Restriction map for
pFN21AB5894
(Circular) (Six-base) MAPSORT of: pFN21AB5894.seq Check: 9192 from: 1 to: 6277
pFN21AB5894 6277 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4087 278
Size: 53 83 186 936 2551 2468
Fragments arranged by size:
2551 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3620 1937
Size: 1683 4594
Acc65I G'GTAC_C
Cuts at: 3598 3598
Size: 6277
AclI AA'CG_TT
Cuts at: 1563 4404 4777 1563
Size: 2841 373 3063
Fragments arranged by size:
3063 2841 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2681 4340 5724 5992 1632
Size: 1049 1659 1384 268 1917
Fragments arranged by size:
1917 1659 1384 1049 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6080
AflIII A'CryG_T
Cuts at: 1203 2134 5176 6153 1203
Size: 931 3042 977 1327
Fragments arranged by size:
3042 1327 977 931
AgeI A'CCGG_T
Cuts at: 5108 5108
Size: 6277
AhdI GACnn_n'nnGTC
Cuts at: 5006 5006
Size: 6277
AleI CACnn'nnGTG
Cuts at: 1166 2054 1166
Size: 888 5389
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6277
AlwNI CAG_nnn'CTG
Cuts at: 2704 3572 4020 5592 2704
Size: 868 448 1572 3389
Fragments arranged by size:
3389 1572 868 448
ApaLI G'TGCA_C
Cuts at: 4334 5490 4334
Size: 1156 5121
ApoI r'AATT_y
Cuts at: 1493 2630 3497 3551 3586 3812 3964 6073 1493
Size: 1137 867 54 35 226 152 2109 1697
Fragments arranged by size:
2109 1697 1137 867 226 152 54 35
AseI AT'TA_AT
Cuts at: 160 1032 2823 4831 160
Size: 872 1791 2008 1606
Fragments arranged by size:
2008 1791 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6277
AvaI C'yCGr_G
Cuts at: 1870 1943 3602 1870
Size: 73 1659 4545
Fragments arranged by size:
4545 1659 73
AvrII C'CTAG_G
Cuts at: 3556 3556
Size: 6277
BamHI G'GATC_C
Cuts at: 3607 3974 3607
Size: 367 5910
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3598 5053 618
Size: 325 217 627 1811 1455 1842
Fragments arranged by size:
1842 1811 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2422 3596 1391
Size: 1031 1174 4072
Fragments arranged by size:
4072 1174 1031
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5306
BbvCI CC'TCA_GC
Cuts at: 2250 2250
Size: 6277
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4468 4502 1903
Size: 34 2531 34 3678
Fragments arranged by size:
3678 2531 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2317 4169 5379 2317
Size: 1852 1210 3215
Fragments arranged by size:
3215 1852 1210
BclI T'GATC_A
Cuts at: 1548 2094 1548
Size: 546 5731
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4888 136
Size: 107 122 71 631 605 3216 1525
Fragments arranged by size:
3216 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6272 6272
Size: 6277
BlpI GC'TnA_GC
Cuts at: 2943 3517 3694 2943
Size: 574 177 5526
Fragments arranged by size:
5526 574 177
Bme1580I G_kGCm'C
Cuts at: 1790 4338 5494 1790
Size: 2548 1156 2573
Fragments arranged by size:
2573 2548 1156
BmgBI CAC'GTC
Cuts at: 1318 3299 1318
Size: 1981 4296
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2034 4008 4966
Size: 416 53 83 399 24 617 1974 958
Cuts at: 4966 442
Size: 1753
Fragments arranged by size:
1974 1753 958 617 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6277
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3435 4937 1239
Size: 2196 1502 2579
Fragments arranged by size:
2579 2196 1502
Bpu10I CC'TnA_GC
Cuts at: 2250 2250
Size: 6277
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4402 5267 5565 4402
Size: 865 298 5114
Fragments arranged by size:
5114 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4940 882
Size: 617 3441 2219
Fragments arranged by size:
3441 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4068 493
Size: 711 2864 2702
Fragments arranged by size:
2864 2702 711
BsaBI GATnn'nnATC
Cuts at: 3212 6271 3212
Size: 3059 3218
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4084 4466 5101 275
Size: 53 83 186 936 2551 382 635 1451
Fragments arranged by size:
2551 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2954 4710 5108 5382 5529 5940 2954
Size: 1756 398 274 147 411 3291
Fragments arranged by size:
3291 1756 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6052
Fragments arranged by size:
6052 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6277
BseYI C'CCAG_C
Cuts at: 2059 5480 5976 2059
Size: 3421 496 2360
Fragments arranged by size:
3421 2360 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5704
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3727 4488 5516 1540
Size: 275 186 1726 761 1028 2301
Fragments arranged by size:
2301 1726 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2422 2566 3423 4338 4423 5494 2422
Size: 144 857 915 85 1071 3205
Fragments arranged by size:
3205 1071 915 857 144 85
BsmI GAATG_Cn'
Cuts at: 2601 3323 3788 3881 2601
Size: 722 465 93 4997
Fragments arranged by size:
4997 722 465 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2422 2566 3423 3596 4338 4423 5494
Size: 399 632 144 857 173 742 85 1071
Cuts at: 5494 1391
Size: 2174
Fragments arranged by size:
2174 1071 857 742 632 399 173 144
85
BspEI T'CCGG_A
Cuts at: 2954 5940 2954
Size: 2986 3291
BspHI T'CATG_A
Cuts at: 4166 5896 4166
Size: 1730 4547
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3347 3632 844
Size: 2503 285 3489
Fragments arranged by size:
3489 2503 285
BsrBI CCG'CTC
Cuts at: 1394 1823 2552 4164 1394
Size: 429 729 1612 3507
Fragments arranged by size:
3507 1612 729 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3372 4772 4946 65
Size: 1183 2124 1400 174 1396
Fragments arranged by size:
2124 1400 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4921 5108 1700
Size: 110 3111 187 2869
Fragments arranged by size:
3111 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6277
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6277
BssSI C'ACGA_G
Cuts at: 4337 5349 4337
Size: 1012 5265
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2665 3320 794
Size: 1871 655 3751
Fragments arranged by size:
3751 1871 655
BstBI TT'CG_AA
Cuts at: 5081 5081
Size: 6277
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6277
BstXI CCAn_nnnn'nTGG
Cuts at: 2244 2244
Size: 6277
BstYI r'GATC_y
Cuts at: 1972 3607 3974 4359 4376 5828 5914 5926 6272
Size: 1635 367 385 17 1452 86 12 346
Cuts at: 6272 1972
Size: 1977
Fragments arranged by size:
1977 1635 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5725
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5538
Fragments arranged by size:
5538 683 56
BtsI GCAGTG_nn'
Cuts at: 2049 3060 3793 4587 4607 2049
Size: 1011 733 794 20 3719
Fragments arranged by size:
3719 1011 794 733 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6277
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6242
DraI TTT'AAA
Cuts at: 3451 3581 3451
Size: 130 6147
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5284 809
Size: 507 3968 1802
Fragments arranged by size:
3968 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2586 3724 4613
Size: 54 1425 50 275 18 756 1138 889
Cuts at: 4613 6242 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1138 889 756 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3724 1537
Size: 275 1912 4090
Fragments arranged by size:
4090 1912 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2250 2891 3503 4207 1353
Size: 897 641 612 704 3423
Fragments arranged by size:
3423 897 704 641 612
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4864 5238 5384 387
Size: 1064 138 3275 374 146 1280
Fragments arranged by size:
3275 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2420 2420
Size: 6277
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2681 3435 4340 4937 5724 5992 1239
Size: 393 1049 754 905 597 787 268 1524
Fragments arranged by size:
1524 1049 905 787 754 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2028 2028
Size: 6277
EcoRI G'AATT_C
Cuts at: 3497 3586 3964 3497
Size: 89 378 5810
Fragments arranged by size:
5810 378 89
FspI TGC'GCA
Cuts at: 4783 4783
Size: 6277
HaeII r_GCGC'y
Cuts at: 1678 3045 5424 1678
Size: 1367 2379 2531
Fragments arranged by size:
2531 2379 1367
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2608 2640 3090 3122 4918
Size: 32 135 32 1036 32 450 32 1796
Cuts at: 4918 4950 4992 5024 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1796 1036 450 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3621 3867 669
Size: 988 281 1683 246 3079
Fragments arranged by size:
3079 1683 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2779 748
Size: 2031 4246
HpaI GTT'AAC
Cuts at: 3867 3867
Size: 6277
KpnI G_GTAC'C
Cuts at: 3602 3602
Size: 6277
MfeI C'AATT_G
Cuts at: 3331 3876 3331
Size: 545 5732
MluI A'CGCG_T
Cuts at: 6153 6153
Size: 6277
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2395 2570 5391 5575 1305
Size: 1090 175 2821 184 2007
Fragments arranged by size:
2821 2007 1090 184 175
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2588 10
Size: 54 1425 343 756 3699
Fragments arranged by size:
3699 1425 756 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2054 2090 3149 3155 4235 4594 4753
Size: 648 888 36 1059 6 1080 359 159
Cuts at: 4753 518
Size: 2042
Fragments arranged by size:
2042 1080 1059 888 648 359 159 36
6
MspA1I CmG'CkG
Cuts at: 1606 2270 2584 3693 4370 5518 5763 1606
Size: 664 314 1109 677 1148 245 2120
Fragments arranged by size:
2120 1148 1109 677 664 314 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5725
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6277
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6277
NotI GC'GGCC_GC
Cuts at: 3724 3724
Size: 6277
NspI r_CATG'y
Cuts at: 1132 1229 2138 2939 3635 5180 1132
Size: 97 909 801 696 1545 2229
Fragments arranged by size:
2229 1545 909 801 696 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6277
PciI A'CATG_T
Cuts at: 2134 5176 2134
Size: 3042 3235
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2292 1108
Size: 1184 5093
PmeI GTTT'AAAC
Cuts at: 3581 3581
Size: 6277
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4346 4378 5859 5891 4346
Size: 32 1481 32 4732
Fragments arranged by size:
4732 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2028 2028
Size: 6277
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6277
PsiI TTA'TAA
Cuts at: 2465 3847 2465
Size: 1382 4895
PstI C_TGCA'G
Cuts at: 830 3496 3629 830
Size: 2666 133 3478
Fragments arranged by size:
3478 2666 133
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6277
PvuII CAG'CTG
Cuts at: 2270 2584 2270
Size: 314 5963
SacI G_AGCT'C
Cuts at: 2422 2422
Size: 6277
SalI G'TCGA_C
Cuts at: 1936 3619 1936
Size: 1683 4594
SanDI GG'GwC_CC
Cuts at: 2028 2028
Size: 6277
SapI GCTCTTCn'nnn_
Cuts at: 2891 2891
Size: 6277
SbfI CC_TGCA'GG
Cuts at: 3629 3629
Size: 6277
ScaI AGT'ACT
Cuts at: 4525 6237 4525
Size: 1712 4565
SfcI C'TryA_G
Cuts at: 826 1045 2128 3492 3540 3564 3625 4760 5441
Size: 219 1083 1364 48 24 61 1135 681
Cuts at: 5441 5632 826
Size: 191 1471
Fragments arranged by size:
1471 1364 1135 1083 681 219 191 61
48 24
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6277
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6277
SmaI CCC'GGG
Cuts at: 1872 3604 1872
Size: 1732 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4381 5282 5544 820
Size: 197 926 2438 901 262 1553
Fragments arranged by size:
2438 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4068 493
Size: 3575 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6277
SphI G_CATG'C
Cuts at: 1132 3635 1132
Size: 2503 3774
SspI AAT'ATT
Cuts at: 5 52 4201 5
Size: 47 4149 2081
Fragments arranged by size:
4149 2081 47
StuI AGG'CCT
Cuts at: 1517 2514 1517
Size: 997 5280
StyI C'CwwG_G
Cuts at: 513 1065 2243 3556 3716 513
Size: 552 1178 1313 160 3074
Fragments arranged by size:
3074 1313 1178 552 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2132 2175 2340 4304 4321 4474 4659
Size: 1255 552 43 165 1964 17 153 185
Cuts at: 4659 5104 325
Size: 445 1498
Fragments arranged by size:
1964 1498 1255 552 445 185 165 153
43 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2192 3369 4523
Size: 274 80 33 51 1443 215 1177 1154
Cuts at: 4523 6235 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1177 1154 274 215 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3381 3413 6237 6269 3381
Size: 32 2824 32 3389
Fragments arranged by size:
3389 2824 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3297 1316
Size: 1981 4296
XbaI T'CTAG_A
Cuts at: 3613 3613
Size: 6277
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3509 3509
Size: 6277
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6277
XmaI C'CCGG_G
Cuts at: 1870 3602 1870
Size: 1732 4545
XmnI GAAnn'nnTTC
Cuts at: 3179 4406 3179
Size: 1227 5050
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4085 276
Size: 53 83 186 936 2551 2468
Fragments arranged by size:
2551 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII
SacII SexAI SfiI SfoI SrfI SwaI