Restriction map for
pFN21AB5947
(Circular) (Six-base) MAPSORT of: pFN21AB5947.seq Check: 842 from: 1 to: 6232
pFN21AB5947 6232 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4042 278
Size: 53 83 186 936 2506 2468
Fragments arranged by size:
2506 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3575 1937
Size: 1638 4594
Acc65I G'GTAC_C
Cuts at: 3553 3553
Size: 6232
AclI AA'CG_TT
Cuts at: 1563 4359 4732 1563
Size: 2796 373 3063
Fragments arranged by size:
3063 2796 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2625 2759 2849 2982 4295 5679 5947 1632
Size: 993 134 90 133 1313 1384 268 1917
Fragments arranged by size:
1917 1384 1313 993 268 134 133 90
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6035
AflIII A'CryG_T
Cuts at: 1203 5131 6108 1203
Size: 3928 977 1327
Fragments arranged by size:
3928 1327 977
AgeI A'CCGG_T
Cuts at: 5063 5063
Size: 6232
AhdI GACnn_n'nnGTC
Cuts at: 4961 4961
Size: 6232
AleI CACnn'nnGTG
Cuts at: 1166 2810 1166
Size: 1644 4588
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6232
AlwNI CAG_nnn'CTG
Cuts at: 2311 2428 2902 3361 3975 5547 2311
Size: 117 474 459 614 1572 2996
Fragments arranged by size:
2996 1572 614 474 459 117
ApaLI G'TGCA_C
Cuts at: 2653 4289 5445 2653
Size: 1636 1156 3440
Fragments arranged by size:
3440 1636 1156
ApoI r'AATT_y
Cuts at: 1493 3541 3767 3919 6028 1493
Size: 2048 226 152 2109 1697
Fragments arranged by size:
2109 2048 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4786 160
Size: 872 3754 1606
Fragments arranged by size:
3754 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6232
AvaI C'yCGr_G
Cuts at: 1870 1943 2435 3557 1870
Size: 73 492 1122 4545
Fragments arranged by size:
4545 1122 492 73
BamHI G'GATC_C
Cuts at: 3285 3562 3929 3285
Size: 277 367 5588
Fragments arranged by size:
5588 367 277
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2431 3553 5008 618
Size: 325 217 627 644 1122 1455 1842
Fragments arranged by size:
1842 1455 1122 644 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2226 2376 2937 3551 1391
Size: 835 150 561 614 4072
Fragments arranged by size:
4072 835 614 561 150
BbeI G_GCGC'C
Cuts at: 2435 2435
Size: 6232
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5261
BbvCI CC'TCA_GC
Cuts at: 3525 3525
Size: 6232
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4423 4457 1903
Size: 34 2486 34 3678
Fragments arranged by size:
3678 2486 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4124 5334 4124
Size: 1210 5022
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6232
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2328 2861 4843
Size: 107 122 71 631 605 656 533 1982
Cuts at: 4843 136
Size: 1525
Fragments arranged by size:
1982 1525 656 631 605 533 122 107
71
BglII A'GATC_T
Cuts at: 2239 6227 2239
Size: 3988 2244
BlpI GC'TnA_GC
Cuts at: 3649 3649
Size: 6232
Bme1580I G_kGCm'C
Cuts at: 1790 2108 2657 2805 4293 5449 1790
Size: 318 549 148 1488 1156 2573
Fragments arranged by size:
2573 1488 1156 549 318 148
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6232
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3963 4921 442
Size: 416 53 83 399 24 2546 958 1753
Fragments arranged by size:
2546 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6232
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2297 2390 3383 3506 4892 1239
Size: 1058 93 993 123 1386 2579
Fragments arranged by size:
2579 1386 1058 993 123 93
Bpu10I CC'TnA_GC
Cuts at: 2399 3131 3525 2399
Size: 732 394 5106
Fragments arranged by size:
5106 732 394
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2972 4357 5222 5520 2972
