Restriction map for
pFN21AB5889
(Circular) (Six-base) MAPSORT of: pFN21AB5889.seq Check: 9067 from: 1 to: 6184
pFN21AB5889 6184 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3488 3488
Size: 6184
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2877 3994 278
Size: 53 83 186 936 1341 1117 2468
Fragments arranged by size:
2468 1341 1117 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2877 3527 1937
Size: 940 650 4594
Fragments arranged by size:
4594 940 650
Acc65I G'GTAC_C
Cuts at: 3505 3505
Size: 6184
AclI AA'CG_TT
Cuts at: 1563 4311 4684 1563
Size: 2748 373 3063
Fragments arranged by size:
3063 2748 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2405 4247 5631 5899 1632
Size: 773 1842 1384 268 1917
Fragments arranged by size:
1917 1842 1384 773 268
AfeI AGC'GCT
Cuts at: 2948 2948
Size: 6184
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5987
AflIII A'CryG_T
Cuts at: 1203 3189 5083 6060 1203
Size: 1986 1894 977 1327
Fragments arranged by size:
1986 1894 1327 977
AgeI A'CCGG_T
Cuts at: 5015 5015
Size: 6184
AhdI GACnn_n'nnGTC
Cuts at: 4913 4913
Size: 6184
AleI CACnn'nnGTG
Cuts at: 1166 3192 1166
Size: 2026 4158
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6184
AlwNI CAG_nnn'CTG
Cuts at: 3927 5499 3927
Size: 1572 4612
ApaI G_GGCC'C
Cuts at: 2571 2571
Size: 6184
ApaLI G'TGCA_C
Cuts at: 3002 3080 3239 3380 4241 5397 3002
Size: 78 159 141 861 1156 3789
Fragments arranged by size:
3789 1156 861 159 141 78
ApoI r'AATT_y
Cuts at: 1493 3493 3719 3871 5980 1493
Size: 2000 226 152 2109 1697
Fragments arranged by size:
2109 2000 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4738 160
Size: 872 3706 1606
Fragments arranged by size:
3706 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6184
AvaI C'yCGr_G
Cuts at: 1870 1943 2067 2668 2815 3451 3509 1870
Size: 73 124 601 147 636 58 4545
Fragments arranged by size:
4545 636 601 147 124 73 58
BamHI G'GATC_C
Cuts at: 3514 3881 3514
Size: 367 5817
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2206 2398 2428 2695 2759
Size: 325 217 627 419 192 30 267 64
Cuts at: 2759 3505 4960 618
Size: 746 1455 1842
Fragments arranged by size:
1842 1455 746 627 419 325 267 217
192 64 30
BanII G_rGCy'C
Cuts at: 1391 2148 2244 2571 3212 3273 3336 3503 1391
Size: 757 96 327 641 61 63 167 4072
Fragments arranged by size:
4072 757 641 327 167 96 63 61
BbeI G_GCGC'C
Cuts at: 2210 2402 2432 2210
Size: 192 30 5962
Fragments arranged by size:
5962 192 30
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2558 928
Size: 971 659 4554
Fragments arranged by size:
4554 971 659
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2677 2711 4375 4409 1903
Size: 34 740 34 1664 34 3678
Fragments arranged by size:
3678 1664 740 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4076 5286 4076
Size: 1210 4974
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6184
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2204 2234 2480
Size: 107 122 71 631 605 532 30 246
Cuts at: 2480 2783 3347 3404 4795 136
Size: 303 564 57 1391 1525
Fragments arranged by size:
1525 1391 631 605 564 532 303 246
122 107 71 57 30
BglII A'GATC_T
Cuts at: 6179 6179
Size: 6184
BlpI GC'TnA_GC
Cuts at: 2142 2726 3206 3601 2142
Size: 584 480 395 4725
Fragments arranged by size:
4725 584 480 395
Bme1580I G_kGCm'C
Cuts at: 1790 2571 2700 2721 2933 3006 3084 3243 3384
Size: 781 129 21 212 73 78 159 141
Cuts at: 3384 4245 5401 1790
Size: 861 1156 2573
Fragments arranged by size:
2573 1156 861 781 212 159 141 129
78 73 21
BmgBI CAC'GTC
Cuts at: 1318 3070 1318
Size: 1752 4432
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3915 4873 442
Size: 416 53 83 399 24 2498 958 1753
Fragments arranged by size:
2498 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6184
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2132 2231 3320 4844 1239
Size: 893 99 1089 1524 2579
Fragments arranged by size:
2579 1524 1089 893 99
Bpu10I CC'TnA_GC
Cuts at: 2702 2711 2702
Size: 9 6175
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4309 5174 5472 4309
Size: 865 298 5021
Fragments arranged by size:
5021 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4847 882
Size: 617 3348 2219
Fragments arranged by size:
3348 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2980 3190 3975 493
Size: 711 1776 210 785 2702
Fragments arranged by size:
2702 1776 785 711 210
BsaBI GATnn'nnATC
Cuts at: 6178 6178
Size: 6184
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2207 2399 2429 2874
Size: 53 83 186 936 674 192 30 445
Cuts at: 2874 3459 3991 4373 5008 275
Size: 585 532 382 635 1451
Fragments arranged by size:
1451 936 674 635 585 532 445 382
192 186 83 53 30
BsaWI w'CCGG_w
Cuts at: 2619 2829 4617 5015 5289 5436 5847 2619
Size: 210 1788 398 274 147 411 2956
Fragments arranged by size:
2956 1788 411 398 274 210 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3005 3035 1178
Size: 30 165 30 1602 30 4327
Fragments arranged by size:
4327 1602 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3185 1186
Size: 1999 4185
BseYI C'CCAG_C
Cuts at: 2053 2721 3399 3465 5387 5883 2053
Size: 668 678 66 1922 496 2354
Fragments arranged by size:
2354 1922 678 668 496 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3394 1163
Size: 573 1658 3953
Fragments arranged by size:
3953 1658 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2238 2307 2439 2517 2532 2659
Size: 275 186 237 69 132 78 15 127
Cuts at: 2659 2811 3050 3634 4395 5423 1540
Size: 152 239 584 761 1028 2301
Fragments arranged by size:
2301 1028 761 584 275 239 237 186
152 132 127 78 69 15
BsiHKAI G_wGCw'C
Cuts at: 2148 2271 3006 3084 3212 3243 3273 3384 4245
Size: 123 735 78 128 31 30 111 861
Cuts at: 4245 4330 5401 2148
Size: 85 1071 2931
Fragments arranged by size:
2931 1071 861 735 128 123 111 85
78 31 30
BsmI GAATG_Cn'
Cuts at: 3695 3788 3695
Size: 93 6091
BsmBI CGTCTCn'nnnn_
Cuts at: 3450 3450
Size: 6184
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2148 2244 2271 2571 2700 2721 2933
Size: 399 358 96 27 300 129 21 212
Cuts at: 2933 3006 3084 3212 3243 3273 3336 3384 3503
Size: 73 78 128 31 30 63 48 119
Cuts at: 3503 4245 4330 5401 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 358 300 212 129
128 119 96 85 78 73 63 48
31 30 27 21
BspEI T'CCGG_A
Cuts at: 2619 2829 5847 2619
Size: 210 3018 2956
Fragments arranged by size:
3018 2956 210
BspHI T'CATG_A
Cuts at: 2085 4073 5803 2085
Size: 1988 1730 2466
Fragments arranged by size:
2466 1988 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2122 3312 3488 3539 844
Size: 1278 1190 176 51 3489
Fragments arranged by size:
3489 1278 1190 176 51
BsrBI CCG'CTC
Cuts at: 1394 1823 2730 4071 1394
Size: 429 907 1341 3507
Fragments arranged by size:
3507 1341 907 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4679 4853 65
Size: 1183 3431 174 1396
Fragments arranged by size:
3431 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2203 2654 2735 2762 2789 3407 4828
Size: 110 393 451 81 27 27 618 1421
Cuts at: 4828 5015 1700
Size: 187 2869
Fragments arranged by size:
2869 1421 618 451 393 187 110 81
27 27
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6184
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6184
BssSI C'ACGA_G
Cuts at: 3167 4244 5256 3167
Size: 1077 1012 4095
Fragments arranged by size:
4095 1077 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3363 3372 794
Size: 2569 9 3606
Fragments arranged by size:
3606 2569 9
BstBI TT'CG_AA
Cuts at: 4988 4988
Size: 6184
BstEII G'GTnAC_C
Cuts at: 1184 2800 1184
Size: 1616 4568
BstYI r'GATC_y
Cuts at: 1972 3514 3881 4266 4283 5735 5821 5833 6179
Size: 1542 367 385 17 1452 86 12 346
Cuts at: 6179 1972
Size: 1977
Fragments arranged by size:
1977 1542 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2106 2106
Size: 6184
BtgI C'CryG_G
Cuts at: 513 1065 2292 2433 2475 2520 2691 3291 3328
Size: 552 1227 141 42 45 171 600 37
Cuts at: 3328 3344 513
Size: 16 3353
Fragments arranged by size:
3353 1227 600 552 171 141 45 42
37 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5445
Fragments arranged by size:
5445 683 56
BtsI GCAGTG_nn'
Cuts at: 2787 3382 3700 4494 4514 2787
Size: 595 318 794 20 4457
Fragments arranged by size:
4457 794 595 318 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6184
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6149
DraI TTT'AAA
Cuts at: 3488 3488
Size: 6184
DraIII CAC_nnn'GTG
Cuts at: 3319 3319
Size: 6184
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5191 809
Size: 507 3875 1802
Fragments arranged by size:
3875 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2235 2304 2436
Size: 54 1425 50 275 18 405 69 132
Cuts at: 2436 2484 2656 2784 3047 3405 3409 3631 4520
Size: 48 172 128 263 358 4 222 889
Cuts at: 4520 6149 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 405 358 275 263 222
172 132 128 69 54 50 48 43
18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2235 2304 2436 2656 3047 3631 1537
Size: 275 423 69 132 220 391 584 4090
Fragments arranged by size:
4090 584 423 391 275 220 132 69
EarI CTCTTCn'nnn_
Cuts at: 1353 4114 1353
Size: 2761 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2278 2397 2449 4771 5145 5291
Size: 1064 138 689 119 52 2322 374 146
Cuts at: 5291 387
Size: 1280
Fragments arranged by size:
2322 1280 1064 689 374 146 138 119
52
EcoICRI GAG'CTC
Cuts at: 2146 3210 3271 2146
Size: 1064 61 5059
Fragments arranged by size:
5059 1064 61
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2132 2231 2405 3320 4247 4844 5631
Size: 393 500 99 174 915 927 597 787
Cuts at: 5631 5899 1239
Size: 268 1524
Fragments arranged by size:
1524 927 915 787 597 500 393 268
174 99
EcoO109I rG'GnC_Cy
Cuts at: 2227 2567 2568 2662 3251 3340 2227
Size: 340 1 94 589 89 5071
Fragments arranged by size:
5071 589 340 94 89 1
EcoRI G'AATT_C
Cuts at: 3493 3871 3493
Size: 378 5806
FseI GG_CCGG'CC
Cuts at: 2658 3411 2658
Size: 753 5431
FspI TGC'GCA
Cuts at: 3308 4690 3308
Size: 1382 4802
HaeII r_GCGC'y
Cuts at: 1678 2210 2402 2432 2775 2950 3000 5331 1678
Size: 532 192 30 343 175 50 2331 2531
Fragments arranged by size:
2531 2331 532 343 192 175 50 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2135 2167 4825 4857 4899
Size: 32 135 32 563 32 2658 32 42
Cuts at: 4899 4931 1373
Size: 32 2626
Fragments arranged by size:
2658 2626 563 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3528 3774 669
Size: 988 281 1590 246 3079
Fragments arranged by size:
3079 1590 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6184
HpaI GTT'AAC
Cuts at: 3774 3774
Size: 6184
KasI G'GCGC_C
Cuts at: 2206 2398 2428 2206
Size: 192 30 5962
Fragments arranged by size:
5962 192 30
KpnI G_GTAC'C
Cuts at: 3509 3509
Size: 6184
MfeI C'AATT_G
Cuts at: 3783 3783
Size: 6184
MluI A'CGCG_T
Cuts at: 6060 6060
Size: 6184
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5298 5482 1305
Size: 3993 184 2007
Fragments arranged by size:
3993 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4362
Fragments arranged by size:
4362 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3192 3236 3264 4142 4501 4660 518
Size: 648 2026 44 28 878 359 159 2042
Fragments arranged by size:
2042 2026 878 648 359 159 44 28
MspA1I CmG'CkG
Cuts at: 1606 2294 2309 2318 2378 2435 2477 2725 3346
Size: 688 15 9 60 57 42 248 621
Cuts at: 3346 3403 3600 4277 5425 5670 1606
Size: 57 197 677 1148 245 2120
Fragments arranged by size:
2120 1148 688 677 621 248 245 197
60 57 57 42 15 9
NaeI GCC'GGC
Cuts at: 2205 2656 2737 2764 3409 2205
Size: 451 81 27 645 4980
Fragments arranged by size:
4980 645 451 81 27
NarI GG'CG_CC
Cuts at: 2207 2399 2429 2207
Size: 192 30 5962
Fragments arranged by size:
5962 192 30
NcoI C'CATG_G
Cuts at: 513 1065 2691 513
Size: 552 1626 4006
Fragments arranged by size:
4006 1626 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6184
NgoMIV G'CCGG_C
Cuts at: 2203 2654 2735 2762 3407 2203
Size: 451 81 27 645 4980
Fragments arranged by size:
4980 645 451 81 27
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6184
NotI GC'GGCC_GC
Cuts at: 2304 3631 2304
Size: 1327 4857
NspI r_CATG'y
Cuts at: 1132 1229 3418 3542 5087 1132
Size: 97 2189 124 1545 2229
Fragments arranged by size:
2229 2189 1545 124 97
PasI CC'CwG_GG
Cuts at: 1348 2170 1348
Size: 822 5362
PciI A'CATG_T
Cuts at: 5083 5083
Size: 6184
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6184
PmeI GTTT'AAAC
Cuts at: 3488 3488
Size: 6184
PmlI CAC'GTG
Cuts at: 2980 3190 2980
Size: 210 5974
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4253 4285 5766 5798 4253
Size: 32 1481 32 4639
Fragments arranged by size:
4639 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2662 3251 2662
Size: 589 5595
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6184
PsiI TTA'TAA
Cuts at: 3754 3754
Size: 6184
PspOMI G'GGCC_C
Cuts at: 2567 2567
Size: 6184
PstI C_TGCA'G
Cuts at: 830 2133 3536 830
Size: 1303 1403 3478
Fragments arranged by size:
3478 1403 1303
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6184
PvuII CAG'CTG
Cuts at: 2725 3403 2725
Size: 678 5506
SacI G_AGCT'C
Cuts at: 2148 3212 3273 2148
Size: 1064 61 5059
Fragments arranged by size:
5059 1064 61
SacII CC_GC'GG
Cuts at: 2295 2436 2478 3347 2295
Size: 141 42 869 5132
Fragments arranged by size:
5132 869 141 42
SalI G'TCGA_C
Cuts at: 1936 3526 1936
Size: 1590 4594
SanDI GG'GwC_CC
Cuts at: 2662 2662
Size: 6184
SbfI CC_TGCA'GG
Cuts at: 2133 3536 2133
Size: 1403 4781
ScaI AGT'ACT
Cuts at: 4432 6144 4432
Size: 1712 4472
SfcI C'TryA_G
Cuts at: 826 1045 2129 3532 4667 5348 5539 826
Size: 219 1084 1403 1135 681 191 1471
Fragments arranged by size:
1471 1403 1135 1084 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2234 2234
Size: 6184
SfoI GGC'GCC
Cuts at: 2208 2400 2430 2208
Size: 192 30 5962
Fragments arranged by size:
5962 192 30
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6184
SgrAI Cr'CCGG_yG
Cuts at: 1700 2203 2789 1700
Size: 503 586 5095
Fragments arranged by size:
5095 586 503
SmaI CCC'GGG
Cuts at: 1872 2069 3453 3511 1872
Size: 197 1384 58 4545
Fragments arranged by size:
4545 1384 197 58
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4288 5189 5451 820
Size: 197 926 2345 901 262 1553
Fragments arranged by size:
2345 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3975 493
Size: 3482 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6184
SphI G_CATG'C
Cuts at: 1132 3542 1132
Size: 2410 3774
SspI AAT'ATT
Cuts at: 5 52 4108 5
Size: 47 4056 2081
Fragments arranged by size:
4056 2081 47
StuI AGG'CCT
Cuts at: 1517 2864 1517
Size: 1347 4837
StyI C'CwwG_G
Cuts at: 513 1065 2541 2592 2649 2691 3623 513
Size: 552 1476 51 57 42 932 3074
Fragments arranged by size:
3074 1476 932 552 57 51 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4211 4228 4381 4566 5011 325
Size: 1255 2631 17 153 185 445 1498
Fragments arranged by size:
2631 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4430 6142 96
Size: 274 80 33 51 1443 2453 1712 138
Fragments arranged by size:
2453 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6144 6176 6144
Size: 32 6152
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6184
XbaI T'CTAG_A
Cuts at: 3520 3520
Size: 6184
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6184
XmaI C'CCGG_G
Cuts at: 1870 2067 3451 3509 1870
Size: 197 1384 58 4545
Fragments arranged by size:
4545 1384 197 58
XmnI GAAnn'nnTTC
Cuts at: 2093 4313 2093
Size: 2220 3964
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2875 3992 276
Size: 53 83 186 936 1341 1117 2468
Fragments arranged by size:
2468 1341 1117 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI
BstXI BstZ17I EcoNI EcoRV FalI FspAI NruI NsiI
PacI PfoI PspXI PsrI RsrII SapI SexAI SrfI
SwaI XcmI