Restriction map for
pFN21AB5908
(Circular) (Six-base) MAPSORT of: pFN21AB5908.seq Check: 625 from: 1 to: 6265
pFN21AB5908 6265 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2326 2326
Size: 6265
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4075 278
Size: 53 83 186 936 2539 2468
Fragments arranged by size:
2539 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3608 1937
Size: 1671 4594
Acc65I G'GTAC_C
Cuts at: 3586 3586
Size: 6265
AclI AA'CG_TT
Cuts at: 1563 4392 4765 1563
Size: 2829 373 3063
Fragments arranged by size:
3063 2829 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2120 2910 3278 4328 5712 5980 1632
Size: 488 790 368 1050 1384 268 1917
Fragments arranged by size:
1917 1384 1050 790 488 368 268
AflII C'TTAA_G
Cuts at: 820 1017 2354 820
Size: 197 1337 4731
Fragments arranged by size:
4731 1337 197
AflIII A'CryG_T
Cuts at: 1203 5164 6141 1203
Size: 3961 977 1327
Fragments arranged by size:
3961 1327 977
AgeI A'CCGG_T
Cuts at: 2515 5096 2515
Size: 2581 3684
AhdI GACnn_n'nnGTC
Cuts at: 4994 4994
Size: 6265
AleI CACnn'nnGTG
Cuts at: 1166 2336 3561 1166
Size: 1170 1225 3870
Fragments arranged by size:
3870 1225 1170
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6265
AlwNI CAG_nnn'CTG
Cuts at: 2242 2888 4008 5580 2242
Size: 646 1120 1572 2927
Fragments arranged by size:
2927 1572 1120 646
ApaLI G'TGCA_C
Cuts at: 4322 5478 4322
Size: 1156 5109
ApoI r'AATT_y
Cuts at: 1493 3574 3800 3952 6061 1493
Size: 2081 226 152 2109 1697
Fragments arranged by size:
2109 2081 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4819 160
Size: 872 3787 1606
Fragments arranged by size:
3787 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6265
AvaI C'yCGr_G
Cuts at: 1870 1943 2776 3183 3590 1870
Size: 73 833 407 407 4545
Fragments arranged by size:
4545 833 407 407 73
BamHI G'GATC_C
Cuts at: 2740 3419 3595 3962 2740
Size: 679 176 367 5043
Fragments arranged by size:
5043 679 367 176
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2338 2518 3344 3586 5041
Size: 325 217 627 551 180 826 242 1455
Cuts at: 5041 618
Size: 1842
Fragments arranged by size:
1842 1455 826 627 551 325 242 217
180
BanII G_rGCy'C
Cuts at: 1391 2067 2880 3190 3584 1391
Size: 676 813 310 394 4072
Fragments arranged by size:
4072 813 676 394 310
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3438 928
Size: 971 1539 3755
Fragments arranged by size:
3755 1539 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4456 4490 1903
Size: 34 50 34 2435 34 3678
Fragments arranged by size:
3678 2435 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4157 5367 4157
Size: 1210 5055
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6265
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4876 136
Size: 107 122 71 631 605 3204 1525
Fragments arranged by size:
3204 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6260 6260
Size: 6265
BlpI GC'TnA_GC
Cuts at: 3682 3682
Size: 6265
Bme1580I G_kGCm'C
Cuts at: 1790 2277 2313 2633 4326 5482 1790
Size: 487 36 320 1693 1156 2573
Fragments arranged by size:
2573 1693 1156 487 320 36
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6265
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2248 3384 3996
Size: 416 53 83 399 24 831 1136 612
Cuts at: 3996 4954 442
Size: 958 1753
Fragments arranged by size:
1753 1136 958 831 612 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6265
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2140 3149 3213 4925 1239
Size: 901 1009 64 1712 2579
Fragments arranged by size:
2579 1712 1009 901 64
Bpu10I CC'TnA_GC
Cuts at: 2471 2471
Size: 6265
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3290 4390 5255 5553 3290
Size: 1100 865 298 4002
Fragments arranged by size:
4002 1100 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3224 4928 882
Size: 617 1725 1704 2219
Fragments arranged by size:
2219 1725 1704 617
BsaAI yAC'GTr
Cuts at: 493 1204 4056 493
Size: 711 2852 2702
Fragments arranged by size:
2852 2702 711
BsaBI GATnn'nnATC
Cuts at: 6259 6259
Size: 6265
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4072 4454 5089 275
Size: 53 83 186 936 2539 382 635 1451
Fragments arranged by size:
2539 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2515 4698 5096 5370 5517 5928 2515
Size: 2183 398 274 147 411 2852
Fragments arranged by size:
2852 2183 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3442 3472 3517 3547 1178
Size: 30 165 30 2039 30 45 30 3896
Fragments arranged by size:
3896 2039 165 45 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2063 2143 3541 1186
Size: 877 80 1398 3910
Fragments arranged by size:
3910 1398 877 80
BseYI C'CCAG_C
Cuts at: 5468 5964 5468
Size: 496 5769
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2381 1163
Size: 573 645 5047
Fragments arranged by size:
5047 645 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3715 4476 5504 1540
Size: 275 186 1714 761 1028 2301
Fragments arranged by size:
2301 1714 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2880 2902 3135 3190 3558 4326 4411 5482 2880
Size: 22 233 55 368 768 85 1071 3663
Fragments arranged by size:
3663 1071 768 368 233 85 55 22
BsmI GAATG_Cn'
Cuts at: 3776 3869 3776
Size: 93 6172
BsmBI CGTCTCn'nnnn_
Cuts at: 3542 3542
Size: 6265
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2067 2277 2313 2633 2880 2902 3135
Size: 399 277 210 36 320 247 22 233
Cuts at: 3135 3190 3558 3584 4326 4411 5482 1391
Size: 55 368 26 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 368 320 277 247
233 210 85 55 36 26 22
BspEI T'CCGG_A
Cuts at: 5928 5928
Size: 6265
BspHI T'CATG_A
Cuts at: 2056 4154 5884 2056
Size: 2098 1730 2437
Fragments arranged by size:
2437 2098 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2326 2407 3620 844
Size: 1482 81 1213 3489
Fragments arranged by size:
3489 1482 1213 81
BsrBI CCG'CTC
Cuts at: 1394 1823 4152 1394
Size: 429 2329 3507
Fragments arranged by size:
3507 2329 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4760 4934 65
Size: 1183 3512 174 1396
Fragments arranged by size:
3512 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2515 3141 4909 5096 1700
Size: 110 705 626 1768 187 2869
Fragments arranged by size:
2869 1768 705 626 187 110
BsrGI T'GTAC_A
Cuts at: 96 3317 96
Size: 3221 3044
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6265
BssSI C'ACGA_G
Cuts at: 2652 4325 5337 2652
Size: 1673 1012 3580
Fragments arranged by size:
3580 1673 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2194 2242 3151 794
Size: 1400 48 909 3908
Fragments arranged by size:
3908 1400 909 48
BstBI TT'CG_AA
Cuts at: 2037 5069 2037
Size: 3032 3233
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6265
BstXI CCAn_nnnn'nTGG
Cuts at: 3415 3415
Size: 6265
BstYI r'GATC_y
Cuts at: 1972 2740 3419 3595 3962 4347 4364 5816 5902
Size: 768 679 176 367 385 17 1452 86
Cuts at: 5902 5914 6260 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 768 679 385 367 346 176
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2547 3057 3213 513
Size: 552 1482 510 156 3565
Fragments arranged by size:
3565 1482 552 510 156
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5526
Fragments arranged by size:
5526 683 56
BtsI GCAGTG_nn'
Cuts at: 3781 4575 4595 3781
Size: 794 20 5451
Fragments arranged by size:
5451 794 20
ClaI AT'CG_AT
Cuts at: 1554 2685 1554
Size: 1131 5134
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6230
DraI TTT'AAA
Cuts at: 3569 3569
Size: 6265
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5272 809
Size: 507 3956 1802
Fragments arranged by size:
3956 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2904 3712 4601
Size: 54 1425 50 275 18 1074 808 889
Cuts at: 4601 6230 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1074 889 808 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3712 1537
Size: 275 1900 4090
Fragments arranged by size:
4090 1900 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2055 2096 2598 3540 4195 1353
Size: 702 41 502 942 655 3423
Fragments arranged by size:
3423 942 702 655 502 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2104 4852 5226 5372 387
Size: 1064 138 515 2748 374 146 1280
Fragments arranged by size:
2748 1280 1064 515 374 146 138
EcoICRI GAG'CTC
Cuts at: 2878 3188 2878
Size: 310 5955
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2120 2140 2910 3149 3213 3278 4328
Size: 393 488 20 770 239 64 65 1050
Cuts at: 4328 4925 5712 5980 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1050 787 770 597 488 393 268
239 65 64 20
EcoO109I rG'GnC_Cy
Cuts at: 2130 2130
Size: 6265
EcoRI G'AATT_C
Cuts at: 3574 3952 3574
Size: 378 5887
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3308 3308
Size: 6265
FspI TGC'GCA
Cuts at: 4771 4771
Size: 6265
HaeII r_GCGC'y
Cuts at: 1678 5412 1678
Size: 3734 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4906 4938 4980 5012 1373
Size: 32 135 32 3334 32 42 32 2626
Fragments arranged by size:
3334 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2093 3609 3855 669
Size: 988 281 155 1516 246 3079
Fragments arranged by size:
3079 1516 988 281 246 155
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6265
HpaI GTT'AAC
Cuts at: 3855 3855
Size: 6265
KpnI G_GTAC'C
Cuts at: 3590 3590
Size: 6265
MfeI C'AATT_G
Cuts at: 3864 3864
Size: 6265
MluI A'CGCG_T
Cuts at: 6141 6141
Size: 6265
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2595 5379 5563 1305
Size: 1290 2784 184 2007
Fragments arranged by size:
2784 2007 1290 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2906 10
Size: 54 1425 343 1074 3369
Fragments arranged by size:
3369 1425 1074 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2336 2552 3413 3440 3561 4223 4582
Size: 648 1170 216 861 27 121 662 359
Cuts at: 4582 4741 518
Size: 159 2042
Fragments arranged by size:
2042 1170 861 662 648 359 216 159
121 27
MspA1I CmG'CkG
Cuts at: 1606 2098 3681 4358 5506 5751 1606
Size: 492 1583 677 1148 245 2120
Fragments arranged by size:
2120 1583 1148 677 492 245
NcoI C'CATG_G
Cuts at: 513 1065 2547 513
Size: 552 1482 4231
Fragments arranged by size:
4231 1482 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6265
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6265
NotI GC'GGCC_GC
Cuts at: 3712 3712
Size: 6265
NspI r_CATG'y
Cuts at: 1132 1229 2459 3025 3623 5168 1132
Size: 97 1230 566 598 1545 2229
Fragments arranged by size:
2229 1545 1230 598 566 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6265
PciI A'CATG_T
Cuts at: 5164 5164
Size: 6265
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6265
PmeI GTTT'AAAC
Cuts at: 3569 3569
Size: 6265
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2095 2127 4334 4366 5847 5879 2095
Size: 32 2207 32 1481 32 2481
Fragments arranged by size:
2481 2207 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6265
PsiI TTA'TAA
Cuts at: 2595 2715 3835 2595
Size: 120 1120 5025
Fragments arranged by size:
5025 1120 120
PspXI vC'TCGA_Gb
Cuts at: 3183 3183
Size: 6265
PstI C_TGCA'G
Cuts at: 830 2247 3281 3617 830
Size: 1417 1034 336 3478
Fragments arranged by size:
3478 1417 1034 336
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6265
SacI G_AGCT'C
Cuts at: 2880 3190 2880
Size: 310 5955
SalI G'TCGA_C
Cuts at: 1936 3607 1936
Size: 1671 4594
SapI GCTCTTCn'nnn_
Cuts at: 2055 2096 2598 2055
Size: 41 502 5722
Fragments arranged by size:
5722 502 41
SbfI CC_TGCA'GG
Cuts at: 3617 3617
Size: 6265
ScaI AGT'ACT
Cuts at: 4513 6225 4513
Size: 1712 4553
SfcI C'TryA_G
Cuts at: 826 1045 2243 3277 3397 3613 4748 5429 5620
Size: 219 1198 1034 120 216 1135 681 191
Cuts at: 5620 826
Size: 1471
Fragments arranged by size:
1471 1198 1135 1034 681 219 216 191
120
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6265
SgrAI Cr'CCGG_yG
Cuts at: 1700 2515 1700
Size: 815 5450
SmaI CCC'GGG
Cuts at: 1872 3592 1872
Size: 1720 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2354 3183 3269 4369 5270 5532
Size: 197 926 411 829 86 1100 901 262
Cuts at: 5532 820
Size: 1553
Fragments arranged by size:
1553 1100 926 901 829 411 262 197
86
SnaBI TAC'GTA
Cuts at: 493 4056 493
Size: 3563 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6265
SphI G_CATG'C
Cuts at: 1132 3623 1132
Size: 2491 3774
SspI AAT'ATT
Cuts at: 5 52 4189 5
Size: 47 4137 2081
Fragments arranged by size:
4137 2081 47
StuI AGG'CCT
Cuts at: 1517 2826 1517
Size: 1309 4956
StyI C'CwwG_G
Cuts at: 513 1065 2547 2806 3704 513
Size: 552 1482 259 898 3074
Fragments arranged by size:
3074 1482 898 552 259
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2551 4292 4309 4462 4647 5092 325
Size: 1255 971 1741 17 153 185 445 1498
Fragments arranged by size:
1741 1498 1255 971 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3317 4511 6223
Size: 274 80 33 51 1443 1340 1194 1712
Cuts at: 6223 96
Size: 138
Fragments arranged by size:
1712 1443 1340 1194 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2305 2337 3442 3474 6225 6257 2305
Size: 32 1105 32 2751 32 2313
Fragments arranged by size:
2751 2313 1105 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6265
XbaI T'CTAG_A
Cuts at: 3601 3601
Size: 6265
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2545 3024 2545
Size: 479 5786
XhoI C'TCGA_G
Cuts at: 1943 3183 1943
Size: 1240 5025
XmaI C'CCGG_G
Cuts at: 1870 3590 1870
Size: 1720 4545
XmnI GAAnn'nnTTC
Cuts at: 3264 4394 3264
Size: 1130 5135
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4073 276
Size: 53 83 186 936 2539 2468
Fragments arranged by size:
2539 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspXI PstI PvuI SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PsrI PvuII RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI