmaI CCC'GGG
Cuts at: 1872 2832 3562 1872
Size: 960 730 4545
Fragments arranged by size:
4545 960 730
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4339 5240 5502 820
Size: 197 926 2396 901 262 1553
Fragments arranged by size:
2396 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4026 493
Size: 3533 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6235
SphI G_CATG'C
Cuts at: 1132 2492 2839 3593 1132
Size: 1360 347 754 3774
Fragments arranged by size:
3774 1360 754 347
SspI AAT'ATT
Cuts at: 5 52 4159 5
Size: 47 4107 2081
Fragments arranged by size:
4107 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6235
StyI C'CwwG_G
Cuts at: 513 1065 2004 3372 3674 513
Size: 552 939 1368 302 3074
Fragments arranged by size:
3074 1368 939 552 302
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2446 2450 4262 4279 4432 4617 5062
Size: 1255 866 4 1812 17 153 185 445
Cuts at: 5062 325
Size: 1498
Fragments arranged by size:
1812 1498 1255 866 445 185 153 17
4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4481 6193 96
Size: 274 80 33 51 1443 2504 1712 138
Fragments arranged by size:
2504 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2273 2305 2844 2876 6195 6227 2273
Size: 32 539 32 3319 32 2281
Fragments arranged by size:
3319 2281 539 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6235
XbaI T'CTAG_A
Cuts at: 3571 3571
Size: 6235
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6235
XmaI C'CCGG_G
Cuts at: 1870 2830 3560 1870
Size: 960 730 4545
Fragments arranged by size:
4545 960 730
XmnI GAAnn'nnTTC
Cuts at: 4364 4364
Size: 6235
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4043 276
Size: 53 83 186 936 2509 2468
Fragments arranged by size:
2509 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI RsrII SacI SacII SfiI SfoI
SrfI SwaI XcmI