Restriction map for
pFN21AB5918
(Circular) (Six-base) MAPSORT of: pFN21AB5918.seq Check: 3488 from: 1 to: 6301
pFN21AB5918 6301 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4111 278
Size: 53 83 186 936 2575 2468
Fragments arranged by size:
2575 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3644 1937
Size: 1707 4594
Acc65I G'GTAC_C
Cuts at: 3622 3622
Size: 6301
AclI AA'CG_TT
Cuts at: 1563 4428 4801 1563
Size: 2865 373 3063
Fragments arranged by size:
3063 2865 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3098 4364 5748 6016 1632
Size: 1466 1266 1384 268 1917
Fragments arranged by size:
1917 1466 1384 1266 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6104
AflIII A'CryG_T
Cuts at: 1203 2870 5200 6177 1203
Size: 1667 2330 977 1327
Fragments arranged by size:
2330 1667 1327 977
AgeI A'CCGG_T
Cuts at: 5132 5132
Size: 6301
AhdI GACnn_n'nnGTC
Cuts at: 5030 5030
Size: 6301
AleI CACnn'nnGTG
Cuts at: 1166 2447 1166
Size: 1281 5020
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6301
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2714 2746 2714
Size: 32 6269
AlwNI CAG_nnn'CTG
Cuts at: 2075 2866 3052 3073 3341 4044 5616 2075
Size: 791 186 21 268 703 1572 2760
Fragments arranged by size:
2760 1572 791 703 268 186 21
ApaI G_GGCC'C
Cuts at: 3059 3067 3059
Size: 8 6293
ApaLI G'TGCA_C
Cuts at: 4358 5514 4358
Size: 1156 5145
ApoI r'AATT_y
Cuts at: 1493 3238 3610 3836 3988 6097 1493
Size: 1745 372 226 152 2109 1697
Fragments arranged by size:
2109 1745 1697 372 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4855 160
Size: 872 3823 1606
Fragments arranged by size:
3823 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6301
AvaI C'yCGr_G
Cuts at: 1870 1943 2054 2121 2430 2676 3058 3165 3284
Size: 73 111 67 309 246 382 107 119
Cuts at: 3284 3348 3626 1870
Size: 64 278 4545
Fragments arranged by size:
4545 382 309 278 246 119 111 107
73 67 64
BamHI G'GATC_C
Cuts at: 3631 3998 3631
Size: 367 5934
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2359 2425 2855 2938 3362
Size: 325 217 627 572 66 430 83 424
Cuts at: 3362 3622 5077 618
Size: 260 1455 1842
Fragments arranged by size:
1842 1455 627 572 430 424 325 260
217 83 66
BanII G_rGCy'C
Cuts at: 1391 2062 2502 3059 3067 3291 3620 1391
Size: 671 440 557 8 224 329 4072
Fragments arranged by size:
4072 671 557 440 329 224 8
BbeI G_GCGC'C
Cuts at: 2942 2942
Size: 6301
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5330
BbvCI CC'TCA_GC
Cuts at: 2505 3497 2505
Size: 992 5309
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2503 2537 4492 4526 1903
Size: 34 566 34 1955 34 3678
Fragments arranged by size:
3678 1955 566 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4193 5403 4193
Size: 1210 5091
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6301
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2590 4912 136
Size: 107 122 71 631 605 918 2322 1525
Fragments arranged by size:
2322 1525 918 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3352 6296 3352
Size: 2944 3357
BlpI GC'TnA_GC
Cuts at: 3718 3718
Size: 6301
Bme1580I G_kGCm'C
Cuts at: 1790 2428 2858 3059 3067 3365 3488 4362 5518
Size: 638 430 201 8 298 123 874 1156
Cuts at: 5518 1790
Size: 2573
Fragments arranged by size:
2573 1156 874 638 430 298 201 123
8
BmgBI CAC'GTC
Cuts at: 1318 2871 3228 1318
Size: 1553 357 4391
Fragments arranged by size:
4391 1553 357
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2729 2774 2894
Size: 416 53 83 399 24 1312 45 120
Cuts at: 2894 3062 4032 4990 442
Size: 168 970 958 1753
Fragments arranged by size:
1753 1312 970 958 416 399 168 120
83 53 45 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6301
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2629 2629
Size: 6301
