Restriction map for
pFN21AB5932
(Circular) (Six-base) MAPSORT of: pFN21AB5932.seq Check: 9816 from: 1 to: 6319
pFN21AB5932 6319 bp
With 182 enzymes: SgfI *
August 16, 2010 14:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2296 2296
Size: 6319
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4129 278
Size: 53 83 186 936 2593 2468
Fragments arranged by size:
2593 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3662 1937
Size: 1725 4594
Acc65I G'GTAC_C
Cuts at: 3212 3640 3212
Size: 428 5891
AclI AA'CG_TT
Cuts at: 1563 2892 4446 4819 1563
Size: 1329 1554 373 3063
Fragments arranged by size:
3063 1554 1329 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2372 3226 3456 4382 5766 6034 1632
Size: 740 854 230 926 1384 268 1917
Fragments arranged by size:
1917 1384 926 854 740 268 230
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6122
AflIII A'CryG_T
Cuts at: 1203 5218 6195 1203
Size: 4015 977 1327
Fragments arranged by size:
4015 1327 977
AgeI A'CCGG_T
Cuts at: 5150 5150
Size: 6319
AhdI GACnn_n'nnGTC
Cuts at: 2692 5048 2692
Size: 2356 3963
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6319
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2579 1184
Size: 1395 4924
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3086 3118 3086
Size: 32 6287
AlwNI CAG_nnn'CTG
Cuts at: 2362 4062 5634 2362
Size: 1700 1572 3047
Fragments arranged by size:
3047 1700 1572
ApaLI G'TGCA_C
Cuts at: 4376 5532 4376
Size: 1156 5163
ApoI r'AATT_y
Cuts at: 1493 3628 3854 4006 6115 1493
Size: 2135 226 152 2109 1697
Fragments arranged by size:
2135 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4873 160
Size: 872 3841 1606
Fragments arranged by size:
3841 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6319
AvaI C'yCGr_G
Cuts at: 1870 1943 2233 2300 2312 2910 3644 1870
Size: 73 290 67 12 598 734 4545
Fragments arranged by size:
4545 734 598 290 73 67 12
BamHI G'GATC_C
Cuts at: 2615 3649 4016 2615
Size: 1034 367 4918
Fragments arranged by size:
4918 1034 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2216 2229 2308 3001 3212
Size: 325 217 627 429 13 79 693 211
Cuts at: 3212 3419 3640 5095 618
Size: 207 221 1455 1842
Fragments arranged by size:
1842 1455 693 627 429 325 221 217
211 207 79 13
BanII G_rGCy'C
Cuts at: 1391 3638 1391
Size: 2247 4072
BbeI G_GCGC'C
Cuts at: 2220 2220
Size: 6319
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3462 928
Size: 971 1563 3785
Fragments arranged by size:
3785 1563 971
BbvCI CC'TCA_GC
Cuts at: 2922 2922
Size: 6319
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4510 4544 1903
Size: 34 2573 34 3678
Fragments arranged by size:
3678 2573 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2635 4211 5421 2635
Size: 1576 1210 3533
Fragments arranged by size:
3533 1576 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6319
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4930 136
Size: 107 122 71 631 605 3258 1525
Fragments arranged by size:
3258 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2427 6314 2427
Size: 3887 2432
BlpI GC'TnA_GC
Cuts at: 2820 3736 2820
Size: 916 5403
Bme1580I G_kGCm'C
Cuts at: 1790 2232 2313 4380 5536 1790
Size: 442 81 2067 1156 2573
Fragments arranged by size:
2573 2067 1156 442 81
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6319
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2615 3104 3614
Size: 416 53 83 399 24 1198 489 510
Cuts at: 3614 4050 5008 442
Size: 436 958 1753
Fragments arranged by size:
1753 1198 958 510 489 436 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6319
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2338 2338
Size: 6319
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2796 4979 1239
Size: 1557 2183 2579
Fragments arranged by size:
2579 2183 1557
Bpu10I CC'TnA_GC
Cuts at: 2922 2922
Size: 6319
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2146 4444 5309 5607 2146
Size: 2298 865 298 2858
Fragments arranged by size:
2858 2298 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2715 3245 4982 882
Size: 617 1216 530 1737 2219
Fragments arranged by size:
2219 1737 1216 617 530
BsaAI yAC'GTr
Cuts at: 493 1204 2470 4110 493
Size: 711 1266 1640 2702
Fragments arranged by size:
2702 1640 1266 711
BsaBI GATnn'nnATC
Cuts at: 2381 2411 6313 2381
Size: 30 3902 2387
Fragments arranged by size:
3902 2387 30
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2136 2217 2478 2514
Size: 53 83 186 936 603 81 261 36
Cuts at: 2514 4126 4508 5143 275
Size: 1612 382 635 1451
Fragments arranged by size:
1612 1451 936 635 603 382 261 186
83 81 53 36
BsaWI w'CCGG_w
Cuts at: 2724 4752 5150 5424 5571 5982 2724
Size: 2028 398 274 147 411 3061
Fragments arranged by size:
3061 2028 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6094
Fragments arranged by size:
6094 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2171 1186
Size: 985 5334
BseYI C'CCAG_C
Cuts at: 2033 2225 2362 2371 2938 2957 5522 6018 2033
Size: 192 137 9 567 19 2565 496 2334
Fragments arranged by size:
2565 2334 567 496 192 137 19 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2555 1163
Size: 573 819 4927
Fragments arranged by size:
4927 819 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2059 3769 4530 5558 1540
Size: 275 186 58 1710 761 1028 2301
Fragments arranged by size:
2301 1710 1028 761 275 186 58
BsiHKAI G_wGCw'C
Cuts at: 4380 4465 5536 4380
Size: 85 1071 5163
Fragments arranged by size:
5163 1071 85
BsmI GAATG_Cn'
Cuts at: 3830 3923 3830
Size: 93 6226
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2232 2313 3638 4380 4465 5536 1391
Size: 399 442 81 1325 742 85 1071 2174
Fragments arranged by size:
2174 1325 1071 742 442 399 85 81
BspEI T'CCGG_A
Cuts at: 5982 5982
Size: 6319
BspHI T'CATG_A
Cuts at: 4208 5938 4208
Size: 1730 4589
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2296 2304 2514 2910 3674 844
Size: 1452 8 210 396 764 3489
Fragments arranged by size:
3489 1452 764 396 210 8
BsrBI CCG'CTC
Cuts at: 1394 1823 2073 4206 1394
Size: 429 250 2133 3507
Fragments arranged by size:
3507 2133 429 250
BsrDI GCAATG_nn'
Cuts at: 65 1248 2952 3318 4814 4988 65
Size: 1183 1704 366 1496 174 1396
Fragments arranged by size:
1704 1496 1396 1183 366 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2038 4963 5150 1700
Size: 110 228 2925 187 2869
Fragments arranged by size:
2925 2869 228 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6319
BssHII G'CGCG_C
Cuts at: 1925 2247 2548 1925
Size: 322 301 5696
Fragments arranged by size:
5696 322 301
BssSI C'ACGA_G
Cuts at: 4379 5391 4379
Size: 1012 5307
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3339 794
Size: 2545 3774
BstBI TT'CG_AA
Cuts at: 5123 5123
Size: 6319
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6319
BstXI CCAn_nnnn'nTGG
Cuts at: 2336 3142 2336
Size: 806 5513
BstYI r'GATC_y
Cuts at: 1972 2427 2615 3187 3649 4016 4401 4418 5870
Size: 455 188 572 462 367 385 17 1452
Cuts at: 5870 5956 5968 6314 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 572 462 455 385 367 346
188 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2837 2837
Size: 6319
BtgI C'CryG_G
Cuts at: 513 1065 2004 2856 3083 513
Size: 552 939 852 227 3749
Fragments arranged by size:
3749 939 852 552 227
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2682 487
Size: 683 56 1456 4124
Fragments arranged by size:
4124 1456 683 56
BtsI GCAGTG_nn'
Cuts at: 3333 3835 4629 4649 3333
Size: 502 794 20 5003
Fragments arranged by size:
5003 794 502 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6319
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6284
DraI TTT'AAA
Cuts at: 3623 3623
Size: 6319
DraIII CAC_nnn'GTG
Cuts at: 2830 2830
Size: 6319
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5326 809
Size: 507 4010 1802
Fragments arranged by size:
4010 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2056 2775 3615
Size: 54 1425 50 275 18 226 719 840
Cuts at: 3615 3766 4655 6284 8
Size: 151 889 1629 43
Fragments arranged by size:
1629 1425 889 840 719 275 226 151
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2056 3766 1537
Size: 275 244 1710 4090
Fragments arranged by size:
4090 1710 275 244
EarI CTCTTCn'nnn_
Cuts at: 1353 3132 3245 4249 1353
Size: 1779 113 1004 3423
Fragments arranged by size:
3423 1779 1004 113
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2462 2640 4906 5280 5426 387
Size: 1064 138 873 178 2266 374 146 1280
Fragments arranged by size:
2266 1280 1064 873 374 178 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2372 2796 3226 3456 4382 4979 5766
Size: 393 740 424 430 230 926 597 787
Cuts at: 5766 6034 1239
Size: 268 1524
Fragments arranged by size:
1524 926 787 740 597 430 424 393
268 230
EcoNI CCTnn'n_nnAGG
Cuts at: 2667 2667
Size: 6319
EcoO109I rG'GnC_Cy
Cuts at: 3262 3262
Size: 6319
EcoRI G'AATT_C
Cuts at: 3628 4006 3628
Size: 378 5941
FspI TGC'GCA
Cuts at: 4825 4825
Size: 6319
HaeII r_GCGC'y
Cuts at: 1678 2166 2220 5466 1678
Size: 488 54 3246 2531
Fragments arranged by size:
3246 2531 488 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2678 2710 2962 2994 4960
Size: 32 135 32 1106 32 252 32 1966
Cuts at: 4960 4992 5034 5066 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1966 1106 252 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3663 3909 669
Size: 988 281 1725 246 3079
Fragments arranged by size:
3079 1725 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6319
HpaI GTT'AAC
Cuts at: 3909 3909
Size: 6319
KasI G'GCGC_C
Cuts at: 2216 2216
Size: 6319
KpnI G_GTAC'C
Cuts at: 3216 3644 3216
Size: 428 5891
MfeI C'AATT_G
Cuts at: 3558 3918 3558
Size: 360 5959
MluI A'CGCG_T
Cuts at: 6195 6195
Size: 6319
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2913 5433 5617 1305
Size: 1608 2520 184 2007
Fragments arranged by size:
2520 2007 1608 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4497
Fragments arranged by size:
4497 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4277 4636 4795 518
Size: 648 3111 359 159 2042
Fragments arranged by size:
3111 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2068 2938 3735 4412 5560 5805 1606
Size: 462 870 797 677 1148 245 2120
Fragments arranged by size:
2120 1148 870 797 677 462 245
NaeI GCC'GGC
Cuts at: 2040 2040
Size: 6319
NarI GG'CG_CC
Cuts at: 2217 2217
Size: 6319
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4828
Fragments arranged by size:
4828 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6319
NgoMIV G'CCGG_C
Cuts at: 2038 2038
Size: 6319
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6319
NotI GC'GGCC_GC
Cuts at: 3766 3766
Size: 6319
NspI r_CATG'y
Cuts at: 1132 1229 2587 3082 3677 5222 1132
Size: 97 1358 495 595 1545 2229
Fragments arranged by size:
2229 1545 1358 595 495 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6319
PciI A'CATG_T
Cuts at: 5218 5218
Size: 6319
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6319
PfoI T'CCnGG_A
Cuts at: 3157 3157
Size: 6319
PmeI GTTT'AAAC
Cuts at: 3623 3623
Size: 6319
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4388 4420 5901 5933 4388
Size: 32 1481 32 4774
Fragments arranged by size:
4774 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3262 3262
Size: 6319
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6319
PsiI TTA'TAA
Cuts at: 3889 3889
Size: 6319
PstI C_TGCA'G
Cuts at: 830 2213 3671 830
Size: 1383 1458 3478
Fragments arranged by size:
3478 1458 1383
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6319
PvuII CAG'CTG
Cuts at: 2938 2938
Size: 6319
SalI G'TCGA_C
Cuts at: 1936 3661 1936
Size: 1725 4594
SbfI CC_TGCA'GG
Cuts at: 3671 3671
Size: 6319
ScaI AGT'ACT
Cuts at: 4567 6279 4567
Size: 1712 4607
SfcI C'TryA_G
Cuts at: 826 1045 2209 3667 4802 5483 5674 826
Size: 219 1164 1458 1135 681 191 1471
Fragments arranged by size:
1471 1458 1164 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2218 2218
Size: 6319
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6319
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6319
SmaI CCC'GGG
Cuts at: 1872 2235 3646 1872
Size: 363 1411 4545
Fragments arranged by size:
4545 1411 363
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2125 4423 5324 5586 820
Size: 197 926 182 2298 901 262 1553
Fragments arranged by size:
2298 1553 926 901 262 197 182
SnaBI TAC'GTA
Cuts at: 493 4110 493
Size: 3617 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6319
SphI G_CATG'C
Cuts at: 1132 3082 3677 1132
Size: 1950 595 3774
Fragments arranged by size:
3774 1950 595
SspI AAT'ATT
Cuts at: 5 52 4243 5
Size: 47 4191 2081
Fragments arranged by size:
4191 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6319
StyI C'CwwG_G
Cuts at: 513 1065 2004 2772 3758 513
Size: 552 939 768 986 3074
Fragments arranged by size:
3074 986 939 768 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2534 2625 4346 4363 4516 4701 5146
Size: 1255 954 91 1721 17 153 185 445
Cuts at: 5146 325
Size: 1498
Fragments arranged by size:
1721 1498 1255 954 445 185 153 91
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2580 3289 4565
Size: 274 80 33 51 1443 603 709 1276
Cuts at: 4565 6277 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1276 709 603 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6279 6311 6279
Size: 32 6287
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6319
XbaI T'CTAG_A
Cuts at: 3655 3655
Size: 6319
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6319
XmaI C'CCGG_G
Cuts at: 1870 2233 3644 1870
Size: 363 1411 4545
Fragments arranged by size:
4545 1411 363
XmnI GAAnn'nnTTC
Cuts at: 4448 4448
Size: 6319
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4127 276
Size: 53 83 186 936 2593 2468
Fragments arranged by size:
2593 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I EcoICRI EcoRV FalI FseI FspAI NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SrfI SwaI XcmI