Restriction map for
pFN21AB5897
(Circular) (Six-base) MAPSORT of: pFN21AB5897.seq Check: 4070 from: 1 to: 7216
pFN21AB5897 7216 bp
With 182 enzymes: SgfI *
May 6, 2010 17:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2830 2830
Size: 7216
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5026 278
Size: 53 83 186 936 3490 2468
Fragments arranged by size:
3490 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4071 4559 1937
Size: 2134 488 4594
Fragments arranged by size:
4594 2134 488
Acc65I G'GTAC_C
Cuts at: 4537 4537
Size: 7216
AclI AA'CG_TT
Cuts at: 1563 5343 5716 1563
Size: 3780 373 3063
Fragments arranged by size:
3780 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2045 2094 3171 3208 3818 4205 4349 5279
Size: 413 49 1077 37 610 387 144 930
Cuts at: 5279 6663 6931 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1077 930 610 413 387 268
144 49 37
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7019
AflIII A'CryG_T
Cuts at: 1203 6115 7092 1203
Size: 4912 977 1327
Fragments arranged by size:
4912 1327 977
AgeI A'CCGG_T
Cuts at: 6047 6047
Size: 7216
AhdI GACnn_n'nnGTC
Cuts at: 5945 5945
Size: 7216
AleI CACnn'nnGTG
Cuts at: 1166 4248 1166
Size: 3082 4134
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2426 2439 1184
Size: 1242 13 5961
Fragments arranged by size:
5961 1242 13
AlwNI CAG_nnn'CTG
Cuts at: 2075 2147 2183 3004 4254 4959 6531 2075
Size: 72 36 821 1250 705 1572 2760
Fragments arranged by size:
2760 1572 1250 821 705 72 36
ApaI G_GGCC'C
Cuts at: 2193 2193
Size: 7216
ApaLI G'TGCA_C
Cuts at: 5273 6429 5273
Size: 1156 6060
ApoI r'AATT_y
Cuts at: 1493 3077 4525 4751 4903 7012 1493
Size: 1584 1448 226 152 2109 1697
Fragments arranged by size:
2109 1697 1584 1448 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5770 160
Size: 872 4738 1606
Fragments arranged by size:
4738 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7216
AvaI C'yCGr_G
Cuts at: 1870 1943 2192 2748 3041 3459 4075 4541 1870
Size: 73 249 556 293 418 616 466 4545
Fragments arranged by size:
4545 616 556 466 418 293 249 73
BamHI G'GATC_C
Cuts at: 2227 4546 4913 2227
Size: 2319 367 4530
Fragments arranged by size:
4530 2319 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2137 3037 3901 4537 5992
Size: 325 217 627 350 900 864 636 1455
Cuts at: 5992 618
Size: 1842
Fragments arranged by size:
1842 1455 900 864 636 627 350 325
217
BanII G_rGCy'C
Cuts at: 1391 2193 2775 4083 4535 1391
Size: 802 582 1308 452 4072
Fragments arranged by size:
4072 1308 802 582 452
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2320 3727 4337 928
Size: 971 421 1407 610 3807
Fragments arranged by size:
3807 1407 971 610 421
BbvCI CC'TCA_GC
Cuts at: 2850 2850
Size: 7216
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5407 5441 1903
Size: 34 3470 34 3678
Fragments arranged by size:
3678 3470 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2488 5108 6318 2488
Size: 2620 1210 3386
Fragments arranged by size:
3386 2620 1210
BclI T'GATC_A
Cuts at: 1548 3663 1548
Size: 2115 5101
BfrBI ATG'CAT
Cuts at: 2628 2628
Size: 7216
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3455 5827 136
Size: 107 122 71 631 605 1783 2372 1525
Fragments arranged by size:
2372 1783 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3102 7211 3102
Size: 4109 3107
BlpI GC'TnA_GC
Cuts at: 4082 4633 4082
Size: 551 6665
Bme1580I G_kGCm'C
Cuts at: 1790 2193 2910 3042 3906 5277 6433 1790
Size: 403 717 132 864 1371 1156 2573
Fragments arranged by size:
2573 1371 1156 864 717 403 132
BmgBI CAC'GTC
Cuts at: 1318 2857 1318
Size: 1539 5677
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2193 2821 4242
Size: 416 53 83 399 24 776 628 1421
Cuts at: 4242 4947 5905 442
Size: 705 958 1753
Fragments arranged by size:
1753 1421 958 776 705 628 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7216
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3113 3350 3500 4487 5876 1239
Size: 1874 237 150 987 1389 2579
Fragments arranged by size:
2579 1874 1389 987 237 150
Bpu10I CC'TnA_GC
Cuts at: 2246 2850 2246
Size: 604 6612
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2333 2834 4475 5341 6206 6504 2333
Size: 501 1641 866 865 298 3045
Fragments arranged by size:
3045 1641 866 865 501 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2847 5879 882
Size: 617 1348 3032 2219
Fragments arranged by size:
3032 2219 1348 617
BsaAI yAC'GTr
Cuts at: 493 1204 2476 2905 4366 5007 493
Size: 711 1272 429 1461 641 2702
Fragments arranged by size:
2702 1461 1272 711 641 429
BsaBI GATnn'nnATC
Cuts at: 7210 7210
Size: 7216
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3530 5023 5405 6040
Size: 53 83 186 936 1997 1493 382 635
Cuts at: 6040 275
Size: 1451
Fragments arranged by size:
1997 1493 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2970 5649 6047 6321 6468 6879 2970
Size: 2679 398 274 147 411 3307
Fragments arranged by size:
3307 2679 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2074 2104 2480 2510 1178
Size: 30 165 30 671 30 376 30 5884
Fragments arranged by size:
5884 671 376 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3110 3113 3497 1186
Size: 1924 3 384 4905
Fragments arranged by size:
4905 1924 384 3
BseYI C'CCAG_C
Cuts at: 2068 2099 2152 2164 2521 3209 3230 3421 6419
Size: 31 53 12 357 688 21 191 2998
Cuts at: 6419 6915 2068
Size: 496 2369
Fragments arranged by size:
2998 2369 688 496 357 191 53 31
21 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2079 2379 2741 3216 1163
Size: 573 343 300 362 475 5163
Fragments arranged by size:
5163 573 475 362 343 300
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4666 5427 6455 1540
Size: 275 186 2665 761 1028 2301
Fragments arranged by size:
2665 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2460 2775 5277 5362 6433 2460
Size: 315 2502 85 1071 3243
Fragments arranged by size:
3243 2502 1071 315 85
BsmI GAATG_Cn'
Cuts at: 4134 4727 4820 4134
Size: 593 93 6530
Fragments arranged by size:
6530 593 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3425 3425
Size: 7216
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2193 2460 2775 2910 3042 3906 4083
Size: 399 403 267 315 135 132 864 177
Cuts at: 4083 4535 5277 5362 6433 1391
Size: 452 742 85 1071 2174
Fragments arranged by size:
2174 1071 864 742 452 403 399 315
267 177 135 132 85
BspEI T'CCGG_A
Cuts at: 6879 6879
Size: 7216
BspHI T'CATG_A
Cuts at: 5105 6835 5105
Size: 1730 5486
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2581 2830 3751 4230 4401 4571 844
Size: 1737 249 921 479 171 170 3489
Fragments arranged by size:
3489 1737 921 479 249 171 170
BsrBI CCG'CTC
Cuts at: 1394 1823 2220 2755 4345 5103 1394
Size: 429 397 535 1590 758 3507
Fragments arranged by size:
3507 1590 758 535 429 397
BsrDI GCAATG_nn'
Cuts at: 65 1248 5711 5885 65
Size: 1183 4463 174 1396
Fragments arranged by size:
4463 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2168 2994 3543 5860 6047 1700
Size: 110 358 826 549 2317 187 2869
Fragments arranged by size:
2869 2317 826 549 358 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7216
BssHII G'CGCG_C
Cuts at: 1925 2260 2299 1925
Size: 335 39 6842
Fragments arranged by size:
6842 335 39
BssSI C'ACGA_G
Cuts at: 3563 5276 6288 3563
Size: 1713 1012 4491
Fragments arranged by size:
4491 1713 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2707 794
Size: 1913 5303
BstBI TT'CG_AA
Cuts at: 6020 6020
Size: 7216
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7216
BstXI CCAn_nnnn'nTGG
Cuts at: 3376 4465 3376
Size: 1089 6127
BstYI r'GATC_y
Cuts at: 1972 2227 2407 3102 4059 4110 4546 4913 5298
Size: 255 180 695 957 51 436 367 385
Cuts at: 5298 5315 6767 6853 6865 7211 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 957 695 436 385 367 346
255 180 86 51 17 12
Bsu36I CC'TnA_GG
Cuts at: 3609 3609
Size: 7216
BtgI C'CryG_G
Cuts at: 513 1065 2004 3369 3490 513
Size: 552 939 1365 121 4239
Fragments arranged by size:
4239 1365 939 552 121
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4390 487
Size: 683 56 3164 3313
Fragments arranged by size:
3313 3164 683 56
BtsI GCAGTG_nn'
Cuts at: 2391 4732 5526 5546 2391
Size: 2341 794 20 4061
Fragments arranged by size:
4061 2341 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7216
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7181
DraI TTT'AAA
Cuts at: 4520 4520
Size: 7216
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6223 809
Size: 507 4907 1802
Fragments arranged by size:
4907 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3179 3447 4386
Size: 54 1425 50 275 18 1349 268 939
Cuts at: 4386 4663 5552 7181 8
Size: 277 889 1629 43
Fragments arranged by size:
1629 1425 1349 939 889 277 275 268
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4663 1537
Size: 275 2851 4090
Fragments arranged by size:
4090 2851 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2555 2565 2612 3041 3296 5146 1353
Size: 1202 10 47 429 255 1850 3423
Fragments arranged by size:
3423 1850 1202 429 255 47 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 5803 6177 6323 387
Size: 1064 138 435 3779 374 146 1280
Fragments arranged by size:
3779 1280 1064 435 374 146 138
EcoICRI GAG'CTC
Cuts at: 2773 2773
Size: 7216
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2045 2094 3113 3171 3208 3350 3500
Size: 393 413 49 1019 58 37 142 150
Cuts at: 3500 3818 4205 4349 4487 5279 5876 6663 6931
Size: 318 387 144 138 792 597 787 268
Cuts at: 6931 1239
Size: 1524
Fragments arranged by size:
1524 1019 792 787 597 413 393 387
318 268 150 144 142 138 58 49
37
EcoNI CCTnn'n_nnAGG
Cuts at: 3291 3291
Size: 7216
EcoO109I rG'GnC_Cy
Cuts at: 2023 2189 2661 3051 3425 3777 4189 4288 2023
Size: 166 472 390 374 352 412 99 4951
Fragments arranged by size:
4951 472 412 390 374 352 166 99
EcoRI G'AATT_C
Cuts at: 4525 4903 4525
Size: 378 6838
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2803 2803
Size: 7216
FspI TGC'GCA
Cuts at: 2046 5722 2046
Size: 3676 3540
HaeII r_GCGC'y
Cuts at: 1678 3490 4464 6363 1678
Size: 1812 974 1899 2531
Fragments arranged by size:
2531 1899 1812 974
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2110 2142 2462 2480 2494
Size: 32 135 32 538 32 320 18 14
Cuts at: 2494 2512 5857 5889 5931 5963 1373
Size: 18 3345 32 42 32 2626
Fragments arranged by size:
3345 2626 538 320 135 42 32 32
32 32 32 18 18 14
HincII GTy'rAC
Cuts at: 669 1657 1938 4560 4806 669
Size: 988 281 2622 246 3079
Fragments arranged by size:
3079 2622 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2309 2810 748
Size: 1561 501 5154
Fragments arranged by size:
5154 1561 501
HpaI GTT'AAC
Cuts at: 4806 4806
Size: 7216
KpnI G_GTAC'C
Cuts at: 4541 4541
Size: 7216
MfeI C'AATT_G
Cuts at: 4815 4815
Size: 7216
MluI A'CGCG_T
Cuts at: 7092 7092
Size: 7216
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2111 2147 4263 6330 6514 1305
Size: 806 36 2116 2067 184 2007
Fragments arranged by size:
2116 2067 2007 806 184 36
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5394
Fragments arranged by size:
5394 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3368 3899 4248 5174 5533 5692 518
Size: 648 2202 531 349 926 359 159 2042
Fragments arranged by size:
2202 2042 926 648 531 359 349 159
MspA1I CmG'CkG
Cuts at: 1606 2156 2423 3001 3169 3200 3492 3595 4086
Size: 550 267 578 168 31 292 103 491
Cuts at: 4086 4399 4452 4632 5309 6457 6702 1606
Size: 313 53 180 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 578 550 491 313 292
267 245 180 168 103 53 31
NaeI GCC'GGC
Cuts at: 2170 2996 2170
Size: 826 6390
NcoI C'CATG_G
Cuts at: 513 1065 2004 3369 513
Size: 552 939 1365 4360
Fragments arranged by size:
4360 1365 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7216
NgoMIV G'CCGG_C
Cuts at: 2168 2994 2168
Size: 826 6390
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7216
NotI GC'GGCC_GC
Cuts at: 4663 4663
Size: 7216
NruI TCG'CGA
Cuts at: 4419 4419
Size: 7216
NsiI A_TGCA'T
Cuts at: 2630 2630
Size: 7216
NspI r_CATG'y
Cuts at: 1132 1229 3607 3706 4574 6119 1132
Size: 97 2378 99 868 1545 2229
Fragments arranged by size:
2378 2229 1545 868 99 97
PasI CC'CwG_GG
Cuts at: 1348 2665 3715 1348
Size: 1317 1050 4849
Fragments arranged by size:
4849 1317 1050
PciI A'CATG_T
Cuts at: 6115 6115
Size: 7216
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4254 1108
Size: 3146 4070
PmeI GTTT'AAAC
Cuts at: 4520 4520
Size: 7216
PmlI CAC'GTG
Cuts at: 2905 2905
Size: 7216
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5285 5317 6798 6830 5285
Size: 32 1481 32 5671
Fragments arranged by size:
5671 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2661 3051 3777 4189 2661
Size: 390 726 412 5688
Fragments arranged by size:
5688 726 412 390
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7216
PsiI TTA'TAA
Cuts at: 4786 4786
Size: 7216
PspOMI G'GGCC_C
Cuts at: 2189 2189
Size: 7216
PstI C_TGCA'G
Cuts at: 830 2161 2415 2637 3277 3576 4398 4568 830
Size: 1331 254 222 640 299 822 170 3478
Fragments arranged by size:
3478 1331 822 640 299 254 222 170
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7216
PvuII CAG'CTG
Cuts at: 2156 2423 3001 3169 3595 4086 2156
Size: 267 578 168 426 491 5286
Fragments arranged by size:
5286 578 491 426 267 168
SacI G_AGCT'C
Cuts at: 2775 2775
Size: 7216
SacII CC_GC'GG
Cuts at: 3493 3493
Size: 7216
SalI G'TCGA_C
Cuts at: 1936 4558 1936
Size: 2622 4594
SanDI GG'GwC_CC
Cuts at: 2661 3777 2661
Size: 1116 6100
SbfI CC_TGCA'GG
Cuts at: 4568 4568
Size: 7216
ScaI AGT'ACT
Cuts at: 4445 5464 7176 4445
Size: 1019 1712 4485
Fragments arranged by size:
4485 1712 1019
SfcI C'TryA_G
Cuts at: 826 1045 2157 2411 2633 3273 3572 4394 4564
Size: 219 1112 254 222 640 299 822 170
Cuts at: 4564 5699 6380 6571 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1112 822 681 640 299 254
222 219 191 170
SfiI GGCCn_nnn'nGGCC
Cuts at: 3455 3455
Size: 7216
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7216
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7216
SmaI CCC'GGG
Cuts at: 1872 2194 2750 3461 4077 4543 1872
Size: 322 556 711 616 466 4545
Fragments arranged by size:
4545 711 616 556 466 322
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2312 2813 4490 5320 6221 6483
Size: 197 926 369 501 1677 830 901 262
Cuts at: 6483 820
Size: 1553
Fragments arranged by size:
1677 1553 926 901 830 501 369 262
197
SnaBI TAC'GTA
Cuts at: 493 5007 493
Size: 4514 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7216
SphI G_CATG'C
Cuts at: 1132 3607 4574 1132
Size: 2475 967 3774
Fragments arranged by size:
3774 2475 967
SspI AAT'ATT
Cuts at: 5 52 2695 5140 5
Size: 47 2643 2445 2081
Fragments arranged by size:
2643 2445 2081 47
StuI AGG'CCT
Cuts at: 1517 4503 1517
Size: 2986 4230
StyI C'CwwG_G
Cuts at: 513 1065 2004 2463 3369 3534 3888 4655 513
Size: 552 939 459 906 165 354 767 3074
Fragments arranged by size:
3074 939 906 767 552 459 354 165
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2113 3227 3347 3728 5243 5260 5413
Size: 1255 533 1114 120 381 1515 17 153
Cuts at: 5413 5598 6043 325
Size: 185 445 1498
Fragments arranged by size:
1515 1498 1255 1114 533 445 381 185
153 120 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2724 4422 4443
Size: 274 80 33 51 1443 747 1698 21
Cuts at: 4443 5462 7174 96
Size: 1019 1712 138
Fragments arranged by size:
1712 1698 1443 1019 747 274 138 80
51 33 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7176 7208 7176
Size: 32 7184
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7216
XbaI T'CTAG_A
Cuts at: 4552 4552
Size: 7216
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7216
XmaI C'CCGG_G
Cuts at: 1870 2192 2748 3459 4075 4541 1870
Size: 322 556 711 616 466 4545
Fragments arranged by size:
4545 711 616 556 466 322
XmnI GAAnn'nnTTC
Cuts at: 2549 4475 5345 2549
Size: 1926 870 4420
Fragments arranged by size:
4420 1926 870
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5024 276
Size: 53 83 186 936 3490 2468
Fragments arranged by size:
3490 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SanDI SbfI ScaI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI
BstZ17I DraIII EcoRV FseI FspAI KasI NarI PacI
PfoI PspXI PsrI RsrII SapI SexAI SfoI SrfI
SwaI XcmI