Restriction map for
pFN21AB5941
(Circular) (Six-base) MAPSORT of: pFN21AB5941.seq Check: 1915 from: 1 to: 6229
pFN21AB5941 6229 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2995 2995
Size: 6229
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2531 2556 4039 278
Size: 53 83 186 936 995 25 1483 2468
Fragments arranged by size:
2468 1483 995 936 186 83 53 25
AccI GT'mk_AC
Cuts at: 1937 3572 1937
Size: 1635 4594
Acc65I G'GTAC_C
Cuts at: 3550 3550
Size: 6229
AclI AA'CG_TT
Cuts at: 1563 4356 4729 1563
Size: 2793 373 3063
Fragments arranged by size:
3063 2793 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2858 4292 5676 5944 1632
Size: 1226 1434 1384 268 1917
Fragments arranged by size:
1917 1434 1384 1226 268
AfeI AGC'GCT
Cuts at: 2212 3014 2212
Size: 802 5427
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6032
AflIII A'CryG_T
Cuts at: 1203 2343 5128 6105 1203
Size: 1140 2785 977 1327
Fragments arranged by size:
2785 1327 1140 977
AgeI A'CCGG_T
Cuts at: 5060 5060
Size: 6229
AhdI GACnn_n'nnGTC
Cuts at: 4958 4958
Size: 6229
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6229
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3235 1184
Size: 2051 4178
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2090 2122 2090
Size: 32 6197
AlwNI CAG_nnn'CTG
Cuts at: 3167 3494 3972 5544 3167
Size: 327 478 1572 3852
Fragments arranged by size:
3852 1572 478 327
ApaI G_GGCC'C
Cuts at: 2145 2225 3065 2145
Size: 80 840 5309
Fragments arranged by size:
5309 840 80
ApaLI G'TGCA_C
Cuts at: 4286 5442 4286
Size: 1156 5073
ApoI r'AATT_y
Cuts at: 1493 3538 3764 3916 6025 1493
Size: 2045 226 152 2109 1697
Fragments arranged by size:
2109 2045 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4783 160
Size: 872 3751 1606
Fragments arranged by size:
3751 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6229
AvaI C'yCGr_G
Cuts at: 1870 1943 2015 2136 2224 2252 2863 3554 1870
Size: 73 72 121 88 28 611 691 4545
Fragments arranged by size:
4545 691 611 121 88 73 72 28
BamHI G'GATC_C
Cuts at: 3559 3926 3559
Size: 367 5862
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2077 3550 5005 618
Size: 325 217 627 290 1473 1455 1842
Fragments arranged by size:
1842 1473 1455 627 325 290 217
BanII G_rGCy'C
Cuts at: 1391 2145 2225 2836 2870 2960 3065 3433 3548
Size: 754 80 611 34 90 105 368 115
Cuts at: 3548 1391
Size: 4072
Fragments arranged by size:
4072 754 611 368 115 105 90 80
34
BbeI G_GCGC'C
Cuts at: 2081 2081
Size: 6229
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5258
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2338 2372 4420 4454 1903
Size: 34 401 34 2048 34 3678
Fragments arranged by size:
3678 2048 401 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3094 4121 5331 3094
Size: 1027 1210 3992
Fragments arranged by size:
3992 1210 1027
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6229
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2075 2129 4840
Size: 107 122 71 631 605 403 54 2711
Cuts at: 4840 136
Size: 1525
Fragments arranged by size:
2711 1525 631 605 403 122 107 71
54
BglII A'GATC_T
Cuts at: 2847 6224 2847
Size: 3377 2852
BlpI GC'TnA_GC
Cuts at: 2624 2679 3495 3646 2624
Size: 55 816 151 5207
Fragments arranged by size:
5207 816 151 55
Bme1580I G_kGCm'C
Cuts at: 1790 2145 2225 2361 3065 3332 4290 5446 1790
Size: 355 80 136 704 267 958 1156 2573
Fragments arranged by size:
2573 1156 958 704 355 267 136 80
BmgBI CAC'GTC
Cuts at: 1318 2346 1318
Size: 1028 5201
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3141 3960 4918
Size: 416 53 83 399 24 1724 819 958
Cuts at: 4918 442
Size: 1753
Fragments arranged by size:
1753 1724 958 819 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6229
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3277 3277
Size: 6229
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2621 2722 3092 4889 1239
Size: 1382 101 370 1797 2579
Fragments arranged by size:
2579 1797 1382 370 101
Bpu10I CC'TnA_GC
Cuts at: 3333 3412 3333
Size: 79 6150
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4354 5219 5517 4354
Size: 865 298 5066
Fragments arranged by size:
5066 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4892 882
Size: 617 3393 2219
Fragments arranged by size:
3393 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2488 4020 493
Size: 711 1284 1532 2702
Fragments arranged by size:
2702 1532 1284 711
BsaBI GATnn'nnATC
Cuts at: 3105 6223 3105
Size: 3118 3111
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2021 2078 2442 2528
Size: 53 83 186 936 488 57 364 86
Cuts at: 2528 2553 2735 4036 4418 5053 275
Size: 25 182 1301 382 635 1451
Fragments arranged by size:
1451 1301 936 635 488 382 364 186
182 86 83 57 53 25
BsaWI w'CCGG_w
Cuts at: 4662 5060 5334 5481 5892 4662
Size: 398 274 147 411 4999
Fragments arranged by size:
4999 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2225 2255 2708 2738 3284
Size: 30 165 30 822 30 453 30 546
Cuts at: 3284 3314 1178
Size: 30 4093
Fragments arranged by size:
4093 822 546 453 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6229
BseYI C'CCAG_C
Cuts at: 2063 2213 3018 3245 3296 3303 3487 3509 5432
Size: 150 805 227 51 7 184 22 1923
Cuts at: 5432 5928 2063
Size: 496 2364
Fragments arranged by size:
2364 1923 805 496 227 184 150 51
22 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2267 2947 1163
Size: 573 531 680 4445
Fragments arranged by size:
4445 680 573 531
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2562 3679 4440 5468 1540
Size: 275 186 561 1117 761 1028 2301
Fragments arranged by size:
2301 1117 1028 761 561 275 186
BsiHKAI G_wGCw'C
Cuts at: 2436 2811 4290 4375 5446 2436
Size: 375 1479 85 1071 3219
Fragments arranged by size:
3219 1479 1071 375 85
BsmI GAATG_Cn'
Cuts at: 3740 3833 3740
Size: 93 6136
BsmBI CGTCTCn'nnnn_
Cuts at: 2535 2560 2535
Size: 25 6204
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2145 2225 2361 2436 2811 2836 2870
Size: 399 355 80 136 75 375 25 34
Cuts at: 2870 2960 3065 3332 3433 3548 4290 4375 5446
Size: 90 105 267 101 115 742 85 1071
Cuts at: 5446 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 375 355 267 136
115 105 101 90 85 80 75 34
25
BspEI T'CCGG_A
Cuts at: 5892 5892
Size: 6229
BspHI T'CATG_A
Cuts at: 4118 5848 4118
Size: 1730 4499
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2640 2995 3584 844
Size: 1796 355 589 3489
Fragments arranged by size:
3489 1796 589 355
BsrBI CCG'CTC
Cuts at: 1394 1823 2170 2481 4116 1394
Size: 429 347 311 1635 3507
Fragments arranged by size:
3507 1635 429 347 311
BsrDI GCAATG_nn'
Cuts at: 65 1248 4724 4898 65
Size: 1183 3476 174 1396
Fragments arranged by size:
3476 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2561 4873 5060 1700
Size: 110 751 2312 187 2869
Fragments arranged by size:
2869 2312 751 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6229
BssHII G'CGCG_C
Cuts at: 1925 2667 1925
Size: 742 5487
BssSI C'ACGA_G
Cuts at: 2301 2780 4289 5301 2301
Size: 479 1509 1012 3229
Fragments arranged by size:
3229 1509 1012 479
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6229
BstBI TT'CG_AA
Cuts at: 5033 5033
Size: 6229
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6229
BstXI CCAn_nnnn'nTGG
Cuts at: 2939 3517 2939
Size: 578 5651
BstYI r'GATC_y
Cuts at: 1972 2595 2847 3559 3926 4311 4328 5780 5866
Size: 623 252 712 367 385 17 1452 86
Cuts at: 5866 5878 6224 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 712 623 385 367 346 252
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2264 2295 2448 3192 3317 513
Size: 552 939 260 31 153 744 125 3425
Fragments arranged by size:
3425 939 744 552 260 153 125 31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5490
Fragments arranged by size:
5490 683 56
BtsI GCAGTG_nn'
Cuts at: 3042 3091 3181 3745 4539 4559 3042
Size: 49 90 564 794 20 4712
Fragments arranged by size:
4712 794 564 90 49 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6229
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6194
DraI TTT'AAA
Cuts at: 3533 3533
Size: 6229
DraIII CAC_nnn'GTG
Cuts at: 2491 2616 2491
Size: 125 6104
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5236 809
Size: 507 3920 1802
Fragments arranged by size:
3920 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2482 2559 3189
Size: 54 1425 50 275 18 652 77 630
Cuts at: 3189 3676 4565 6194 8
Size: 487 889 1629 43
Fragments arranged by size:
1629 1425 889 652 630 487 275 77
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2559 3676 1537
Size: 275 747 1117 4090
Fragments arranged by size:
4090 1117 747 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2142 2897 4159 1353
Size: 789 755 1262 3423
Fragments arranged by size:
3423 1262 789 755
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4816 5190 5336 387
Size: 1064 138 3227 374 146 1280
Fragments arranged by size:
3227 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2621 2722 2858 3092 4292 4889 5676
Size: 393 989 101 136 234 1200 597 787
Cuts at: 5676 5944 1239
Size: 268 1524
Fragments arranged by size:
1524 1200 989 787 597 393 268 234
136 101
EcoNI CCTnn'n_nnAGG
Cuts at: 2285 2285
Size: 6229
EcoO109I rG'GnC_Cy
Cuts at: 2141 2980 3061 3062 3154 3164 2141
Size: 839 81 1 92 10 5206
Fragments arranged by size:
5206 839 92 81 10 1
EcoRI G'AATT_C
Cuts at: 3538 3916 3538
Size: 378 5851
EcoRV GAT'ATC
Cuts at: 2402 2402
Size: 6229
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2929 2929
Size: 6229
FspI TGC'GCA
Cuts at: 4735 4735
Size: 6229
HaeII r_GCGC'y
Cuts at: 1678 2081 2214 2394 2709 3016 5376 1678
Size: 403 133 180 315 307 2360 2531
Fragments arranged by size:
2531 2360 403 315 307 180 133
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2708 2740 3188 3220 3284
Size: 32 135 32 1136 32 448 32 64
Cuts at: 3284 3316 3395 3427 4870 4902 4944 4976 1373
Size: 32 79 32 1443 32 42 32 2626
Fragments arranged by size:
2626 1443 1136 448 135 79 64 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3573 3819 669
Size: 988 281 1635 246 3079
Fragments arranged by size:
3079 1635 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6229
HpaI GTT'AAC
Cuts at: 3819 3819
Size: 6229
KasI G'GCGC_C
Cuts at: 2077 2077
Size: 6229
KpnI G_GTAC'C
Cuts at: 3554 3554
Size: 6229
MfeI C'AATT_G
Cuts at: 3828 3828
Size: 6229
MluI A'CGCG_T
Cuts at: 6105 6105
Size: 6229
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2154 2694 3486 5343 5527 1305
Size: 849 540 792 1857 184 2007
Fragments arranged by size:
2007 1857 849 792 540 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3191 10
Size: 54 1425 343 1359 3048
Fragments arranged by size:
3048 1425 1359 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2421 2780 2792 4187 4546 4705 518
Size: 648 1255 359 12 1395 359 159 2042
Fragments arranged by size:
2042 1395 1255 648 359 359 159 12
MspA1I CmG'CkG
Cuts at: 1606 2920 3494 3645 4322 5470 5715 1606
Size: 1314 574 151 677 1148 245 2120
Fragments arranged by size:
2120 1314 1148 677 574 245 151
NaeI GCC'GGC
Cuts at: 2563 2563
Size: 6229
NarI GG'CG_CC
Cuts at: 2078 2078
Size: 6229
NcoI C'CATG_G
Cuts at: 513 1065 2004 2264 3192 3317 513
Size: 552 939 260 928 125 3425
Fragments arranged by size:
3425 939 928 552 260 125
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6229
NgoMIV G'CCGG_C
Cuts at: 2561 2561
Size: 6229
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6229
NotI GC'GGCC_GC
Cuts at: 3676 3676
Size: 6229
NspI r_CATG'y
Cuts at: 1132 1229 3095 3587 5132 1132
Size: 97 1866 492 1545 2229
Fragments arranged by size:
2229 1866 1545 492 97
PasI CC'CwG_GG
Cuts at: 1348 3161 3424 1348
Size: 1813 263 4153
Fragments arranged by size:
4153 1813 263
PciI A'CATG_T
Cuts at: 5128 5128
Size: 6229
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3167 1108
Size: 2059 4170
PfoI T'CCnGG_A
Cuts at: 2094 2598 3288 2094
Size: 504 690 5035
Fragments arranged by size:
5035 690 504
PmeI GTTT'AAAC
Cuts at: 3533 3533
Size: 6229
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4298 4330 5811 5843 4298
Size: 32 1481 32 4684
Fragments arranged by size:
4684 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2980 3154 2980
Size: 174 6055
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6229
PsiI TTA'TAA
Cuts at: 3799 3799
Size: 6229
PspOMI G'GGCC_C
Cuts at: 2141 2221 3061 2141
Size: 80 840 5309
Fragments arranged by size:
5309 840 80
PspXI vC'TCGA_Gb
Cuts at: 2252 2252
Size: 6229
PstI C_TGCA'G
Cuts at: 830 3581 830
Size: 2751 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6229
PvuII CAG'CTG
Cuts at: 2920 2920
Size: 6229
SalI G'TCGA_C
Cuts at: 1936 3571 1936
Size: 1635 4594
SapI GCTCTTCn'nnn_
Cuts at: 2142 2142
Size: 6229
SbfI CC_TGCA'GG
Cuts at: 3581 3581
Size: 6229
ScaI AGT'ACT
Cuts at: 4477 6189 4477
Size: 1712 4517
SfcI C'TryA_G
Cuts at: 826 1045 2233 2334 3406 3577 4712 5393 5584
Size: 219 1188 101 1072 171 1135 681 191
Cuts at: 5584 826
Size: 1471
Fragments arranged by size:
1471 1188 1135 1072 681 219 191 171
101
SfoI GGC'GCC
Cuts at: 2079 2079
Size: 6229
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6229
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6229
SmaI CCC'GGG
Cuts at: 1872 2138 2226 3556 1872
Size: 266 88 1330 4545
Fragments arranged by size:
4545 1330 266 88
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2252 4333 5234 5496 820
Size: 197 926 309 2081 901 262 1553
Fragments arranged by size:
2081 1553 926 901 309 262 197
SnaBI TAC'GTA
Cuts at: 493 4020 493
Size: 3527 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6229
SphI G_CATG'C
Cuts at: 1132 3095 3587 1132
Size: 1963 492 3774
Fragments arranged by size:
3774 1963 492
SrfI GCCC'GGGC
Cuts at: 2226 2226
Size: 6229
SspI AAT'ATT
Cuts at: 5 52 4153 5
Size: 47 4101 2081
Fragments arranged by size:
4101 2081 47
StuI AGG'CCT
Cuts at: 1517 2889 1517
Size: 1372 4857
StyI C'CwwG_G
Cuts at: 513 1065 2004 2264 3192 3317 3433 3668 513
Size: 552 939 260 928 125 116 235 3074
Fragments arranged by size:
3074 939 928 552 260 235 125 116
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2536 2915 4256 4273 4426 4611 5056
Size: 1255 956 379 1341 17 153 185 445
Cuts at: 5056 325
Size: 1498
Fragments arranged by size:
1498 1341 1255 956 445 379 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3279 4475 6187
Size: 274 80 33 51 1443 1302 1196 1712
Cuts at: 6187 96
Size: 138
Fragments arranged by size:
1712 1443 1302 1196 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2171 2203 6189 6221 2171
Size: 32 3986 32 2179
Fragments arranged by size:
3986 2179 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6229
XbaI T'CTAG_A
Cuts at: 3565 3565
Size: 6229
XhoI C'TCGA_G
Cuts at: 1943 2252 1943
Size: 309 5920
XmaI C'CCGG_G
Cuts at: 1870 2136 2224 3554 1870
Size: 266 88 1330 4545
Fragments arranged by size:
4545 1330 266 88
XmnI GAAnn'nnTTC
Cuts at: 2151 4358 2151
Size: 2207 4022
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2529 2554 4037 276
Size: 53 83 186 936 995 25 1483 2468
Fragments arranged by size:
2468 1483 995 936 186 83 53 25
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I Bsu36I
EcoICRI FseI FspAI NruI NsiI PacI PmlI PsrI
RsrII SacI SacII SanDI SexAI SfiI SwaI XcmI