Size: 1385 865 298 3684
Fragments arranged by size:
3684 1385 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4895 882
Size: 617 3396 2219
Fragments arranged by size:
3396 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4023 493
Size: 711 2819 2702
Fragments arranged by size:
2819 2702 711
BsaBI GATnn'nnATC
Cuts at: 6226 6226
Size: 6232
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2432 2853 4039 4421
Size: 53 83 186 936 899 421 1186 382
Cuts at: 4421 5056 275
Size: 635 1451
Fragments arranged by size:
1451 1186 936 899 635 421 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4665 5063 5337 5484 5895 4665
Size: 398 274 147 411 5002
Fragments arranged by size:
5002 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2147 2177 2520 2550 2699
Size: 30 165 30 744 30 343 30 149
Cuts at: 2699 2729 1178
Size: 30 4681
Fragments arranged by size:
4681 744 343 165 149 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3419 1186
Size: 2233 3999
BseYI C'CCAG_C
Cuts at: 2755 5435 5931 2755
Size: 2680 496 3056
Fragments arranged by size:
3056 2680 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2195 1163
Size: 573 459 5200
Fragments arranged by size:
5200 573 459
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3316 3682 4443 5471 1540
Size: 275 186 1315 366 761 1028 2301
Fragments arranged by size:
2301 1315 1028 761 366 275 186
BsiHKAI G_wGCw'C
Cuts at: 2480 2657 4293 4378 5449 2480
Size: 177 1636 85 1071 3263
Fragments arranged by size:
3263 1636 1071 177 85
BsmI GAATG_Cn'
Cuts at: 3062 3743 3836 3062
Size: 681 93 5458
Fragments arranged by size:
5458 681 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2726 2726
Size: 6232
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2108 2226 2376 2480 2657 2805 2937
Size: 399 318 118 150 104 177 148 132
Cuts at: 2937 3551 4293 4378 5449 1391
Size: 614 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 614 399 318 177 150
148 132 118 104 85
BspEI T'CCGG_A
Cuts at: 5895 5895
Size: 6232
BspHI T'CATG_A
Cuts at: 2877 3420 4121 5851 2877
Size: 543 701 1730 3258
Fragments arranged by size:
3258 1730 701 543
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3480 3587 844
Size: 2636 107 3489
Fragments arranged by size:
3489 2636 107
BsrBI CCG'CTC
Cuts at: 1394 1823 2286 2419 2752 3076 4119 1394
Size: 429 463 133 333 324 1043 3507
Fragments arranged by size:
3507 1043 463 429 333 324 133
BsrDI GCAATG_nn'
Cuts at: 65 1248 4727 4901 65
Size: 1183 3479 174 1396
Fragments arranged by size:
3479 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2173 3453 4876 5063 1700
Size: 110 363 1280 1423 187 2869
Fragments arranged by size:
2869 1423 1280 363 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6232
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6232
BssSI C'ACGA_G
Cuts at: 4292 5304 4292
Size: 1012 5220
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6232
BstBI TT'CG_AA
Cuts at: 5036 5036
Size: 6232
BstEII G'GTnAC_C
Cuts at: 1184 2149 2923 1184
Size: 965 774 4493
Fragments arranged by size:
4493 965 774
BstXI CCAn_nnnn'nTGG
Cuts at: 3115 3115
Size: 6232
BstYI r'GATC_y
Cuts at: 1972 2239 2550 3285 3562 3929 4314 4331 5783
Size: 267 311 735 277 367 385 17 1452
Cuts at: 5783 5869 5881 6227 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 735 385 367 346 311 277
267 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2316 2481 2699 3310 513
Size: 552 939 312 165 218 611 3435
Fragments arranged by size:
3435 939 611 552 312 218 165
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5493
Fragments arranged by size:
5493 683 56
BtsI GCAGTG_nn'
Cuts at: 2183 3484 3748 4542 4562 2183
Size: 1301 264 794 20 3853
Fragments arranged by size:
3853 1301 794 264 20
ClaI AT'CG_AT
Cuts at: 1554 2055 1554
Size: 501 5731
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6197
DraI TTT'AAA
Cuts at: 3536 3536
Size: 6232
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5239 809
Size: 507 3923 1802
Fragments arranged by size:
3923 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2352 2862 3313
Size: 54 1425 50 275 18 522 510 451
Cuts at: 3313 3501 3679 4568 6197 8
Size: 188 178 889 1629 43
Fragments arranged by size:
1629 1425 889 522 510 451 275 188
178 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3313 3679 1537
Size: 275 1501 366 4090
Fragments arranged by size:
4090 1501 366 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2816 3066 4162 1353
Size: 1463 250 1096 3423
Fragments arranged by size:
3423 1463 1096 250
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2160 2612 4819 5193 5339 387
Size: 1064 138 571 452 2207 374 146 1280
Fragments arranged by size:
2207 1280 1064 571 452 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2297 2390 2625 2759 2849 2982 3383
Size: 393 665 93 235 134 90 133 401
Cuts at: 3383 3506 4295 4892 5679 5947 1239
Size: 123 789 597 787 268 1524
Fragments arranged by size:
1524 789 787 665 597 401 393 268
235 134 133 123 93 90
EcoNI CCTnn'n_nnAGG
Cuts at: 2831 2864 3170 3327 2831
Size: 33 306 157 5736
Fragments arranged by size:
5736 306 157 33
EcoO109I rG'GnC_Cy
Cuts at: 2140 2140
Size: 6232
EcoRI G'AATT_C
Cuts at: 3541 3919 3541
Size: 378 5854
FspI TGC'GCA
Cuts at: 3476 4738 3476
Size: 1262 4970
HaeII r_GCGC'y
Cuts at: 1678 2435 3230 3445 5379 1678
Size: 757 795 215 1934 2531
Fragments arranged by size:
2531 1934 795 757 215
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2542 2574 3260 3292 4873
Size: 32 135 32 970 32 686 32 1581
Cuts at: 4873 4905 4947 4979 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1581 970 686 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3012 3576 3822 669
Size: 988 281 1074 564 246 3079
Fragments arranged by size:
3079 1074 988 564 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6232
HpaI GTT'AAC
Cuts at: 3822 3822
Size: 6232
KasI G'GCGC_C
Cuts at: 2431 2431
Size: 6232
KpnI G_GTAC'C
Cuts at: 3557 3557
Size: 6232
MfeI C'AATT_G
Cuts at: 3831 3831
Size: 6232
MluI A'CGCG_T
Cuts at: 6108 6108
Size: 6232
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5346 5530 1305
Size: 4041 184 2007
Fragments arranged by size:
4041 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2354 10
Size: 54 1425 343 522 3888
Fragments arranged by size:
3888 1425 522 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2057 2217 2810 3116 4190 4549 4708
Size: 648 891 160 593 306 1074 359 159
Cuts at: 4708 518
Size: 2042
Fragments arranged by size:
2042 1074 891 648 593 359 306 160
159
MspA1I CmG'CkG
Cuts at: 1606 2483 2887 2899 3648 4325 5473 5718 1606
Size: 877 404 12 749 677 1148 245 2120
Fragments arranged by size:
2120 1148 877 749 677 404 245 12
NaeI GCC'GGC
Cuts at: 2175 2175
Size: 6232
NarI GG'CG_CC
Cuts at: 2432 2432
Size: 6232
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4741
Fragments arranged by size:
4741 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6232
NgoMIV G'CCGG_C
Cuts at: 2173 2173
Size: 6232
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6232
NotI GC'GGCC_GC
Cuts at: 3679 3679
Size: 6232
NspI r_CATG'y
Cuts at: 1132 1229 2845 3590 5135 1132
Size: 97 1616 745 1545 2229
Fragments arranged by size:
2229 1616 1545 745 97
PasI CC'CwG_GG
Cuts at: 1348 2727 3329 1348
Size: 1379 602 4251
Fragments arranged by size:
4251 1379 602
PciI A'CATG_T
Cuts at: 5131 5131
Size: 6232
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2096 2902 3219 1108
Size: 988 806 317 4121
Fragments arranged by size:
4121 988 806 317
PfoI T'CCnGG_A
Cuts at: 2274 2463 2505 3027 2274
Size: 189 42 522 5479
Fragments arranged by size:
5479 522 189 42
PmeI GTTT'AAAC
Cuts at: 3536 3536
Size: 6232
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4301 4333 5814 5846 4301
Size: 32 1481 32 4687
Fragments arranged by size:
4687 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2140 2140
Size: 6232
PshAI GACnn'nnGTC
Cuts at: 1613 3396 1613
Size: 1783 4449
PsiI TTA'TAA
Cuts at: 3802 3802
Size: 6232
PstI C_TGCA'G
Cuts at: 830 2021 3584 830
Size: 1191 1563 3478
Fragments arranged by size:
3478 1563 1191
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6232
PvuII CAG'CTG
Cuts at: 2887 2887
Size: 6232
RsrII CG'GwC_CG
Cuts at: 2503 2503
Size: 6232
SacII CC_GC'GG
Cuts at: 2484 2484
Size: 6232
SalI G'TCGA_C
Cuts at: 1936 3574 1936
Size: 1638 4594
SanDI GG'GwC_CC
Cuts at: 2140 2140
Size: 6232
SapI GCTCTTCn'nnn_
Cuts at: 2816 3066 2816
Size: 250 5982
SbfI CC_TGCA'GG
Cuts at: 3584 3584
Size: 6232
ScaI AGT'ACT
Cuts at: 4480 6192 4480
Size: 1712 4520
SexAI A'CCwGG_T
Cuts at: 2196 2196
Size: 6232
SfcI C'TryA_G
Cuts at: 826 1045 2017 3580 4715 5396 5587 826
Size: 219 972 1563 1135 681 191 1471
Fragments arranged by size:
1563 1471 1135 972 681 219 191
SfoI GGC'GCC
Cuts at: 2433 2433
Size: 6232
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6232
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6232
SmaI CCC'GGG
Cuts at: 1872 2437 3559 1872
Size: 565 1122 4545
Fragments arranged by size:
4545 1122 565
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2987 4336 5237 5499 820
Size: 197 926 1044 1349 901 262 1553
Fragments arranged by size:
1553 1349 1044 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4023 493
Size: 3530 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6232
SphI G_CATG'C
Cuts at: 1132 3590 1132
Size: 2458 3774
SspI AAT'ATT
Cuts at: 5 52 4156 5
Size: 47 4104 2081
Fragments arranged by size:
4104 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6232
StyI C'CwwG_G
Cuts at: 513 1065 2004 3671 513
Size: 552 939 1667 3074
Fragments arranged by size:
3074 1667 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2491 2942 3323 4259 4276 4429 4614
Size: 1255 911 451 381 936 17 153 185
Cuts at: 4614 5059 325
Size: 445 1498
Fragments arranged by size:
1498 1255 936 911 451 445 381 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3183 4478 6190
Size: 274 80 33 51 1443 1206 1295 1712
Cuts at: 6190 96
Size: 138
Fragments arranged by size:
1712 1443 1295 1206 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2487 2519 2520 2552 2699 2731 6192 6224 2487
Size: 32 1 32 147 32 3461 32 2495
Fragments arranged by size:
3461 2495 147 32 32 32 32 1
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6232
XbaI T'CTAG_A
Cuts at: 3568 3568
Size: 6232
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2089 2089
Size: 6232
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6232
XmaI C'CCGG_G
Cuts at: 1870 2435 3557 1870
Size: 565 1122 4545
Fragments arranged by size:
4545 1122 565
XmnI GAAnn'nnTTC
Cuts at: 4361 4361
Size: 6232
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4040 276
Size: 53 83 186 936 2506 2468
Fragments arranged by size:
2506 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI
PsrI SacI SfiI SrfI SwaI