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2325 2615 3149 4961 1239
Size: 1086 290 534 1812 2579
Fragments arranged by size:
2579 1812 1086 534 290
Bpu10I CC'TnA_GC
Cuts at: 2505 3497 2505
Size: 992 5309
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3275 3375 3377 4426 5291 5589 3275
Size: 100 2 1049 865 298 3987
Fragments arranged by size:
3987 1049 865 298 100 2
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2180 2974 4964 882
Size: 617 681 794 1990 2219
Fragments arranged by size:
2219 1990 794 681 617
BsaAI yAC'GTr
Cuts at: 493 1204 3504 4092 493
Size: 711 2300 588 2702
Fragments arranged by size:
2702 2300 711 588
BsaBI GATnn'nnATC
Cuts at: 3449 6295 3449
Size: 2846 3455
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2939 4108 4490 5125
Size: 53 83 186 936 1406 1169 382 635
Cuts at: 5125 275
Size: 1451
Fragments arranged by size:
1451 1406 1169 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2229 4734 5132 5406 5553 5964 2229
Size: 2505 398 274 147 411 2566
Fragments arranged by size:
2566 2505 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2118 2148 1178
Size: 30 165 30 715 30 5331
Fragments arranged by size:
5331 715 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2545 1186
Size: 1359 4942
BseYI C'CCAG_C
Cuts at: 2012 2859 3045 3066 3243 5504 6000 2012
Size: 847 186 21 177 2261 496 2313
Fragments arranged by size:
2313 2261 847 496 186 177 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2895 1163
Size: 573 1159 4569
Fragments arranged by size:
4569 1159 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3751 4512 5540 1540
Size: 275 186 1750 761 1028 2301
Fragments arranged by size:
2301 1750 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3291 3389 3429 4362 4447 5518 3291
Size: 98 40 933 85 1071 4074
Fragments arranged by size:
4074 1071 933 98 85 40
BsmI GAATG_Cn'
Cuts at: 3812 3905 3812
Size: 93 6208
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2062 2428 2502 2858 3059 3067 3291
Size: 399 272 366 74 356 201 8 224
Cuts at: 3291 3365 3389 3429 3488 3620 4362 4447 5518
Size: 74 24 40 59 132 742 85 1071
Cuts at: 5518 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 366 356 272 224
201 132 85 74 74 59 40 24
8
BspEI T'CCGG_A
Cuts at: 5964 5964
Size: 6301
BspHI T'CATG_A
Cuts at: 4190 5920 4190
Size: 1730 4571
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2586 3656 844
Size: 1742 1070 3489
Fragments arranged by size:
3489 1742 1070
BsrBI CCG'CTC
Cuts at: 1394 1823 4188 1394
Size: 429 2365 3507
Fragments arranged by size:
3507 2365 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2086 2257 3278 4796 4970 65
Size: 1183 838 171 1021 1518 174 1396
Fragments arranged by size:
1518 1396 1183 1021 838 174 171
BsrFI r'CCGG_y
Cuts at: 1700 1810 3137 4945 5132 1700
Size: 110 1327 1808 187 2869
Fragments arranged by size:
2869 1808 1327 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6301
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6301
BssSI C'ACGA_G
Cuts at: 2557 4361 5373 2557
Size: 1804 1012 3485
Fragments arranged by size:
3485 1804 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6301
BstBI TT'CG_AA
Cuts at: 5105 5105
Size: 6301
BstEII G'GTnAC_C
Cuts at: 1184 3400 3412 1184
Size: 2216 12 4073
Fragments arranged by size:
4073 2216 12
BstXI CCAn_nnnn'nTGG
Cuts at: 2176 2524 2176
Size: 348 5953
BstYI r'GATC_y
Cuts at: 1972 3352 3631 3998 4383 4400 5852 5938 5950
Size: 1380 279 367 385 17 1452 86 12
Cuts at: 5950 6296 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1380 385 367 346 279 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2445 2458 2517 2542 2706 513
Size: 552 939 441 13 59 25 164 4108
Fragments arranged by size:
4108 939 552 441 164 59 25 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5562
Fragments arranged by size:
5562 683 56
BtsI GCAGTG_nn'
Cuts at: 2906 3317 3486 3817 4611 4631 2906
Size: 411 169 331 794 20 4576
Fragments arranged by size:
4576 794 411 331 169 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6301
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6266
DraI TTT'AAA
Cuts at: 3605 3605
Size: 6301
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2095 5308 809
Size: 507 779 3213 1802
Fragments arranged by size:
3213 1802 779 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2817 3135 3139
Size: 54 1425 50 275 18 987 318 4
Cuts at: 3139 3230 3748 4637 6266 8
Size: 91 518 889 1629 43
Fragments arranged by size:
1629 1425 987 889 518 318 275 91
54 50 43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 3748 1537
Size: 275 1936 4090
Fragments arranged by size:
4090 1936 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4231 1353
Size: 2878 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4888 5262 5408 387
Size: 1064 138 3299 374 146 1280
Fragments arranged by size:
3299 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3289 3289
Size: 6301
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2325 2615 3098 3149 4364 4961 5748
Size: 393 693 290 483 51 1215 597 787
Cuts at: 5748 6016 1239
Size: 268 1524
Fragments arranged by size:
1524 1215 787 693 597 483 393 290
268 51
EcoNI CCTnn'n_nnAGG
Cuts at: 3077 3077
Size: 6301
EcoO109I rG'GnC_Cy
Cuts at: 2072 2680 3063 3564 2072
Size: 608 383 501 4809
Fragments arranged by size:
4809 608 501 383
EcoRI G'AATT_C
Cuts at: 3610 3988 3610
Size: 378 5923
FseI GG_CCGG'CC
Cuts at: 3141 3141
Size: 6301
FspI TGC'GCA
Cuts at: 4807 4807
Size: 6301
HaeII r_GCGC'y
Cuts at: 1678 2942 5448 1678
Size: 1264 2506 2531
Fragments arranged by size:
2531 2506 1264
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2288 2320 2521 2553 2798
Size: 32 135 32 716 32 201 32 245
Cuts at: 2798 2830 3196 3228 3331 3363 4942 4974 5016
Size: 32 366 32 103 32 1579 32 42
Cuts at: 5016 5048 1373
Size: 32 2626
Fragments arranged by size:
2626 1579 716 366 245 201 135 103
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2307 3645 3891 669
Size: 988 281 369 1338 246 3079
Fragments arranged by size:
3079 1338 988 369 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6301
HpaI GTT'AAC
Cuts at: 3891 3891
Size: 6301
KasI G'GCGC_C
Cuts at: 2938 2938
Size: 6301
KpnI G_GTAC'C
Cuts at: 3626 3626
Size: 6301
MfeI C'AATT_G
Cuts at: 3900 3900
Size: 6301
MluI A'CGCG_T
Cuts at: 6177 6177
Size: 6301
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5415 5599 1305
Size: 4110 184 2007
Fragments arranged by size:
4110 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3232 10
Size: 54 1425 343 1400 3079
Fragments arranged by size:
3079 1425 1400 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2447 3458 4259 4618 4777 518
Size: 648 1281 1011 801 359 159 2042
Fragments arranged by size:
2042 1281 1011 801 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2489 2504 2863 3523 3717 4394 5542 5787
Size: 883 15 359 660 194 677 1148 245
Cuts at: 5787 1606
Size: 2120
Fragments arranged by size:
2120 1148 883 677 660 359 245 194
15
NaeI GCC'GGC
Cuts at: 3139 3139
Size: 6301
NarI GG'CG_CC
Cuts at: 2939 2939
Size: 6301
NcoI C'CATG_G
Cuts at: 513 1065 2004 2542 513
Size: 552 939 538 4272
Fragments arranged by size:
4272 939 552 538
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6301
NgoMIV G'CCGG_C
Cuts at: 3137 3137
Size: 6301
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6301
NotI GC'GGCC_GC
Cuts at: 3748 3748
Size: 6301
NspI r_CATG'y
Cuts at: 1132 1229 2148 3659 5204 1132
Size: 97 919 1511 1545 2229
Fragments arranged by size:
2229 1545 1511 919 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6301
PciI A'CATG_T
Cuts at: 5200 5200
Size: 6301
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2075 2593 3052 1108
Size: 967 518 459 4357
Fragments arranged by size:
4357 967 518 459
PfoI T'CCnGG_A
Cuts at: 3214 3214
Size: 6301
PmeI GTTT'AAAC
Cuts at: 3605 3605
Size: 6301
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4370 4402 5883 5915 4370
Size: 32 1481 32 4756
Fragments arranged by size:
4756 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2072 2680 3564 2072
Size: 608 884 4809
Fragments arranged by size:
4809 884 608
PshAI GACnn'nnGTC
Cuts at: 1613 2639 2708 1613
Size: 1026 69 5206
Fragments arranged by size:
5206 1026 69
PsiI TTA'TAA
Cuts at: 3871 3871
Size: 6301
PspOMI G'GGCC_C
Cuts at: 3055 3063 3055
Size: 8 6293
PstI C_TGCA'G
Cuts at: 830 2913 3493 3653 830
Size: 2083 580 160 3478
Fragments arranged by size:
3478 2083 580 160
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6301
PvuII CAG'CTG
Cuts at: 2489 2489
Size: 6301
SacI G_AGCT'C
Cuts at: 3291 3291
Size: 6301
SalI G'TCGA_C
Cuts at: 1936 3643 1936
Size: 1707 4594
SanDI GG'GwC_CC
Cuts at: 3564 3564
Size: 6301
SbfI CC_TGCA'GG
Cuts at: 3653 3653
Size: 6301
ScaI AGT'ACT
Cuts at: 4549 6261 4549
Size: 1712 4589
SfcI C'TryA_G
Cuts at: 826 1045 2909 2926 3489 3649 4784 5465 5656
Size: 219 1864 17 563 160 1135 681 191
Cuts at: 5656 826
Size: 1471
Fragments arranged by size:
1864 1471 1135 681 563 219 191 160
17
SfoI GGC'GCC
Cuts at: 2940 2940
Size: 6301
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6301
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6301
SmaI CCC'GGG
Cuts at: 1872 2123 2432 2678 3167 3628 1872
Size: 251 309 246 489 461 4545
Fragments arranged by size:
4545 489 461 309 251 246
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3290 3348 3356 3390 4405 5306
Size: 197 926 1347 58 8 34 1015 901
Cuts at: 5306 5568 820
Size: 262 1553
Fragments arranged by size:
1553 1347 1015 926 901 262 197 58
34 8
SnaBI TAC'GTA
Cuts at: 493 4092 493
Size: 3599 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6301
SphI G_CATG'C
Cuts at: 1132 3659 1132
Size: 2527 3774
SspI AAT'ATT
Cuts at: 5 52 4225 5
Size: 47 4173 2081
Fragments arranged by size:
4173 2081 47
StuI AGG'CCT
Cuts at: 1517 2825 3495 1517
Size: 1308 670 4323
Fragments arranged by size:
4323 1308 670
StyI C'CwwG_G
Cuts at: 513 1065 2004 2542 2587 2820 3018 3740 513
Size: 552 939 538 45 233 198 722 3074
Fragments arranged by size:
3074 939 722 552 538 233 198 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2873 2996 4328 4345 4498 4683 5128
Size: 1255 1293 123 1332 17 153 185 445
Cuts at: 5128 325
Size: 1498
Fragments arranged by size:
1498 1332 1293 1255 445 185 153 123
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2331 4547 6259
Size: 274 80 33 51 1443 354 2216 1712
Cuts at: 6259 96
Size: 138
Fragments arranged by size:
2216 1712 1443 354 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2031 2063 2118 2150 6261 6293 2031
Size: 32 55 32 4111 32 2039
Fragments arranged by size:
4111 2039 55 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6301
XbaI T'CTAG_A
Cuts at: 3637 3637
Size: 6301
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2417 2443 2417
Size: 26 6275
XhoI C'TCGA_G
Cuts at: 1943 3348 1943
Size: 1405 4896
XmaI C'CCGG_G
Cuts at: 1870 2121 2430 2676 3165 3626 1870
Size: 251 309 246 489 461 4545
Fragments arranged by size:
4545 489 461 309 251 246
XmnI GAAnn'nnTTC
Cuts at: 4430 4430
Size: 6301
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4109 276
Size: 53 83 186 936 2575 2468
Fragments arranged by size:
2575 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoRV FalI FspAI NruI NsiI
PacI PmlI PspXI PsrI RsrII SacII SapI SexAI
SfiI SrfI SwaI