Restriction map for
pFN21AB5901
(Circular) (Six-base) MAPSORT of: pFN21AB5901.seq Check: 9421 from: 1 to: 6205
pFN21AB5901 6205 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4015 278
Size: 53 83 186 936 2479 2468
Fragments arranged by size:
2479 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3017 3548 1937
Size: 1080 531 4594
Fragments arranged by size:
4594 1080 531
Acc65I G'GTAC_C
Cuts at: 3526 3526
Size: 6205
AclI AA'CG_TT
Cuts at: 1563 4332 4705 1563
Size: 2769 373 3063
Fragments arranged by size:
3063 2769 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3068 4268 5652 5920 1632
Size: 1436 1200 1384 268 1917
Fragments arranged by size:
1917 1436 1384 1200 268
AflII C'TTAA_G
Cuts at: 820 1017 2873 820
Size: 197 1856 4152
Fragments arranged by size:
4152 1856 197
AflIII A'CryG_T
Cuts at: 1203 5104 6081 1203
Size: 3901 977 1327
Fragments arranged by size:
3901 1327 977
AgeI A'CCGG_T
Cuts at: 5036 5036
Size: 6205
AhdI GACnn_n'nnGTC
Cuts at: 2744 4934 2744
Size: 2190 4015
AleI CACnn'nnGTG
Cuts at: 1166 2650 3062 1166
Size: 1484 412 4309
Fragments arranged by size:
4309 1484 412
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6205
AlwNI CAG_nnn'CTG
Cuts at: 2768 3948 5520 2768
Size: 1180 1572 3453
Fragments arranged by size:
3453 1572 1180
ApaI G_GGCC'C
Cuts at: 3041 3041
Size: 6205
ApaLI G'TGCA_C
Cuts at: 4262 5418 4262
Size: 1156 5049
ApoI r'AATT_y
Cuts at: 1493 2066 2705 3133 3145 3463 3514 3740 3892
Size: 573 639 428 12 318 51 226 152
Cuts at: 3892 6001 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 639 573 428 318 226 152
51 12
AseI AT'TA_AT
Cuts at: 160 1032 4759 160
Size: 872 3727 1606
Fragments arranged by size:
3727 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6205
AvaI C'yCGr_G
Cuts at: 1870 1943 3530 1870
Size: 73 1587 4545
Fragments arranged by size:
4545 1587 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3483 3516 3483
Size: 33 6172
BamHI G'GATC_C
Cuts at: 3535 3902 3535
Size: 367 5838
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3406 3526 4981 618
Size: 325 217 627 1619 120 1455 1842
Fragments arranged by size:
1842 1619 1455 627 325 217 120
BanII G_rGCy'C
Cuts at: 1391 2024 3041 3524 1391
Size: 633 1017 483 4072
Fragments arranged by size:
4072 1017 633 483
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5234
BbvCI CC'TCA_GC
Cuts at: 2474 2474
Size: 6205
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4396 4430 1903
Size: 34 2459 34 3678
Fragments arranged by size:
3678 2459 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2872 3511 4097 5307 2872
Size: 639 586 1210 3770
Fragments arranged by size:
3770 1210 639 586
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6205
BfrBI ATG'CAT
Cuts at: 2544 2544
Size: 6205
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4816 136
Size: 107 122 71 631 605 3144 1525
Fragments arranged by size:
3144 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6200 6200
Size: 6205
BlpI GC'TnA_GC
Cuts at: 3622 3622
Size: 6205
Bme1580I G_kGCm'C
Cuts at: 1790 3041 3411 4266 5422 1790
Size: 1251 370 855 1156 2573
Fragments arranged by size:
2573 1251 1156 855 370
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6205
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3047 3936 4894
Size: 416 53 83 399 24 1630 889 958
Cuts at: 4894 442
Size: 1753
Fragments arranged by size:
1753 1630 958 889 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6205
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4865 1239
Size: 3626 2579
Bpu10I CC'TnA_GC
Cuts at: 2474 2474
Size: 6205
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2892 4330 5195 5493 2892
Size: 1438 865 298 3604
Fragments arranged by size:
3604 1438 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4868 882
Size: 617 3369 2219
Fragments arranged by size:
3369 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3996 493
Size: 711 2792 2702
Fragments arranged by size:
2792 2702 711
BsaBI GATnn'nnATC
Cuts at: 6199 6199
Size: 6205
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4012 4394 5029 275
Size: 53 83 186 936 2479 382 635 1451
Fragments arranged by size:
2479 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4638 5036 5310 5457 5868 4638
Size: 398 274 147 411 4975
Fragments arranged by size:
4975 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5980
Fragments arranged by size:
5980 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2048 1186
Size: 862 5343
BseYI C'CCAG_C
Cuts at: 5408 5904 5408
Size: 496 5709
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3390 1163
Size: 573 1654 3978
Fragments arranged by size:
3978 1654 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3655 4416 5444 1540
Size: 275 186 1654 761 1028 2301
Fragments arranged by size:
2301 1654 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2762 4266 4351 5422 2762
Size: 1504 85 1071 3545
Fragments arranged by size:
3545 1504 1071 85
BsmI GAATG_Cn'
Cuts at: 3233 3716 3809 3233
Size: 483 93 5629
Fragments arranged by size:
5629 483 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 2762 3041 3411 3524 4266 4351
Size: 399 234 738 279 370 113 742 85
Cuts at: 4351 5422 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 738 399 370 279 234
113 85
BspEI T'CCGG_A
Cuts at: 5868 5868
Size: 6205
BspHI T'CATG_A
Cuts at: 4094 5824 4094
Size: 1730 4475
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3560 844
Size: 2716 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2077 4092 1394
Size: 429 254 2015 3507
Fragments arranged by size:
3507 2015 429 254
BsrDI GCAATG_nn'
Cuts at: 65 1248 2544 3117 3361 4700 4874 65
Size: 1183 1296 573 244 1339 174 1396
Fragments arranged by size:
1396 1339 1296 1183 573 244 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4849 5036 1700
Size: 110 3039 187 2869
Fragments arranged by size:
3039 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3186 96
Size: 3090 3115
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6205
BssSI C'ACGA_G
Cuts at: 4265 5277 4265
Size: 1012 5193
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2168 794
Size: 1374 4831
BstBI TT'CG_AA
Cuts at: 5009 5009
Size: 6205
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6205
BstYI r'GATC_y
Cuts at: 1972 3535 3902 4287 4304 5756 5842 5854 6200
Size: 1563 367 385 17 1452 86 12 346
Cuts at: 6200 1972
Size: 1977
Fragments arranged by size:
1977 1563 1452 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 3018 3018
Size: 6205
Bsu36I CC'TnA_GG
Cuts at: 2551 2551
Size: 6205
BtgI C'CryG_G
Cuts at: 513 1065 2004 2028 3353 513
Size: 552 939 24 1325 3365
Fragments arranged by size:
3365 1325 939 552 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2040 2286 487
Size: 683 56 814 246 4406
Fragments arranged by size:
4406 814 683 246 56
BtsI GCAGTG_nn'
Cuts at: 2242 2657 3373 3721 4515 4535 2242
Size: 415 716 348 794 20 3912
Fragments arranged by size:
3912 794 716 415 348 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6205
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6170
DraI TTT'AAA
Cuts at: 2124 3509 2124
Size: 1385 4820
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5212 809
Size: 507 3896 1802
Fragments arranged by size:
3896 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3356 3652 4541
Size: 54 1425 50 275 18 1526 296 889
Cuts at: 4541 6170 8
Size: 1629 43
Fragments arranged by size:
1629 1526 1425 889 296 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3652 1537
Size: 275 1840 4090
Fragments arranged by size:
4090 1840 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4135 1353
Size: 2782 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4792 5166 5312 387
Size: 1064 138 3203 374 146 1280
Fragments arranged by size:
3203 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3068 4268 4865 5652 5920 1239
Size: 393 1436 1200 597 787 268 1524
Fragments arranged by size:
1524 1436 1200 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 3038 3038
Size: 6205
EcoRI G'AATT_C
Cuts at: 2705 3514 3892 2705
Size: 809 378 5018
Fragments arranged by size:
5018 809 378
FspI TGC'GCA
Cuts at: 2046 4711 2046
Size: 2665 3540
HaeII r_GCGC'y
Cuts at: 1678 2982 5352 1678
Size: 1304 2370 2531
Fragments arranged by size:
2531 2370 1304
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2675 2707 2730 2762 2828
Size: 32 135 32 1103 32 23 32 66
Cuts at: 2828 2860 4846 4878 4920 4952 1373
Size: 32 1986 32 42 32 2626
Fragments arranged by size:
2626 1986 1103 135 66 42 32 32
32 32 32 32 32 23
HincII GTy'rAC
Cuts at: 669 1657 1938 3549 3795 669
Size: 988 281 1611 246 3079
Fragments arranged by size:
3079 1611 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6205
HpaI GTT'AAC
Cuts at: 3795 3795
Size: 6205
KpnI G_GTAC'C
Cuts at: 3530 3530
Size: 6205
MfeI C'AATT_G
Cuts at: 3804 3804
Size: 6205
MluI A'CGCG_T
Cuts at: 6081 6081
Size: 6205
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3227 5319 5503 1305
Size: 1922 2092 184 2007
Fragments arranged by size:
2092 2007 1922 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4383
Fragments arranged by size:
4383 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2465 2650 2859 3062 3366 4163 4522
Size: 648 1299 185 209 203 304 797 359
Cuts at: 4522 4681 518
Size: 159 2042
Fragments arranged by size:
2042 1299 797 648 359 304 209 203
185 159
MspA1I CmG'CkG
Cuts at: 1606 2030 3355 3621 4298 5446 5691 1606
Size: 424 1325 266 677 1148 245 2120
Fragments arranged by size:
2120 1325 1148 677 424 266 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4714
Fragments arranged by size:
4714 939 552
NdeI CA'TA_TG
Cuts at: 387 2182 387
Size: 1795 4410
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6205
NotI GC'GGCC_GC
Cuts at: 3652 3652
Size: 6205
NsiI A_TGCA'T
Cuts at: 2546 2546
Size: 6205
NspI r_CATG'y
Cuts at: 1132 1229 2051 2618 3563 5108 1132
Size: 97 822 567 945 1545 2229
Fragments arranged by size:
2229 1545 945 822 567 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6205
PciI A'CATG_T
Cuts at: 5104 5104
Size: 6205
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2060 1108
Size: 952 5253
PfoI T'CCnGG_A
Cuts at: 2984 2984
Size: 6205
PmeI GTTT'AAAC
Cuts at: 3509 3509
Size: 6205
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4274 4306 5787 5819 4274
Size: 32 1481 32 4660
Fragments arranged by size:
4660 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6205
PsiI TTA'TAA
Cuts at: 3775 3775
Size: 6205
PspOMI G'GGCC_C
Cuts at: 3037 3037
Size: 6205
PstI C_TGCA'G
Cuts at: 830 3557 830
Size: 2727 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6205
SacII CC_GC'GG
Cuts at: 2031 3356 2031
Size: 1325 4880
SalI G'TCGA_C
Cuts at: 1936 3547 1936
Size: 1611 4594
SbfI CC_TGCA'GG
Cuts at: 3557 3557
Size: 6205
ScaI AGT'ACT
Cuts at: 2148 4453 6165 2148
Size: 2305 1712 2188
Fragments arranged by size:
2305 2188 1712
SfcI C'TryA_G
Cuts at: 826 1045 3486 3553 4688 5369 5560 826
Size: 219 2441 67 1135 681 191 1471
Fragments arranged by size:
2441 1471 1135 681 219 191 67
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6205
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6205
SmaI CCC'GGG
Cuts at: 1872 3532 1872
Size: 1660 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2873 2907 4309 5210 5472 820
Size: 197 926 930 34 1402 901 262 1553
Fragments arranged by size:
1553 1402 930 926 901 262 197 34
SnaBI TAC'GTA
Cuts at: 493 3996 493
Size: 3503 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6205
SphI G_CATG'C
Cuts at: 1132 2051 3563 1132
Size: 919 1512 3774
Fragments arranged by size:
3774 1512 919
SspI AAT'ATT
Cuts at: 5 52 4129 5
Size: 47 4077 2081
Fragments arranged by size:
4077 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6205
StyI C'CwwG_G
Cuts at: 513 1065 2004 3644 513
Size: 552 939 1640 3074
Fragments arranged by size:
3074 1640 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4232 4249 4402 4587 5032 325
Size: 1255 2652 17 153 185 445 1498
Fragments arranged by size:
2652 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2146 3186 4451
Size: 274 80 33 51 1443 169 1040 1265
Cuts at: 4451 6163 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1265 1040 274 169 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2056 2088 2547 2579 2926 2958 6165 6197 2056
Size: 32 459 32 347 32 3207 32 2064
Fragments arranged by size:
3207 2064 459 347 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6205
XbaI T'CTAG_A
Cuts at: 3541 3541
Size: 6205
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6205
XmaI C'CCGG_G
Cuts at: 1870 3530 1870
Size: 1660 4545
XmnI GAAnn'nnTTC
Cuts at: 4334 4334
Size: 6205
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4013 276
Size: 53 83 186 936 2479 2468
Fragments arranged by size:
2479 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BplI BsiWI
BsmBI BstXI DraIII EcoICRI EcoNI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PpuMI PspXI PsrI PvuII RsrII SacI SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI