Restriction map for
pFN21AB5912
(Circular) (Six-base) MAPSORT of: pFN21AB5912.seq Check: 9770 from: 1 to: 6292
pFN21AB5912 6292 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2564 2564
Size: 6292
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4102 278
Size: 53 83 186 936 2566 2468
Fragments arranged by size:
2566 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3635 1937
Size: 1698 4594
Acc65I G'GTAC_C
Cuts at: 3613 3613
Size: 6292
AclI AA'CG_TT
Cuts at: 1563 3519 4419 4792 1563
Size: 1956 900 373 3063
Fragments arranged by size:
3063 1956 900 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2168 3561 4355 5739 6007 1632
Size: 536 1393 794 1384 268 1917
Fragments arranged by size:
1917 1393 1384 794 536 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6095
AflIII A'CryG_T
Cuts at: 1203 5191 6168 1203
Size: 3988 977 1327
Fragments arranged by size:
3988 1327 977
AgeI A'CCGG_T
Cuts at: 5123 5123
Size: 6292
AhdI GACnn_n'nnGTC
Cuts at: 5021 5021
Size: 6292
AleI CACnn'nnGTG
Cuts at: 1166 2538 1166
Size: 1372 4920
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2833 1184
Size: 1649 4643
AlwNI CAG_nnn'CTG
Cuts at: 2146 2651 4035 5607 2146
Size: 505 1384 1572 2831
Fragments arranged by size:
2831 1572 1384 505
ApaLI G'TGCA_C
Cuts at: 4349 5505 4349
Size: 1156 5136
ApoI r'AATT_y
Cuts at: 1493 2372 3601 3827 3979 6088 1493
Size: 879 1229 226 152 2109 1697
Fragments arranged by size:
2109 1697 1229 879 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4846 160
Size: 872 3814 1606
Fragments arranged by size:
3814 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6292
AvaI C'yCGr_G
Cuts at: 1870 1943 3617 1870
Size: 73 1674 4545
Fragments arranged by size:
4545 1674 73
BamHI G'GATC_C
Cuts at: 3622 3989 3622
Size: 367 5925
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2489 3613 5068 618
Size: 325 217 627 702 1124 1455 1842
Fragments arranged by size:
1842 1455 1124 702 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2560 3574 3611 1391
Size: 1169 1014 37 4072
Fragments arranged by size:
4072 1169 1014 37
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2036 928
Size: 971 137 5184
Fragments arranged by size:
5184 971 137
BbvCI CC'TCA_GC
Cuts at: 2117 2117
Size: 6292
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4483 4517 1903
Size: 34 2546 34 3678
Fragments arranged by size:
3678 2546 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4184 5394 4184
Size: 1210 5082
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6292
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4903 136
Size: 107 122 71 631 605 3231 1525
Fragments arranged by size:
3231 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6287 6287
Size: 6292
BlpI GC'TnA_GC
Cuts at: 3709 3709
Size: 6292
Bme1580I G_kGCm'C
Cuts at: 1790 2222 2434 2892 4353 5509 1790
Size: 432 212 458 1461 1156 2573
Fragments arranged by size:
2573 1461 1156 458 432 212
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6292
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4023 4981 442
Size: 416 53 83 399 24 2606 958 1753
Fragments arranged by size:
2606 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6292
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4952 1239
Size: 3713 2579
Bpu10I CC'TnA_GC
Cuts at: 2117 2117
Size: 6292
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2280 4417 5282 5580 2280
Size: 2137 865 298 2992
Fragments arranged by size:
2992 2137 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4955 882
Size: 617 3456 2219
Fragments arranged by size:
3456 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2684 3072 4083 493
Size: 711 1480 388 1011 2702
Fragments arranged by size:
2702 1480 1011 711 388
BsaBI GATnn'nnATC
Cuts at: 6286 6286
Size: 6292
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2089 4099 4481 5116
Size: 53 83 186 936 556 2010 382 635
Cuts at: 5116 275
Size: 1451
Fragments arranged by size:
2010 1451 936 635 556 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4725 5123 5397 5544 5955 4725
Size: 398 274 147 411 5062
Fragments arranged by size:
5062 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6067
Fragments arranged by size:
6067 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2167 1186
Size: 981 5311
BseYI C'CCAG_C
Cuts at: 2497 2820 3026 5495 5991 2497
Size: 323 206 2469 496 2798
Fragments arranged by size:
2798 2469 496 323 206
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2637 2715 1163
Size: 573 901 78 4740
Fragments arranged by size:
4740 901 573 78
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3100 3742 4503 5531 1540
Size: 275 186 1099 642 761 1028 2301
Fragments arranged by size:
2301 1099 1028 761 642 275 186
BsiHKAI G_wGCw'C
Cuts at: 3013 4353 4438 5509 3013
Size: 1340 85 1071 3796
Fragments arranged by size:
3796 1340 1071 85
BsmI GAATG_Cn'
Cuts at: 2026 2501 3803 3896 2026
Size: 475 1302 93 4422
Fragments arranged by size:
4422 1302 475 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2222 2434 2560 2892 3013 3574 3611
Size: 399 432 212 126 332 121 561 37
Cuts at: 3611 4353 4438 5509 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 561 432 399 332 212
126 121 85 37
BspEI T'CCGG_A
Cuts at: 5955 5955
Size: 6292
BspHI T'CATG_A
Cuts at: 2788 4181 5911 2788
Size: 1393 1730 3169
Fragments arranged by size:
3169 1730 1393
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2564 2825 3647 844
Size: 1720 261 822 3489
Fragments arranged by size:
3489 1720 822 261
BsrBI CCG'CTC
Cuts at: 1394 1823 4179 1394
Size: 429 2356 3507
Fragments arranged by size:
3507 2356 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2275 2442 2568 4787 4961 65
Size: 1183 1027 167 126 2219 174 1396
Fragments arranged by size:
2219 1396 1183 1027 174 167 126
BsrFI r'CCGG_y
Cuts at: 1700 1810 3095 4936 5123 1700
Size: 110 1285 1841 187 2869
Fragments arranged by size:
2869 1841 1285 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6292
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6292
BssSI C'ACGA_G
Cuts at: 4352 5364 4352
Size: 1012 5280
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6292
BstBI TT'CG_AA
Cuts at: 5096 5096
Size: 6292
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6292
BstXI CCAn_nnnn'nTGG
Cuts at: 2476 2476
Size: 6292
BstYI r'GATC_y
Cuts at: 1972 3622 3989 4374 4391 5843 5929 5941 6287
Size: 1650 367 385 17 1452 86 12 346
Cuts at: 6287 1972
Size: 1977
Fragments arranged by size:
1977 1650 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5740
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5553
Fragments arranged by size:
5553 683 56
BtsI GCAGTG_nn'
Cuts at: 3808 4602 4622 3808
Size: 794 20 5478
Fragments arranged by size:
5478 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6292
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6257
DraI TTT'AAA
Cuts at: 3596 3596
Size: 6292
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5299 809
Size: 507 3983 1802
Fragments arranged by size:
3983 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3097 3739 4628
Size: 54 1425 50 275 18 1267 642 889
Cuts at: 4628 6257 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1267 889 642 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3097 3739 1537
Size: 275 1285 642 4090
Fragments arranged by size:
4090 1285 642 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2144 3498 4222 1353
Size: 791 1354 724 3423
Fragments arranged by size:
3423 1354 791 724
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4879 5253 5399 387
Size: 1064 138 3290 374 146 1280
Fragments arranged by size:
3290 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2168 3561 4355 4952 5739 6007 1239
Size: 393 536 1393 794 597 787 268 1524
Fragments arranged by size:
1524 1393 794 787 597 536 393 268
EcoO109I rG'GnC_Cy
Cuts at: 3579 3579
Size: 6292
EcoRI G'AATT_C
Cuts at: 3601 3979 3601
Size: 378 5914
FspI TGC'GCA
Cuts at: 4798 4798
Size: 6292
HaeII r_GCGC'y
Cuts at: 1678 5439 1678
Size: 3761 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2478 2510 2645 2677 4933
Size: 32 135 32 906 32 135 32 2256
Cuts at: 4933 4965 5007 5039 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2256 906 135 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2623 3636 3882 669
Size: 988 281 685 1013 246 3079
Fragments arranged by size:
3079 1013 988 685 281 246
HindIII A'AGCT_T
Cuts at: 748 2903 3472 748
Size: 2155 569 3568
Fragments arranged by size:
3568 2155 569
HpaI GTT'AAC
Cuts at: 3882 3882
Size: 6292
KpnI G_GTAC'C
Cuts at: 3617 3617
Size: 6292
MfeI C'AATT_G
Cuts at: 2928 3891 2928
Size: 963 5329
MluI A'CGCG_T
Cuts at: 6168 6168
Size: 6292
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5406 5590 1305
Size: 4101 184 2007
Fragments arranged by size:
4101 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4470
Fragments arranged by size:
4470 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2538 2790 2870 3444 4250 4609 4768
Size: 648 1372 252 80 574 806 359 159
Cuts at: 4768 518
Size: 2042
Fragments arranged by size:
2042 1372 806 648 574 359 252 159
80
MspA1I CmG'CkG
Cuts at: 1606 2036 2194 3708 4385 5533 5778 1606
Size: 430 158 1514 677 1148 245 2120
Fragments arranged by size:
2120 1514 1148 677 430 245 158
NaeI GCC'GGC
Cuts at: 3097 3097
Size: 6292
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5740
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6292
NgoMIV G'CCGG_C
Cuts at: 3095 3095
Size: 6292
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6292
NotI GC'GGCC_GC
Cuts at: 3739 3739
Size: 6292
NspI r_CATG'y
Cuts at: 1132 1229 2673 3650 5195 1132
Size: 97 1444 977 1545 2229
Fragments arranged by size:
2229 1545 1444 977 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6292
PciI A'CATG_T
Cuts at: 5191 5191
Size: 6292
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6292
PfoI T'CCnGG_A
Cuts at: 3324 3324
Size: 6292
PmeI GTTT'AAAC
Cuts at: 3596 3596
Size: 6292
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4361 4393 5874 5906 4361
Size: 32 1481 32 4747
Fragments arranged by size:
4747 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3579 3579
Size: 6292
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6292
PsiI TTA'TAA
Cuts at: 3862 3862
Size: 6292
PstI C_TGCA'G
Cuts at: 830 2199 3644 830
Size: 1369 1445 3478
Fragments arranged by size:
3478 1445 1369
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6292
PvuII CAG'CTG
Cuts at: 2036 2036
Size: 6292
SalI G'TCGA_C
Cuts at: 1936 3634 1936
Size: 1698 4594
SbfI CC_TGCA'GG
Cuts at: 3644 3644
Size: 6292
ScaI AGT'ACT
Cuts at: 4540 6252 4540
Size: 1712 4580
SfcI C'TryA_G
Cuts at: 826 1045 2195 3495 3640 4775 5456 5647 826
Size: 219 1150 1300 145 1135 681 191 1471
Fragments arranged by size:
1471 1300 1150 1135 681 219 191 145
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6292
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6292
SmaI CCC'GGG
Cuts at: 1872 3619 1872
Size: 1747 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2295 4396 5297 5559 820
Size: 197 926 352 2101 901 262 1553
Fragments arranged by size:
2101 1553 926 901 352 262 197
SnaBI TAC'GTA
Cuts at: 493 2684 4083 493
Size: 2191 1399 2702
Fragments arranged by size:
2702 2191 1399
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6292
SphI G_CATG'C
Cuts at: 1132 3650 1132
Size: 2518 3774
SspI AAT'ATT
Cuts at: 5 52 2962 4216 5
Size: 47 2910 1254 2081
Fragments arranged by size:
2910 2081 1254 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6292
StyI C'CwwG_G
Cuts at: 513 1065 2469 3731 513
Size: 552 1404 1262 3074
Fragments arranged by size:
3074 1404 1262 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4319 4336 4489 4674 5119 325
Size: 1255 2739 17 153 185 445 1498
Fragments arranged by size:
2739 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2075 4538 6250
Size: 274 80 33 51 1443 98 2463 1712
Cuts at: 6250 96
Size: 138
Fragments arranged by size:
2463 1712 1443 274 138 98 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3056 3088 3182 3214 6252 6284 3056
Size: 32 94 32 3038 32 3064
Fragments arranged by size:
3064 3038 94 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2246 3079 1316
Size: 930 833 4529
Fragments arranged by size:
4529 930 833
XbaI T'CTAG_A
Cuts at: 3628 3628
Size: 6292
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3313 3563 3313
Size: 250 6042
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6292
XmaI C'CCGG_G
Cuts at: 1870 3617 1870
Size: 1747 4545
XmnI GAAnn'nnTTC
Cuts at: 3488 4421 3488
Size: 933 5359
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4100 276
Size: 53 83 186 936 2566 2468
Fragments arranged by size:
2566 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI