Restriction map for
pFN21AB5905
(Circular) (Six-base) MAPSORT of: pFN21AB5905.seq Check: 4368 from: 1 to: 6211
pFN21AB5905 6211 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4021 278
Size: 53 83 186 936 2485 2468
Fragments arranged by size:
2485 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3554 1937
Size: 1617 4594
Acc65I G'GTAC_C
Cuts at: 3532 3532
Size: 6211
AclI AA'CG_TT
Cuts at: 1563 4338 4711 1563
Size: 2775 373 3063
Fragments arranged by size:
3063 2775 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2897 2930 4274 5658 5926 1632
Size: 1265 33 1344 1384 268 1917
Fragments arranged by size:
1917 1384 1344 1265 268 33
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6014
AflIII A'CryG_T
Cuts at: 1203 3201 5110 6087 1203
Size: 1998 1909 977 1327
Fragments arranged by size:
1998 1909 1327 977
AgeI A'CCGG_T
Cuts at: 3369 5042 3369
Size: 1673 4538
AhdI GACnn_n'nnGTC
Cuts at: 4940 4940
Size: 6211
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6211
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6211
AlwNI CAG_nnn'CTG
Cuts at: 3954 5526 3954
Size: 1572 4639
ApaLI G'TGCA_C
Cuts at: 4268 5424 4268
Size: 1156 5055
ApoI r'AATT_y
Cuts at: 1493 3071 3520 3746 3898 6007 1493
Size: 1578 449 226 152 2109 1697
Fragments arranged by size:
2109 1697 1578 449 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4765 160
Size: 872 3733 1606
Fragments arranged by size:
3733 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6211
AvaI C'yCGr_G
Cuts at: 1870 1943 3114 3536 1870
Size: 73 1171 422 4545
Fragments arranged by size:
4545 1171 422 73
AvrII C'CTAG_G
Cuts at: 2218 2218
Size: 6211
BamHI G'GATC_C
Cuts at: 3541 3908 3541
Size: 367 5844
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2039 3122 3532 4987 618
Size: 325 217 627 252 1083 410 1455 1842
Fragments arranged by size:
1842 1455 1083 627 410 325 252 217
BanII G_rGCy'C
Cuts at: 1391 2561 3430 3530 1391
Size: 1170 869 100 4072
Fragments arranged by size:
4072 1170 869 100
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2130 928
Size: 971 231 5009
Fragments arranged by size:
5009 971 231
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4402 4436 1903
Size: 34 50 34 2381 34 3678
Fragments arranged by size:
3678 2381 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4103 5313 4103
Size: 1210 5001
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6211
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4822 136
Size: 107 122 71 631 605 3150 1525
Fragments arranged by size:
3150 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2998 6206 2998
Size: 3208 3003
BlpI GC'TnA_GC
Cuts at: 2607 3628 2607
Size: 1021 5190
Bme1580I G_kGCm'C
Cuts at: 1790 2042 3300 4272 5428 1790
Size: 252 1258 972 1156 2573
Fragments arranged by size:
2573 1258 1156 972 252
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6211
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2818 3294 3942
Size: 416 53 83 399 24 1401 476 648
Cuts at: 3942 4900 442
Size: 958 1753
Fragments arranged by size:
1753 1401 958 648 476 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6211
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2203 2203
Size: 6211
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2541 3126 4871 1239
Size: 1302 585 1745 2579
Fragments arranged by size:
2579 1745 1302 585
Bpu10I CC'TnA_GC
Cuts at: 2560 2560
Size: 6211
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2955 4336 5201 5499 2955
Size: 1381 865 298 3667
Fragments arranged by size:
3667 1381 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4874 882
Size: 617 3375 2219
Fragments arranged by size:
3375 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4002 493
Size: 711 2798 2702
Fragments arranged by size:
2798 2702 711
BsaBI GATnn'nnATC
Cuts at: 3247 6205 3247
Size: 2958 3253
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4018 4400 5035 275
Size: 53 83 186 936 2485 382 635 1451
Fragments arranged by size:
2485 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2448 3369 4644 5042 5316 5463 5874 2448
Size: 921 1275 398 274 147 411 2785
Fragments arranged by size:
2785 1275 921 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2320 2350 3246 3276 3320
Size: 30 165 30 917 30 896 30 44
Cuts at: 3320 3350 1178
Size: 30 4039
Fragments arranged by size:
4039 917 896 165 44 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6211
BseYI C'CCAG_C
Cuts at: 3423 5414 5910 3423
Size: 1991 496 3724
Fragments arranged by size:
3724 1991 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2040 2275 1163
Size: 573 304 235 5099
Fragments arranged by size:
5099 573 304 235
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3661 4422 5450 1540
Size: 275 186 1660 761 1028 2301
Fragments arranged by size:
2301 1660 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2561 4272 4357 5428 2561
Size: 1711 85 1071 3344
Fragments arranged by size:
3344 1711 1071 85
BsmI GAATG_Cn'
Cuts at: 2291 3425 3722 3815 2291
Size: 1134 297 93 4687
Fragments arranged by size:
4687 1134 297 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2042 2561 3300 3430 3530 4272 4357
Size: 399 252 519 739 130 100 742 85
Cuts at: 4357 5428 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 739 519 399 252 130
100 85
BspEI T'CCGG_A
Cuts at: 5874 5874
Size: 6211
BspHI T'CATG_A
Cuts at: 2848 3286 3411 4100 5830 2848
Size: 438 125 689 1730 3229
Fragments arranged by size:
3229 1730 689 438 125
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3566 844
Size: 2722 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4098 1394
Size: 429 2275 3507
Fragments arranged by size:
3507 2275 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2787 4706 4880 65
Size: 1183 1539 1919 174 1396
Fragments arranged by size:
1919 1539 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3369 4855 5042 1700
Size: 110 1559 1486 187 2869
Fragments arranged by size:
2869 1559 1486 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6211
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6211
BssSI C'ACGA_G
Cuts at: 4271 5283 4271
Size: 1012 5199
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6211
BstBI TT'CG_AA
Cuts at: 5015 5015
Size: 6211
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6211
BstXI CCAn_nnnn'nTGG
Cuts at: 3372 3372
Size: 6211
BstYI r'GATC_y
Cuts at: 1972 2998 3541 3908 4293 4310 5762 5848 5860
Size: 1026 543 367 385 17 1452 86 12
Cuts at: 5860 6206 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1026 543 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5659
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5472
Fragments arranged by size:
5472 683 56
BtsI GCAGTG_nn'
Cuts at: 2330 3385 3727 4521 4541 2330
Size: 1055 342 794 20 4000
Fragments arranged by size:
4000 1055 794 342 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6211
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2096 2131 529
Size: 35 1532 35 4609
Fragments arranged by size:
4609 1532 35 35
DraI TTT'AAA
Cuts at: 3515 3515
Size: 6211
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5218 809
Size: 507 3902 1802
Fragments arranged by size:
3902 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3658 4547 6176
Size: 54 1425 50 275 18 1828 889 1629
Cuts at: 6176 8
Size: 43
Fragments arranged by size:
1828 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3658 1537
Size: 275 1846 4090
Fragments arranged by size:
4090 1846 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2172 2929 4141 1353
Size: 819 757 1212 3423
Fragments arranged by size:
3423 1212 819 757
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4798 5172 5318 387
Size: 1064 138 3209 374 146 1280
Fragments arranged by size:
3209 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2559 2559
Size: 6211
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2541 2897 2930 3126 4274 4871 5658
Size: 393 909 356 33 196 1148 597 787
Cuts at: 5658 5926 1239
Size: 268 1524
Fragments arranged by size:
1524 1148 909 787 597 393 356 268
196 33
EcoNI CCTnn'n_nnAGG
Cuts at: 3227 3227
Size: 6211
EcoO109I rG'GnC_Cy
Cuts at: 2814 2814
Size: 6211
EcoRI G'AATT_C
Cuts at: 3520 3898 3520
Size: 378 5833
EcoRV GAT'ATC
Cuts at: 2800 2800
Size: 6211
FspI TGC'GCA
Cuts at: 4717 4717
Size: 6211
HaeII r_GCGC'y
Cuts at: 1678 5358 1678
Size: 3680 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2562 2594 2885 2917 4852
Size: 32 135 32 990 32 291 32 1935
Cuts at: 4852 4884 4926 4958 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1935 990 291 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2134 3097 3555 3801 669
Size: 988 281 196 963 458 246 3079
Fragments arranged by size:
3079 988 963 458 281 246 196
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6211
HpaI GTT'AAC
Cuts at: 3801 3801
Size: 6211
KpnI G_GTAC'C
Cuts at: 3536 3536
Size: 6211
MfeI C'AATT_G
Cuts at: 2280 2985 3810 2280
Size: 705 825 4681
Fragments arranged by size:
4681 825 705
MluI A'CGCG_T
Cuts at: 6087 6087
Size: 6211
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3132 5325 5509 1305
Size: 1827 2193 184 2007
Fragments arranged by size:
2193 2007 1827 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4389
Fragments arranged by size:
4389 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2853 3020 4169 4528 4687 518
Size: 648 1687 167 1149 359 159 2042
Fragments arranged by size:
2042 1687 1149 648 359 167 159
MspA1I CmG'CkG
Cuts at: 1606 2724 3627 4304 5452 5697 1606
Size: 1118 903 677 1148 245 2120
Fragments arranged by size:
2120 1148 1118 903 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5659
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6211
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6211
NotI GC'GGCC_GC
Cuts at: 3658 3658
Size: 6211
NspI r_CATG'y
Cuts at: 1132 1229 3205 3569 5114 1132
Size: 97 1976 364 1545 2229
Fragments arranged by size:
2229 1976 1545 364 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6211
PciI A'CATG_T
Cuts at: 3201 5110 3201
Size: 1909 4302
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6211
PfoI T'CCnGG_A
Cuts at: 2518 2816 2518
Size: 298 5913
PmeI GTTT'AAAC
Cuts at: 3515 3515
Size: 6211
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4280 4312 5793 5825 4280
Size: 32 1481 32 4666
Fragments arranged by size:
4666 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2814 2814
Size: 6211
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6211
PsiI TTA'TAA
Cuts at: 3781 3781
Size: 6211
PspXI vC'TCGA_Gb
Cuts at: 3114 3114
Size: 6211
PstI C_TGCA'G
Cuts at: 830 2797 3563 830
Size: 1967 766 3478
Fragments arranged by size:
3478 1967 766
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6211
PvuII CAG'CTG
Cuts at: 2724 2724
Size: 6211
SacI G_AGCT'C
Cuts at: 2561 2561
Size: 6211
SalI G'TCGA_C
Cuts at: 1936 3553 1936
Size: 1617 4594
SbfI CC_TGCA'GG
Cuts at: 3563 3563
Size: 6211
ScaI AGT'ACT
Cuts at: 2968 4459 6171 2968
Size: 1491 1712 3008
Fragments arranged by size:
3008 1712 1491
SfcI C'TryA_G
Cuts at: 826 1045 2407 2601 2793 3559 4694 5375 5566
Size: 219 1362 194 192 766 1135 681 191
Cuts at: 5566 826
Size: 1471
Fragments arranged by size:
1471 1362 1135 766 681 219 194 192
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6211
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6211
SmaI CCC'GGG
Cuts at: 1872 3538 1872
Size: 1666 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2970 3114 4315 5216 5478 820
Size: 197 926 1027 144 1201 901 262 1553
Fragments arranged by size:
1553 1201 1027 926 901 262 197 144
SnaBI TAC'GTA
Cuts at: 493 4002 493
Size: 3509 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6211
SphI G_CATG'C
Cuts at: 1132 3569 1132
Size: 2437 3774
SspI AAT'ATT
Cuts at: 5 52 2140 4135 5
Size: 47 2088 1995 2081
Fragments arranged by size:
2088 2081 1995 47
StuI AGG'CCT
Cuts at: 1517 2577 1517
Size: 1060 5151
StyI C'CwwG_G
Cuts at: 513 1065 2218 3650 513
Size: 552 1153 1432 3074
Fragments arranged by size:
3074 1432 1153 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4238 4255 4408 4593 5038 325
Size: 1255 2658 17 153 185 445 1498
Fragments arranged by size:
2658 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2063 2966 4457
Size: 274 80 33 51 1443 86 903 1491
Cuts at: 4457 6169 96
Size: 1712 138
Fragments arranged by size:
1712 1491 1443 903 274 138 86 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2065 2097 2245 2277 3246 3278 3320 3352 6171
Size: 32 148 32 969 32 42 32 2819
Cuts at: 6171 6203 2065
Size: 32 2073
Fragments arranged by size:
2819 2073 969 148 42 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2129 2812 1316
Size: 813 683 4715
Fragments arranged by size:
4715 813 683
XbaI T'CTAG_A
Cuts at: 3547 3547
Size: 6211
XhoI C'TCGA_G
Cuts at: 1943 3114 1943
Size: 1171 5040
XmaI C'CCGG_G
Cuts at: 1870 3536 1870
Size: 1666 4545
XmnI GAAnn'nnTTC
Cuts at: 2699 4340 2699
Size: 1641 4570
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4019 276
Size: 53 83 186 936 2485 2468
Fragments arranged by size:
2485 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PsrI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI