Restriction map for
pFN21AB5923
(Circular) (Six-base) MAPSORT of: pFN21AB5923.seq Check: 8720 from: 1 to: 6220
pFN21AB5923 6220 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4030 278
Size: 53 83 186 936 2494 2468
Fragments arranged by size:
2494 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3377 3563 1937
Size: 1440 186 4594
Fragments arranged by size:
4594 1440 186
Acc65I G'GTAC_C
Cuts at: 3416 3541 3416
Size: 125 6095
AclI AA'CG_TT
Cuts at: 1563 4347 4720 1563
Size: 2784 373 3063
Fragments arranged by size:
3063 2784 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2552 2906 4283 5667 5935 1632
Size: 920 354 1377 1384 268 1917
Fragments arranged by size:
1917 1384 1377 920 354 268
AflII C'TTAA_G
Cuts at: 820 1017 3167 820
Size: 197 2150 3873
Fragments arranged by size:
3873 2150 197
AflIII A'CryG_T
Cuts at: 1203 5119 6096 1203
Size: 3916 977 1327
Fragments arranged by size:
3916 1327 977
AgeI A'CCGG_T
Cuts at: 5051 5051
Size: 6220
AhdI GACnn_n'nnGTC
Cuts at: 4949 4949
Size: 6220
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6220
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3011 1184
Size: 1827 4393
AlwNI CAG_nnn'CTG
Cuts at: 3963 5535 3963
Size: 1572 4648
ApaI G_GGCC'C
Cuts at: 3412 3412
Size: 6220
ApaLI G'TGCA_C
Cuts at: 2787 4277 5433 2787
Size: 1490 1156 3574
Fragments arranged by size:
3574 1490 1156
ApoI r'AATT_y
Cuts at: 1493 2963 3529 3755 3907 6016 1493
Size: 1470 566 226 152 2109 1697
Fragments arranged by size:
2109 1697 1470 566 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4774 160
Size: 872 3742 1606
Fragments arranged by size:
3742 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6220
AvaI C'yCGr_G
Cuts at: 1870 1943 2193 2234 2748 2886 3117 3144 3411
Size: 73 250 41 514 138 231 27 267
Cuts at: 3411 3423 3545 1870
Size: 12 122 4545
Fragments arranged by size:
4545 514 267 250 231 138 122 73
41 27 12
AvrII C'CTAG_G
Cuts at: 2423 2423
Size: 6220
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2403 2436 3215 3248 2403
Size: 33 779 33 5375
Fragments arranged by size:
5375 779 33 33
BamHI G'GATC_C
Cuts at: 3550 3917 3550
Size: 367 5853
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2104 3416 3541 4996 618
Size: 325 217 627 317 1312 125 1455 1842
Fragments arranged by size:
1842 1455 1312 627 325 317 217 125
BanII G_rGCy'C
Cuts at: 1391 2048 2141 2234 3412 3539 1391
Size: 657 93 93 1178 127 4072
Fragments arranged by size:
4072 1178 657 127 93 93
BbeI G_GCGC'C
Cuts at: 2108 2108
Size: 6220
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2658 2912 3475 928
Size: 971 759 254 563 3673
Fragments arranged by size:
3673 971 759 563 254
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2203 2237 4411 4445 1903
Size: 34 50 34 182 34 2174 34 3678
Fragments arranged by size:
3678 2174 182 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4112 5322 4112
Size: 1210 5010
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6220
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2102 4831 136
Size: 107 122 71 631 605 430 2729 1525
Fragments arranged by size:
2729 1525 631 605 430 122 107 71
BglII A'GATC_T
Cuts at: 6215 6215
Size: 6220
BlpI GC'TnA_GC
Cuts at: 2228 3637 2228
Size: 1409 4811
Bme1580I G_kGCm'C
Cuts at: 1790 2791 3412 4281 5437 1790
Size: 1001 621 869 1156 2573
Fragments arranged by size:
2573 1156 1001 869 621
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6220
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2473 3951 4909
Size: 416 53 83 399 24 1056 1478 958
Cuts at: 4909 442
Size: 1753
Fragments arranged by size:
1753 1478 1056 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6220
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2106 2191 2332 3092 4880 1239
Size: 867 85 141 760 1788 2579
Fragments arranged by size:
2579 1788 867 760 141 85
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2996 4345 5210 5508 2996
Size: 1349 865 298 3708
Fragments arranged by size:
3708 1349 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3150 3420 4883 882
Size: 617 1651 270 1463 2219
Fragments arranged by size:
2219 1651 1463 617 270
BsaAI yAC'GTr
Cuts at: 493 1204 2824 4011 493
Size: 711 1620 1187 2702
Fragments arranged by size:
2702 1620 1187 711
BsaBI GATnn'nnATC
Cuts at: 6214 6214
Size: 6220
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2105 2171 3439 4027
Size: 53 83 186 936 572 66 1268 588
Cuts at: 4027 4409 5044 275
Size: 382 635 1451
Fragments arranged by size:
1451 1268 936 635 588 572 382 186
83 66 53
BsaWI w'CCGG_w
Cuts at: 2859 4653 5051 5325 5472 5883 2859
Size: 1794 398 274 147 411 3196
Fragments arranged by size:
3196 1794 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5995
Fragments arranged by size:
5995 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2419 1186
Size: 1233 4987
BseYI C'CCAG_C
Cuts at: 2153 3007 3048 3457 5423 5919 2153
Size: 854 41 409 1966 496 2454
Fragments arranged by size:
2454 1966 854 496 409 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5647
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2028 3670 4431 5459 1540
Size: 275 186 27 1642 761 1028 2301
Fragments arranged by size:
2301 1642 1028 761 275 186 27
BsiHKAI G_wGCw'C
Cuts at: 2048 2318 2791 3255 4281 4366 5437 2048
Size: 270 473 464 1026 85 1071 2831
Fragments arranged by size:
2831 1071 1026 473 464 270 85
BsmI GAATG_Cn'
Cuts at: 2722 3731 3824 2722
Size: 1009 93 5118
Fragments arranged by size:
5118 1009 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3446 3446
Size: 6220
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2048 2141 2234 2318 2791 3255 3412
Size: 399 258 93 93 84 473 464 157
Cuts at: 3412 3539 4281 4366 5437 1391
Size: 127 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 473 464 399 258 157
127 93 93 85 84
BspEI T'CCGG_A
Cuts at: 2859 5883 2859
Size: 3024 3196
BspHI T'CATG_A
Cuts at: 4109 5839 4109
Size: 1730 4490
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2094 2814 3363 3575 844
Size: 1250 720 549 212 3489
Fragments arranged by size:
3489 1250 720 549 212
BsrBI CCG'CTC
Cuts at: 1394 1823 2120 2160 2755 4107 1394
Size: 429 297 40 595 1352 3507
Fragments arranged by size:
3507 1352 595 429 297 40
BsrDI GCAATG_nn'
Cuts at: 65 1248 4715 4889 65
Size: 1183 3467 174 1396
Fragments arranged by size:
3467 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3044 4864 5051 1700
Size: 110 1234 1820 187 2869
Fragments arranged by size:
2869 1820 1234 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6220
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6220
BssSI C'ACGA_G
Cuts at: 2687 4280 5292 2687
Size: 1593 1012 3615
Fragments arranged by size:
3615 1593 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6220
BstBI TT'CG_AA
Cuts at: 5024 5024
Size: 6220
BstEII G'GTnAC_C
Cuts at: 1184 3139 1184
Size: 1955 4265
BstXI CCAn_nnnn'nTGG
Cuts at: 3007 3007
Size: 6220
BstYI r'GATC_y
Cuts at: 1972 3076 3550 3917 4302 4319 5771 5857 5869
Size: 1104 474 367 385 17 1452 86 12
Cuts at: 5869 6215 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1104 474 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 3378 3378
Size: 6220
Bsu36I CC'TnA_GG
Cuts at: 3219 3219
Size: 6220
BtgI C'CryG_G
Cuts at: 513 1065 2028 3430 513
Size: 552 963 1402 3303
Fragments arranged by size:
3303 1402 963 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2510 487
Size: 683 56 1284 4197
Fragments arranged by size:
4197 1284 683 56
BtsI GCAGTG_nn'
Cuts at: 2965 3253 3736 4530 4550 2965
Size: 288 483 794 20 4635
Fragments arranged by size:
4635 794 483 288 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6220
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6185
DraI TTT'AAA
Cuts at: 3524 3524
Size: 6220
DraIII CAC_nnn'GTG
Cuts at: 2041 2770 2041
Size: 729 5491
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3479 5227 809
Size: 507 2163 1748 1802
Fragments arranged by size:
2163 1802 1748 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 2761 2826
Size: 54 1425 50 275 18 195 736 65
Cuts at: 2826 3126 3667 4556 6185 8
Size: 300 541 889 1629 43
Fragments arranged by size:
1629 1425 889 736 541 300 275 195
65 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2025 3667 1537
Size: 275 213 1642 4090
Fragments arranged by size:
4090 1642 275 213
EarI CTCTTCn'nnn_
Cuts at: 1353 4150 1353
Size: 2797 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2049 2508 4807 5181 5327 387
Size: 1064 138 460 459 2299 374 146 1280
Fragments arranged by size:
2299 1280 1064 460 459 374 146 138
EcoICRI GAG'CTC
Cuts at: 2046 2046
Size: 6220
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2106 2191 2332 2552 2906 3092 4283
Size: 393 474 85 141 220 354 186 1191
Cuts at: 4283 4880 5667 5935 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1191 787 597 474 393 354 268
220 186 141 85
EcoNI CCTnn'n_nnAGG
Cuts at: 2436 2436
Size: 6220
EcoO109I rG'GnC_Cy
Cuts at: 2467 2551 2572 2613 3408 2467
Size: 84 21 41 795 5279
Fragments arranged by size:
5279 795 84 41 21
EcoRI G'AATT_C
Cuts at: 2963 3529 3907 2963
Size: 566 378 5276
Fragments arranged by size:
5276 566 378
FspI TGC'GCA
Cuts at: 4726 4726
Size: 6220
HaeII r_GCGC'y
Cuts at: 1678 2058 2108 2132 2914 5367 1678
Size: 380 50 24 782 2453 2531
Fragments arranged by size:
2531 2453 782 380 50 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3461 3493 4861 4893 4935
Size: 32 135 32 1889 32 1368 32 42
Cuts at: 4935 4967 1373
Size: 32 2626
Fragments arranged by size:
2626 1889 1368 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3564 3810 669
Size: 988 281 1626 246 3079
Fragments arranged by size:
3079 1626 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6220
HpaI GTT'AAC
Cuts at: 3810 3810
Size: 6220
KasI G'GCGC_C
Cuts at: 2104 2104
Size: 6220
KpnI G_GTAC'C
Cuts at: 3420 3545 3420
Size: 125 6095
MfeI C'AATT_G
Cuts at: 3819 3819
Size: 6220
MluI A'CGCG_T
Cuts at: 6096 6096
Size: 6220
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2381 5334 5518 1305
Size: 1076 2953 184 2007
Fragments arranged by size:
2953 2007 1076 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2763 2828 3128 10
Size: 54 1425 343 931 65 300 3102
Fragments arranged by size:
3102 1425 931 343 300 65 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2879 3137 4178 4537 4696 518
Size: 648 1713 258 1041 359 159 2042
Fragments arranged by size:
2042 1713 1041 648 359 258 159
MspA1I CmG'CkG
Cuts at: 1606 2227 2926 3636 4313 5461 5706 1606
Size: 621 699 710 677 1148 245 2120
Fragments arranged by size:
2120 1148 710 699 677 621 245
NarI GG'CG_CC
Cuts at: 2105 2105
Size: 6220
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5668
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6220
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6220
NotI GC'GGCC_GC
Cuts at: 3667 3667
Size: 6220
NspI r_CATG'y
Cuts at: 1132 1229 2486 3578 5123 1132
Size: 97 1257 1092 1545 2229
Fragments arranged by size:
2229 1545 1257 1092 97
PasI CC'CwG_GG
Cuts at: 1348 3496 1348
Size: 2148 4072
PciI A'CATG_T
Cuts at: 5119 5119
Size: 6220
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2731 2770 3397 1108
Size: 1623 39 627 3931
Fragments arranged by size:
3931 1623 627 39
PmeI GTTT'AAAC
Cuts at: 3524 3524
Size: 6220
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4289 4321 5802 5834 4289
Size: 32 1481 32 4675
Fragments arranged by size:
4675 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2551 2572 2613 2551
Size: 21 41 6158
Fragments arranged by size:
6158 41 21
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6220
PsiI TTA'TAA
Cuts at: 3790 3790
Size: 6220
PspOMI G'GGCC_C
Cuts at: 3408 3408
Size: 6220
PspXI vC'TCGA_Gb
Cuts at: 2193 2193
Size: 6220
PstI C_TGCA'G
Cuts at: 830 2226 3179 3572 830
Size: 1396 953 393 3478
Fragments arranged by size:
3478 1396 953 393
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6220
PvuII CAG'CTG
Cuts at: 2227 2926 2227
Size: 699 5521
SacI G_AGCT'C
Cuts at: 2048 2048
Size: 6220
SalI G'TCGA_C
Cuts at: 1936 3562 1936
Size: 1626 4594
SanDI GG'GwC_CC
Cuts at: 2613 2613
Size: 6220
SbfI CC_TGCA'GG
Cuts at: 3572 3572
Size: 6220
ScaI AGT'ACT
Cuts at: 4468 6180 4468
Size: 1712 4508
SexAI A'CCwGG_T
Cuts at: 2037 2553 2037
Size: 516 5704
SfcI C'TryA_G
Cuts at: 826 1045 2222 3175 3568 4703 5384 5575 826
Size: 219 1177 953 393 1135 681 191 1471
Fragments arranged by size:
1471 1177 1135 953 681 393 219 191
SfoI GGC'GCC
Cuts at: 2106 2106
Size: 6220
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6220
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6220
SmaI CCC'GGG
Cuts at: 1872 2236 2750 2888 3413 3547 1872
Size: 364 514 138 525 134 4545
Fragments arranged by size:
4545 525 514 364 138 134
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2193 2975 3167 4324 5225 5487
Size: 197 926 250 782 192 1157 901 262
Cuts at: 5487 820
Size: 1553
Fragments arranged by size:
1553 1157 926 901 782 262 250 197
192
SnaBI TAC'GTA
Cuts at: 493 4011 493
Size: 3518 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6220
SphI G_CATG'C
Cuts at: 1132 2486 3578 1132
Size: 1354 1092 3774
Fragments arranged by size:
3774 1354 1092
SspI AAT'ATT
Cuts at: 5 52 4144 5
Size: 47 4092 2081
Fragments arranged by size:
4092 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6220
StyI C'CwwG_G
Cuts at: 513 1065 2423 3000 3659 513
Size: 552 1358 577 659 3074
Fragments arranged by size:
3074 1358 659 577 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2227 2984 4247 4264 4417 4602 5047
Size: 1255 647 757 1263 17 153 185 445
Cuts at: 5047 325
Size: 1498
Fragments arranged by size:
1498 1263 1255 757 647 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4466 6178 96
Size: 274 80 33 51 1443 2489 1712 138
Fragments arranged by size:
2489 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6180 6212 6180
Size: 32 6188
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6220
XbaI T'CTAG_A
Cuts at: 3556 3556
Size: 6220
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3454 3454
Size: 6220
XhoI C'TCGA_G
Cuts at: 1943 2193 1943
Size: 250 5970
XmaI C'CCGG_G
Cuts at: 1870 2234 2748 2886 3411 3545 1870
Size: 364 514 138 525 134 4545
Fragments arranged by size:
4545 525 514 364 138 134
XmnI GAAnn'nnTTC
Cuts at: 2720 2874 4349 2720
Size: 154 1475 4591
Fragments arranged by size:
4591 1475 154
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4028 276
Size: 53 83 186 936 2494 2468
Fragments arranged by size:
2494 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BbvCI BfrBI BplI Bpu10I
BsiWI EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PfoI PmlI PsrI RsrII SacII SapI
SfiI SrfI SwaI