Restriction map for
pFN21AB5914
(Circular) (Six-base) MAPSORT of: pFN21AB5914.seq Check: 1708 from: 1 to: 6295
pFN21AB5914 6295 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4105 278
Size: 53 83 186 936 2569 2468
Fragments arranged by size:
2569 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2115 3638 1937
Size: 178 1523 4594
Fragments arranged by size:
4594 1523 178
Acc65I G'GTAC_C
Cuts at: 2679 3616 2679
Size: 937 5358
AclI AA'CG_TT
Cuts at: 1563 4422 4795 1563
Size: 2859 373 3063
Fragments arranged by size:
3063 2859 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4358 5742 6010 1632
Size: 2726 1384 268 1917
Fragments arranged by size:
2726 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6098
AflIII A'CryG_T
Cuts at: 1203 5194 6171 1203
Size: 3991 977 1327
Fragments arranged by size:
3991 1327 977
AgeI A'CCGG_T
Cuts at: 5126 5126
Size: 6295
AhdI GACnn_n'nnGTC
Cuts at: 5024 5024
Size: 6295
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6295
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6295
AlwNI CAG_nnn'CTG
Cuts at: 4038 5610 4038
Size: 1572 4723
ApaLI G'TGCA_C
Cuts at: 4352 5508 4352
Size: 1156 5139
ApoI r'AATT_y
Cuts at: 1493 3120 3604 3830 3982 6091 1493
Size: 1627 484 226 152 2109 1697
Fragments arranged by size:
2109 1697 1627 484 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4849 160
Size: 872 3817 1606
Fragments arranged by size:
3817 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6295
AvaI C'yCGr_G
Cuts at: 1870 1943 2763 2798 3620 1870
Size: 73 820 35 822 4545
Fragments arranged by size:
4545 822 820 73 35
BamHI G'GATC_C
Cuts at: 3625 3992 3625
Size: 367 5928
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2256 2679 3616 5071 618
Size: 325 217 627 469 423 937 1455 1842
Fragments arranged by size:
1842 1455 937 627 469 423 325 217
BanII G_rGCy'C
Cuts at: 1391 2798 2970 3575 3614 1391
Size: 1407 172 605 39 4072
Fragments arranged by size:
4072 1407 605 172 39
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5324
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4486 4520 1903
Size: 34 2549 34 3678
Fragments arranged by size:
3678 2549 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4187 5397 4187
Size: 1210 5085
BclI T'GATC_A
Cuts at: 1548 2847 2931 1548
Size: 1299 84 4912
Fragments arranged by size:
4912 1299 84
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2215 2735 4906
Size: 107 122 71 631 605 543 520 2171
Cuts at: 4906 136
Size: 1525
Fragments arranged by size:
2171 1525 631 605 543 520 122 107
71
BglII A'GATC_T
Cuts at: 3079 6290 3079
Size: 3211 3084
BlpI GC'TnA_GC
Cuts at: 2829 2969 3712 2829
Size: 140 743 5412
Fragments arranged by size:
5412 743 140
Bme1580I G_kGCm'C
Cuts at: 1790 2259 4356 5512 1790
Size: 469 2097 1156 2573
Fragments arranged by size:
2573 2097 1156 469
BmgBI CAC'GTC
Cuts at: 1318 3397 1318
Size: 2079 4216
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2853 3455 4026
Size: 416 53 83 399 24 1436 602 571
Cuts at: 4026 4984 442
Size: 958 1753
Fragments arranged by size:
1753 1436 958 602 571 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6295
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2104 2457 2104
Size: 353 5942
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2099 2843 3060 3500 4955 1239
Size: 860 744 217 440 1455 2579
Fragments arranged by size:
2579 1455 860 744 440 217
Bpu10I CC'TnA_GC
Cuts at: 2792 2792
Size: 6295
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4420 5285 5583 4420
Size: 865 298 5132
Fragments arranged by size:
5132 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4958 882
Size: 617 3459 2219
Fragments arranged by size:
3459 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3499 4086 493
Size: 711 2295 587 2702
Fragments arranged by size:
2702 2295 711 587
BsaBI GATnn'nnATC
Cuts at: 6289 6289
Size: 6295
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2787 4102 4484 5119
Size: 53 83 186 936 1254 1315 382 635
Cuts at: 5119 275
Size: 1451
Fragments arranged by size:
1451 1315 1254 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4728 5126 5400 5547 5958 4728
Size: 398 274 147 411 5065
Fragments arranged by size:
5065 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6070
Fragments arranged by size:
6070 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3334 1186
Size: 2148 4147
BseYI C'CCAG_C
Cuts at: 2180 2937 3348 5498 5994 2180
Size: 757 411 2150 496 2481
Fragments arranged by size:
2481 2150 757 496 411
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2705 3559 1163
Size: 573 969 854 3899
Fragments arranged by size:
3899 969 854 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3745 4506 5534 1540
Size: 275 186 1744 761 1028 2301
Fragments arranged by size:
2301 1744 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2085 2814 2970 4356 4441 5512 2085
Size: 729 156 1386 85 1071 2868
Fragments arranged by size:
2868 1386 1071 729 156 85
BsmI GAATG_Cn'
Cuts at: 2175 2532 3496 3806 3899 2175
Size: 357 964 310 93 4571
Fragments arranged by size:
4571 964 357 310 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2085 2259 2798 2814 2970 3575 3614
Size: 399 295 174 539 16 156 605 39
Cuts at: 3614 4356 4441 5512 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 605 539 399 295 174
156 85 39 16
BspEI T'CCGG_A
Cuts at: 5958 5958
Size: 6295
BspHI T'CATG_A
Cuts at: 4184 5914 4184
Size: 1730 4565
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3480 3650 844
Size: 2636 170 3489
Fragments arranged by size:
3489 2636 170
BsrBI CCG'CTC
Cuts at: 1394 1823 3336 4182 1394
Size: 429 1513 846 3507
Fragments arranged by size:
3507 1513 846 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2191 4790 4964 65
Size: 1183 943 2599 174 1396
Fragments arranged by size:
2599 1396 1183 943 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2592 2729 4939 5126 1700
Size: 110 782 137 2210 187 2869
Fragments arranged by size:
2869 2210 782 187 137 110
BsrGI T'GTAC_A
Cuts at: 96 2576 96
Size: 2480 3815
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6295
BssSI C'ACGA_G
Cuts at: 4355 5367 4355
Size: 1012 5283
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2604 2809 3361 794
Size: 1810 205 552 3728
Fragments arranged by size:
3728 1810 552 205
BstBI TT'CG_AA
Cuts at: 5099 5099
Size: 6295
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6295
BstXI CCAn_nnnn'nTGG
Cuts at: 3367 3367
Size: 6295
BstYI r'GATC_y
Cuts at: 1972 3079 3625 3992 4377 4394 5846 5932 5944
Size: 1107 546 367 385 17 1452 86 12
Cuts at: 5944 6290 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1107 546 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4804
Fragments arranged by size:
4804 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2264 487
Size: 683 56 1038 4518
Fragments arranged by size:
4518 1038 683 56
BtsI GCAGTG_nn'
Cuts at: 3811 4605 4625 3811
Size: 794 20 5481
Fragments arranged by size:
5481 794 20
ClaI AT'CG_AT
Cuts at: 1554 2583 1554
Size: 1029 5266
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2728 2763 529
Size: 35 2164 35 4061
Fragments arranged by size:
4061 2164 35 35
DraI TTT'AAA
Cuts at: 2524 3599 2524
Size: 1075 5220
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5302 809
Size: 507 3986 1802
Fragments arranged by size:
3986 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2243 2752 2907
Size: 54 1425 50 275 18 413 509 155
Cuts at: 2907 3742 4631 6260 8
Size: 835 889 1629 43
Fragments arranged by size:
1629 1425 889 835 509 413 275 155
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3742 1537
Size: 275 1930 4090
Fragments arranged by size:
4090 1930 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4225 1353
Size: 2872 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4882 5256 5402 387
Size: 1064 138 3293 374 146 1280
Fragments arranged by size:
3293 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2968 2968
Size: 6295
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2099 2843 3060 3500 4358 4955 5742
Size: 393 467 744 217 440 858 597 787
Cuts at: 5742 6010 1239
Size: 268 1524
Fragments arranged by size:
1524 858 787 744 597 467 440 393
268 217
EcoNI CCTnn'n_nnAGG
Cuts at: 2822 2822
Size: 6295
EcoRI G'AATT_C
Cuts at: 3120 3604 3982 3120
Size: 484 378 5433
Fragments arranged by size:
5433 484 378
EcoRV GAT'ATC
Cuts at: 2480 2480
Size: 6295
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3085 3085
Size: 6295
FspI TGC'GCA
Cuts at: 4801 4801
Size: 6295
HaeII r_GCGC'y
Cuts at: 1678 3043 5442 1678
Size: 1365 2399 2531
Fragments arranged by size:
2531 2399 1365
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2456 2488 4936 4968 5010
Size: 32 135 32 884 32 2448 32 42
Cuts at: 5010 5042 1373
Size: 32 2626
Fragments arranged by size:
2626 2448 884 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3639 3885 669
Size: 988 281 1701 246 3079
Fragments arranged by size:
3079 1701 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6295
HpaI GTT'AAC
Cuts at: 3885 3885
Size: 6295
KpnI G_GTAC'C
Cuts at: 2683 3620 2683
Size: 937 5358
MfeI C'AATT_G
Cuts at: 3045 3894 3045
Size: 849 5446
MluI A'CGCG_T
Cuts at: 6171 6171
Size: 6295
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2987 3205 5409 5593 1305
Size: 1682 218 2204 184 2007
Fragments arranged by size:
2204 2007 1682 218 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2245 10
Size: 54 1425 343 413 4060
Fragments arranged by size:
4060 1425 413 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2298 2888 4253 4612 4771 518
Size: 648 1132 590 1365 359 159 2042
Fragments arranged by size:
2042 1365 1132 648 590 359 159
MspA1I CmG'CkG
Cuts at: 1606 2719 2937 3711 4388 5536 5781 1606
Size: 1113 218 774 677 1148 245 2120
Fragments arranged by size:
2120 1148 1113 774 677 245 218
NaeI GCC'GGC
Cuts at: 2731 2731
Size: 6295
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4804
Fragments arranged by size:
4804 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6295
NgoMIV G'CCGG_C
Cuts at: 2729 2729
Size: 6295
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6295
NotI GC'GGCC_GC
Cuts at: 3742 3742
Size: 6295
NspI r_CATG'y
Cuts at: 1132 1229 3142 3407 3653 5198 1132
Size: 97 1913 265 246 1545 2229
Fragments arranged by size:
2229 1913 1545 265 246 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6295
PciI A'CATG_T
Cuts at: 5194 5194
Size: 6295
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2252 1108
Size: 1144 5151
PfoI T'CCnGG_A
Cuts at: 2660 2820 2660
Size: 160 6135
PmeI GTTT'AAAC
Cuts at: 3599 3599
Size: 6295
PmlI CAC'GTG
Cuts at: 3499 3499
Size: 6295
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3417 3449 4364 4396 5877 5909 3417
Size: 32 915 32 1481 32 3803
Fragments arranged by size:
3803 1481 915 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6295
PsiI TTA'TAA
Cuts at: 3865 3865
Size: 6295
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2337 2369 2337
Size: 32 6263
PstI C_TGCA'G
Cuts at: 830 2060 2462 3647 830
Size: 1230 402 1185 3478
Fragments arranged by size:
3478 1230 1185 402
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6295
PvuII CAG'CTG
Cuts at: 2719 2937 2719
Size: 218 6077
SacI G_AGCT'C
Cuts at: 2970 2970
Size: 6295
SalI G'TCGA_C
Cuts at: 1936 3637 1936
Size: 1701 4594
SbfI CC_TGCA'GG
Cuts at: 3647 3647
Size: 6295
ScaI AGT'ACT
Cuts at: 4543 6255 4543
Size: 1712 4583
SfcI C'TryA_G
Cuts at: 826 1045 2056 2149 2458 3643 4778 5459 5650
Size: 219 1011 93 309 1185 1135 681 191
Cuts at: 5650 826
Size: 1471
Fragments arranged by size:
1471 1185 1135 1011 681 309 219 191
93
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6295
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6295
SmaI CCC'GGG
Cuts at: 1872 3622 1872
Size: 1750 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4399 5300 5562 820
Size: 197 926 2456 901 262 1553
Fragments arranged by size:
2456 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4086 493
Size: 3593 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6295
SphI G_CATG'C
Cuts at: 1132 3407 3653 1132
Size: 2275 246 3774
Fragments arranged by size:
3774 2275 246
SspI AAT'ATT
Cuts at: 5 52 4219 5
Size: 47 4167 2081
Fragments arranged by size:
4167 2081 47
StuI AGG'CCT
Cuts at: 1517 2516 2612 2879 1517
Size: 999 96 267 4933
Fragments arranged by size:
4933 999 267 96
StyI C'CwwG_G
Cuts at: 513 1065 2004 3734 513
Size: 552 939 1730 3074
Fragments arranged by size:
3074 1730 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3310 3320 4322 4339 4492 4677 5122
Size: 1255 1730 10 1002 17 153 185 445
Cuts at: 5122 325
Size: 1498
Fragments arranged by size:
1730 1498 1255 1002 445 185 153 17
10
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2576 4541 6253
Size: 274 80 33 51 1443 599 1965 1712
Cuts at: 6253 96
Size: 138
Fragments arranged by size:
1965 1712 1443 599 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2374 2406 6255 6287 2374
Size: 32 3849 32 2382
Fragments arranged by size:
3849 2382 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6295
XbaI T'CTAG_A
Cuts at: 3631 3631
Size: 6295
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2074 2238 2074
Size: 164 6131
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6295
XmaI C'CCGG_G
Cuts at: 1870 3620 1870
Size: 1750 4545
XmnI GAAnn'nnTTC
Cuts at: 3120 4424 3120
Size: 1304 4991
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4103 276
Size: 53 83 186 936 2569 2468
Fragments arranged by size:
2569 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PsrI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoO109I
FseI FspAI KasI NarI NruI NsiI PacI PpuMI
PspOMI PspXI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI