Restriction map for
pFN21AB5949
(Circular) (Six-base) MAPSORT of: pFN21AB5949.seq Check: 424 from: 1 to: 6232
pFN21AB5949 6232 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4042 278
Size: 53 83 186 936 2506 2468
Fragments arranged by size:
2506 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3575 1937
Size: 1638 4594
Acc65I G'GTAC_C
Cuts at: 3553 3553
Size: 6232
AclI AA'CG_TT
Cuts at: 1563 4359 4732 1563
Size: 2796 373 3063
Fragments arranged by size:
3063 2796 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2820 3300 4295 5679 5947 1632
Size: 1188 480 995 1384 268 1917
Fragments arranged by size:
1917 1384 1188 995 480 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6035
AflIII A'CryG_T
Cuts at: 1203 3469 5131 6108 1203
Size: 2266 1662 977 1327
Fragments arranged by size:
2266 1662 1327 977
AgeI A'CCGG_T
Cuts at: 5063 5063
Size: 6232
AhdI GACnn_n'nnGTC
Cuts at: 4961 4961
Size: 6232
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6232
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2478 1184
Size: 1294 4938
AlwNI CAG_nnn'CTG
Cuts at: 2215 3043 3975 5547 2215
Size: 828 932 1572 2900
Fragments arranged by size:
2900 1572 932 828
ApaLI G'TGCA_C
Cuts at: 2688 4289 5445 2688
Size: 1601 1156 3475
Fragments arranged by size:
3475 1601 1156
ApoI r'AATT_y
Cuts at: 1493 2330 3132 3541 3767 3919 6028 1493
Size: 837 802 409 226 152 2109 1697
Fragments arranged by size:
2109 1697 837 802 409 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2510 4786 160
Size: 872 1478 2276 1606
Fragments arranged by size:
2276 1606 1478 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6232
AvaI C'yCGr_G
Cuts at: 1870 1943 3557 1870
Size: 73 1614 4545
Fragments arranged by size:
4545 1614 73
BamHI G'GATC_C
Cuts at: 3562 3929 3562
Size: 367 5865
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3553 5008 618
Size: 325 217 627 1766 1455 1842
Fragments arranged by size:
1842 1766 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2405 3551 1391
Size: 1014 1146 4072
Fragments arranged by size:
4072 1146 1014
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3009 3171 3286 928
Size: 971 1110 162 115 3874
Fragments arranged by size:
3874 1110 971 162 115
BbvCI CC'TCA_GC
Cuts at: 2038 2038
Size: 6232
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2507 2541 4423 4457 1903
Size: 34 570 34 1882 34 3678
Fragments arranged by size:
3678 1882 570 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4124 5334 4124
Size: 1210 5022
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6232
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2048 4843
Size: 107 122 71 631 605 337 39 2795
Cuts at: 4843 136
Size: 1525
Fragments arranged by size:
2795 1525 631 605 337 122 107 71
39
BglII A'GATC_T
Cuts at: 3052 6227 3052
Size: 3175 3057
BlpI GC'TnA_GC
Cuts at: 3649 3649
Size: 6232
Bme1580I G_kGCm'C
Cuts at: 1790 2692 4293 5449 1790
Size: 902 1601 1156 2573
Fragments arranged by size:
2573 1601 1156 902
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6232
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3963 4921 442
Size: 416 53 83 399 24 2546 958 1753
Fragments arranged by size:
2546 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6232
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2094 2212 2337 3377 4892 1239
Size: 855 118 125 1040 1515 2579
Fragments arranged by size:
2579 1515 1040 855 125 118
Bpu10I CC'TnA_GC
Cuts at: 2038 2707 2038
Size: 669 5563
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4357 5222 5520 4357
Size: 865 298 5069
Fragments arranged by size:
5069 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4895 882
Size: 617 3396 2219
Fragments arranged by size:
3396 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3425 4023 493
Size: 711 2221 598 2702
Fragments arranged by size:
2702 2221 711 598
BsaBI GATnn'nnATC
Cuts at: 6226 6226
Size: 6232
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4039 4421 5056 275
Size: 53 83 186 936 2506 382 635 1451
Fragments arranged by size:
2506 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4665 5063 5337 5484 5895 4665
Size: 398 274 147 411 5002
Fragments arranged by size:
5002 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6007
Fragments arranged by size:
6007 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6232
BseYI C'CCAG_C
Cuts at: 3061 5435 5931 3061
Size: 2374 496 3362
Fragments arranged by size:
3362 2374 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2017 3135 1163
Size: 573 281 1118 4260
Fragments arranged by size:
4260 1118 573 281
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3682 4443 5471 1540
Size: 275 186 1681 761 1028 2301
Fragments arranged by size:
2301 1681 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2692 4293 4378 5449 2692
Size: 1601 85 1071 3475
Fragments arranged by size:
3475 1601 1071 85
BsmI GAATG_Cn'
Cuts at: 3743 3836 3743
Size: 93 6139
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2405 2692 3551 4293 4378 5449 1391
Size: 399 615 287 859 742 85 1071 2174
Fragments arranged by size:
2174 1071 859 742 615 399 287 85
BspEI T'CCGG_A
Cuts at: 5895 5895
Size: 6232
BspHI T'CATG_A
Cuts at: 4121 5851 4121
Size: 1730 4502
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3587 844
Size: 2743 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4119 1394
Size: 429 2296 3507
Fragments arranged by size:
3507 2296 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2890 3290 4727 4901 65
Size: 1183 1642 400 1437 174 1396
Fragments arranged by size:
1642 1437 1396 1183 400 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2093 4876 5063 1700
Size: 110 283 2783 187 2869
Fragments arranged by size:
2869 2783 283 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6232
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6232
BssSI C'ACGA_G
Cuts at: 4292 5304 4292
Size: 1012 5220
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2090 794
Size: 1296 4936
BstBI TT'CG_AA
Cuts at: 5036 5036
Size: 6232
BstEII G'GTnAC_C
Cuts at: 1184 2096 1184
Size: 912 5320
BstYI r'GATC_y
Cuts at: 1972 2211 2738 3052 3461 3562 3929 4314 4331
Size: 239 527 314 409 101 367 385 17
Cuts at: 4331 5783 5869 5881 6227 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 527 409 385 367 346 314
239 101 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4741
Fragments arranged by size:
4741 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2239 487
Size: 683 56 1013 4480
Fragments arranged by size:
4480 1013 683 56
BtsI GCAGTG_nn'
Cuts at: 3748 4542 4562 3748
Size: 794 20 5418
Fragments arranged by size:
5418 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6232
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6197
DraI TTT'AAA
Cuts at: 3536 3536
Size: 6232
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5239 809
Size: 507 3923 1802
Fragments arranged by size:
3923 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 2049 2065
Size: 54 1425 50 275 18 180 39 16
Cuts at: 2065 3679 4568 6197 8
Size: 1614 889 1629 43
Fragments arranged by size:
1629 1614 1425 889 275 180 54 50
43 39 18 16
EagI C'GGCC_G
Cuts at: 1537 1812 3679 1537
Size: 275 1867 4090
Fragments arranged by size:
4090 1867 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2133 2538 4162 1353
Size: 780 405 1624 3423
Fragments arranged by size:
3423 1624 780 405
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4819 5193 5339 387
Size: 1064 138 3230 374 146 1280
Fragments arranged by size:
3230 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2094 2212 2337 2820 3300 3377 4295
Size: 393 462 118 125 483 480 77 918
Cuts at: 4295 4892 5679 5947 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 918 787 597 483 480 462 393
268 125 118 77
EcoRI G'AATT_C
Cuts at: 3132 3541 3919 3132
Size: 409 378 5445
Fragments arranged by size:
5445 409 378
EcoRV GAT'ATC
Cuts at: 2989 2989
Size: 6232
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3188 3330 3188
Size: 142 6090
FspI TGC'GCA
Cuts at: 4738 4738
Size: 6232
HaeII r_GCGC'y
Cuts at: 1678 5379 1678
Size: 3701 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3037 3069 3512 3544 4873
Size: 32 135 32 1465 32 443 32 1329
Cuts at: 4873 4905 4947 4979 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1465 1329 443 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2338 2635 2823 3576 3822 669
Size: 988 281 400 297 188 753 246 3079
Fragments arranged by size:
3079 988 753 400 297 281 246 188
HindIII A'AGCT_T
Cuts at: 748 2594 748
Size: 1846 4386
HpaI GTT'AAC
Cuts at: 2338 2635 3822 2338
Size: 297 1187 4748
Fragments arranged by size:
4748 1187 297
KpnI G_GTAC'C
Cuts at: 3557 3557
Size: 6232
MfeI C'AATT_G
Cuts at: 3831 3831
Size: 6232
MluI A'CGCG_T
Cuts at: 6108 6108
Size: 6232
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2128 5346 5530 1305
Size: 823 3218 184 2007
Fragments arranged by size:
3218 2007 823 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4410
Fragments arranged by size:
4410 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4190 4549 4708 518
Size: 648 3024 359 159 2042
Fragments arranged by size:
3024 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2114 3065 3648 4325 5473 5718 1606
Size: 508 951 583 677 1148 245 2120
Fragments arranged by size:
2120 1148 951 677 583 508 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4741
Fragments arranged by size:
4741 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6232
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6232
NotI GC'GGCC_GC
Cuts at: 3679 3679
Size: 6232
NspI r_CATG'y
Cuts at: 1132 1229 3473 3480 3590 5135 1132
Size: 97 2244 7 110 1545 2229
Fragments arranged by size:
2244 2229 1545 110 97 7
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6232
PciI A'CATG_T
Cuts at: 3469 5131 3469
Size: 1662 4570
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6232
PfoI T'CCnGG_A
Cuts at: 3393 3393
Size: 6232
PmeI GTTT'AAAC
Cuts at: 3536 3536
Size: 6232
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4301 4333 5814 5846 4301
Size: 32 1481 32 4687
Fragments arranged by size:
4687 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6232
PsiI TTA'TAA
Cuts at: 3802 3802
Size: 6232
PstI C_TGCA'G
Cuts at: 830 2530 3584 830
Size: 1700 1054 3478
Fragments arranged by size:
3478 1700 1054
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6232
PvuII CAG'CTG
Cuts at: 2114 3065 2114
Size: 951 5281
SalI G'TCGA_C
Cuts at: 1936 3574 1936
Size: 1638 4594
SapI GCTCTTCn'nnn_
Cuts at: 2538 2538
Size: 6232
SbfI CC_TGCA'GG
Cuts at: 3584 3584
Size: 6232
ScaI AGT'ACT
Cuts at: 4480 6192 4480
Size: 1712 4520
SfcI C'TryA_G
Cuts at: 826 1045 2526 3035 3120 3580 4715 5396 5587
Size: 219 1481 509 85 460 1135 681 191
Cuts at: 5587 826
Size: 1471
Fragments arranged by size:
1481 1471 1135 681 509 460 219 191
85
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6232
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6232
SmaI CCC'GGG
Cuts at: 1872 3559 1872
Size: 1687 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4336 5237 5499 820
Size: 197 926 2393 901 262 1553
Fragments arranged by size:
2393 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4023 493
Size: 3530 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6232
SphI G_CATG'C
Cuts at: 1132 3590 1132
Size: 2458 3774
SspI AAT'ATT
Cuts at: 5 52 2182 2581 4156 5
Size: 47 2130 399 1575 2081
Fragments arranged by size:
2130 2081 1575 399 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6232
StyI C'CwwG_G
Cuts at: 513 1065 2004 2826 3671 513
Size: 552 939 822 845 3074
Fragments arranged by size:
3074 939 845 822 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2115 4259 4276 4429 4614 5059 325
Size: 1255 535 2144 17 153 185 445 1498
Fragments arranged by size:
2144 1498 1255 535 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3388 4478 6190
Size: 274 80 33 51 1443 1411 1090 1712
Cuts at: 6190 96
Size: 138
Fragments arranged by size:
1712 1443 1411 1090 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6192 6224 6192
Size: 32 6200
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6232
XbaI T'CTAG_A
Cuts at: 3568 3568
Size: 6232
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6232
XmaI C'CCGG_G
Cuts at: 1870 3557 1870
Size: 1687 4545
XmnI GAAnn'nnTTC
Cuts at: 2182 4361 2182
Size: 2179 4053
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4040 276
Size: 53 83 186 936 2506 2468
Fragments arranged by size:
2506 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI