Restriction map for
pFN21AB5884
(Circular) (Six-base) MAPSORT of: pFN21AB5884.seq Check: 4117 from: 1 to: 6193
pFN21AB5884 6193 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4003 278
Size: 53 83 186 936 2467 2468
Fragments arranged by size:
2468 2467 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3006 3536 1937
Size: 1069 530 4594
Fragments arranged by size:
4594 1069 530
Acc65I G'GTAC_C
Cuts at: 2560 3514 2560
Size: 954 5239
AclI AA'CG_TT
Cuts at: 1563 4320 4693 1563
Size: 2757 373 3063
Fragments arranged by size:
3063 2757 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3025 4256 5640 5908 1632
Size: 1393 1231 1384 268 1917
Fragments arranged by size:
1917 1393 1384 1231 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5996
AflIII A'CryG_T
Cuts at: 1203 5092 6069 1203
Size: 3889 977 1327
Fragments arranged by size:
3889 1327 977
AgeI A'CCGG_T
Cuts at: 2286 5024 2286
Size: 2738 3455
AhdI GACnn_n'nnGTC
Cuts at: 2926 3307 4922 2926
Size: 381 1615 4197
Fragments arranged by size:
4197 1615 381
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6193
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2801 1184
Size: 1617 4576
AlwNI CAG_nnn'CTG
Cuts at: 3936 5508 3936
Size: 1572 4621
ApaI G_GGCC'C
Cuts at: 2142 2939 3039 3287 2142
Size: 797 100 248 5048
Fragments arranged by size:
5048 797 248 100
ApaLI G'TGCA_C
Cuts at: 2309 4250 5406 2309
Size: 1941 1156 3096
Fragments arranged by size:
3096 1941 1156
ApoI r'AATT_y
Cuts at: 1493 3502 3728 3880 5989 1493
Size: 2009 226 152 2109 1697
Fragments arranged by size:
2109 2009 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4747 160
Size: 872 3715 1606
Fragments arranged by size:
3715 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6193
AvaI C'yCGr_G
Cuts at: 1870 1943 2135 2457 2849 2939 3057 3471 3518
Size: 73 192 322 392 90 118 414 47
Cuts at: 3518 1870
Size: 4545
Fragments arranged by size:
4545 414 392 322 192 118 90 73
47
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3202 3235 3202
Size: 33 6160
BamHI G'GATC_C
Cuts at: 3523 3890 3523
Size: 367 5826
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2246 2327 2486 2560 2810
Size: 325 217 627 459 81 159 74 250
Cuts at: 2810 3025 3514 4969 618
Size: 215 489 1455 1842
Fragments arranged by size:
1842 1455 627 489 459 325 250 217
215 159 81 74
BanII G_rGCy'C
Cuts at: 1391 2015 2142 2234 2771 2939 3039 3164 3205
Size: 624 127 92 537 168 100 125 41
Cuts at: 3205 3267 3287 3512 1391
Size: 62 20 225 4072
Fragments arranged by size:
4072 624 537 225 168 127 125 100
92 62 41 20
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2326 928
Size: 971 427 4795
Fragments arranged by size:
4795 971 427
BbvCI CC'TCA_GC
Cuts at: 2207 2207
Size: 6193
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4384 4418 1903
Size: 34 2447 34 3678
Fragments arranged by size:
3678 2447 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3230 4085 5295 3230
Size: 855 1210 4128
Fragments arranged by size:
4128 1210 855
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6193
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2137 2775 3034
Size: 107 122 71 631 605 465 638 259
Cuts at: 3034 3291 4804 136
Size: 257 1513 1525
Fragments arranged by size:
1525 1513 638 631 605 465 259 257
122 107 71
BglII A'GATC_T
Cuts at: 2607 6188 2607
Size: 3581 2612
BlpI GC'TnA_GC
Cuts at: 2036 2171 2795 3610 2036
Size: 135 624 815 4619
Fragments arranged by size:
4619 815 624 135
Bme1580I G_kGCm'C
Cuts at: 1790 2115 2133 2142 2219 2251 2313 2330 2688
Size: 325 18 9 77 32 62 17 358
Cuts at: 2688 2813 2939 3039 3287 3435 4254 5410 1790
Size: 125 126 100 248 148 819 1156 2573
Fragments arranged by size:
2573 1156 819 358 325 248 148 126
125 100 77 62 32 18 17 9
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6193
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2509 2783 3182
Size: 416 53 83 399 24 1092 274 399
Cuts at: 3182 3924 4882 442
Size: 742 958 1753
Fragments arranged by size:
1753 1092 958 742 416 399 399 274
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6193
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2830 2830
Size: 6193
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2936 2994 3443 4853 1239
Size: 1697 58 449 1410 2579
Fragments arranged by size:
2579 1697 1410 449 58
Bpu10I CC'TnA_GC
Cuts at: 2207 2698 3227 2207
Size: 491 529 5173
Fragments arranged by size:
5173 529 491
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4318 5183 5481 4318
Size: 865 298 5030
Fragments arranged by size:
5030 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4856 882
Size: 617 3357 2219
Fragments arranged by size:
3357 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2257 3168 3984 493
Size: 711 1053 911 816 2702
Fragments arranged by size:
2702 1053 911 816 711
BsaBI GATnn'nnATC
Cuts at: 6187 6187
Size: 6193
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2433 3308 4000 4382
Size: 53 83 186 936 900 875 692 382
Cuts at: 4382 5017 275
Size: 635 1451
Fragments arranged by size:
1451 936 900 875 692 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2286 4626 5024 5298 5445 5856 2286
Size: 2340 398 274 147 411 2623
Fragments arranged by size:
2623 2340 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2273 2303 2667 2697 3009
Size: 30 165 30 870 30 364 30 312
Cuts at: 3009 3039 1178
Size: 30 4332
Fragments arranged by size:
4332 870 364 312 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2224 2486 2954 3008 1186
Size: 1038 262 468 54 4371
Fragments arranged by size:
4371 1038 468 262 54
BseYI C'CCAG_C
Cuts at: 2260 2356 2890 3287 5396 5892 2260
Size: 96 534 397 2109 496 2561
Fragments arranged by size:
2561 2109 534 496 397 96
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2237 2399 2513 2555 2807 1163
Size: 573 501 162 114 42 252 4549
Fragments arranged by size:
4549 573 501 252 162 114 42
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2659 3643 4404 5432 1540
Size: 275 186 658 984 761 1028 2301
Fragments arranged by size:
2301 1028 984 761 658 275 186
BsiHKAI G_wGCw'C
Cuts at: 2015 2076 2286 2313 4254 4339 5410 2015
Size: 61 210 27 1941 85 1071 2798
Fragments arranged by size:
2798 1941 1071 210 85 61 27
BsmI GAATG_Cn'
Cuts at: 3704 3797 3704
Size: 93 6100
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2015 2076 2115 2133 2142 2219 2234
Size: 399 225 61 39 18 9 77 15
Cuts at: 2234 2251 2286 2313 2330 2688 2771 2813 2939
Size: 17 35 27 17 358 83 42 126
Cuts at: 2939 3039 3164 3205 3267 3287 3435 3512 4254
Size: 100 125 41 62 20 148 77 742
Cuts at: 4254 4339 5410 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 358 225 148 126
125 100 85 83 77 77 62 61
42 41 39 35 27 20 18 17
17 15 9
BspEI T'CCGG_A
Cuts at: 5856 5856
Size: 6193
BspHI T'CATG_A
Cuts at: 4082 5812 4082
Size: 1730 4463
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2700 3548 844
Size: 1856 848 3489
Fragments arranged by size:
3489 1856 848
BsrBI CCG'CTC
Cuts at: 1394 1823 2083 3363 4080 1394
Size: 429 260 1280 717 3507
Fragments arranged by size:
3507 1280 717 429 260
BsrDI GCAATG_nn'
Cuts at: 65 1248 2764 4688 4862 65
Size: 1183 1516 1924 174 1396
Fragments arranged by size:
1924 1516 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2286 2435 2738 3319 4837 5024 1700
Size: 110 476 149 303 581 1518 187 2869
Fragments arranged by size:
2869 1518 581 476 303 187 149 110
BsrGI T'GTAC_A
Cuts at: 96 3398 96
Size: 3302 2891
BssHII G'CGCG_C
Cuts at: 1925 2405 1925
Size: 480 5713
BssSI C'ACGA_G
Cuts at: 2443 2681 4253 5265 2443
Size: 238 1572 1012 3371
Fragments arranged by size:
3371 1572 1012 238
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2610 794
Size: 1816 4377
BstBI TT'CG_AA
Cuts at: 4997 4997
Size: 6193
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6193
BstXI CCAn_nnnn'nTGG
Cuts at: 2149 3029 2149
Size: 880 5313
BstYI r'GATC_y
Cuts at: 1972 2607 3523 3890 4275 4292 5744 5830 5842
Size: 635 916 367 385 17 1452 86 12
Cuts at: 5842 6188 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 916 635 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2370 2545 3327 2370
Size: 175 782 5236
Fragments arranged by size:
5236 782 175
BtgI C'CryG_G
Cuts at: 513 1065 2388 2527 2653 2912 3013 513
Size: 552 1323 139 126 259 101 3693
Fragments arranged by size:
3693 1323 552 259 139 126 101
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2332 2661 487
Size: 683 56 1106 329 4019
Fragments arranged by size:
4019 1106 683 329 56
BtsI GCAGTG_nn'
Cuts at: 3709 4503 4523 3709
Size: 794 20 5379
Fragments arranged by size:
5379 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6193
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6158
DraI TTT'AAA
Cuts at: 3497 3497
Size: 6193
DraIII CAC_nnn'GTG
Cuts at: 2533 3190 2533
Size: 657 5536
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5200 809
Size: 507 3884 1802
Fragments arranged by size:
3884 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2223 2421 2524
Size: 54 1425 50 275 18 393 198 103
Cuts at: 2524 2656 3640 4529 6158 8
Size: 132 984 889 1629 43
Fragments arranged by size:
1629 1425 984 889 393 275 198 132
103 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2656 3640 1537
Size: 275 844 984 4090
Fragments arranged by size:
4090 984 844 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2081 2345 4123 1353
Size: 728 264 1778 3423
Fragments arranged by size:
3423 1778 728 264
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2294 3212 4780 5154 5300 387
Size: 1064 138 705 918 1568 374 146 1280
Fragments arranged by size:
1568 1280 1064 918 705 374 146 138
EcoICRI GAG'CTC
Cuts at: 2013 2013
Size: 6193
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2936 2994 3025 3443 4256 4853 5640
Size: 393 1304 58 31 418 813 597 787
Cuts at: 5640 5908 1239
Size: 268 1524
Fragments arranged by size:
1524 1304 813 787 597 418 393 268
58 31
EcoNI CCTnn'n_nnAGG
Cuts at: 2026 2468 3413 2026
Size: 442 945 4806
Fragments arranged by size:
4806 945 442
EcoO109I rG'GnC_Cy
Cuts at: 2047 2461 2519 2936 3036 3114 3283 3284 3334
Size: 414 58 417 100 78 169 1 50
Cuts at: 3334 3372 3418 2047
Size: 38 46 4822
Fragments arranged by size:
4822 417 414 169 100 78 58 50
46 38 1
EcoRI G'AATT_C
Cuts at: 3502 3880 3502
Size: 378 5815
FspI TGC'GCA
Cuts at: 4699 4699
Size: 6193
HaeII r_GCGC'y
Cuts at: 1678 2457 2671 2795 5340 1678
Size: 779 214 124 2545 2531
Fragments arranged by size:
2545 2531 779 214 124
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2273 2305 2608 2640 2912
Size: 32 135 32 701 32 303 32 272
Cuts at: 2912 2944 3098 3130 3293 3325 4834 4866 4908
Size: 32 154 32 163 32 1509 32 42
Cuts at: 4908 4940 1373
Size: 32 2626
Fragments arranged by size:
2626 1509 701 303 272 163 154 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3537 3783 669
Size: 988 281 1599 246 3079
Fragments arranged by size:
3079 1599 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6193
HpaI GTT'AAC
Cuts at: 3783 3783
Size: 6193
KpnI G_GTAC'C
Cuts at: 2564 3518 2564
Size: 954 5239
MfeI C'AATT_G
Cuts at: 3792 3792
Size: 6193
MluI A'CGCG_T
Cuts at: 6069 6069
Size: 6193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3324 5307 5491 1305
Size: 2019 1983 184 2007
Fragments arranged by size:
2019 2007 1983 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2423 10
Size: 54 1425 343 591 3780
Fragments arranged by size:
3780 1425 591 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2351 4151 4510 4669 518
Size: 648 1185 1800 359 159 2042
Fragments arranged by size:
2042 1800 1185 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2167 2539 2713 3241 3255 3291 3609 4286
Size: 561 372 174 528 14 36 318 677
Cuts at: 4286 5434 5679 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 561 528 372 318 245
174 36 14
NaeI GCC'GGC
Cuts at: 2437 2740 3321 2437
Size: 303 581 5309
Fragments arranged by size:
5309 581 303
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5641
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6193
NgoMIV G'CCGG_C
Cuts at: 2435 2738 3319 2435
Size: 303 581 5309
Fragments arranged by size:
5309 581 303
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6193
NotI GC'GGCC_GC
Cuts at: 3640 3640
Size: 6193
NspI r_CATG'y
Cuts at: 1132 1229 3551 5096 1132
Size: 97 2322 1545 2229
Fragments arranged by size:
2322 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3267 1348
Size: 1919 4274
PciI A'CATG_T
Cuts at: 5092 5092
Size: 6193
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2200 3190 1108
Size: 1092 990 4111
Fragments arranged by size:
4111 1092 990
PfoI T'CCnGG_A
Cuts at: 2971 3116 3420 2971
Size: 145 304 5744
Fragments arranged by size:
5744 304 145
PmeI GTTT'AAAC
Cuts at: 3497 3497
Size: 6193
PmlI CAC'GTG
Cuts at: 2257 3168 2257
Size: 911 5282
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4262 4294 5775 5807 4262
Size: 32 1481 32 4648
Fragments arranged by size:
4648 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2047 2461 2519 3114 3372 3418 2047
Size: 414 58 595 258 46 4822
Fragments arranged by size:
4822 595 414 258 58 46
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6193
PsiI TTA'TAA
Cuts at: 3763 3763
Size: 6193
PspOMI G'GGCC_C
Cuts at: 2138 2935 3035 3283 2138
Size: 797 100 248 5048
Fragments arranged by size:
5048 797 248 100
PstI C_TGCA'G
Cuts at: 830 2020 3054 3545 830
Size: 1190 1034 491 3478
Fragments arranged by size:
3478 1190 1034 491
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6193
PvuII CAG'CTG
Cuts at: 2539 2713 3255 2539
Size: 174 542 5477
Fragments arranged by size:
5477 542 174
SacI G_AGCT'C
Cuts at: 2015 2015
Size: 6193
SalI G'TCGA_C
Cuts at: 1936 3535 1936
Size: 1599 4594
SanDI GG'GwC_CC
Cuts at: 2047 2519 2047
Size: 472 5721
SapI GCTCTTCn'nnn_
Cuts at: 2081 2081
Size: 6193
SbfI CC_TGCA'GG
Cuts at: 3545 3545
Size: 6193
ScaI AGT'ACT
Cuts at: 4441 6153 4441
Size: 1712 4481
SfcI C'TryA_G
Cuts at: 826 1045 2016 2641 3003 3050 3541 4676 5357
Size: 219 971 625 362 47 491 1135 681
Cuts at: 5357 5548 826
Size: 191 1471
Fragments arranged by size:
1471 1135 971 681 625 491 362 219
191 47
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6193
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6193
SmaI CCC'GGG
Cuts at: 1872 2137 2459 2941 3520 1872
Size: 265 322 482 579 4545
Fragments arranged by size:
4545 579 482 322 265
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4297 5198 5460 820
Size: 197 926 2354 901 262 1553
Fragments arranged by size:
2354 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3984 493
Size: 3491 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6193
SphI G_CATG'C
Cuts at: 1132 3551 1132
Size: 2419 3774
SspI AAT'ATT
Cuts at: 5 52 4117 5
Size: 47 4065 2081
Fragments arranged by size:
4065 2081 47
StuI AGG'CCT
Cuts at: 1517 2679 3091 1517
Size: 1162 412 4619
Fragments arranged by size:
4619 1162 412
StyI C'CwwG_G
Cuts at: 513 1065 2027 3457 3632 513
Size: 552 962 1430 175 3074
Fragments arranged by size:
3074 1430 962 552 175
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2957 3201 3259 4220 4237 4390 4575
Size: 1255 1377 244 58 961 17 153 185
Cuts at: 4575 5020 325
Size: 445 1498
Fragments arranged by size:
1498 1377 1255 961 445 244 185 153
58 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3177 3398 4439
Size: 274 80 33 51 1443 1200 221 1041
Cuts at: 4439 6151 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1200 1041 274 221 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2667 2699 3009 3041 6153 6185 2667
Size: 32 310 32 3112 32 2675
Fragments arranged by size:
3112 2675 310 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6193
XbaI T'CTAG_A
Cuts at: 3529 3529
Size: 6193
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2512 3029 2512
Size: 517 5676
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6193
XmaI C'CCGG_G
Cuts at: 1870 2135 2457 2939 3518 1870
Size: 265 322 482 579 4545
Fragments arranged by size:
4545 579 482 322 265
XmnI GAAnn'nnTTC
Cuts at: 4322 4322
Size: 6193
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4001 276
Size: 53 83 186 936 2467 2468
Fragments arranged by size:
2468 2467 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BfrBI BsiWI
BsmBI BstZ17I EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PspXI PsrI RsrII SacII SexAI
SfiI SfoI SrfI SwaI