Restriction map for
pFN21AB5895
(Circular) (Six-base) MAPSORT of: pFN21AB5895.seq Check: 5236 from: 1 to: 6202
pFN21AB5895 6202 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4012 278
Size: 53 83 186 936 2476 2468
Fragments arranged by size:
2476 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3545 1937
Size: 1608 4594
Acc65I G'GTAC_C
Cuts at: 3523 3523
Size: 6202
AclI AA'CG_TT
Cuts at: 1563 4329 4702 1563
Size: 2766 373 3063
Fragments arranged by size:
3063 2766 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2345 2911 3374 4265 5649 5917 1632
Size: 713 566 463 891 1384 268 1917
Fragments arranged by size:
1917 1384 891 713 566 463 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6005
AflIII A'CryG_T
Cuts at: 1203 2214 2518 2520 5101 6078 1203
Size: 1011 304 2 2581 977 1327
Fragments arranged by size:
2581 1327 1011 977 304 2
AgeI A'CCGG_T
Cuts at: 5033 5033
Size: 6202
AhdI GACnn_n'nnGTC
Cuts at: 4931 4931
Size: 6202
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6202
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6202
AlwNI CAG_nnn'CTG
Cuts at: 2938 3945 5517 2938
Size: 1007 1572 3623
Fragments arranged by size:
3623 1572 1007
ApaLI G'TGCA_C
Cuts at: 2514 2852 4259 5415 2514
Size: 338 1407 1156 3301
Fragments arranged by size:
3301 1407 1156 338
ApoI r'AATT_y
Cuts at: 1493 3159 3511 3737 3889 5998 1493
Size: 1666 352 226 152 2109 1697
Fragments arranged by size:
2109 1697 1666 352 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4756 160
Size: 872 3724 1606
Fragments arranged by size:
3724 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6202
AvaI C'yCGr_G
Cuts at: 1870 1943 2676 3527 1870
Size: 73 733 851 4545
Fragments arranged by size:
4545 851 733 73
BamHI G'GATC_C
Cuts at: 3532 3899 3532
Size: 367 5835
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3523 4978 618
Size: 325 217 627 1736 1455 1842
Fragments arranged by size:
1842 1736 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2113 2750 2781 3040 3521 1391
Size: 722 637 31 259 481 4072
Fragments arranged by size:
4072 722 637 481 259 31
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2156 3362 928
Size: 971 257 1206 3768
Fragments arranged by size:
3768 1206 971 257
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2416 2450 2459 2493 2527 2561 4393
Size: 34 479 34 9 34 34 34 1832
Cuts at: 4393 4427 1903
Size: 34 3678
Fragments arranged by size:
3678 1832 479 34 34 34 34 34
34 9
BciVI GTATCCnnnnn_n'
Cuts at: 4094 5304 4094
Size: 1210 4992
BclI T'GATC_A
Cuts at: 1548 3243 3249 1548
Size: 1695 6 4501
Fragments arranged by size:
4501 1695 6
BfrBI ATG'CAT
Cuts at: 2049 2049
Size: 6202
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2424 3342 4813
Size: 107 122 71 631 605 752 918 1471
Cuts at: 4813 136
Size: 1525
Fragments arranged by size:
1525 1471 918 752 631 605 122 107
71
BglII A'GATC_T
Cuts at: 6197 6197
Size: 6202
BlpI GC'TnA_GC
Cuts at: 3619 3619
Size: 6202
Bme1580I G_kGCm'C
Cuts at: 1790 2071 2518 2617 2856 4263 5419 1790
Size: 281 447 99 239 1407 1156 2573
Fragments arranged by size:
2573 1407 1156 447 281 239 99
BmgBI CAC'GTC
Cuts at: 1318 2986 1318
Size: 1668 4534
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3034 3933 4891
Size: 416 53 83 399 24 1617 899 958
Cuts at: 4891 442
Size: 1753
Fragments arranged by size:
1753 1617 958 899 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6202
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2477 2619 4862 1239
Size: 1238 142 2243 2579
Fragments arranged by size:
2579 2243 1238 142
Bpu10I CC'TnA_GC
Cuts at: 2693 2945 2693
Size: 252 5950
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2097 4327 5192 5490 2097
Size: 2230 865 298 2809
Fragments arranged by size:
2809 2230 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4865 882
Size: 617 3366 2219
Fragments arranged by size:
3366 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2521 3993 493
Size: 711 1317 1472 2702
Fragments arranged by size:
2702 1472 1317 711
BsaBI GATnn'nnATC
Cuts at: 2198 2261 3248 6196 2198
Size: 63 987 2948 2204
Fragments arranged by size:
2948 2204 987 63
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3101 4009 4391 5026
Size: 53 83 186 936 1568 908 382 635
Cuts at: 5026 275
Size: 1451
Fragments arranged by size:
1568 1451 936 908 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2209 2448 4635 5033 5307 5454 5865 2209
Size: 239 2187 398 274 147 411 2546
Fragments arranged by size:
2546 2187 411 398 274 239 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2732 2762 1178
Size: 30 165 30 1329 30 4618
Fragments arranged by size:
4618 1329 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2230 1186
Size: 1044 5158
BseYI C'CCAG_C
Cuts at: 2340 2618 2796 2974 3384 5405 5901 2340
Size: 278 178 178 410 2021 496 2641
Fragments arranged by size:
2641 2021 496 410 278 178 178
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2861 3294 1163
Size: 573 1125 433 4071
Fragments arranged by size:
4071 1125 573 433
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2849 3652 4413 5441 1540
Size: 275 186 848 803 761 1028 2301
Fragments arranged by size:
2301 1028 848 803 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2113 2518 2685 2781 2856 3326 4263 4348 5419
Size: 405 167 96 75 470 937 85 1071
Cuts at: 5419 2113
Size: 2896
Fragments arranged by size:
2896 1071 937 470 405 167 96 85
75
BsmI GAATG_Cn'
Cuts at: 3713 3806 3713
Size: 93 6109
BsmBI CGTCTCn'nnnn_
Cuts at: 3092 3092
Size: 6202
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2071 2113 2518 2617 2685 2750 2781
Size: 399 281 42 405 99 68 65 31
Cuts at: 2781 2856 3040 3326 3521 4263 4348 5419 1391
Size: 75 184 286 195 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 405 399 286 281 195
184 99 85 75 68 65 42 31
BspEI T'CCGG_A
Cuts at: 2209 5865 2209
Size: 3656 2546
BspHI T'CATG_A
Cuts at: 3246 4091 5821 3246
Size: 845 1730 3627
Fragments arranged by size:
3627 1730 845
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3557 844
Size: 2713 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4089 1394
Size: 429 2266 3507
Fragments arranged by size:
3507 2266 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4697 4871 65
Size: 1183 3449 174 1396
Fragments arranged by size:
3449 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2335 2534 2882 4846 5033 1700
Size: 110 525 199 348 1964 187 2869
Fragments arranged by size:
2869 1964 525 348 199 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6202
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6202
BssSI C'ACGA_G
Cuts at: 2840 4262 5274 2840
Size: 1422 1012 3768
Fragments arranged by size:
3768 1422 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6202
BstBI TT'CG_AA
Cuts at: 2197 5006 2197
Size: 2809 3393
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6202
BstXI CCAn_nnnn'nTGG
Cuts at: 2038 2653 3172 2038
Size: 615 519 5068
Fragments arranged by size:
5068 615 519
BstYI r'GATC_y
Cuts at: 1972 2301 3075 3532 3899 4284 4301 5753 5839
Size: 329 774 457 367 385 17 1452 86
Cuts at: 5839 5851 6197 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 774 457 385 367 346 329
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2266 2589 2999 3317 513
Size: 552 939 262 323 410 318 3398
Fragments arranged by size:
3398 939 552 410 323 318 262
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2383 487
Size: 683 56 1157 4306
Fragments arranged by size:
4306 1157 683 56
BtsI GCAGTG_nn'
Cuts at: 3282 3718 4512 4532 3282
Size: 436 794 20 4952
Fragments arranged by size:
4952 794 436 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6202
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6167
DraI TTT'AAA
Cuts at: 3506 3506
Size: 6202
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5209 809
Size: 507 3893 1802
Fragments arranged by size:
3893 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2316 3649 4538
Size: 54 1425 50 275 18 486 1333 889
Cuts at: 4538 6167 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1333 889 486 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3649 1537
Size: 275 1837 4090
Fragments arranged by size:
4090 1837 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2101 2448 3026 4132 1353
Size: 748 347 578 1106 3423
Fragments arranged by size:
3423 1106 748 578 347
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2978 4789 5163 5309 387
Size: 1064 138 1389 1811 374 146 1280
Fragments arranged by size:
1811 1389 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2111 2779 2111
Size: 668 5534
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2345 2477 2619 2911 3374 4265 4862
Size: 393 713 132 142 292 463 891 597
Cuts at: 4862 5649 5917 1239
Size: 787 268 1524
Fragments arranged by size:
1524 891 787 713 597 463 393 292
268 142 132
EcoO109I rG'GnC_Cy
Cuts at: 2594 2935 2594
Size: 341 5861
EcoRI G'AATT_C
Cuts at: 3511 3889 3511
Size: 378 5824
EcoRV GAT'ATC
Cuts at: 3232 3232
Size: 6202
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3037 3037
Size: 6202
FspI TGC'GCA
Cuts at: 4708 4708
Size: 6202
HaeII r_GCGC'y
Cuts at: 1678 5349 1678
Size: 3671 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2148 2180 4843 4875 4917
Size: 32 135 32 576 32 2663 32 42
Cuts at: 4917 4949 1373
Size: 32 2626
Fragments arranged by size:
2663 2626 576 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3546 3792 669
Size: 988 281 1608 246 3079
Fragments arranged by size:
3079 1608 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6202
HpaI GTT'AAC
Cuts at: 3792 3792
Size: 6202
KpnI G_GTAC'C
Cuts at: 3527 3527
Size: 6202
MfeI C'AATT_G
Cuts at: 3801 3801
Size: 6202
MluI A'CGCG_T
Cuts at: 6078 6078
Size: 6202
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5316 5500 1305
Size: 4011 184 2007
Fragments arranged by size:
4011 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4380
Fragments arranged by size:
4380 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4160 4519 4678 518
Size: 648 2994 359 159 2042
Fragments arranged by size:
2994 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2591 2964 3001 3618 4295 5443 5688 1606
Size: 985 373 37 617 677 1148 245 2120
Fragments arranged by size:
2120 1148 985 677 617 373 245 37
NaeI GCC'GGC
Cuts at: 2337 2536 2884 2337
Size: 199 348 5655
Fragments arranged by size:
5655 348 199
NcoI C'CATG_G
Cuts at: 513 1065 2004 3317 513
Size: 552 939 1313 3398
Fragments arranged by size:
3398 1313 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6202
NgoMIV G'CCGG_C
Cuts at: 2335 2534 2882 2335
Size: 199 348 5655
Fragments arranged by size:
5655 348 199
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6202
NotI GC'GGCC_GC
Cuts at: 3649 3649
Size: 6202
NsiI A_TGCA'T
Cuts at: 2051 2051
Size: 6202
NspI r_CATG'y
Cuts at: 1132 1229 3560 5105 1132
Size: 97 2331 1545 2229
Fragments arranged by size:
2331 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6202
PciI A'CATG_T
Cuts at: 5101 5101
Size: 6202
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3291 1108
Size: 2183 4019
PmeI GTTT'AAAC
Cuts at: 3506 3506
Size: 6202
PmlI CAC'GTG
Cuts at: 2521 2521
Size: 6202
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4271 4303 5784 5816 4271
Size: 32 1481 32 4657
Fragments arranged by size:
4657 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2594 2594
Size: 6202
PshAI GACnn'nnGTC
Cuts at: 1613 2013 1613
Size: 400 5802
PsiI TTA'TAA
Cuts at: 3772 3772
Size: 6202
PstI C_TGCA'G
Cuts at: 830 3554 830
Size: 2724 3478
PvuI CG_AT'CG
Cuts at: 2001 2849 2001
Size: 848 5354
SacI G_AGCT'C
Cuts at: 2113 2781 2113
Size: 668 5534
SacII CC_GC'GG
Cuts at: 2592 3002 2592
Size: 410 5792
SalI G'TCGA_C
Cuts at: 1936 3544 1936
Size: 1608 4594
SapI GCTCTTCn'nnn_
Cuts at: 2101 2448 3026 2101
Size: 347 578 5277
Fragments arranged by size:
5277 578 347
SbfI CC_TGCA'GG
Cuts at: 3554 3554
Size: 6202
ScaI AGT'ACT
Cuts at: 4450 6162 4450
Size: 1712 4490
SfcI C'TryA_G
Cuts at: 826 1045 2782 3550 4685 5366 5557 826
Size: 219 1737 768 1135 681 191 1471
Fragments arranged by size:
1737 1471 1135 768 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6202
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6202
SmaI CCC'GGG
Cuts at: 1872 2678 3529 1872
Size: 806 851 4545
Fragments arranged by size:
4545 851 806
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2112 4306 5207 5469 820
Size: 197 926 169 2194 901 262 1553
Fragments arranged by size:
2194 1553 926 901 262 197 169
SnaBI TAC'GTA
Cuts at: 493 3993 493
Size: 3500 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6202
SphI G_CATG'C
Cuts at: 1132 3560 1132
Size: 2428 3774
SspI AAT'ATT
Cuts at: 5 52 4126 5
Size: 47 4074 2081
Fragments arranged by size:
4074 2081 47
StuI AGG'CCT
Cuts at: 1517 3345 1517
Size: 1828 4374
StyI C'CwwG_G
Cuts at: 513 1065 2004 2071 3317 3641 513
Size: 552 939 67 1246 324 3074
Fragments arranged by size:
3074 1246 939 552 324 67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2501 2834 4229 4246 4399 4584 5029
Size: 1255 921 333 1395 17 153 185 445
Cuts at: 5029 325
Size: 1498
Fragments arranged by size:
1498 1395 1255 921 445 333 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4448 6160 96
Size: 274 80 33 51 1443 2471 1712 138
Fragments arranged by size:
2471 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6162 6194 6162
Size: 32 6170
Tth111I GACn'n_nGTC
Cuts at: 1316 3299 1316
Size: 1983 4219
XbaI T'CTAG_A
Cuts at: 3538 3538
Size: 6202
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6202
XmaI C'CCGG_G
Cuts at: 1870 2676 3527 1870
Size: 806 851 4545
Fragments arranged by size:
4545 851 806
XmnI GAAnn'nnTTC
Cuts at: 2924 4331 2924
Size: 1407 4795
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4010 276
Size: 53 83 186 936 2476 2468
Fragments arranged by size:
2476 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
SacI SacII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BstZ17I Bsu36I DraIII EcoNI FseI
FspAI KasI NarI NruI PacI PfoI PspOMI PspXI
PsrI PvuII RsrII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI