Restriction map for
pFN21AB5887
(Circular) (Six-base) MAPSORT of: pFN21AB5887.seq Check: 8652 from: 1 to: 6196
pFN21AB5887 6196 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4006 278
Size: 53 83 186 936 2470 2468
Fragments arranged by size:
2470 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2318 3539 1937
Size: 381 1221 4594
Fragments arranged by size:
4594 1221 381
Acc65I G'GTAC_C
Cuts at: 3517 3517
Size: 6196
AclI AA'CG_TT
Cuts at: 1563 4323 4696 1563
Size: 2760 373 3063
Fragments arranged by size:
3063 2760 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3336 3450 4259 5643 5911 1632
Size: 1704 114 809 1384 268 1917
Fragments arranged by size:
1917 1704 1384 809 268 114
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5999
AflIII A'CryG_T
Cuts at: 1203 5095 6072 1203
Size: 3892 977 1327
Fragments arranged by size:
3892 1327 977
AgeI A'CCGG_T
Cuts at: 5027 5027
Size: 6196
AhdI GACnn_n'nnGTC
Cuts at: 3407 4925 3407
Size: 1518 4678
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6196
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6196
AlwNI CAG_nnn'CTG
Cuts at: 3939 5511 3939
Size: 1572 4624
ApaLI G'TGCA_C
Cuts at: 4253 5409 4253
Size: 1156 5040
ApoI r'AATT_y
Cuts at: 1493 2069 2323 2749 2758 3192 3483 3505 3731
Size: 576 254 426 9 434 291 22 226
Cuts at: 3731 3883 5992 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 576 434 426 291 254 226
152 22 9
AseI AT'TA_AT
Cuts at: 160 1032 4750 160
Size: 872 3718 1606
Fragments arranged by size:
3718 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6196
AvaI C'yCGr_G
Cuts at: 1870 1943 2467 3521 1870
Size: 73 524 1054 4545
Fragments arranged by size:
4545 1054 524 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2025 2058 2025
Size: 33 6163
BamHI G'GATC_C
Cuts at: 3526 3893 3526
Size: 367 5829
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3330 3517 4972 618
Size: 325 217 627 1543 187 1455 1842
Fragments arranged by size:
1842 1543 1455 627 325 217 187
BanII G_rGCy'C
Cuts at: 1391 3515 1391
Size: 2124 4072
BbeI G_GCGC'C
Cuts at: 3334 3334
Size: 6196
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2254 2952 928
Size: 971 355 698 4172
Fragments arranged by size:
4172 971 698 355
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4387 4421 1903
Size: 34 2450 34 3678
Fragments arranged by size:
3678 2450 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2272 2342 2985 4088 5298 2272
Size: 70 643 1103 1210 3170
Fragments arranged by size:
3170 1210 1103 643 70
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6196
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4807 136
Size: 107 122 71 631 605 3135 1525
Fragments arranged by size:
3135 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3241 6191 3241
Size: 2950 3246
BlpI GC'TnA_GC
Cuts at: 3613 3613
Size: 6196
Bme1580I G_kGCm'C
Cuts at: 1790 4257 5413 1790
Size: 2467 1156 2573
Fragments arranged by size:
2573 2467 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6196
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2442 2934 3927
Size: 416 53 83 399 24 1025 492 993
Cuts at: 3927 4885 442
Size: 958 1753
Fragments arranged by size:
1753 1025 993 958 492 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6196
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4856 1239
Size: 3617 2579
Bpu10I CC'TnA_GC
Cuts at: 3326 3326
Size: 6196
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3068 3245 4321 5186 5484 3068
Size: 177 1076 865 298 3780
Fragments arranged by size:
3780 1076 865 298 177
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4859 882
Size: 617 3360 2219
Fragments arranged by size:
3360 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3987 493
Size: 711 2783 2702
Fragments arranged by size:
2783 2702 711
BsaBI GATnn'nnATC
Cuts at: 6190 6190
Size: 6196
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3331 4003 4385 5020
Size: 53 83 186 936 1798 672 382 635
Cuts at: 5020 275
Size: 1451
Fragments arranged by size:
1798 1451 936 672 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4629 5027 5301 5448 5859 4629
Size: 398 274 147 411 4966
Fragments arranged by size:
4966 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2169 2199 1178
Size: 30 165 30 766 30 5175
Fragments arranged by size:
5175 766 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2931 3065 3356 1186
Size: 1745 134 291 4026
Fragments arranged by size:
4026 1745 291 134
BseYI C'CCAG_C
Cuts at: 5399 5895 5399
Size: 496 5700
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2618 1163
Size: 573 882 4741
Fragments arranged by size:
4741 882 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3646 4407 5435 1540
Size: 275 186 1645 761 1028 2301
Fragments arranged by size:
2301 1645 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4257 4342 5413 4257
Size: 85 1071 5040
Fragments arranged by size:
5040 1071 85
BsmI GAATG_Cn'
Cuts at: 3256 3707 3800 3256
Size: 451 93 5652
Fragments arranged by size:
5652 451 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3515 4257 4342 5413 1391
Size: 399 1725 742 85 1071 2174
Fragments arranged by size:
2174 1725 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5859 5859
Size: 6196
BspHI T'CATG_A
Cuts at: 4085 5815 4085
Size: 1730 4466
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2053 2428 3551 844
Size: 1209 375 1123 3489
Fragments arranged by size:
3489 1209 1123 375
BsrBI CCG'CTC
Cuts at: 1394 1823 4083 1394
Size: 429 2260 3507
Fragments arranged by size:
3507 2260 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4691 4865 65
Size: 1183 3443 174 1396
Fragments arranged by size:
3443 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4840 5027 1700
Size: 110 3030 187 2869
Fragments arranged by size:
3030 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6196
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6196
BssSI C'ACGA_G
Cuts at: 2148 4256 5268 2148
Size: 2108 1012 3076
Fragments arranged by size:
3076 2108 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6196
BstBI TT'CG_AA
Cuts at: 2716 5000 2716
Size: 2284 3912
BstEII G'GTnAC_C
Cuts at: 1184 2588 1184
Size: 1404 4792
BstYI r'GATC_y
Cuts at: 1972 2027 2808 3241 3526 3893 4278 4295 5747
Size: 55 781 433 285 367 385 17 1452
Cuts at: 5747 5833 5845 6191 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 781 433 385 367 346 285
86 55 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2787 513
Size: 552 1722 3922
Fragments arranged by size:
3922 1722 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5457
Fragments arranged by size:
5457 683 56
BtsI GCAGTG_nn'
Cuts at: 2606 3712 4506 4526 2606
Size: 1106 794 20 4276
Fragments arranged by size:
4276 1106 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6196
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6161
DraI TTT'AAA
Cuts at: 3500 3500
Size: 6196
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5203 809
Size: 507 3887 1802
Fragments arranged by size:
3887 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2522 3643 4532
Size: 54 1425 50 275 18 692 1121 889
Cuts at: 4532 6161 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1121 889 692 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3643 1537
Size: 275 1831 4090
Fragments arranged by size:
4090 1831 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2073 3312 4126 1353
Size: 720 1239 814 3423
Fragments arranged by size:
3423 1239 814 720
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4783 5157 5303 387
Size: 1064 138 3194 374 146 1280
Fragments arranged by size:
3194 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3336 3450 4259 4856 5643 5911 1239
Size: 393 1704 114 809 597 787 268 1524
Fragments arranged by size:
1704 1524 809 787 597 393 268 114
EcoNI CCTnn'n_nnAGG
Cuts at: 2392 3338 2392
Size: 946 5250
EcoRI G'AATT_C
Cuts at: 3505 3883 3505
Size: 378 5818
EcoRV GAT'ATC
Cuts at: 3201 3201
Size: 6196
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3306 3306
Size: 6196
FspI TGC'GCA
Cuts at: 4702 4702
Size: 6196
HaeII r_GCGC'y
Cuts at: 1678 3334 5343 1678
Size: 1656 2009 2531
Fragments arranged by size:
2531 2009 1656
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2384 2416 3393 3425 4837
Size: 32 135 32 812 32 977 32 1412
Cuts at: 4837 4869 4911 4943 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1412 977 812 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2842 3439 3540 3786 669
Size: 988 281 904 597 101 246 3079
Fragments arranged by size:
3079 988 904 597 281 246 101
HindIII A'AGCT_T
Cuts at: 748 3347 748
Size: 2599 3597
HpaI GTT'AAC
Cuts at: 2842 3786 2842
Size: 944 5252
KasI G'GCGC_C
Cuts at: 3330 3330
Size: 6196
KpnI G_GTAC'C
Cuts at: 3521 3521
Size: 6196
MfeI C'AATT_G
Cuts at: 3795 3795
Size: 6196
MluI A'CGCG_T
Cuts at: 6072 6072
Size: 6196
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2224 5310 5494 1305
Size: 919 3086 184 2007
Fragments arranged by size:
3086 2007 919 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2524 10
Size: 54 1425 343 692 3682
Fragments arranged by size:
3682 1425 692 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4154 4513 4672 518
Size: 648 2988 359 159 2042
Fragments arranged by size:
2988 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2210 3612 4289 5437 5682 1606
Size: 604 1402 677 1148 245 2120
Fragments arranged by size:
2120 1402 1148 677 604 245
NarI GG'CG_CC
Cuts at: 3331 3331
Size: 6196
NcoI C'CATG_G
Cuts at: 513 1065 2787 513
Size: 552 1722 3922
Fragments arranged by size:
3922 1722 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6196
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6196
NotI GC'GGCC_GC
Cuts at: 3643 3643
Size: 6196
NspI r_CATG'y
Cuts at: 1132 1229 2414 2966 3554 5099 1132
Size: 97 1185 552 588 1545 2229
Fragments arranged by size:
2229 1545 1185 588 552 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6196
PciI A'CATG_T
Cuts at: 5095 5095
Size: 6196
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6196
PmeI GTTT'AAAC
Cuts at: 3500 3500
Size: 6196
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4265 4297 5778 5810 4265
Size: 32 1481 32 4651
Fragments arranged by size:
4651 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3444 1613
Size: 1831 4365
PsiI TTA'TAA
Cuts at: 3766 3766
Size: 6196
PstI C_TGCA'G
Cuts at: 830 2050 3548 830
Size: 1220 1498 3478
Fragments arranged by size:
3478 1498 1220
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6196
PvuII CAG'CTG
Cuts at: 2210 2210
Size: 6196
SalI G'TCGA_C
Cuts at: 1936 3538 1936
Size: 1602 4594
SapI GCTCTTCn'nnn_
Cuts at: 3312 3312
Size: 6196
SbfI CC_TGCA'GG
Cuts at: 3548 3548
Size: 6196
ScaI AGT'ACT
Cuts at: 3313 4444 6156 3313
Size: 1131 1712 3353
Fragments arranged by size:
3353 1712 1131
SfcI C'TryA_G
Cuts at: 826 1045 2046 2211 3544 4679 5360 5551 826
Size: 219 1001 165 1333 1135 681 191 1471
Fragments arranged by size:
1471 1333 1135 1001 681 219 191 165
SfoI GGC'GCC
Cuts at: 3332 3332
Size: 6196
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6196
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6196
SmaI CCC'GGG
Cuts at: 1872 3523 1872
Size: 1651 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3047 3224 4300 5201 5463 820
Size: 197 926 1104 177 1076 901 262 1553
Fragments arranged by size:
1553 1104 1076 926 901 262 197 177
SnaBI TAC'GTA
Cuts at: 493 3987 493
Size: 3494 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6196
SphI G_CATG'C
Cuts at: 1132 3554 1132
Size: 2422 3774
SspI AAT'ATT
Cuts at: 5 52 2713 3028 3376 4120 5
Size: 47 2661 315 348 744 2081
Fragments arranged by size:
2661 2081 744 348 315 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6196
StyI C'CwwG_G
Cuts at: 513 1065 2787 2951 3635 513
Size: 552 1722 164 684 3074
Fragments arranged by size:
3074 1722 684 552 164
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4223 4240 4393 4578 5023 325
Size: 1255 2643 17 153 185 445 1498
Fragments arranged by size:
2643 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2346 3311 4442
Size: 274 80 33 51 1443 369 965 1131
Cuts at: 4442 6154 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1131 965 369 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2169 2201 2926 2958 6156 6188 2169
Size: 32 725 32 3198 32 2177
Fragments arranged by size:
3198 2177 725 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6196
XbaI T'CTAG_A
Cuts at: 3532 3532
Size: 6196
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6196
XmaI C'CCGG_G
Cuts at: 1870 3521 1870
Size: 1651 4545
XmnI GAAnn'nnTTC
Cuts at: 3192 4325 3192
Size: 1133 5063
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4004 276
Size: 53 83 186 936 2470 2468
Fragments arranged by size:
2470 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoO109I FseI FspAI NaeI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SexAI SfiI SrfI SwaI XcmI